• Title/Summary/Keyword: Skeletal growth

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IGF-I Exerts an Anti-inflammatory Effect on Skeletal Muscle Cells through Down-regulation of TLR4 Signaling

  • Lee, Won-Jun
    • IMMUNE NETWORK
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    • v.11 no.4
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    • pp.223-226
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    • 2011
  • Although exercise-induced growth factors such as Insulin-like growth factor-I (IGF-I) are known to affect various aspects of physiology in skeletal muscle cells, the molecular mechanism by which IGF-I modulates anti-inflammatory effects in these cells is presently unknown. Here, we showed that IGF-I stimulation suppresses the expression of toll-like receptor 4 (TLR4), a key innate immune receptor. A pharmacological inhibitor study further showed that PI3K/Akt signaling pathway is required for IGF-I-mediated negative regulation of TLR4 expression. Furthermore, IGF-I treatment reduced the expression of various NF-${\kappa}B$-target genes such as TNF-${\alpha}$ and IL-6. Taken together, these findings indicate that the anti-inflammatory effect of exercise may be due, at least in part, to IGF-I-induced suppression of TLR4 and subsequent downregulation of the TLR4-dependent inflammatory signaling pathway.

Successful treatment of growing skeletal class II children with Twin-Block appliance. (트윈블록을 이용한 성장기 II급 아동의 성공적인 하악골 성장 치료)

  • Kim, Seong Sik;Kim, Sung Hun;Kim, Yongil;Park, Soobyung;Son, Woosung
    • The Journal of the Korean dental association
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    • v.56 no.2
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    • pp.103-112
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    • 2018
  • The treatment of skeletal Class II growing patient is to move the mandible into the Class I molar positon via facilitating mandibular growth. The functional appliances are to be designed to exert three major functions such as palatal expansion, forward growth of mandible and increase of the posterior vertical dimension. One of the devices that can achieve both the palatal expansion and the eruption of the mandibular molar is the Twin-Block introduced by Clark in 1982. In this part, we present the treatment method with Twin-Block functional appliance for the correction of skeletal Class II growing patient.

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The growth stage study on the femur and tibia of Korean native chicken after hatching (한국 재래닭의 부화 후 성장단계별 넙다리뼈와 정강뼈 성장에 관한 연구)

  • Tae, Hyun-Jin
    • Korean Journal of Veterinary Service
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    • v.38 no.3
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    • pp.181-188
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    • 2015
  • Skeletal development of chicken has been widely discussed in industrial forums and various research reports. However, these studies were emphasis on the commercial chicken strains for improve egg and meat production whereas the skeletal quiet remains as a potential weak link related to facilitating in the physical support of heavier carcasses at ever younger ages. For that, the study of standardization of skeletal development is important but it was rarely reported in Korean native chicken (KNC). The study was investigated the skeletal characteristics of KNC for international standardization. We studied in KNC at 2, 14, 28, 42, 56, 70, 84, 98, 112, 126, 147, 168, 196, 224, 336 and 448 days after hatch (male and female, n=13 for each group). We measured the body weight (BW), and after sacrifice measured organs and remove muscle from femur & tibia and measured bone weight. Data were analyzed by ANOVA, Duncan test, correlation analysis and regression analysis of SAS 9.1. We analyzed the data of BW, femur & tibia and made growth curve also. The BW was significantly increased up to 147 days after hatch (male, $1,927.88{\pm}68.92g$; female, $1,456.00{\pm}50.11g$), and then increased gradually. At 336 days, these growth was stop (male, $2,467.00{\pm}42.84g$; female, $1,568.71{\pm}62.62g$). The growth of femur & tibia length and width was stop on 98~126 days after hatch. At 98 days, we measured the length and width of femur & tibia in male were $132.39{\pm}3.18mm$ & $25.98{\pm}0.59mm$ whereas in female at 112 days the length of femur & tibia was $116.40{\pm}1.55mm$ and at 126 days width was $21.41{\pm}0.38mm$. Our study suggests that the growth of male KNC was classified pre-puberty (0~98 days), puberty (98~336 days) and maturity (after 336 days), meanwhile female was shown similar trend however puberty period of KNC was 112 or 126 days after hatch.

Evaluation of cephalometric characteristics and skeletal maturation of the cervical vertebrae and hand-wrist in girls with central precocious puberty

  • Kang, Sung-Tae;Choi, Sung-Hwan;Kim, Kyung-Ho;Hwang, Chung-Ju
    • The korean journal of orthodontics
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    • v.50 no.3
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    • pp.181-187
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    • 2020
  • Objective: This study aimed to evaluate the differences in cephalometric characteristics and skeletal maturation in girls with central precocious puberty (CPP) via lateral and hand-wrist radiographs. We also aimed to identify the indicators that are most effective for determining skeletal maturity in these patients. Methods: The study included 70 Korean girls (mean age, 8.5 ± 0.5 years) diagnosed with CPP at the Department of Pediatrics, and 48 normal healthy age-matched girls who visited the Department of Orthodontics and had no history of hormone treatment or growth problems. Skeletal maturation was evaluated using lateral cephalometric and hand-wrist radiographs using cervical vertebrae maturation indicators (CVMI) and skeletal maturity indicators (SMI). Results: The mean mandibular plane angle was smaller in the CPP group than in the control group (35.8° ± 4.9° vs. 39.0° ± 6.5°), resulting in greater posterior facial height (p = 0.003). SMI was significantly greater in the CPP group (3.5 ± 1.4 vs. 2.0 ± 1.0) than in the control group (p = 0.001) and was significantly associated with CPP (r = 0.492; p = 0.001), whereas CVMI was not. Conclusions: In comparison with the control group, the CPP group exhibited a smaller mandibular plane angle, greater posterior facial height, and greater skeletal maturation. SMI may be more suitable than CVMI for determining skeletal maturation in CPP. Hand-wrist radiography is recommended in addition to lateral cephalogram for predicting growth in girls with CPP.

TREATMENT EFFECT OF PROTRACTION HEAD GEAR ON SKELETAL CLASS III III MALOCCLUSION (골격성 제III급 부정교합 환자에 대한 상악골 전방견인 장치의 치료효과)

  • Hwang, Chung-Ju;Kyung, Seung-Hyun;Lim, Joong-Ki
    • The korean journal of orthodontics
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    • v.24 no.4 s.47
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    • pp.851-860
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    • 1994
  • Before 1970, mandibular overgrowth was known as main cause of skeletal Class III malocclusion in growing children ; however, recent study reports that many skeletal Class III malocclusion patients also show maxillary deficiency. Since 1972, when Delaire re-accommodated Protraction Head Gear (P.H.G.), many researchers have reported that skeletal Class III discrepancies could be corrected through use of P.H.G., which induces anterior movement of maxilla and change in mandibular growth pattern into infero-posterior direction ; nevertheless, it is very difficult to predict resultant changes of orofacial region. The purpose of this study was to find out what treatment effect P.H.G. has on different study samples. Author divided 51 skeletal Class III malocclusion patients with maxillary deficiency who were treated with P.H.G. into different study groups depending on sex, treatment beginning age, intraoral appliance, and facial growth pattern. By doing so, following results were obtained. 1. Treatment beginning age and Sex Four age groups (5.8 to 8 year-old, 8 to 10 year-old, 10 to 12 year-old, 12 to 14 year-old) were compared, and no significant difference was observed. (p<0.05) There was no significant difference between the sex groups, either. (p<0.05). 2. Intraoral appliance Treatment effects of study groups that used R.P.E.(mean age of 10.2) and Labio-Lingual appliance(mean age of 8.9) were compared. There was no significant difference depending on the type of intraoral appliance that was used. (p<0.05) 3. Facial growth pattern 1) Amounts of SNB and ANB corrections were smaller in clockwise growth pattern group than those in normal or counterclockwise growth pattern group. (p<0.05) 2) Amounts of increase in Wits appraisal and mandibular plane angle were greater in counterclockwise growth pattern group than those in normal or clockwise growth pattern group. (p<0.05) 3)Amounts of increase in articular angle were greater in counter lockwise growth pattern group than those in clockwise growth pattern group. (p<0.05)

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Changes in expression of the autophagy-related genes microtubule-associated protein 1 light chain 3β and autophagy related 7 in skeletal muscle of fattening Japanese Black cattle: a pilot study

  • Nakanishi, Tomonori;Tokunaga, Tadaaki;Ishida, Takafumi;Kobayashi, Ikuo;Katahama, Yuta;Yano, Azusa;Erickson, Laurie;Kawahara, Satoshi
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.4
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    • pp.592-598
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    • 2019
  • Objective: Autophagy is a bulk degradation system for intracellular proteins which contributes to skeletal muscle homeostasis, according to previous studies in humans and rodents. However, there is a lack of information on the physiological role of autophagy in the skeletal muscle of meat animals. This study was planned as a pilot study to investigate changes in expression of two major autophagy-related genes, microtubule-associated protein 1 light chain $3{\beta}$ (MAP1LC3B) and autophagy related 7 (ATG7) in fattening beef cattle, and to compare them with skeletal muscle growth. Methods: Six castrated Japanese Black cattle (initial body weight: $503{\pm}20kg$) were enrolled in this study and fattened for 7 months. Three skeletal muscles, M. longissimus, M. gluteus medius, and M. semimembranosus, were collected by needle biopsy three times during the observation period, and mRNA levels of MAP1LC3B and ATG7 were determined by quantitative reverse-transcription polymerase chain reaction. The expression levels of genes associated with the ubiquitin-proteasome system, another proteolytic mechanism, were also analyzed for comparison with autophagy-related genes. In addition, ultrasonic scanning was repeatedly performed to measure M. longissimus area as an index of muscle growth. Results: Our results showed that both MAP1LC3B and ATG7 expression increased over the observation period in all three skeletal muscles. Interestingly, the increase in expression of these two genes in M. longissimus was highly correlated with ultrasonic M. longissimus area and body weight. On the other hand, the expression of genes associated with the ubiquitin-proteasome system was unchanged during the same period. Conclusion: These findings suggest that autophagy plays an important role in the growth of skeletal muscle of fattening beef cattle and imply that autophagic activity affects meat productivity.

TREATMENT OF SKELETAL CLASS II MALOCCLUSION BY COMBINATION THERAPY OF ACTIVATOR WITH ANTERIOR HIGH PULL HEADGEAR (Activator와 Anterior high pull headgear를 이용한 골격성 II급 부정교합의 치험례)

  • Yang, Kyu-Ho;Kim, Jung-Ran;Choi, Nam-Gi
    • Journal of the korean academy of Pediatric Dentistry
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    • v.26 no.1
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    • pp.126-132
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    • 1999
  • This article describes the use of an activator with anterior high pull headgear to treat a skeletal Class II malocclusion in children in the mixed-dentition phase. A combination of headgear-activator appliance can inhibit forward and downward growth of the maxillary complex while stimulating mandibular growth. The correction of Class II malocclusion can be achieved by careful case selection of a motivated patient with a favorable growth pattern. The patients who have skeletal Class II malocclusions were treated by means of activator with anterior high pull headgear and the following results were observed; 1. Forward and downward growth of the maxillary complex were inhibited 2. Mandibular growth was stimulated and counterclockwise rotation of the mandible was observed. 3. Large overjet and deep overbite were corrected.

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A LONGITUDINAL STUDY ON THE PUBERTAL GROWTH PEAK AND MATURITY STAGES OF THE HAND-WRIST IN MALOCCLUSION (부정교합자(不正咬合者)의 사춘기성장(思春期成長)과 수완(手腕) 부골(部骨) 성숙단계(成熟段階)에 관(關)한 누년적(累年的) 연구(硏究))

  • Kim, Hyung-Il;Lee, Dong- Joo
    • The korean journal of orthodontics
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    • v.19 no.3
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    • pp.123-133
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    • 1989
  • To predict the pubertal growth peak in stature and study the skeletal maturity degree using hand-wrist radiograph, the author used the 70 malocclusions (male 24, female 46). After longitudinal measurement of stature and skeletal maturity indicators of hand-wrist radiographs were taken during 4 years, the rsults were as follows. 1) The pubertal growth peak in stature occurred mainly at SMI 6-7 (56.5%) in female, SMI 5-6 (37.5%), 6-7 (37.5%) in male (Table 5). 2) It was suggested that the pubertal growth peak in stature was already passed, if SMI 8 occurred. 3) Ages of SMI in female were about 2 years earlier than those of SMI in male, and the sexual difference was gradually decreased in puberty. 4) Duration of SMI was longest at SMI 6-7 in both sex and the mean was 8.5 months. After this stage, the velocity of skeletal maturity in female was decreased than in male. 5) The correlation coefficient between each SMI and pubertal growth peak was very high (Table 8).

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Insulin-Like Growth Factor-I Induces Androgen Receptor Activation in Differentiating C2C12 Skeletal Muscle Cells

  • Kim, Hye Jin;Lee, Won Jun
    • Molecules and Cells
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    • v.28 no.3
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    • pp.189-194
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    • 2009
  • The modulating effect of IGF-I on the regulation of AR gene expression and activation in skeletal muscle cells remains poorly understood. In this study, the effects of IGF-I treatment on AR induction and activation in the absence of AR ligands were examined. Differentiating C2C12 cells were treated with different concentrations (0-250 ng/ml) of IGF-I or for various periods of time (0-60 min) of 250 ng/ml IGF-I. Treatment of C2C12 cells with IGF-I resulted in a dose- and time-dependent increase in total AR and phosphorylated AR (Ser 213). IGF-I treatment also led to significantly increased AR mRNA expression when compared with the control. The levels of skeletal ${\alpha}-actin$ and myogenin mRNA, known target genes of AR, were also significantly upregulated after 5 or 10 min of treatment with IGF-I. Confocal images revealed that IGF-I stimulated nuclear localization of AR in the absence of ligands. In addition, an electrophoretic mobility shift assay indicated that IGF-I stimulated the AR DNA binding activity in a time-dependent manner. The present results suggest that IGF-I stimulates the expression and activation of AR by ligand-independent mechanism in differentiating C2C12 mouse skeletal muscle cells.

The Effect of Ginseng on the Calcium Uptake by Sarcoplasmic Reticulum Fragments Isolated from Potassium Deficient Rat Skeletal Muscles (인삼이 칼슘결핍 흰쥐골격근의 소포체에 의한 칼슘 - Uptake에 미치는 영향)

  • 김낙균;김낙두;이종욱
    • YAKHAK HOEJI
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    • v.30 no.3
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    • pp.143-148
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    • 1986
  • The effect of orally administered ginseng ethanol extract on potassium deficient and normal rat skeletal muscles was investigated in terms of Ca uptake by sarcoplastic reticulum fragments. The ginseng ethanol extract (100mg/kg/day) was administered orally to Sprague-Dawley rats for 21 days and their changes of body weights, $K^+$ content in skeletal muscles, and calcium uptake capacity of sarcoplamic reticulurn of each groups were measured. The growth rate of rats fed with the potassium deficient diet was significantly decreased compared to that of normal rats. Ginseng components did not show any effect on the decreased growth rate of the postassium deficient rats. Potassium content in skeletal muscle from potassium deficient rats was significantly reduced compared to that of normal rats. Ginseng components showed the tendency to prevent the reduction in potassium content of potassium deficient rats, but differences were not statistically significant. Calcium uptake of SR prepared from skeletal muscles of potassium deficient rats was increased significantly compared to that of normal rats. Ginseng components prevented such increase of calcium uptake by 30%. In summary, it can be concluded that ginseng may prevent the increase in Cauptake of SR obtained from potassium deficient rats.

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