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The Experimental Study on the Anti-Allergic Rhinitis Effects of the Sinichengpae-um (신이청매음(辛夷淸肺飮)이 알레르기 비염(鼻炎)에 미처는 효과(效果)에 대한 실험적(實驗的) 연구(硏究))

  • Kang, Sang-Hun;Sim, Sung-Yong;Byun, Hak-Sung;Kim, Kyung-Jun
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.18 no.3
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    • pp.18-25
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    • 2005
  • Objectives: It has a growing interest in the prevention and medical treatment of allergic rhinitis. According to many studies, it's known that Sinicheongpye-um has the inhibitory effect on the allergic rhinitis. We have studied effect of the mice on OVA-induced Production of IL-4, IL-5, $Interferone-{\gamma}$ by Murine Splenocytes and effect of OVA-induced Total IgE. Methods: Mixing Ovalbumin(OVA) $10{\mu}g$ into PBS(phoshate buffered saline) and $Al(OH)_3$, gel solution and changing into $1\;m{\ell}$, we made it into OVA solution. That was administered to normal group. After the last administration into abdominal cavity, we caused allergic rhinitis in nasal cavity of mouse of control group and sample group administering 0.1% solution dropwise 3 times a day for 7 days. Keeping separated serum at -20 degree and after refloating spleen cells, cultivating the cells and centrifuging the upper liquid and keeping it at -20 degree, we measured the amount of IL-4. IL-5, $Interferone-{\gamma}$ and OVA-induced Total IgE by ELISA. Results: 1. In Total IgE, Sinichengpae-um treated group was proved significant inhibitory effect.(p<0.05) 2. In IL-4 study, Sinichengpae-um treated group was proved significant inhibitory effect.(p<0.005) 3. In IL-4 study, Sinichengpae-um treated group was proved significant inhibitory effect.(p<0.001) 4. In $Interferone-{\gamma}$ study, Sinichengpae-um treated group showed a increasing tendency. Conclusion: Based on the above result, it is considered that Sinichengpae-um has the inhibitory effect on the allergic rhinitis of mice and suggested thai it could be used in relieving patients of the symptoms which are caused by allergic rhinitis.

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LAMP-3 (Lysosome-Associated Membrane Protein 3) Promotes the Intracellular Proliferation of Salmonella typhimurium

  • Lee, Eun-Ju;Park, Kwan-Sik;Jeon, In-Sook;Cho, Jae-Woon;Lee, Sang-Jeon;Choy, Hyun E.;Song, Ki-Duk;Lee, Hak-Kyo;Choi, Joong-Kook
    • Molecules and Cells
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    • v.39 no.7
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    • pp.566-572
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    • 2016
  • Lysosomes are cellular organelles containing diverse classes of catabolic enzymes that are implicated in diverse cellular processes including phagocytosis, autophagy, lipid transport, and aging. Lysosome-associated membrane proteins (LAMP-1 and LAMP-2) are major glycoproteins important for maintaining lysosomal integrity, pH, and catabolism. LAMP-1 and LAMP-2 are constitutively expressed in Salmonella-infected cells and are recruited to Salmonella-containing vacuoles (SCVs) as well as Salmonella- induced filaments (Sifs) that promote the survival and proliferation of the Salmonella. LAMP-3, also known as DC-LAMP/CD208, is a member of the LAMP family of proteins, but its role during Salmonella infection remains unclear. DNA microarray analysis identified LAMP-3 as one of the genes responding to LPS stimulation in THP-1 macrophage cells. Subsequent analyses reveal that LPS and Salmonella induced the expression of LAMP-3 at both the transcriptional and translational levels. Confocal Super resolution N-SIM imaging revealed that LAMP-3, like LAMP-2, shifts its localization from the cell surface to alongside Salmonella. Knockdown of LAMP-3 by specific siRNAs decreased the number of Salmonella recovered from the infected cells. Therefore, we conclude that LAMP-3 is induced by Salmonella infection and recruited to the Salmonella pathogen for intracellular proliferation.

Rapid Micropropagation by Axillary Buds Cultures of Smilax china

  • Song, Hyun-Jin;Sim, Seon-Jeong;Jeong, Mi-Jin;Heo, Chang-Mi;Kim, Hak-Gon;Jeong, Gwon-Yong;Heo, Su-Yeoung;Choi, Yong-Weon;Park, Geun-Hye;Yang, Jae-Kyung;Moon, Hyun-Shik;Choi, Myung-Suk
    • Journal of agriculture & life science
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    • v.44 no.6
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    • pp.39-44
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    • 2010
  • An efficient method for the rapid propagation of Smilax china from axillary buds was established. Plants with thick leafage were selected from Korea native S. china population. Axillary buds of S. china collected from selected plant and were cultured in various culture media (2MS, MS, 1/2MS, WPM, B5 and SH medium). Shoot was induced from axillary bud on MS basal medium after 4 weeks of culture. 1/2MS medium showed a higher growth rate than those of the others, while the lowest shoot growth was obtained in 2MS medium. Among the sucrose concentrations, 5% sucrose was the optimum level for shoots growth from axillay buds. Among cytokinins, $0.5mgL^{-1}$ 6-benzylaminopurine (BAP) treatment showed the best performance on shoot multiplication, yielding average shoot multiplication forming about 2.4. Rooting was induced directly near the base of the shoot on 1/2MS medium containing with three-auxins ${\alpha}-napthalene$ acetic acid (NAA), indole acetic acid (IAA) and ${\beta}-indolebutyric$ acid (IBA) (0.5 and $1.0mgL^{-1}$). The $1.0mgL^{-1}$ IBA treatments induced earliest rooting with maximum of root number and root growth. These rooted plantlets were successfully transferred to pots for 4 weeks hardening process, and were transferred to soil with above 90% survival rate.

Enzymatic Saccharification of Salix viminalis cv. Q683 Biomass for Bioethanol Production

  • Kim, Hak-Gon;Song, Hyun-Jin;Jeong, Mi-Jin;Sim, Seon-Jeong;Park, Dong-Jin;Yang, Jae-Kyung;Yoo, Seok-Bong;Yeo, Jin-Ki;Karigar, Chandrakant S.;Choi, Myung-Suk
    • Journal of Forest and Environmental Science
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    • v.27 no.3
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    • pp.143-149
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    • 2011
  • The possibility of employing biomass of Salix viminalis cv. Q683 as a resource of bio-energy was evaluated. The chemical analysis of S. viminalis cv. Q683 leaf biomass showed components such as, extractives (2.57%), lignin (39.06%), hemicellulose (21.61%), and cellulose (37.83%), whereas, its stem was composed of extractives (1.67%), lignin (23.54%), hemicellulose (33.64%), and cellulose (42.03%). The biomass of S. viminalis cv. Q683 was saccharified using two enzymes celluclast and viscozyme. The saccharification of S. viminalis cv. Q683 biomass was influenced by enzymes and their strengths. The optimal enzyme combination was found to be celluclast (59 FPU/g substrate) and viscozyme (24 FBG/g substrate). On saccharification the glucose from leaf and stem biomass was 7.5g/L and 11.7g/L, respectively after 72 hr of enzyme treatment. The biomass and enzyme-treated biomass served as the feedstock for ethanol production by fermentation. The ethanol production from stem and leaf biomass was 5.8 g/L and 2.2 g/L respectively, while the fermentation of the enzymatic hydrolysates yielded 5 g/L to 8 g/L bioethanol in 72 hours.

Effects of inorganic salts on biomass production, cell wall components, and bioethanol production in Nicotiana tabacum

  • Sim, Seon Jeong;Yong, Seong Hyeon;Kim, Hak Gon;Choi, Myung Suk;Choi, Pil Son
    • Journal of Plant Biotechnology
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    • v.48 no.4
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    • pp.278-288
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    • 2021
  • The development of bioenergy through biomass has gained importance due to the increasing rates of fossil fuel depletion. Biomass is important to increase the productivity of bioethanol, and production of biomass with high biomass productivity, low lignin content, and high cellulose content is also important in this regard. Inorganic salts are important in the cultivation of biomass crops for the production of biomass with desirable characteristics. In this study, the roles of various inorganic salts in biomass and bioethanol production were investigated using an in vitro tobacco culture system. The inorganic salts evaluated in this study showed dramatic effects on tobacco plant growth. For example, H2PO4 substantially improved plant growth and the root/shoot (R/S) ratio. The chemical compositions of tobacco plants grown in media after removal of various inorganic salts also showed significant differences; for example, lignin content was high after Mg2+ removal treatment and low after K+ treatment and H2PO4 removal treatment. On the other hand, NO3- and H2PO4 treatments yielded the highest cellulose content, while enzymatic hydrolysis yielded the highest glucose concentration ratio 24 h after NH4+ removal treatment. The ethanol productivity after H2PO4 removal treatment was 3.95% (w/v) 24 h after fermentation and 3.75% (w/v) after 36 h. These results can be used as the basis for producing high-quality biomass for future bioethanol production.

A Study on PCS for ML-Based Electrical Propulsion System (ML 기반의 전기추진시스템을 위한 PCS에 관한 연구)

  • Lee, Jong-Hak;Lee, Hun-Seok;Oh, Jin-Seok
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.23 no.9
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    • pp.1025-1031
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    • 2019
  • This study proposes a PCS that enables efficient operation of seawater pumps for ships by implementing ML-based algorithms. Seawater temperature, RPM and power consumption data are acquired from two ships with PCS, analyzed with regression analysis method, and new algorithms are presented. Using the algorithms presented, Ship A saved about 36% compared to the PCS application, and ML-based algorithms in certain sea temperatures of 19 to 27 degrees Celsius and above 32 degrees Celsius were about 1% lower than Ship A's PCS. Ship B saved about 50% compared to PCS not applied, and about 2% more than Ship B's PCS in waters above $19^{\circ}C$, a specified sea temperature. The derived data can be used to suggest the optimum pump speed and sea route. In addition, the trend of acquired data can be used to infer the performance of the pump or the timing of elimination of the MGPS when efficiency becomes poor.

Adsorption and Desorption Characteristics of Sr, Cs, and Na Ions with Na-A Zeolite Synthesized from Coal Fly Ash in Low-Alkali Condition (석탄 비산재로부터 저알칼리 조건에서 합성된 Na-A 제올라이트의 Sr, Cs 및 Na 이온의 흡탈착 특성)

  • Choi, Jeong-Hak;Lee, Chang-Han
    • Journal of Environmental Science International
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    • v.28 no.6
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    • pp.561-570
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    • 2019
  • A zeolitic material (Z-Y2) was synthesized from Coal Fly Ash (CFA) using a fusion/hydrothermal method under low-alkali condition (NaOH/CFA = 0.6). The adsorption performance of the prepared zeolite was evaluated by monitoring its removal efficiencies for Sr and Cs ions, which are well-known as significant radionuclides in liquid radioactive waste. The XRD (X-ray diffraction) patterns of the synthesized Z-Y2 indicated that a Na-A type zeolite was formed from raw coal fly ash. The SEM (scanning electron microscope) images also showed that a cubic crystal structure of size $1{\sim}3{\mu}m$ was formed on its surface. In the adsorption kinetic analysis, the adsorption of Sr and Cs ions on Z-Y2 fitted the pseudo-second-order kinetic model well, instead of the pseudo-first-order kinetic model. The second-order kinetic rate constant ($k_2$) was determined to be $0.0614g/mmol{\cdot}min$ for Sr and $1.8172g/mmol{\cdot}min$ for Cs. The adsorption equilibria of Sr and Cs ions on Z-Y2 were fitted successfully by Langmuir model. The maximum adsorption capacity ($q_m$) of Sr and Cs was calculated as 1.6846 mmol/g and 1.2055 mmol/g, respectively. The maximum desorption capacity ($q_{dm}$) of the Na ions estimated via the Langmuir desorption model was 2.4196 mmol/g for Sr and 2.1870 mmol/g for Cs. The molar ratio of the desorption/adsorption capacity ($q_{dm}/q_m$) was determined to be 1.44 for Na/Sr and 1.81 for Na/Cs, indicating that the amounts of desorbed Na ions and adsorbed Sr and Cs ions did not yield an equimolar ratio when using Z-Y2.

Measurement of Fingerprint Image Quality using Hybrid Segmentation method (Hybrid Segmentation을 이용한 Fingerprint Image Quality 측정 방법)

  • Park, Noh-Jun;Jang, Ji-Hyeon;Kim, Hak-Il
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.17 no.6
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    • pp.19-28
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    • 2007
  • The purpose of this paper is to present a new measure for fingerprint image quality assessment that has a considerable effect on evaluation of fingerprint databases. This paper introduces a hybrid segmentation method for measuring an image quality and evaluates the experimental results using various fingerprint databases. This study compares the performance of the proposed hybrid segmentation using variance and coherence of fingerprints against the NIST's NFIQ program. Although NFIQ is a most widely used tool, it classifies the image quality into 5 levels. However, the proposed hybrid method is developed to be conformant to the ISO standards and accordant to human visual perception. The experimental results demonstrate that the hybrid method is able to produce finer quality measures.

Development of signal linkage simulator for verification of Ships energy management system algorithm (선박용 에너지 관리 시스템 알고리즘 검증을 위한 신호 연동 시뮬레이터 개발)

  • Lee, Jong-Hak;Oh, Ji-Hyun;Sim, Jae-Soon;Oh, Jin-Seok
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.26 no.6
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    • pp.881-889
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    • 2022
  • As interest in environmental pollution caused by ship emissions is increasing worldwide, many studies are being conducted on the development of systems that increase energy efficiency to reduce ship emissions. In order to test the energy management system controller driven in real time on a large ship, it is necessary to perform the test through the simulator. In this study, addresses were set for each signal according to Modbus TCP/IP so that each control system and energy management system could be linked, and the algorithm was configured according to the signal flow. In addition, the signal generator was designed and manufactured so that each controller could artificially generate the signal collected from the vessel. As a result of the simulator production and interlocking, it was confirmed that each controller operated in real time performed its role appropriately, and that the algorithm of the ship energy management system was properly applied.

How to Protect Critical Nodes of Megacities in Preparation for the Digitization of Spatial Information (공간정보 디지털화에 대비한 메가시티 핵심노드 방호 대책)

  • Sim, Jun Hak;Cho, Sang Keun;Park, Sung Jun;Park, Sang-Hyuk
    • The Journal of the Convergence on Culture Technology
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    • v.8 no.4
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    • pp.121-125
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    • 2022
  • As the technologies of the 4th industrial revolution develops, spatial information is becoming digitized. Now, even with a smartphone, we can easily identify the location of national & military critical facilities located in the mega cities. As a result, mega cities' national & military critical facilities were exposed to not only traditional threats, but also non-traditional threats such as terrorism, cyber hacking, and criminal activities. This study suggests a way to protect national & military critical facilities of mega cities from such threats. Considering limitation of time & resources, protecting perfectly all national & military critical facilities is impossible, so we should focus on their critical nodes. Specifically, We suggest ways to protect the critical nodes by converging some measures such as design & arrangement in harmony with the surrounding environment, underground construction & covering, and visual deception. Transparency of digital spatial information will further increase with the advent of urban air mobility and autonomous vehicles in the future. Therefore, in order to prepare for future threats, we should take measures to minimize the exposure of critical nodes.