• Journal of Microbiology
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• 제43권1호
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• pp.28-33
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• 2005

The purpose of this study was to develop a multipurpose incubator, without the gas cylinders (bottles) which are required for $H_2$ and $CO_2$ supplementation. In our bottle-free multipurpose incubator, the $H_2$ and $CO_2$ were generated by chemical reactions induced within the chamber. The reaction between sodium borohydride and acetic acid at a molar ratio of 1:1 was used to generate $H_2$, according to the following formula: $4NaBH_4+2CH_3COOH+7H_2O{\rightarrow} 2CH_3COONa+Na_2B_4O_7+16H_2$, whereas the other reaction, citric acid and sodium bicarbonate at a 1:1 molar ratio, was used to generate $CO_2$, according to the following formula: $C_6H_8O_7+3NaHCO_3{\rightarrow}Na_3(C_6H_5O_7)+3H_2O+3CO_2$. Five species of obligate anaerobic bacteria, one strain of capnophilic bacterium, and one strain of microaerophilic bacterium were successfully cultured in the presence of their respective suitable conditions, all of which were successfully generated by our bottle-free multipurpose incubator. We conclude that, due to its greater safety, versatility, and significantly lower operating costs, this bottle-free multipurpose incubator can be used for the production of fastidious bacterial cultures, and constitutes a favorable step above existing anaerobic incubators.

• Korean Chemical Engineering Research
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• 제53권5호
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• pp.590-596
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• 2015

본 연구에서는 다양한 $CO_2$ 재활용 기술 중 경제성 및 $CO_2$ 감축량 효과가 큰 것으로 평가되는 $CO_2$ 활용 중탄산나트륨 제조기술 대상으로 상용 플랜트 운영시 전체 $CO_2$ 감축량을 산정하고자 하였다. 상기 $CO_2$ 재활용 기술은 발전소 배가스 중에 포함된 $CO_2$의 탄산화 반응을 통해 상업적으로 유용한 중탄산나트륨을 제조하는 기술로서 현재 한국동서 발전의 지원을 받아 한전 전력연구원에서 연구개발 진행 중이다(기술개발 사업명: NCCU, Non-Capture $CO_2$ Utilization). 본 기술의 $CO_2$ 감축량 산정을 위해 하루 100톤 $CO_2$ 처리 규모(연간 36,500톤 $CO_2$ 처리 가능, 발전 용량 기준 5 MW급)의 상용급 플랜트를 대상으로 공정모사 프로그램(PRO/II 9.1)을 활용한 열 및 물질 수지 분석을 수행하였으며 특히 종래 유사기술과의 비교를 통한 간접 $CO_2$ 감축량 산정을 위해 탄산나트륨 및 중탄산나트륨 등의 제조를 위한 대표적 기술인 Solvay 공정과의 에너지 사용량을 비교 분석하였다. 분석 결과 종래 Solvay 공정은 단위 중탄산나트륨 생산을 위한 에너지 사용량이 약 $7.4GJ/tNaHCO_3$으로 이를 해당 에너지를 얻기 위해 필요한 석탄 사용량 및 $CO_2$ 발생량으로 환산시 연간 약 48,862 톤 $CO_2$에 해당 된다. 반면 발전소 배가스 중에 포함된 $CO_2$를 활용한 중탄산나트륨 제조공정의 경우 탄산화 반응에 의한 $CO_2$ 직접 포집분(연간 약 36,500 톤)과 동일 화합물 생산을 위한 종래 공정(Solvay) 대비 낮은 에너지 사용량에 따른 간접적인 $CO_2$ 저감량(연간 약 46,885 톤) 효과로 전체 $CO_2$ 감축량은 약 83,385톤으로 산정되었다. 상기 분석을 통해 본 논문의 $CO_2$ 활용 중탄산나트륨 제조기술은 제품 판매에 따른 경제적 효과뿐만 아니라 종래 공정에 비해 낮은 에너지 사용으로 $CO_2$ 저감효과가 매우 높아 대규모 $CO_2$ 저장 공간이 필요한 CCS(Carbon Capture & Sequestration) 기술의 대안기술로서 유망한 것으로 분석되었다.

• 한국임상수의학회지
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• 제18권1호
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• pp.74-77
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• 2001

A ten-year-old, male cat presented with recent loss of body weight, depression, vomiting, anorexia, polydipsia, and polyuria. General physical findings included depression, weakness, severe dehydration and a strong acetone odor on the breath. A complete blood count and serum biochemical profiles were leukocytosis, hyperglycemia (286 mg/dl), hypokalemia (2.6 mEq/L), hyponatremia, and high serum fructosamine (600 $\mu$mol/L). In blood gas analysis the cat had acidosis (pH 7.127, p$CO_2$26.7 mmHg). In urinalysis glycosuria and ketouria were appeared. On the basis of clinical signs, serum chemistry, blood gas analysis and urinalysis, diabetic ketoacidosis was diagnosed. Treatment included subcutaneous administration of protamine zinc insulin (0.75 U/head) and intravenous administration of 0.9% saline. Potassium phosphate and sodium bicarbonate was added to the fluids. Serum fructosamine for assessment of glycemic control was measured on occasion calls. On day 296, the patient improved clinically and did not experience any problems resulting from diabetic ketoacidosis.

• Journal of Microbiology and Biotechnology
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• 제3권1호
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• pp.19-23
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• 1993

Spirulina platensis NIES 39 was grown in effluents from a wastewater treatment plant utilizing activated sludge process at a pig farm to reduce pollutants and to produce feed resources. The addition of 2 g/l NaCl was required for the growth of S. platensis in the effluents with about 100 mg/l ammonia nitrogen concentration. The growth was stimulated by the addition of 2 g/l sodium bicarbonate and 4 mg/l ferrous sulfate. The microorganism grew well at pH 8~11, and the optimum pH for the growth was 10. The algal concentration of 1.1 g/l was attained after 8 days of growth at the optimum condition with the removal of 95% ammonia nitrogen and 34% phosphorus.

• 한국미생물·생명공학회지
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• 제21권4호
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• pp.381-391
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• 1993

As the first stage of development of an artificial skin, fibroblasts were cultured in the collagen matrices to make a living dermal equivalent. Mouse embryonic fibroblasts were incorporated into a collagen matrices on plastic dishes containing concentrated DMEM culture media supplemented with sodium bicarbonate, hepes, antibiotics and fetal bovine serum. As the growth stimulation components, glycosaminoglycans were added: hyaluronic acid, chondroitin sulfate, heparin, chitosan were incorporated into the media at a concentration of either 1% or 5% w/w/ to collagen in order to investigate the effect on development of dermal equivalent. After the few days of incubation, gel matrics were contracted and firm dermal equivalent were formed. And the keratinocytes were cultured on top of dermal equivalent and make a three dimensional artificial skin tissue.

• Journal of Chest Surgery
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• 제10권2호
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• pp.250-267
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• 1977

Open heart surgery has been performed on 20 patients, using hemodilution principle under the moderate hypothermia from Dec. 1975 through Aug. 1977 at the Department of Thoracic and Cardiovascular Surgery, Kyungpook National University School of Medicine. All these patients, body surface area ranged from 0.53 to 1.67$M^2$, were divided into two groups as a child group [below 15 yrs] and a adult group[above 15 yrs]. The oxygenator were primed with fresh ACD blood, 5 per cent dextrose, Hartmann`s solution, 15 per cent mannitol, sodium bicarbonate, dexamethasone and antibiotics. The average flow rate was 2.0 L/$M^2$/min. in child group and 2.3L/$M^2$/min. in adult group. The degree of hemodilution in child and adult group was 30.7% and 29.3% respectively. The minimal value of rectal temperature was $30.84{\pm}0.7^{\circ}C.$, in child group and $30.0{\pm}1.5^{\circ}C.$, in adult group. We studied the changes of hemodynamic status, blood components, electrolyte, acid-base status. blood lactic acid and urine output during and after cardiopulmonary bypass.

• 약학회지
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• 제31권2호
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• pp.60-63
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• 1987

The effect of manufacturing and packaging conditions on the stability of effervescent tablets has been studied. Mixtures of sodium bicarbonate and tartaric acid were compressed at tabletting forces of 3,6, and 9 ton force. Tablets were more than simple physical mixture but the effect of tabletting force has not been found in this force range. "Curing" the tablet by heating it to $90^{\circ}C$ was effective on increasing the stability and the silica gel in the aluminium pouch stabilized the system. The equipment measuring the carbon dioxide pressure in this experiment was very useful in evaluation of the effervescent tablets.t tablets.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 1986년도 추계학술대회
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• pp.532.2-532
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• 1986

Alkaline protease which is an important enzyme used in detergents, leather tanning and food industry was produced by alkalophilic bacterium, Bacillus sp. KCTC 1723 isolated from soil. The maximum productivity of the enzyme in alkaline medium containing 1% sodium bicarbonate was obtained by incubating for 3 days at 37$^{\circ}C$. The optimum pH of the enzyme was 11.5 and calcium ion was effective on stabilization of the enzyme at high temperature. The enzyme was not inhibited by metal chelating agent such as El)TA but inhibited by diisopropyl fluorophosphate. Purification of the enzyme was carried out DEAE- and CM-cellulose column chromatographies and molecular weight of the purified enzyme was determined

• 대한임상독성학회지
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• 제3권1호
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• pp.63-66
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• 2005

Toluene is an aromatic hydrocarbon found in glues, cements, and solvents. It is known to be toxic to the nervous system, hematopoietic system, and causes acid-base and electrolyte disorders. Acute respiratory failure with hypokalemia and rhabdomyolysis with acute renal failure should be considered as potential events in protracted glue sniffing. We reported the case of 26-year-old woman was admitted to the emergency department with the development of respiratory failure and altered mentality due to hypokalemia after chronic glue sniffing. She was weaned from the ventilator 3 days later after potassium and sodium bicarbonate replacement and was discharged without respiratory symptoms and other complications.

• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2003년도 추계학술대회초록집
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• pp.6-6
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• 2003

Listeria monocytogenes is a facultative bacterium that cause severe clinical disease including meningoencephalitis, septicaemia, and abortion in pregnant women, newborn infants, the debilitated elderly or immunocompromised people. In preliminary experiments on murine listeriosis we noticed suppurative gastritis in mice infected with L. monocytogenes by the intragastric route. The aims of the present study were ⅰ) to describe the histopathology of the experimentally listeria-induced gastroenteritis ⅱ) to investigate the influence of bacterial strain and laboratory mouse strain on infectivity and on the severity of the infection; [3] to examine possible effects of preliminary intragastric administration of sodium bicarbonate. (omitted)