• Tuberculosis and Respiratory Diseases
• /
• 제73권3호
• /
• pp.143-150
• /
• 2012

Background: The release of interferon-gamma (IFN-${\gamma}$) by T lymphocytes increases after rechallenge with Mycobacterium tuberculosis antigen, especially, at a localized site of tuberculosis (TB) infection. We aimed to compare the clincial efficacy of two commercial IFN-${\gamma}$ release assays from pleural fluid for the diagnosis in tuberculous pleurisy. Methods: We performed T-SPOT.TB and QuantiFERON-TB Gold tests simultaneously on pleural fluid and peripheral blood samples from patients with pleural effusion, in South Korea, an area with intermediate TB burden. Results: Thirty-six patients were enrolled prospectively, and tuberculous pleurisy was found in 21 patients. Both the numbers of IFN-${\gamma}$ secreting T cells and the concentration of IFN-${\gamma}$ were greater in the pleural tuberculous group, comparing with the non-tuberculous group. Moreover, in the tuberculous group, there was a significant difference in IFN-${\gamma}$ producing spot-forming cells using the T-SPOT.TB method between pleural fluid and peripheral blood. The receiver operating characteristic (ROC) curve, was the greatest for pleural fluid T-SPOT.TB test, followed by peripheral blood T-SPOT.TB test, peripheral blood QuantiFERON-TB Gold test, and pleural fluid QuantiFERON-TB Gold test (area under the ROC curve of 0.956, 0.890, 0.743, and 0.721, respectively). The T-SPOT.TB assay produced less indeterminate results than did QuantiFERON-TB Gold assay in both pleural fluid and peripheral blood. Conclusion: These findings suggest that the pleural fluid T-SPOT.TB test could be the most useful test among the IFN-${\gamma}$ release assays for diagnosing tuberculous pleurisy in an area with an intermediate prevalence of TB infection.

• 공업화학
• /
• 제29권2호
• /
• pp.147-154
• /
• 2018

현재 항암제의 낮은 치료 효율과 부작용을 해결하기 위해 고분자 기반의 약물전달체의 연구가 활발하게 진행되고 있다. 기존의 고분자기반의 약물 전달체는 우수한 결과를 보이는 등 상당한 진전이 있었음에도 불구하고, 대부분 혈중에서 안정성이 감소하여 표적 부위에 도달하기 전에 약물이 방출될 뿐만 아니라 오랜 시간 동안에 약물을 방출함으로써 부작용 및 낮은 치료 효율을 초래한다는 문제점을 가지고 있다. 본 총론에서는 이러한 비효율적인 약물 방출의 문제점을 개선하기 위한 방법으로 독성이 없고 생체 적합한 천연 고분자 키토산에 자극 응답성 물질을 도입하여 혈중에서 안정성을 높이고 표적 부위에서 약물을 과다 방출하여 치료 효율을 극대화할 수 있는 방법을 제시하고자 한다.

• 환경생물
• /
• 제32권4호
• /
• pp.327-334
• /
• 2014

Emission reduction of $CH_4$ (methane gas) from rice paddy soil is a very important measure for climate change mitigation in agricultural sector. In this study, we investigated the changes in crop yield and $CH_4$ emissions in response to application of biochar and fertilizers. The experimental site is located in Hwasung, Kyunggido and experimental design is the split-plot method with three replicates. Treatments included rice straw (RS) and biochar (BC) amendments nested with the conventional NPK fertilizer (NPK) and slow release fertilizer (SRF). Control was also prepared with the soil with the conventional NPK fertilization with no amendment. Measurement of $CH_4$ emission was conducted during the growing season of 2014 using a dynamic chamber method. The results showed that application of rice straw increased daily $CH_4$ emission rate by 15%, while application of biochar reduced daily $CH_4$ emission rate by 38%. When we combined biochar application with slow release fertilizer, $CH_4$ emission was reduced by 45%. Further, the crop yield was also increased in all treatments compared with the control except for the treatment of rice straw application with slow release fertilizer. Overall results imply that biochar amendment to agricultural soil can be an effective strategy to decrease annual $CH_4$ emission with no reduction in crop yield.

• 한국농림기상학회지
• /
• 제6권3호
• /
• pp.177-182
• /
• 2004

기온의 공간변이가 심한 넓은 지역에서 Chill Unit 축적량과 휴면해제일의 공간분포를 얻기 위해서 Utah Chill Unit 모형을 지리정보시스템과 결합하여 Chill Unit 공간적산기(CUMAP)을 개발하였다. CUMAP은 Grid 형태의 일 최고, 최저기온을 입력받아 시간내삽법에 의해 매시기온을 추정하고, Utah 모형에 의해 매시 Chill Unit을 계산한다. 하루 단위로 Chill Unit이 누적되면 Grid파일로 저장되며, 사용자가 정하는 저온요구량(임계값)에 도달하는 날짜를 역시 Grid파일로 생성한다. 전라북도 김제시 백구면 일대 포도재배지역에 대해 CUMAP을 적용하여 10m 해상도의 Chill Unit 일별 적산값과 휴면해제일 추정분포도를 작성하였다.

• Nuclear Engineering and Technology
• /
• 제42권1호
• /
• pp.17-36
• /
• 2010

In this study, we compare the mass release rates of radionuclides(1) from waste forms arising from the KIEP-21 pyroprocessing system with (2) those from the directly-disposed pressurized-water reactor spent fuel, to investigate the potential radiological and environmental impacts. In both cases, most actinides and their daughters have been observed to remain in the vicinity of waste packages as precipitates because of their low solubility. The effects of the waste-form alteration rate on the release of radionuclides from the engineered-barrier boundary have been found to be significant, especially for congruently released radionuclides. the total mass release rate of radionuclides from direct disposal concept is similar to those from the pyroprocessing disposal concept. While the mass release rates for most radionuclides would decrease to negligible levels due to radioactive decay while in the engineered barriers and the surrounding host rock in both cases even without assuming any dilution or dispersal mechanisms during their transport, significant mass release rates for three fission-product radionuclides, $^{129}I$, $^{79}Se$, and $^{36}Cl$, are observed at the 1,000-m location in the host rock. For these three radionuclides, we need to account for dilution/dispersal in the geosphere and the biosphere to confirm finally that the repository would achieve sufficient level of radiological safety. This can be done only after we have known where the repository site would by sited. the footprint of repository for the KIEP-21 system is about one tenth of those for the direct disposal.

• Journal of Veterinary Science
• /
• 제25권2호
• /
• pp.30.1-30.16
• /
• 2024

Background: Biofilms, such as those from Staphylococcus epidermidis, are generally insensitive to traditional antimicrobial agents, making it difficult to inhibit their formation. Although quercetin has excellent antibiofilm effects, its clinical applications are limited by the lack of sustained and targeted release at the site of S. epidermidis infection. Objectives: Polyethylene glycol-quercetin nanoparticles (PQ-NPs)-loaded gelatin-N,O-carboxymethyl chitosan (N,O-CMCS) composite nanogels were prepared and assessed for the on-demand release potential for reducing S. epidermidis biofilm formation. Methods: The formation mechanism, physicochemical characterization, and antibiofilm activity of PQ-nanogels against S. epidermidis were studied. Results: Physicochemical characterization confirmed that PQ-nanogels had been prepared by the electrostatic interactions between gelatin and N,O-CMCS with sodium tripolyphosphate. The PQ-nanogels exhibited obvious pH and gelatinase-responsive to achieve on-demand release in the micro-environment (pH 5.5 and gelatinase) of S. epidermidis. In addition, PQ-nanogels had excellent antibiofilm activity, and the potential antibiofilm mechanism may enhance its antibiofilm activity by reducing its relative biofilm formation, surface hydrophobicity, exopolysaccharides production, and eDNA production. Conclusions: This study will guide the development of the dual responsiveness (pH and gelatinase) of nanogels to achieve on-demand release for reducing S. epidermidis biofilm formation.

• 식물조직배양학회지
• /
• 제27권5호
• /
• pp.375-377
• /
• 2000

It is now generally accepted that a phosphoinositide cycle is involved in the transduction of a variety of signals in plant cells. In animal cells, the hydrolysis of phosphatidyl-4,5-bisphosphate catalysed by phosphatidylinositol - specific phospholipase C yields to D-myo-inositol - 1,4,5-trisphosphate and diacylglycerol, which are well known second messengers. The binding of InsP$_3$to a receptor located on the endoplasmic reticulum triggers a calcium release from the endoplasmic reticulum. We have detected and partially characterised key components of phosphoinositide signaling. First, tobacco microsomal fraction and plasma membrane PI-PLC. Consecutively, using a radioligand binding assay we have identified a $Ca^{2+}$ -dependent high affinity InsP$_3$binding site in microsomal membrane fraction vesicle preparation and then we have measured inositol-1,4,5-trisphosphate induced calcium release from tobacco microsomal fraction. These findings suggest that phosphoinositide signaling system is present and operates in the tobacco suspension culture.e.

• Journal of Pharmaceutical Investigation
• /
• 제22권1호
• /
• pp.1-10
• /
• 1992

The use of erythrocyte as drug carrier has been reviewed, Carrier erythrocytes have proven to offer many advantages for delivery of therapeutic agents, especially in the treatment of inherited enzyme deficiency and cancer. Carrier erythrocytes are biodegradable and nonimmunogenic. Encapsulated drugs may be protected from premature degradation, inactivation and excretion. Carrier erythrocytes may be used as a slow-release system. Targeting of encapsulated drugs directly to a site of action is another possibility. Methods for encapsulating drugs into erythrocytes, the fate of carrier erythrocytes in vivo, the strategies of targeting carrier erythrocytes to special organs and in vivo applications of erythrocytes have been discussed. The encapsulation of drugs in erythrocytes has shown attractive possibilites in future use.

• 대한안전경영과학회:학술대회논문집
• /
• 대한안전경영과학회 2006년도 추계공동학술대회
• /
• pp.499-507
• /
• 2006

The tower cranes are widely used in very useful construction machine the sites of constructing high-structure and have a structural sensitiveness. Therefore, the accidents have often happened due to the deficiency of laborer's understanding md lack of safety of structure. Till now, as we have research and studied above, we can properly protect accidents by construction equipments particularly crane as well as most disasters which occur frequently in construction site. The goal of this study is the safety inspection model of the tower crane a construction site, which preventible the collapse accident of tower crane which is constructed by using the correcting frame. In order to accomplish the goal of this study, the field survey, the reference investigation and the structure analysis were performed for the collapse accident of tower nine using the correcting data. This study will be proposed a build-up solutions about operating and release of safety constructions and researched about software safety estimation. Also, preventing safety problems of Tower Crane Construction site as applying safety estimation program and laws and regulations. As a result, The real time control of tower crane inspection system is implemented by to illustrate the application of the adopted optimal design model.

• 대한안전경영과학회지
• /
• 제9권1호
• /
• pp.37-49
• /
• 2007

The tower cranes are widely used in very useful construction machine the sites of constructing high-structure and have a structural sensitiveness. Therefore, the accidents have often happened due to the deficiency of laborer's understanding and lack of safety of structure. Till now, as we have research and studied above, we can properly protect accidents by construction equipments particularly crane as well as most disasters which occur frequently in construction site. The goal of this study is the safety inspection model of the tower crane a construction site, which preventible the collapse accident of tower crane which is constructed by using the correcting frame. In order to accomplish the goal of this study, the field survey, the reference investigation and the structure analysis were performed for the collapse accident of tower crane using the correcting data. This study will be proposed a build-up solutions about operating and release of safety constructions and researched about software safety estimation. Also, preventing safety problems of Tower Crane Construction site as applying safety estimation program and laws and regulations. As a result, The real time control of tower crane inspection system is implemented by to illustrate the application of the adopted optimal design model.