• Journal of Interdisciplinary Genomics
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• 제4권2호
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• pp.35-38
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• 2022

Background: Prader-Willi syndrome (PWS) is a complex genetic disease associated with growth impairment, severe obesity and metabolic dysfunctions. High proportion of PWS patients are born small for gestational age (SGA) than normal children, which also increase the risk of growth impairment and metabolic dysfunction in PWS. We aimed to compare growth outcome and metabolic profiles between SGA and appropriate for gestational age (AGA) PWS patients. Methods: Data of 55 PWS children and adults aged more than 2 years old (32 male and 23 female, age 2-18.8 years) from single center were studied. Only patients who were treated with GH were included. The clinical characteristics and laboratory findings were reviewed retrospectively. Results: Among 55 subjects, 39 had 15q11-13 deletion and 16 had uniparental disomy (UPD). Twenty (36.3%) were born SGA. All patients received GH treatment, and 11 (20%) discontinued GH treatment. Mean age at GH treatment initiation was 2.5 (range 0.3-12.4) years, and mean duration of treatment was 6.3 (range 1.0-11.3) years. Current height-SDS (-0.36 vs -0.16) and BMI-SDS (1.44 vs 1.33) did not differ between AGA and SGA group. Two patients in SGA group, but none in AGA group had diabetes mellitus. Mean glucose level was also higher in SGA group (100.1 vs 114.4 mg/dL). Conclusion: Our report gives an overview of growth profile and metabolic dysfunctions recorded in GH treated PWS patients. Growth profile did not differ between AGA and SGA group. Glucose level was higher in SGA group, so more careful monitoring and prevention for DM will be required in SGA group.

• 한국응용과학기술학회지
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• 제39권5호
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• pp.632-643
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• 2022

Although probiotics have been shown to improve health when consumed, recent studies have reported that they can cause unwanted side effects due to bacterial-human interactions. Therefore, the importance of prebiotics that can form beneficial microbiome in the gut has been emphasized. This study isolated and identified bacteria capable of producing biopoymer as a candidate prebiotic from traditional fermented foods. The isolated and identified strain was named WCYSK01 (Wissella sp. strain YSK01). The composition of the medium for culturing this strain was prepared by dissolving 3 g K₂HPO₄, 0.2 g MgSO₄, 0.05 g CaCl₂, 0.1 g NaCl in 1 L of distilled water. The LMBP(low molecular weight biopoymers) produced when fermentation was performed with sucrose and maltose as substrates were mainly consisted of DP3 (degree of polymer; isomaltotriose), DP4 (isomaltotetraose), DP5 (isomaltopentaose), and DP6 (isomaltoheptaose). The optimization of LMBP (low molecular weight of biopolymer) production was performed using the response surface methodology. The fermentation process temperature range of 18 to 32℃, the fermentation medium pH in the range of 5.1 to 7.9. The yield of LMBP production by the strain was found to be significantly affected by q fermentation temperature and pH. The optimal fermentation conditions were found at the normal point, and the production yield was more than 75% at pH 7.5 and temperature of 23℃.

• Journal of Microbiology and Biotechnology
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• 제32권9호
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• pp.1110-1119
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• 2022

Fe-S clusters are versatile and essential cofactors that participate in multiple and fundamental biological processes. In Escherichia coli, the biogenesis of these cofactors requires either the housekeeping Isc pathway, or the stress-induced Suf pathway which plays a general role under conditions of oxidative stress or iron limitation. In the present work, the Fe-S cluster assembly Isc and Suf systems of acidophilic Bacteria and Archaea, which thrive in highly oxidative environments, were studied. This analysis revealed that acidophilic microorganisms have a complete set of genes encoding for a single system (either Suf or Isc). In acidophilic Proteobacteria and Nitrospirae, a complete set of isc genes (iscRSUAX-hscBA-fdx), but not genes coding for the Suf system, was detected. The activity of the Isc system was studied in Leptospirillum sp. CF-1 (Nitrospirae). RT-PCR experiments showed that eight candidate genes were co-transcribed and conform the isc operon in this strain. Additionally, RT-qPCR assays showed that the expression of the iscS gene was significantly up-regulated in cells exposed to oxidative stress imposed by 260 mM Fe₂(SO₄)₃ for 1 h or iron starvation for 3 h. The activity of cysteine desulfurase (IscS) in CF-1 cell extracts was also upregulated under such conditions. Thus, the Isc system from Leptospirillum sp. CF-1 seems to play an active role in stressful environments. These results contribute to a better understanding of the distribution and role of Fe-S cluster protein biogenesis systems in organisms that thrive in extreme environmental conditions.

• 한국정보통신학회논문지
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• 제27권1호
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• pp.76-86
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• 2023

항공기에 탑재되는 레이다는 연속적으로 I/Q 데이터를 획득하는 동안 주어진 시간 내에 신호처리를 완료하는 실시간성을 반드시 유지해야 하며, 공대공부터 공대지, 공대해, Ground Map까지 다양한 모드와 기능을 수행해야 하는 특징을 가지고 있다. 따라서 개발이 진행될수록 레이다 신호처리 SW를 구성하는 코드의 구조가 복잡해진다. 또한 여러 모드를 순차적으로 구현하는 과정에서 기존 코드를 재활용하기도 하고 신호처리 알고리즘의 일부분만 바뀌기도 한다. 이와 같은 이유로 실시간성을 유지하며 코드의 재사용과 부분 교체가 편리한 모듈화의 장점을 이용해야 할 필요가 있다. 본 논문에서는 공대지/해를 구성하는 모드의 신호처리 과정을 통해, 실시간성을 유지하며 항공기 레이다 신호처리 SW에 적용 가능한 모듈화 Framework의 설계 방안을 제시한다.

• Journal of Ginseng Research
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• 제46권3호
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• pp.464-472
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• 2022

Background: Gut microbiota influence the central nervous system through gut-brain-axis. They also affect the neurological disorders. Gut microbiota differs in patients with Alzheimer's disease (AD), as a potential factor that leads to progression of AD. Oral intake of Korean Red Ginseng (KRG) improves the cognitive functions. Therefore, it can be proposed that KRG affect the microbiota on the gut-brain-axis to the brain. Methods: Tg2576 were used for the experimental model of AD. They were divided into four groups: wild type (n = 6), AD mice (n = 6), AD mice with 30 mg/kg/day (n = 6) or 100 mg/kg/day (n = 6) of KRG. Following two weeks, changes in gut microbiota were analyzed by Illumina HiSeq4000 platform 16S gene sequencing. Microglial activation were evaluated by quantitative Western blot analyses of Iba-1 protein. Claudin-5, occludin, laminin and CD13 assay were conducted for Blood-brain barrier (BBB) integrity. Amyloid beta (Aβ) accumulation demonstrated through Aβ 42/40 ratio was accessed by ELISA, and cognition were monitored by Novel object location test. Results: KRG improved the cognitive behavior of mice (30 mg/kg/day p < 0.05; 100 mg/kg/day p < 0.01), and decreased Aβ 42/40 ratio (p < 0.01) indicating reduced Aβ accumulation. Increased Iba-1 (p < 0.001) for reduced microglial activation, and upregulation of Claudin-5 (p < 0.05) for decreased BBB permeability were shown. In particular, diversity of gut microbiota was altered (30 mg/kg/day q-value<0.05), showing increased population of Lactobacillus species. (30 mg/kg/day 411%; 100 mg/kg/day 1040%). Conclusions: KRG administration showed the Lactobacillus dominance in the gut microbiota. Improvement of AD pathology by KRG can be medicated through gut-brain axis in mice model of AD.

• 한국염색가공학회지
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• 제35권1호
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• pp.8-19
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• 2023

Silicone rubber is widely used in most industries due to diverse advantages like heat stability, UV stability, durability, chemical resistance, environment friendliness, inertness and so on. But there is limitation to expand applications due to relatively weak rubber strengths such as tensile strength and tear strength, especially in fabric coating applications. The purpose of this study is to find influence of coating agent on performances of rubber and coated fabrics and their correlation according to different crosslinking densities of silicone rubbers. Addition cure type of silicones were formulated using crosslinked MQ-type silicone resin consisting of M (R₃SiO_1/2) and Q (SiO_4/2) and linear polymers. Raw materials used were; 1) linear vinyl endblocked polymers and vinyl functional MQ resin as main polymers, 2) linear silicone hydride polymers as crosslinkers, 3) platinum catalyst and 4) inhibitor to control curing speed. Rubber specimens were prepared to check mechanical strength using universal testing machine (UTM). Crosslinking density was calculated using Flory-Rhener equation using solvent swelling method. Differential scanning calorimetry (DSC) and scanning electron microscope (SEM-EDS) were used to characterize rubbers. Consequently, it was found that physical properties of silicone rubbers and coated fabrics can be expected by crosslinking density of rubbers. Silicone rubber formulations that contain 20 ~ 30 wt% of vinyl MQ resin showed strongest balanced performances.

• Medical Lasers
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• 제8권1호
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• pp.1-6
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• 2019

Background and Objectives A facial laceration is a commonly occurring trauma of children. In addition, appropriate treatment and scar management are very important because the psychosocial impact of facial scars is much greater in children than in adults. Materials and Methods In the authors' hospital, primary closure was conducted targeting a total of 8,924 patients from January 2015 to December 2017. Of these, scar management was conducted on 808 people using a CO₂ fractional laser and Q-Switch Nd:YAG laser. To prove the effectiveness of the laser treatment on the linear scar, the patients were divided retrospectively into children and adults and sub-divided into those who received the treatment and those who did not. The subjective satisfaction of patients was evaluated using the Numeric Rating System (NRS). The objective scar scoring was evaluated using the image panel assessment based on the Vancouver Scar Scale (VSS), and the scoring was made by averaging the evaluations of three plastic surgeons. Results Evaluated NRS showed 8.50 ± 1.83 for adults without a laser, 8.51 ± 1.47 for adults with a laser, 7.93 ± 2.10 for children without a laser, and 8.9 ± 1.24 for children with a laser. The image panel assessment revealed 7.06 ± 1.31 for adults without a laser, 6.40 ± 2.37 for adults with a laser, 6.86 ± 1.45 for children without a laser, and 5.93 ± 1.21 for children with a laser. Conclusion When the linear scar is managed using a CO₂ Fractional laser, it can be managed in children better than adults, which is believed to be useful because it leads to better satisfaction and prognosis.

• 예술인문사회 융합 멀티미디어 논문지
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• 제8권6호
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• pp.417-426
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• 2018

본 연구의 목적은 외국인에게 중국어를 가르칠 수 있는 중국어 교사의 역량에 관한 현직 중국어 교사의 인식을 알아봄으로써 개선 방향을 제시하고자 한다. 따라서 본 연구는 중국 산동성 Q시에 외국인에게 중국어를 가르치고 있는 중국어 교사들 56명을 대상으로 설문조사를 실시하였다. 중국어 교사로서의 역량에 대해 그 중요정도는 중국어 교사들이 비교적인 높은 평균값으로 인식하고 있다는 것으로 나타났지만, 실제 보유정도는 평균 점수가 비교적인 낮은 것으로 나타냈다. 이런 결과를 바탕으로 내린 결론은 다음과 같다. 외국인에게 중국어를 가르칠 수 있는 중국어 교사의 역량이 시식, 기술, 소양 3가지 요소로 여길 수 있다. 중국어 교사 역량을 높이기 위하여 중국 문화에 관한 지식, 교수-학습 방법과 기능, 다문화 적응, 교학 연구 등 측면에 편중하게 노력해야 할 필요가 있다. 앞으로 중국어 교사 양성 및 연수 프로그램에서 중국 문화에 대한 지식 학습을 강화할 뿐만 아니라, 언어 교육의 다양한 교수-학습 방법에 대한 학습을 중시해야 한다. 양호한 심리적 소양을 유지하면서 다문화 적응 능력도 향상시켜야 한다.

• 천문학회보
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• 제45권1호
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• pp.61.4-62
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• 2020

We present H I gas kinematics and star formation activities of NGC 6822, a dwarf galaxy located in the Local Volume at a distance of ~490 kpc. We perform profile decomposition of the line-of-sight velocity profiles of the high-resolution (~42.4" × 12") spatial; ~1.6 km/s spectral) H I data cube taken with the Australia Telescope Compact Array (ATCA). For this, we use a new tool, the so-called BAYGAUD (BAYesian GAUssian Decompositor) which is based on Bayesian Markov Chain Monte Carlo (MCMC) techniques, allowing us to decompose a line-of-sight velocity profile into an optimal number of Gaussian components in a quantitative manner. We classify the decomposed H I gas components of NGC 6822 into kinematically cold, warm or hot ones with respect to their velocity dispersion: 1) cold: < 4 km/s, 2) warm: 4 ~ 8 km/s, 3) hot: > 8 km/s. We then derive the Toomre-Q parameters of NGC 6822 using the kinematically decomposed H I gas maps. We also correlate their gas surface densities with the surface star formation rates derived using both GALEX far-ultraviolet and WISE 22 micron data to examine the impact of gas turbulence caused by stellar feedback on the Kennicutt-Schmidt (K-S) law. The kinematically cold component is likely to better follow the linear extension of the Kennicutt-Schmidt (K-S) law for molecular hydrogen (H2) at the low gas surface density regime where H I is not saturated.

• 실천공학교육논문지
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• 제15권1호
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• pp.39-44
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• 2023

이러닝 교수법의 발전에 따라 대면교육과 이러닝을 혼합한 블렌디드 러닝의 요구가 높아지고 있으며 기존의 대면수업을 대체할 만한 학습효과를 보여주고 있다. 그러나 공학 교과목은 실습과 같은 다양한 학습활동이 이루어지고 있어 기존의 전통적인 블렌디드 러닝으로 운영하기 쉽지 않다. 따라서 공학 교과목에 필요한 학습활동에 따라 기존과는 다른 교수학습설계가 필요로 된다. 본 논문에서는 공학교과목에서 블렌디드 러닝을 위해 교수법 설계 및 운영사례를 소개하고 그 효과를 조사 분석하였다. 학습활동을 이론수업, 실습수업, 퀴즈 및 질의응답, 과제수행 및 풀이 등으로 세분화하고 학습활동에 따라 온라인 동영상, LMS 활용, 대면수업의 교수 학습방법을 적용하였다. 학생들의 수업만족도 조사결과를 보면 공학교과목에서도 블렌디드 러닝은 순수온라인과 대면수업에 비해 만족도가 높게 나타났으며 학습활동별로 차별화된 만족도를 나타내었다.