Journal of Forest and Environmental Science

Institute of Forest Science, kangwon National University (강원대학교 산림과학연구소)
권호 표지
DOI QR코드

DOI QR Code

Enzymatic Saccharification of Salix viminalis cv. Q683 Biomass for Bioethanol Production

  • Kim, Hak-Gon (Division of Environmental Forest Science, Gyeongsang National University & Institute of Agriculture of Life Science) ;
  • Song, Hyun-Jin (Division of Environmental Forest Science, Gyeongsang National University & Institute of Agriculture of Life Science) ;
  • Jeong, Mi-Jin (Division of Environmental Forest Science, Gyeongsang National University & Institute of Agriculture of Life Science) ;
  • Sim, Seon-Jeong (Division of Environmental Forest Science, Gyeongsang National University & Institute of Agriculture of Life Science) ;
  • Park, Dong-Jin (Division of Environmental Forest Science, Gyeongsang National University & Institute of Agriculture of Life Science) ;
  • Yang, Jae-Kyung (Division of Environmental Forest Science, Gyeongsang National University & Institute of Agriculture of Life Science) ;
  • Yoo, Seok-Bong (Korea Forest Research Institute) ;
  • Yeo, Jin-Ki (Departments of Forest Resources Development, Korea Forest Research Institute) ;
  • Karigar, Chandrakant S. (Department of Biochemistry, Bangalore University) ;
  • Choi, Myung-Suk (Division of Environmental Forest Science, Gyeongsang National University & Institute of Agriculture of Life Science)
  • Received : 2011.11.09
  • Accepted : 2011.12.22
  • Published : 2011.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The possibility of employing biomass of Salix viminalis cv. Q683 as a resource of bio-energy was evaluated. The chemical analysis of S. viminalis cv. Q683 leaf biomass showed components such as, extractives (2.57%), lignin (39.06%), hemicellulose (21.61%), and cellulose (37.83%), whereas, its stem was composed of extractives (1.67%), lignin (23.54%), hemicellulose (33.64%), and cellulose (42.03%). The biomass of S. viminalis cv. Q683 was saccharified using two enzymes celluclast and viscozyme. The saccharification of S. viminalis cv. Q683 biomass was influenced by enzymes and their strengths. The optimal enzyme combination was found to be celluclast (59 FPU/g substrate) and viscozyme (24 FBG/g substrate). On saccharification the glucose from leaf and stem biomass was 7.5g/L and 11.7g/L, respectively after 72 hr of enzyme treatment. The biomass and enzyme-treated biomass served as the feedstock for ethanol production by fermentation. The ethanol production from stem and leaf biomass was 5.8 g/L and 2.2 g/L respectively, while the fermentation of the enzymatic hydrolysates yielded 5 g/L to 8 g/L bioethanol in 72 hours.

Keywords

References

  1. Belkacemi, K., Turcotte, G., de Halleux, D. and P. Savoie. 1998. Ethanol production from AFEX-treated forages and agricultural residues. Appl. Biochem. Biotechnol. 70-72: 441-462. https://doi.org/10.1007/BF02920159
  2. Bjerre, A.B., Olesen, A.B. and T. Fernqvist. 1996. Pretreatment of wheat straw using combined wet oxidation and alkaline hydrolysis resulting in convertible cellulose and hemicellulose. Biotechnol and Bioeng. 49: 568-577. https://doi.org/10.1002/(SICI)1097-0290(19960305)49:5<568::AID-BIT10>3.3.CO;2-4
  3. Bothast, R.J. and M.A. Schlicher. 2005. Biotechnological processes for conversion of corn into ethanol. Appl. Microbiol. and Biotech. 67: 19-25. https://doi.org/10.1007/s00253-004-1819-8
  4. Dale, B.E., Henk, L.L. and M. Shiang. 1984. Fermentation of lignocellulosic materials treated by ammonia freeze-explosion. Developments in Industrial Microbiology. 26: 223-233.
  5. Ding, S.Y. and M.E. Himmel 2006. The maize primary cell wall microfibril: a new model derived from direct visualization. J. Agri. and Food Chem. 54: 597-606. https://doi.org/10.1021/jf051851z
  6. Duff, S.J.B. and W.D. Murray. 1996. Bioconversion of forest products industry waste cellulosics to fuel ethanol: a review. Bioresource Technology. 55: 1-33. https://doi.org/10.1016/0960-8524(95)00122-0
  7. Fox, D.J., Gray, P.P., Dunn, N.W. and L.M. Warwick. 1989. Comparison of alkali and steam (acid) pretreatments of lignocellulosic materials to increase enzymatic susceptibility: evaluation under optimized pretreatment conditions. J. of Chemical Tech. & Biotech. 44: 135-146.
  8. Hayn, M., Steiner, W., Klinger, R., Steinmüller, H., Sinner, M. and H. Esterbauer. 1993. Basic Research and Pilot Studies on the Enzyzmatic Conversion of Lignocellulosics. ed. J.N. Saddler, Bioconversion of Forest and Agricultural Plant Residues. CAB International, Wallingford, UK. pp. 33-72.
  9. Helby, P.R. and H.A. Roos. 2006. Retreat from Salix - Swedish experiences with energy crops in the 1990s. Biomass & Bioenergy. 30: 422-427. https://doi.org/10.1016/j.biombioe.2005.12.002
  10. Heinsoo, K.M., Petrovits, E.M. and A. Koppel. 2009. Fine root biomass and production in a Salix viminalis and Salix dasyclados plantation. Estonian J. of Ecology. 59: 27-37.
  11. Hu Z., Foston, M. and A.J. Ragauskas. 2011. Comparative studies on hydrothermal pretreatment and enzymatic saccharification of leaves and internodes of alamo switchgrass. Bioresource Technology. 102(14): 7224-7228 https://doi.org/10.1016/j.biortech.2011.04.029
  12. Koo, B.W., Park, N.H., Yeo, H.M., Kim, H. and I.G. Choi. 2009. Study on affecting variables appearing through chemical pretreatments of poplar wood to enzymatic hydrolysis. Mokchae Konhak. 37(3): 255-264.
  13. Koo, W.W. and R.D. Taylor. 2009. 2009 Outlook of the U.S. and World Sugar Markets 2008-2018. Agribusiness & Applied Economic Report, No. 646.
  14. MacDonald, D.G., Bakhshi, N.N., Mathews, J.F. and A. Roychowdhury. 1983. Alkali treatment of corn stover to improve sugar production by enzymatic hydrolysis. Biotechnol. and Bioeng. 25: 2067-2076. https://doi.org/10.1002/bit.260250815
  15. Merino, S.T. and J. Cherry. 2007. Progress and challenges in enzyme development for biomass utilization. Adv. Biochem. Eng. Biotechnol. 108: 95-120.
  16. Ramos, L.P., Breuil, C. and J.N. Saddler. 1992. Comparison of steam pretreatment of eucalyptus, aspen, and spruce wood chips and their enzymatic hydrolysis. Appl. Microbiol. and Biotech. 34/35: 37-48.
  17. Silverstein, R.A., Chen, Y., Sharma-Shivappa, R.R., Boyette, M.D., and J. Osborne. 2007. A comparison of chemical pretreatment methods for improving saccharification of cotton stalks. Bioresource Technology. 98: 3000-3011. https://doi.org/10.1016/j.biortech.2006.10.022
  18. Sørensen, A., Teller, P.J., Hilstrom, T. and B.K. Ahring. 2008. Hydrolysis of Miscanthus for bioethanol production using dilute acid presoaking combined with wet explosion pretreatment and enzymatic treatment. Bioresource Technology. 99: 6602-607. https://doi.org/10.1016/j.biortech.2007.09.091
  19. Sun, F.B. and H.Z. Chen. 2008b. Organosolv pretreatment by crude glycerol from oleochemicals industry for enzymatic hydrolysis of wheat straw. Bioresource Technology. 99: 5474-5479. https://doi.org/10.1016/j.biortech.2007.11.001
  20. Wiselogel, A., Tyson, S. and D. Johnson. 1996. Biomass Feedstock Resources and Composition, ed. C.E. Wyman, Handbook on Bioethanol: Production and Utilization (Applied Energy Technology Series), CRC press, Washington DC. pp105-118.
  21. Wright, J.D. 1998. Ethanol from biomass by enzymatic hydrolysis. Chemical Engineering Progress. 84: 62-74.
  22. Xu, J., Cheng, J.J., Sharma-Shivappa, R.R., and J.C. Burns. 2010. Lime pretreatment of switchgrass at mild temperatures for ethanol production. Bioresource Technology. 101: 2900-2903. https://doi.org/10.1016/j.biortech.2009.12.015