• Journal of Acupuncture Research
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• 제27권1호
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• pp.101-107
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• 2010

Objectives : The purpose of this study was to estimate the wrinkle treatment effect of Miso Facial Acupuncture with the facial skin photographing system. Methods : Six middle-aged women with no diseases were recruited. Miso Facial Acupuncture, a total ten times was performed three times a week. The facial skin photograph of each participant was checked two times-pretreatment, posttreatment-using the facial skin photographing system. Both squares and counts of wrinkles in the facial 3 parts were measured for each one. Results : Both squares and counts of wrinkles were decreased on average. The improvement of lower eyelid were the best of all. And the wrinkles of outside of lip were reduced a little. Conclusions : in this study, we could evaluate the wrinkle treatment effects of Miso Facial Acupuncture by an objective and scientific method. Miso Facial Acupuncture has notable effect in wrinkle treatment. As though we had not wide experiences in this treatment, more researches are needed.

• 대한화장품학회지
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• 제35권4호
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• pp.317-323
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• 2009

지속적인 자외선 노출은 사람의 피부에 주름 형성을 유발한다. 본 연구에서 생강나무 추출물이 광노화에 의한 피부 주름 형성의 개선에 미치는 효능을 검증해 보고자 하였다. 우선 사람 섬유아세포를 이용하여 생강나무 추출물의 세포증식과 타입 I 콜라겐의 생합성 활성을 측정하였다. 그 결과 생강나무 추출물에 의한 세포증식과 타입 I 콜라겐의 생합성능은 대조군과 비교하여 각각 33.8 %와 91.8 % 증식함을 보였다. 동물실험에서는 SKH-1 무모쥐에 일주일에 3번 UV를 조사하면서 5 % 생강나무 추출액을 국부적으로 도포하였다. 10주 후에는 각각의 무모쥐의 피부 모사판을 제작하여 관찰하였다. 광노화에 의한 주름형성에 미치는 영향을 알아보고자 UVB를 무모쥐의 피부조직에 조사한 후 생강나무 추출액을 도포하여 피부 상태를 관찰하였다. 모사판을 접사카메라를 이용하여 관찰한 결과 5 % 생강나무 추출액의 도포는 생강나무가 포함되지 않은 도포액을 도포한 대조군에 비해 UV에 의해 생성되는 주름 형성 억제에 영향을 주는 것으로 나타났다. 이러한 결과로 생강나무 추출액의 도포는 광노화에 의한 피부주름 생성을 억제하고 피부를 보호하는 효과가 있을 것으로 판단된다.

• 한국항행학회논문지
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• 제24권2호
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• pp.148-154
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• 2020

주름은 피부의 노화도를 알 수 있는 주요한 특징 중의 하나이다. 기존의 영상처리기반 주름검출은 다양한 피부 영상에 효과적으로 대처하기 어렵다. 특히, 주름이 선명하지 않고 주변 피부와 유사한 경우 주름추출 성능은 급격히 떨어진다. 본 논문에서는 현미경 피부 영상에서 주름추출을 위해 딥러닝을 적용한다. 일반적으로 현미경 영상은 광각렌즈를 탑재하므로 영상 가장자리 영역의 밝기가 어둡다. 본 논문에서는 이를 해결하기 위해 피부 영상의 밝기를 추정하여 보정 한다. 또한, 주름추출에 적합한 의미분할 네트워크의 구조를 적용한다. 제안방법은 연구실에서 수집한 피부 영상에 대한 테스트 실험에서 99.6%의 정확도를 획득하였다.

• 한방안이비인후피부과학회지
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• 제23권1호
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• pp.23-43
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• 2010

Objective : This study was performed to assess the effects of Aloe and Violae herba extracts on skin disease and skin beauty. Methods : Anti-oxidant effects were measured by the scavenging for DPPH radical, xanthine oxidase activity. Anti-inflammatory effects were examined by relations in NO synthesis, IL-$1{\beta}$, IL-6, TNF-$\alpha$, NF-kB, COX-2, MAP kinase. The skin wrinkle formation effects were measured by collagenase and elastase activities. The whitening effects were examined by tyrosinase activities, melanin synthesis in MNT-1 cell. Results : 1. In an anti-oxidant test, Aloe and Violae herba extracts showed high radical scavenging activity. 2. In an anti-inflammatory test, Aloe and Violae herba extracts strongly inhibited the lipopolysaccharide(LPS)-induced nitric oxide(NO) release from the RAW 246.7 macrophage cells. Aloe and Violae herba extracts also inhibited the LPS-induced IL-$1{\beta}$ and COX-2 expressions. The inhibitory effects of Aloe and Violae herba extracts on macrophage activation were via the inhibition of NF-kB, evidenced by transient transfection assay. Furthermore, Aloe and Violae herba extracts weakly inhibited the activation of Jun-N-terminal kinase(JNK) but they did not have any effects on p38 MAP kinase in RAW 264.7 cells. 3. In the skin wrinkle formation assay, Aloe extract strongly inhibited collagenase and elastase, whose activity are tightly related with the wrinkle formation. 4. In the skin whitening assay, Aloe and Viloae herba extracts weakly inhibited tyrosinase activity, however, it was not statistically significant. Besides they did not have any effects on melanin synthesis, indicating that they could not be applicable for skin whitening. Conclusion : This study show that Aloe and Violae herba extracts may play a significant role in skin disease and skin beauty.

• 대한예방한의학회지
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• 제19권1호
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• pp.109-116
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• 2015

Objective : Solar ultraviolet (UV) light can cause photoaging of human skin. Many researchers have focused on effective prevention to mitigate the aging process. This study was aimed to investigate anti-wrinkle effects of pomegranate concentration solution (PCS) using photoaged hairless mouse model. Method : To clarify the protective effect of PCS against UVB-induced photo-damage, water capacity was measured with transepidermal water loss (TEWL) in SKH-1 hairless mice. In addition, image of skin replicas was analyzed. Results : Our results showed that the PCS treatment protects skin against UVB-induced photoaging, based on the parameters including moisture capacity and TEWL. According to visual assessment of skin replica, application of 1 ml/kg PCS inhibited UV-induced wrinkle formation in mice skin as compared to the vehicle-applied control mice. Conclusion : Taken together, these results indicated that PCS could improve skin wrinkle formation induced by UVB irradiation in SHK-1 hairless mice. PCS could be applied as an anti-wrinkle agent.

• Toxicological Research
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• 제35권4호
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• pp.353-359
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• 2019

The purpose of this study was to investigate the effect of $7-MEGA^{TM}$ 500 on the improvement of skin aging in an UVB-induced photo-aging model of hairless mice. The dorsal skin of hairless mice was exposed to UVB three times a week for 12 weeks to induce skin wrinkle. After inducing the wrinkle, $7-MEGA^{TM}$ 500 was orally administered once a day for 4 weeks. Skin thickness, skin barrier function, and wrinkle indicators were improved by treatment with $7-MEGA^{TM}$ 500. Both gene and protein expression levels of MMP-3 and c-Jun in skin were significantly decreased by $7-MEGA^{TM}$ 500. Therefore, the intake of $7-MEGA^{TM}$ 500 is thought to have a positive effect on the improvement of skin aging, although further studies are needed.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book II
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• pp.253-267
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• 2003

Inositol and phytic acic extracted from rice bran were investigated for applying cosmetics. Skin lotions containing 0∼3.0wt% inositol and 0∼1.5wt% phytic acid were applied respectively, to the arm skins of 45 Asian women 20'-40's for 7 weeks. Improvement on moisture was evaluated. In addition, improvements on sebum, elasticity, and wrinkle were examined after applying placebo, Inositol and phytic acid-containing skin lotions for face, respectively. For 1.0 wt% inositol resulted in 19% increase of moisture. The wrinkle reduction and elasticity improved 12.4% and 17.0% on average, respectively. Applying 0.5wt% phytic acid resulted in 71.6% increase the moisture. Improvements on wrinkle and elasticity were 15.9% and 21.9% respectively. Applying inositol or phytic acid regardless of dry or oily, resulted in sebum value recovery to that of the normal skin after 2- 4 weeks. Inositol is inferior to phytic acid in improvenients of the skin, and phytic acid is not suitable to sensitive skin. So 0-0.5wt% of phytic acid were added to 1.0wt% inositol and similar experiments were carried out. In case of added 0.1wt% phytic acid, moisture increased 63.8% approximately. Improvements on elasticity and reduction on wrinkle were 17.2% and 17.4%, respectively. Both skin types were turned to normal skin type after 2 weeks. It could improve the skin condition when used inositol added phytic acid. The optimized concentration of phytic acid was 0.10wt% with 1.0wt% of inositol for synergic effect.

• 한국약용작물학회지
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• 제27권2호
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• pp.143-150
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• 2019

Background: Skin is an organ that protects the human body from various environmental stimuli that can induce immune system activation. Skin aging can be largely divided into two categories: physiological aging, which is caused by the a decreased physiological function of the skin and structural changes with aging, and photoaging, which is caused by the chemical stress induced by external stimuli such as ultraviolet (UV) radiation. Methods and Results: The objective of this study was to investigate the anti-wrinkle and UV protective effect of catechin-rich green tea extract (CGTE) in activated keratinocyte (HaCaT cells) and UV-induced skin damage in hairless mice. The results showed that CGTE inhibits the tumor necrosis factor-alpha interferon-gamma ($TNF-{\alpha}+IFN-{\gamma}$)-induced expression of matrix metalloproteinase (MMP)-1 in HaCaT cells. In addition, the CGTE treatment significantly reduced wrinkle formation, epidermal thickness, collagen deposition, and transepidermal water loss in dorsal skin irradiated with UVB. However, the ${\beta}$-glucosidase activity was significantly increased. The CGTE treatment inhibits mRNA expression and enzyme activity of MMP-2 and MMP-9 in the dorsal skin irradiated with UVB. Conclusions: It is expected that CGTE can be effectively used as a functional food and cosmetic ingredient to improve skin moisture retention and reduce wrinkle formation.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book I
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• pp.617-629
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• 2003

A recent development in cosmetics has been the pursuit of wrinkling in the skin. The cosmetics composed of anti-wrinkle agent stand out from the point of view of environmental contamination and pollution. Among them, Indole-3-acetic acid (IAA), studied with wrinkling pigmentation and swelling conditions in the area of the eye, showed clinically significant reduction in depth of lines during one month trial using skin treatment. But, IAA has shown some problems when used in cosmetic formulations, such as stability, permeability and toxicity. The results of the clinical examination were shown that its permeability and toxicity didn't matter. To increase the stability of IAA, antioxidants such as Licorice, ubiquinone, tocopherol, Baicalin, ferulic acid, BHT, ascorbic acid, sodium metabisulfite, and so on were employed in cosmetic formulations. Our main purpose is the study for the stability efficiency and effect of each other of cream formulations containing optimal dosage antioxidants (o/w type emulsion), This study evaluated wrinkle reduction effect of IAA, which is used in cosmetics.

• 대한본초학회지
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• 제32권5호
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• pp.7-12
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• 2017

Objectives : Recently, numerous studies reported that excessive generation of advanced glycation end products (AGEs) stimulated expression of skin wrinkle related proteins. This study aimed to evaluate inhibits skin wrinkle formation effect of Mori Folium (MF) through decreased AGEs. Methods : To evaluate the skin wrinkle inhibition effect of MF, SD-rats were divided into three groups; normal rats (Nor), AGEs-induced rats (Con), AGEs-induce rats treated with MF at dose of 100mg/kg body weight (MF). To induced AGEs, streptozotocin (50mg/kg) was injected intraperitoneally, and after 3 days, 100mM methyl glyoxal was administered orally for 3 weeks. After the experiment, the animal's dorsal skin tissues and serum were separated and tested. Results : The oral administration of MF suppressed the AGEs level in serum. Also, the AGEs in skin tissues was significantly reduced through treatment of MF compared with control group. Moreover, the expressions of AGEs related proteins such as polyclonal anti-$N^e$-(carboxymethyl) lysine (CML), anti-$N^e$-(carboxyethyl)lysine (CEL), AGE receptors (RAGE) were reduced in MF group compared with the control group in kidney and skin tissues. The matrix metallo proteinase-1 (MMP-1) reduced by MF treatment with the result that collagen type 1 alpha 2 (COL1A2) was improved that reduced by accumulation of AGEs. Conclusion : The evidence of this study indicate that oral administration of MF reduces the levels of AGEs in serum, skin, and kidney tissues. In conclusion, MF inhibit skin wrinkle formation, suggesting the potential of anti-wrinkle material.