• Journal of Microbiology
• /
• v.35 no.3
• /
• pp.234-238
• /
• 1997

Conditional lethal mutation nup49-1 of a nuclear pore complex component gene was constructed in Saccharomyces cerevisiae. This mutation deleted one third of the essential NUP49 gene at the carboxy-terminal, but retained 13 repeats of the highly conserved GLFG domain. The nup49-1 mutant strain was viable with a slow-growth phenotype, indicating that the C-terminal is dispensable at normal growth temperature. This strain exhibited both temperature-sensitivity at 37.deg.C and cold-sensitivity at 16.deg.C. Temperature shift experiments revealed that the arrest phenotype at 37.deg.C was random in the cell division cycle. The nup49-1 mutation was tested to be recessive and is expected to be useful for the functional analysis of nuclear pore complex proteins as well as for studies of nuclear transport systems.

• Journal of Life Science
• /
• v.15 no.2 s.69
• /
• pp.253-260
• /
• 2005

Mitotic centromere-associated kinesin (MCAK), which is a member of the Kin I (internal motor domain) subfamily of kinesin-related proteins, is known to play a role in mitotic segregation of chromosome during M phase of the cell cycle. In the present study, we have produced a rat polyclonal antibody using human MCAK (HsMCAK) expressed in E. coli as the antigen. The antibody specifically recognized the HsMCAK protein (81 kDa), and could detect its nuclear localization in human Jurkat T cells and 293T cells by Western blot analysis. The specific stage of the cell cycle was obtained through blocking by either hydroxyl urea or nocodazole and subsequent releasing from each blocking for 2, 4, and 7 h. While the protein level of HsMCAK reached a maximum level in the S phase with slight decline in the $G_{2}-M$ phase, the electrophoretic mobility shift from $p81^{MCAK}\;to\;p84^{MCAK}$ began to be induced in the late S phase and reached a maximum level in the $G_{2}/M $ phase, and then it disappeared as the cells enter into the $G_{1}$ phase. Immunocytochemical analysis revealed that HsMCAK protein localized to centrosome and nucleus at the interphase, whereas it appeared to localize to the spindle pole, centromere of the condensed mitotic DNA, spindle fiber, or midbody, depending on the specific stage of the M phase. These results demonstrate that a rat polyclonal antibody raised against recombinant HsMCAK expressed in E. coli specifically detects human MCAK, and indicate that the electrophoretic mobility shift from $p81^{MCAK}\;to\;p84^{MCAK}$, which may be associated with its differential intracellular localization during the cell cycle, fluctuates with a maximum level of the shift at the $G_{2}-M$ phase.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2022.05a
• /
• pp.436-436
• /
• 2022

셀룰러 오토마타(Cellular Automata; CA)는 격자(cell)에 대해 사전 정의된 규칙을 바탕으로 이웃 격자 간 상호작용을 해석하여 복잡한 동력학적 현상을 효과적으로 재현할 수 있는 이산형(discrete) 모의 기법이다. CA 기법은 격자 구조에 수치표고 자료 및 토양수분 정보 등을 직접 매칭 후 상호관계를 해석하기 때문에 공간정보를 최대한 활용하여 불균질성을 나타내는 것이 가능하다. 따라서, 도시 유출해석에 있어서 높은 정확도와 빠른 계산속도를 기대할 수 있다. 본 연구에서는 CA 기반 고해상도 물순환·침수 연계 해석 framework 개발 방향 및 CA 기반 prototype 모형의 사면유출 적용 사례를 소개한다. 개발 중인 CA 모형에서는 격자별 침수 깊이, 침투, 토양수분 저류, 지표 유출 등의 물순환 요소를 모의할 수 있다. 기존의 집중형(lumped) 모형은 지표-지표하 유출에 대한 routing algorithm이 없고 각 셀의 물수지 모형 내 파라미터가 많은 단점이 있다. 따라서 개발 중인 CA 모형에서는 cell state 내 fast reservoir와 slow reservoir를 통해 지표-지표하 상태를 구현하고 단순화된 물수지 모형 및 흐름 방향 알고리즘을 적용함으로써 실제 현장에서 발생하는 다중 피크 형태의 지표 유출을 모사한다. 최적의 지표수 흐름 방향 알고리즘 선정을 위해 3개의 다중 흐름 방향 알고리즘(D4, D8, 4+4N)을 정량적으로 비교·분석한다. 이번 발표에서는 CA 모형을 소규모 산지 사면과 도심지 등 다양한 규모의 테스트베드에 적용하여 모형의 장단점을 평가한다.

• Korean Journal of Remote Sensing
• /
• v.29 no.4
• /
• pp.423-441
• /
• 2013

On 21 September 2010, one of Chuseok holidays in Korea, localized heavy rainfalls occurred over the midwestern region of the Korean peninsula. In this study MTSAT-2 infrared and water vapor channel imagery are examined to find out some features which are obvious in each stage of the life cycle of convective cell for this heavy rain event. Also the kinematic and thermodynamic features probably associated with them are investigated. The first clouds related with the Chuseok heavy rain are detected as low-level multicell cloud (brightness temperature: $-15{\sim}0^{\circ}C$) in the middle of the Yellow sea at 1630~1900 UTC on 20 Sept., which are probably associated with the convergence at 1000 hPa. Convective cells are initiated in the vicinity of Shantung peninsula at 1933 UTC 20, which have developed around the edge of the dark region in water vapor images. At two times of 0033 and 0433 UTC 21 the merging of two convective cells happens near midwestern coast of the peninsula and then they have developed rapidly. From 0430 to 1000 UTC 21, key features of convective cell include repeated formation of secondary cell, slow horizontal cloud motion, persistence of lower brightness temperature ($-75{\sim}-65^{\circ}C$), and relatively small cloud size (${\leq}-50^{\circ}C$) of about $30,000km^2$. Radar analysis showed that this heavy rain is featured by a narrow line-shaped rainband with locally heavy rainrate (${\geq}50$ mm/hr), which is located in the south-western edge of the convective cell. However there are no distinct features in the associated synoptic-scale dynamic forcing. After 1000 UTC 21 the convective cell grows up quickly in cloud size and then is dissipated. These satellite features may be employed for very short range forecast and nowcasting of mesoscale heavy rain system.

• Journal of Microbiology and Biotechnology
• /
• v.23 no.11
• /
• pp.1586-1597
• /
• 2013

Energy-efficient metabolic responses were often noted in high-productive cultures. To better understand these metabolic responses, an investigation into the relationship between metabolic responses and energy regulation was conducted via a comparative analysis among cultures with different energy source supplies. Both glycolysis and glutaminolysis were studied through the kinetic analyses of major extracellular metabolites concerning the fast and slow cell growth stages, respectively, as well as the time-course profiles of intracellular metabolites. In three cultures showing distinct antibody productivities, the amino acid metabolism and energy state were further examined. Both the transition of lactate from production to consumption and steady intracellular pools of pyruvate and lactate were observed to be correlated with efficient energy regulation. In addition, an efficient utilization of amino acids as the replenishment for the TCA cycle was also found in the cultures with upregulated energy metabolism. It was further revealed that the inefficient energy regulation would cause low cell productivity based on the comparative analysis of cell growth and productivity in cultures having distinct energy regulation.

• Nuclear Engineering and Technology
• /
• v.52 no.11
• /
• pp.2431-2441
• /
• 2020

Currently, the pin-by-pin homogenized solvers are a very active research field as they can, unlike the nodal codes, directly predict the local power, while requiring significantly less computational resources than the heterogeneous transport codes. This paper presents a recently developed pin-by-pin diffusion/SP3 solver Tortin, its spatial discretization method and the reflector treatment. Regarding the spatial discretization, it was observed that the finite difference method applied on pin-cell size mesh does not properly capture the big flux change between MOX and uranium fuel, while the nodal expansion method is more accurate but too slow. If the finite difference method is used with a finer mesh in the outer two pin rows of the fuel assembly, it increases the required computation time by only 50%, but decreases the pin power errors below 1% with respect to lattice code reference solutions. The paper further describes the coupling of Tortin with a microscopic depletion solver. Several verification tests show that the SP3 pin-by-pin solver can reproduce the heterogeneous transport solvers results with very good accuracy, even for fuel cycle depletion of very heterogeneous core employing MOX fuel or inserted control rods, while being two orders of magnitude faster.

• 한국정보디스플레이학회:학술대회논문집
• /
• 2008.10a
• /
• pp.993-994
• /
• 2008

Electrochromic (EC) devices are capable of reversibly changing their optical properties upon charge injection and extraction induced by the external voltage. The characteristics of the EC device, such as low power consumption, high coloration efficiency, and memory effects under open circuit status, make them suitable for use in a variety of applications including smart windows and electronic papers. Coloration due to reduction or oxidation of redox chromophores can be used for EC devices (e-paper), but the switching time is slow (second level). Recently, with increasing demand for the low cost, lightweight flat panel display with paper-like readability (electronic paper), an EC display technology based on dye-modified $TiO_2$ nanoparticle electrode was developed. A well known organic dye molecule, viologen, was adsorbed on the surface of a mesoporous $TiO_2$ nanoparticle film to form the EC electrode. On the other hand, ZnO is a wide bandgap II-VI semiconductor which has been applied in many fields such as UV lasers, field effect transistors and transparent conductors. The bandgap of the bulk ZnO is about 3.37 eV, which is close to that of the $TiO_2$ (3.4 eV). As a traditional transparent conductor, ZnO has excellent electron transport properties, even in ZnO nanoparticle films. In the past few years, one-dimension (1D) nanostructures of ZnO have attracted extensive research interest. In particular, 1D ZnO nanowires renders much better electron transportation capability by providing a direct conduction path for electron transport and greatly reducing the number of grain boundaries. These unique advantages make ZnO nanowires a promising matrix electrode for EC dye molecule loading. ZnO nanowires grow vertically from the substrate and form a dense array (Fig. 1). The ZnO nanowires show regular hexagonal cross section and the average diameter of the ZnO nanowires is about 100 nm. The cross-section image of the ZnO nanowires array (Fig. 1) indicates that the length of the ZnO nanowires is about $6\;{\mu}m$. From one on/off cycle of the ZnO EC cell (Fig. 2). We can see that, the switching time of a ZnO nanowire electrode EC cell with an active area of $1\;{\times}\;1\;cm^2$ is 170 ms and 142 ms for coloration and bleaching, respectively. The coloration and bleaching time is faster compared to the $TiO_2$ mesoporous EC devices with both coloration and bleaching time of about 250 ms for a device with an active area of $2.5\;cm^2$. With further optimization, it is possible that the response time can reach ten(s) of millisecond, i.e. capable of displaying video. Fig. 3 shows a prototype with two different transmittance states. It can be seen that good contrast was obtained. The retention was at least a few hours for these prototypes. Being an oxide, ZnO is oxidation resistant, i.e. it is more durable for field emission cathode. ZnO nanotetropods were also applied to realize the first prototype triode field emission device, making use of scattered surface-conduction electrons for field emission (Fig. 4). The device has a high efficiency (field emitted electron to total electron ratio) of about 60%. With this high efficiency, we were able to fabricate some prototype displays (Fig. 5 showing some alphanumerical symbols). ZnO tetrapods have four legs, which guarantees that there is one leg always pointing upward, even using screen printing method to fabricate the cathode.

• Molecules and Cells
• /
• v.43 no.7
• /
• pp.619-631
• /
• 2020

In this study, we describe a novel function of TNNC1 (Troponin C1, Slow Skeletal and Cardiac Type), a component of actin-bound troponin, as a tumor suppressor of lung adenocarcinoma (LUAD). First, the expression of TNNC1 was strongly down-regulated in cancer tissues compared to matched normal lung tissues, and down-regulation of TNNC1 was shown to be strongly correlated with increased mortality among LUAD patients. Interestingly, TNNC1 expression was enhanced by suppression of KRAS, and ectopic expression of TNNC1 in turn inhibited KRAS^G12D-mediated anchorage independent growth of NIH3T3 cells. Consistently, activation of KRAS pathway in LUAD patients was shown to be strongly correlated with down-regulation of TNNC1. In addition, ectopic expression of TNNC1 inhibited colony formation of multiple LUAD cell lines and induced DNA damage, cell cycle arrest and ultimately apoptosis. We further examined potential correlations between expression levels of TNNC1 and various clinical parameters and found that low-level expression is significantly associated with invasiveness of the tumor. Indeed, RNA interference-mediated down-regulation of TNNC1 led to significant enhancement of invasiveness in vitro. Collectively, our data indicate that TNNC1 has a novel function as a tumor suppressor and is targeted for down-regulation by KRAS pathway during the carcinogenesis of LUAD.