• Korean Journal of Microbiology
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• v.46 no.4
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• pp.366-374
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• 2010

Culture-dependent RFLP and culture-independent DGGE were employed to investigate the bacterial community associated with the marine sponge Spirastrella abata. A total of 164 bacterial strains associated with the sponge were cultivated using Zobell and Natural sea salt media. PCR amplicons of the 16S rDNA from the bacterial strains were digested with the restriction enzymes HaeIII and MspI, and then assigned into different groups according to their restriction patterns. The 16S rDNA sequences derived from RFLP patterns showed more than 95% similarities compared with known bacterial species, and the isolates belonged to four phyla, Proteobacteria (Alphaproteobacteria, Gammaproteobacteria), Actinobacteria, Firmicutes, and Bacteriodetes, of which Alphaproteobacteria was dominant. DGGE fingerprinting of 16S rDNAs amplified from the sponge- derived total gDNA showed five major DGGE bands, and their sequences showed more than 96% similarities compared with available sequences. The sequences derived from DGGE bands revealed high similarity with the uncultured bacterial clones. DGGE revealed that bacterial community consisted of four phyla, including Proteobacteria (Alphaproteobacteria, Gammaproteobacteria), Actinobacteria, Spirochetes, and Chloroflexi. Alphaproteobacteria, Gammaproteobacteria, and Actinobacteria were commonly found in bacteria associated with S. abata by both RFLP and DGGE methods; however, overall bacterial community in the sponge differed depending on the analysis methods.

• The KIPS Transactions:PartD
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• v.18D no.6
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• pp.501-508
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• 2011

A knowledge map, which has been recently applied in various fields, is discovering characteristics hidden in a large amount of information and showing a tangible output to understand the meaning of the discovery. In this paper, we suggested a knowledge map for research trend analysis based on keyword-relation networks which are constructed by using a database of the domestic journal articles in the computer engineering field from 2000 through 2010. From that knowledge map, we could infer influential changes of a research topic related a specific keyword through examining the change of sizes of the connected components to which the keyword belongs in the keyword-relation networks. In addition, we observed that the size of the largest connected component in the keyword-relation networks is relatively small and groups of high-similarity keyword pairs are clustered in them by comparison with the random networks. This implies that the research field corresponding to the largest connected component is not so huge and many small-scale topics included in it are highly clustered and loosely-connected to each other. our proposed knowledge map can be considered as a approach for the research trend analysis while it is impossible to obtain those results by conventional approaches such as analyzing the frequency of an individual keyword.

• Journal of the Institute of Convergence Signal Processing
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• v.12 no.4
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• pp.258-266
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• 2011

AR(Augmented Reality) technology is now easily shown around us with respect to its applicable areas' being spreaded into various shapes since the usage is simply generalized and many-sided. Currently existing camera vision based AR used marker based methods rather than using real world's informations. For the marker based AR technology, there are limitations on applicable areas and its environmental properties that a user could immerse into the usage of application program. In this paper, we proposed a novel AR method which users could recognize objects from the real world's data and the related 3-dimensional contents are also displayed. Those are done using image processing skills and a smart mobile embedded camera for terminal based AR implementations without any markers. Object recognition is done from the comparison of pre-registered and referenced images. In this process, we tried to minimize the amount of computations of similarity measurements for improving working speed by considering features of smart mobile devices. Additionally, the proposed method is designed to perform reciprocal interactions through touch events using smart mobile devices after the 3-dimensional contents are displayed on the screen. Since then, a user is able to acquire object related informations through a web browser with respect to the user's choice. With the system described in this paper, we analyzed and compared a degree of object recognition, working speed, recognition error for functional differences to the existing AR technologies. The experimental results are presented and verified in smart mobile environments to be considered as an alternate and appropriate AR technology.

• The Journal of Korean Society of Virology
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• v.28 no.3
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• pp.275-285
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• 1998

Based on the geographic range and distribution of its rodent reservoir host, the European common vole (Microtus arvalis), Tula virus is likely to be widespread throughout Eurasia. Tula virus-infected voles have been captured in Central Russia, Austria, Czech and Slovak Republics, and the former Yugoslavia. Although serologic evidence for Hantaan (HTN) or Seoul (SEO) virus infection can be found in the vast majority of the more than 300 cases of hemorrhagic fever with renal syndrome (HFRS) occurring annually in Korea, approximately 4% of Korean patients with HFRS show a more than 4-fold higher antibody titer to Puumala (PUU) virus than to HTN or SEO virus by double-sandwich IgM ELISA, suggesting the existence of pathogenic Puumala-related hantaviruses in Korea. To further define the geographic distribution and genetic diversity of Tula virus in Eurasia and to investigate the existence of previously unrecognized Microtus-borne hantavirus in Korea, arvicolid rodents were captured in Lodz, Poland in 1995 and in Yunchon-kun, Kyungki-do during April to May, 1998. In addition, sera from 18 Korean HFRS patients who showed higher (or the same) antibody titer to Tula virus than HTN and SEO viruses were examined for hantavirus RNA by RT-PCR. Hantaviral sequences were not detected in any of the 18 patients or in 35 reed voles (Microtus fortis) in Korea. Alignment and comparison of a 208-nucleotide region of the S segment, amplified from lung tissues of two hantavirus-seropositive Marvalis captured in Poland, revealed $80.8{\sim}83.2%$ sequence similarity, respectively, with Tula virus strains from Central Russia and the Czech and Slovak Republics. Phylogenetic analysis indicated that the newfound Tula virus strains from Poland were closely related to other Tula hantaviruses from Eurasia.

• Journal of Life Science
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• v.14 no.1
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• pp.21-25
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• 2004

To identify genes involved in the decolorization of both crystal violet and malachite green, we isolated random mutants generated by transposon insertion in triphenylmethane-decolorizing bacterium, Citrobacter sp. The resulting mutant bank yielded 14 mutants with complete defect in color removal capability of both crystal violet and malachite green. Southern hybridization with a Tn5 fragment as a probe showed a single hybridized band in 5 mutants and these mutants appeared to have insertions at different sites of the chromosome. Tn5-inserted genes were isolated and the DNA sequence flanking Tn5 was determined. From comparison with a sequence database, putative protein products encoded by cmg genes were identified as follows. cmg 2 is MaIC protein in maltose transport system; cmg 6 is transcriptional regulator (LysR-type): cmg 12 is a putative oxidoreductase. The sequences deduced from two cmg genes, cmg 8 and cmg 11, showed no significant similarity to any protein with a known function. Therefore, these results indicate that these two cmg genes encode unidentified proteins responsible for decolorization of both crystal violet and malachite green.

• Journal of Korea Water Resources Association
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• v.41 no.2
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• pp.212-228
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• 2008

Five representative reference evapotranspiration(RET) equations were selected, and these equations were compared with pan evaporation by correlation analysis. Pan coefficients were also estimated. Furthermore, five selected RET equations were compared to find the similarity among those at the 21 meteorological stations located in South Korea. Five RET equations selected from 4 different category were Penman(combination approach), FAO Penman-Monteith(FAO P-M) (single source approach), Makkink and Priestley-Taylor (radiation approach) and Hargreaves(temperature approach) equations. In this study, the geographical and topographical conditions were considered for the selection of study stations. The daily meteorological data measured from 1970 at an interval of 5 years were applied in this study. The evapotranspiration estimates obtained by applying evapotranspiration equations were evaluated with numerical and graphical methods. The correlation coefficients between pan evaporation and RET in study stations were above 0.9 indicating very high correlation; however, the slopes of the individual regression lines show the values greater or less than 1.0. Hargreaves equation(temperature approach) shows the most similar evapotranspiration estimates to those of FAO P-M equation from 12 study stations, which are located near to seashore except Daegu station. On the other hand, Priestley-Taylor equation(radiation approach) shows the most similar evapotranspiration estimates to those of FAO P-M equation from 8 study stations, which are located in inland.

• Ecology and Resilient Infrastructure
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• v.2 no.1
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• pp.80-92
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• 2015

Some of alien plants, which were introduced from foreign countries, have caused problems in Korea. Invasion of these alien plants in the ecosystem threatens the habitat of endemic species, reducing biodiversity, and causing a disturbance in the ecological system. In urbanized areas of campus universities, a diverse range of organisms were found and a comparison between the sites, near roads or housing sites were made because the campuses provided a large biotope. Although the campus had been exposed to interferences like gardening, it was also a place for most organisms to live in an active floating population due to free access. This research investigated the flora of alien plants that appeared in Beakseok University, Sangmyung University, Hoseo University and Dankook University, and relationship between the distribution of alien plants and the campus and green areas and distance from the highway. The total number of plant species and naturalized species found in the four universities was 189 and 43 species. Those of Dankook University were 136 and 35 species, Hoseo 108 and 25 species, Sangmyung 103 and 31 species, and Baekseok 97 and 26 species, respectively. The abundance of natural plants for each respective university tended to be higher as it became closer to the highway. Also, the closer the walking distance to the university, the greater the tendency for the degree of similarity to be higher. As a result, we may conclude that the distribution of alien plants and anthropogenic activities may be closely related.

• Journal of the korean academy of Pediatric Dentistry
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• v.45 no.1
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• pp.41-56
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• 2018

The purpose of this study was to provide clinical recommendations for restoration with selection of the most similar zirconia crown by 3-dimensional analysis of the shape of the maxillary primary central and lateral incisors in Korean individuals and prefabricated zirconia crowns. The average shape of the sound maxillary primary central and lateral incisors in 300 children was reproduced by 3-dimensional scanning. Zirconia crowns of 4 manufacturers (NuSmile $ZR^{(R)}$ Crown, Cheng $Crowns^{(R)}$, Kinder $Krowns^{(R)}$, and EZ $Pedo^{(R)}$ Crown) were scanned 3-dimensionally, and coordinates for comparison of the shape were measured to evaluate the similarity between the teeth and crowns. The most similar crowns were selected by comparing the mesiodistal length, crown height, crown shape ratio, distance between the same coordinates of a tooth and crown, the radius of curvature of the labial surface, and the volume. As a result of analysis, Cheng $Crowns^{(R)}$ size 3 and NuSmile $ZR^{(R)}$ Crown size 2 were the most similar crowns in the maxillary primary central and lateral incisors, respectively. Scanning the inner surface of the crowns and evaluating the amount of tooth reduction required suggested that an overall lesser amount of tooth reduction compared to that presented by the manufacturer's guidelines should be performed.

• Journal of the Korean Geophysical Society
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• v.10 no.1
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• pp.1-12
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• 2007

A method to quickly estimate broadband moment magnitudes (Mwp) to warn regional and teleseismic tsunamigenic earthquakes is tested for application of the method to the different seismic observation environment. In this study, the Mwp is calculated by integrating far-field P-wave or pP-wave of vertical component of displacement seismograms in time domain from earthquakes, having magnitude greater than 5.0 and occurred in and around the Korean peninsula from 2000 to 2006. We carefully set up the size of the time window for the computations to exclude S wave phases and other phases following after the P wave phase. The P wave velocities and the densities from the averaged Korean crustal model are used in the computations. Instrumental correction was performed to remove dependency on the seismograph. The Mwp after the instrumental correction is about 0.1 greater than the Mwp before the correction. The comparison of our results to the those of foreign agencies such as JMA and Havard CMT catalogues shows a higher degree of similarity. Thus our results provide an effective tool to estimate the earthquake size, as well as to issue the necessary information to a tsunami warning system when the effective earthquake occurs around the peninsula.

• Geophysics and Geophysical Exploration
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• v.12 no.1
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• pp.154-162
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• 2009

Sonic wave dispersion characteristics are one of the most important targets of study, particularly in estimating shear wave velocity from borehole sonic logging. We have tested dispersion characteristics using monopole and dipole sources. Theoretical dispersion curves were computed for tool-absent and tool-included models having the same physical properties but different diameters (including ${\Phi}520mm$, ${\Phi}150mm$, and ${\Phi}76mm$). Comparisons were made between boreholes of different sizes and between tool-absent and tool-included models. Between the tool-included and the tool-absent boreholes, a close similarity in dispersion curve shape was revealed for the monopole source, and a significant difference was shown for the dipole source. However, for the cut-off frequency, particularly in the engineering boreholes (${\Phi}76mm$ and ${\Phi}50mm$), a significant difference was observed for signals from the monopole source, but approximately the same cut-off frequencies were found with the dipole source. This indicates the need of careful choice of source frequency in monopole-source sonic logging, particularly in an engineering borehole. The results of numerical experiments show that cut-off frequency is exponentially proportional to the inverse of borehole radius, irrespective of the mode type and the presence of a tool, and that the cut-off frequencies for each borehole environment could be expressed as an exponential function, rather than the inversely proportional relationship between the cut-off frequency and the borehole radius that was previously generally recognised. From the direct comparison of dispersion curves, the effects on the dispersion characteristics of borehole size and the presence of the tool can be revealed more clearly than in previous studies, which presented the dispersion curve and/or characteristics for each borehole environment separately.