• Journal of Soil and Groundwater Environment
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• v.16 no.6
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• pp.46-57
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• 2011

In this study, analytical solution of multip-species transport equations coupled with a first-order reaction network under constant concentration boundary condition or total flux boundary condition is obtained using similarity transformation approach of Clement et al. (2000). The study shows the schematic process about how multi-species transport equations with first-order sequential reaction network is transformed through the similarity transformation approach into independent and uncoupled single species transport equations with first-order reaction. The analytical solution was verified through the comparison with popular commercial programs such as 2DFATMIC and RT3D. The analytical solution can be utilized in nuclear waste sites where radioactive contaminants and their daughter products occur and in industrial complex cities where chlorinated solvent such as PCE, TCE, and its biodegradation products produces. In addition, it can help the verification of the developed numerical code.

• The Plant Pathology Journal
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• v.33 no.2
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• pp.184-192
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• 2017

Tylenchulus semipenetrans is an important and widespread plant-parasitic nematode of citrus worldwide and can cause citrus slow decline disease leading to significant reduction in tree growth and yield. Rapid and accurate detection of T. semipenetrans in soil is important for the disease forecasting and management. In this study, a loop-mediated isothermal amplification (LAMP) assay was developed to detect T. semipenetrans using DNA extracted from soil. A set of five primers was designed from the internal transcribed spacer region (ITS1) of rDNA, and was highly specific to T. semipenetrans. The LAMP reaction was performed at $63^{\circ}C$ for 60 min. The LAMP product was visualized directly in one reaction tube by adding SYBR Green I. The detection limit of the LAMP assay was $10^{-2}J2/0.5g$ of soil, which was 10 times more sensitive than conventional PCR ($10^{-1}J2/0.5g$ of soil). Examination of 24 field soil samples revealed that the LAMP assay was applicable to a range of soils infested naturally with T. semipenetrans, and the total assay time was less than 2.5 h. These results indicated that the developed LAMP assay is a simple, rapid, sensitive, specific and accurate technique for detection of T. semipenetrans in field soil, and contributes to the effective management of citrus slow decline disease.

• Journal of Ginseng Research
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• v.43 no.1
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• pp.1-9
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• 2019

Background: Korean ginseng is an important cash crop in Asian countries. However, plant yield is reduced by pathogens. Among the Ilyonectria radicicola-species complex, I. mors-panacis is responsible for root-rot and replant failure of ginseng in Asia. The development of new methods to reveal the existence of the pathogen before cultivation is started is essential. Therefore, a quantitative real-time polymerase chain reaction method was developed to detect and quantify the pathogen in ginseng soils. Methods: In this study, a species-specific histone H3 primer set was developed for the quantification of I. mors-panacis. The primer set was used on DNA from other microbes to evaluate its sensitivity and selectivity for I. mors-panacis DNA. Sterilized soil samples artificially infected with the pathogen at different concentrations were used to evaluate the ability of the primer set to detect the pathogen population in the soil DNA. Finally, the pathogen was quantified in many natural soil samples. Results: The designed primer set was found to be sensitive and selective for I. mors-panacis DNA. In artificially infected sterilized soil samples, using quantitative real-time polymerase chain reaction the estimated amount of template was positively correlated with the pathogen concentration in soil samples ($R^2=0.95$), disease severity index ($R^2=0.99$), and colony-forming units ($R^2=0.87$). In natural soils, the pathogen was recorded in most fields producing bad yields at a range of $5.82{\pm}2.35pg/g$ to $892.34{\pm}103.70pg/g$ of soil. Conclusion: According to these results, the proposed primer set is applicable for estimating soil quality before ginseng cultivation. This will contribute to disease management and crop protection in the future.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2004.04a
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• pp.332-336
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• 2004

An anaerobic PCE(tetrachloroethylene) dechlorinating bacterial culture from a landfill soil was enriched and characterized. The enrichment culture could dechlorinate 60$\mu$mol/$m\ell$ of PCE during a month of incubation and cis-DCE(cis-dichloroethylene) was observed as a main product of PCE dechlorination. Microbial analysis of the dechlorinating enrichment culture by rising PCR-DGGE (Polymerase chain reaction-Denaturing gradient gel electrophoresis) method showed that at least three microorganisms were related to the anaerobic PCE dechlorination.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2005.10a
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• pp.122-122
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• 2005

The remediation for contaminated soil and groundwater in contaminated site and waste site has to be compact and economic in maintaining and operating the system. In this study, the atomized slag was tested if they are an effective reactive material in permeable reactive barrier This novel reactive system technology was applied to the treatment of leachate from unplanned waste landfill. The system was optimized and developed to be commercialized.

• Proceedings of the Korean Geotechical Society Conference
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• 2000.03b
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• pp.255-262
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• 2000

Shaking table tests were peformed to evaluate the subgrade reaction of ground according to the build-up of pore water pressure. Model pile was installed in the sand ground. The acceleration of the model ground, the pore water pressure build-up and displacement of pile were recorded by measuring devices. Subgrade reaction approach based on Winker soil model was applied to obtain the modulus of the horizontal subgrade reaction. The results of analysis show that the reduction factor of the subgrade reaction due to pore pressure increase is about 1 and the horizontal subgrade reaction of liquefied ground is not influenced by the stiffness of pile, a ground acceleration and the intial ground density.

• Journal of Biosystems Engineering
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• v.39 no.1
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• pp.11-20
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• 2014

Purpose: Walking type cultivator used for weeding generated excessive handle vibration as well as bouncing motion depending on the weeding speed. This research was conducted to define a design factor of the rotary weeding blades for reducing soil reaction forces as well as hand vibration. Methods: The motion and forces acting on the rotary blades were reviewed to find out the most influencing parameter on hand vibration. The installation angle (IA) of the blade was selected and analyzed to determine the condition of no reaction force less. For removing the unnecessary upward soil reaction, the design factor theory of weeding blade was suggested based on geometrics and dynamics. For evaluation of design factor theory, the experiment in situ was performed base on ISO 5349:1. The vibration $a_{hv}$ and theoretical value $X_{MF}$ were compared with two groups that one was positive group ($X_{MF}$ > 0) and the other was negative group ($X_{MF}$ < 0). Results: $X_{MF}$ was derived from rotational velocity, forward velocity, disk diameter, weeding depth, blade's width and IA of blade. Two groups had significant difference (p < 0.05). In aspect of the group mean total exposure duration, positive group was 17.53% bigger than negative group. When disk radius 100, 150 and 200 mm, minimum IAs were $4{\sim}27^{\circ}$, $3{\sim}15^{\circ}$ and $2{\sim}10^{\circ}$, respectively. A spread sheet program which calculated XMF was developed by Excel 2013. Conclusions: According to this result, minimum IA of weeding blade for soil reaction reduction could be obtained. For reduction hand-arm vibration and power consumption, minimum IA is needed.

• Earthquakes and Structures
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• v.14 no.1
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• pp.85-94
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• 2018

The paper focuses on the seismic responses of a hyperbolic cooling tower resting on soil foundation represented by the three-parameter Vlasov elastic soil model. The three-parameter soil model eliminates the necessity of field testing to determine soil parameters such as reaction modulus and shear parameter. These parameters are calculated using an iterative procedure depending on the soil surface vertical deformation profile in the model. The soil and tower system are modeled in SAP2000 structural analysis program using a computing tool coded in MATLAB. The tool provides a two-way data transfer between SAP2000 and MATLAB with the help of Open Application Programming Interface (OAPI) feature of SAP2000. The response spectrum analyses of the tower system with circular V-shaped supporting columns and annular raft foundation on elastic soil are conducted thanks to the coded tool. The shell and column forces and displacements are presented for different soil conditions and fixed raft base condition to investigate the effects of soil-structure interaction. Numerical results indicate that the flexibility of soil foundation leads to an increase in displacements but a decrease in shell membrane and column forces. Therefore, it can be stated that the consideration of soil-structure interaction in the seismic response analysis of the cooling tower system provides an economical design process.

• The Plant Pathology Journal
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• v.26 no.2
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• pp.198-202
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• 2010

A study was conducted to find out the relationship of physico-chemical properties (viz. organic carbon(OC), pH, electrical conductivity, nitrogen, phosphorus and potassium content) of ginger growing soil with incidence percentage of rhizome rot and wilt disease complex of ginger. Organic carbon content and pH of the ginger soil contributed significantly (93%) in the prediction of ginger rhizome rot and wilt disease complex incidence with negative correlation. Soil having weak acidic reaction with OC percent greater than 2.25 was observed to have the lower average incidence of the disease.

• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 2003.10a
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• pp.559-562
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• 2003

The results and discussions of surveyed case site at constructed steel pile in potential acid sulfate soil were as follows. Topography at surveyed site was local alluvial valley and that site soils was classified as BanGog and YuGye series as detailed soil surveyed results in RDA and soil texture was Clay/Clay Loam. Soils pH was neutral, which was average 7.5 but much decreased to average 4.2 after $H_2O_2$ treatment. Organic matter and sulfate ions contents were very rich. The corrosion was severe at ground water fluctuation depth. Deposits colored black were attached to steel pile surface, which because of violent reaction in treatment HCI solution, were guessed as corrosion products (FeS) reduced by sulfate reducing bacteria(SRB). Consequently, main cause was thought microbiologically induced corrosion at this site where there is ground water fluctuation occurring oxidation and reduction reactions in turn and the soil is potential acid sulfate soil.