• Journal of Sericultural and Entomological Science
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• v.30 no.2
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• pp.105-111
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• 1988

견 피브로인과 그 응용에 관한 기초적인 자료를 얻기 위하여, 본 연구에서는, 견섬유와 피브로인막의 구조에 대해서 IR, DSC, X-ray diffraction, SEM, 아미노산분석 등의 조사를 통하여 구조변화를 비교하였다. IR과 DSC를 통하여 얻은 결과, 피브로인 막의 구조는 대부분 random coil과 Silk I type으로 구성되어 있지만, Silk II type의 존재도 인정되었다. 피브로인 막을 전자현미경으로 관찰한 결과, 표면은 구상체로 치밀하게 형성되어 있었고, 단면구조는 거의 대칭적인 것임을 알았다. 견섬유와 피브로인 막의 아미노산 분석결과, 전체에 대한 염기성 아미노산의 함량은 피브로인 막보다 견섬유가 더 많은 것을 알 수 있다.

• Journal of Life Science
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• v.10 no.5
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• pp.511-518
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• 2000

This study was designed to investigate the effects of silk fibroin powder (SFP : Mw 500) on oxidative stress and membrane fluidity in brain membranes of rats. Sprague-Dawley (SD) male rats (160$\pm$10 g) were fed basic diet (control group), and experimental diets (SFP-2.5 and SFP-5.0 groups) added 2.5 and 5.0 g/kg BW/day for 6 weeks. Cholesterol level was significantly decreased about 8.0% in brain microsomes of SFP-5.0 group only compared with control group. Membrane fluidities were significantly increased (12.9% and 15.2%, respectively) in brain microsomes of SFP-2.5 and SFP-5.0 groups, but significant difference between in brain mitochondria of these two groups could be not obtained. Basal oxygen radicals (BOR) in brain mitochondria and microsomes were significantly ingibited (10.4%, and 24.0%, 7.9% and 14.9%, respectively) by SFP-2.5 and SFP-5.0 groups compared with control group. Induced oxygen radicals (IOR) in brain mitochondria and microsomes were significantly inhibited (11.8% and 14.1%, respectively) by SFP-5.0 groups compared with control group compared with control group. Lipid peroxide (LPO) levels were dose-dependently decreased (12.9% and 21.9%, 13.2% and 22.5%, respectively) in brain mitochondria and microsomes of SFP-2.5 and SFP-5.0 groups compared with control group. Oxidized protein (OP) levels were significantly decreased (15.7% and 17.1%, 16.7% and 15.7%, respectively) in brain mitochondria and microsomes of SFP-2.5 and SFP-2.5 and SFP-5.0 groups compared with control group. These results suggest that administration of SFP may play an effective role in a attenuating a oxidative stress and increasing a membrane fluidity in brain membranes.

• Journal of Sericultural and Entomological Science
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• v.37 no.2
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• pp.142-153
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• 1995

This study is undertaken to investigate proper condition and dissolution method of silk fibroin to use it functional material as powder or membrane. Silk fibroin was dissolved with calcium chloride ethanol aqueous solution and hydrochloric acid. When silk fibron was dissolved with calcium chloride ehanol aqueous solution, main chain of silk fibroin was degradaded and molecular conformation was changed. Silk fibroin powder was made from silk fibroin solution. It showed lower thermal decomposition temperature and crystallinity than those of native silk fibroin. And Its molecular conformation was random coil structure. By acid gydrolysis, main chain of silk fibroin was attacked randomly. Silk fibroin powder from hydrolysate showed high crystallinity and thermal decomposition temprature. $\beta$-form molecular conformation was found by IR and X-ray diffraction. Silk fibroin powder form dissolved part with hydrochloric acid showed low thormal decomposition temperature but high crystallinity. During acid hydrolysis, transition of molecular structure of silk fibroin occurred, and it changed to $\alpha$-helix. Silk fibroin film was achieved by casting silk fibroin solution by ehanol solution or saturated vapor treatment, and its molecular conformation changed to $\beta$structure.

• Membrane Journal
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• v.17 no.4
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• pp.375-380
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• 2007

To recover sericin protein from by-product in silk production process, a polyethersulfone hollow fiber ultra-filtration membrane module was used. The soap in the degummed solution was precipitated by calcium chloride. The influence of membrane module of submerged and external type on membrane fouling was investigated. The effect of soap and protein on the membrane fouling in the external type membrane module was also studied. The removal of soap resulted in decreasing the membrane fouling. It was shown that the protein and the membrane were affected by the soap.

• International Journal of Industrial Entomology and Biomaterials
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• v.27 no.1
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• pp.209-217
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• 2013

The objective of this study was to evaluate the bone regeneration capability of silk membrane plus 3% 4-hexylresorcinol (3% 4-HR plus SM) in a rabbit calvarial defect model. Twenty New Zealand white rabbits were used in this study. Bilateral round shaped defects were created in the parietal bone (diameter: 8.0 mm). And the defects were covered with (1) 3% 4-HR plus SM, (2) collagen membrane (CM), (3) no graft material. After surgery, the animals were sacrificed at 4 weeks and 8 weeks. Bone regeneration was analyzed in each section by micro-computerized tomography (${\mu}$-CT). And Hematoxylin and eosin stains were used for histological analysis. As measured by ${\mu}$-CT analysis 4 weeks after surgery, the average of new bone formation in animals treated with 3% 4-HR plus SM was greater than that of animals treated with CM. and the difference was statistically significant. And well organized lamella bones were observed in the histological view of the 3% 4-HR plus SM group. Therefore, more bone regeneration was seen in animals treated with 3% 4-HR plus SM than in those treated with CM or uncovered control.

• Journal of Sericultural and Entomological Science
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• v.52 no.2
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• pp.89-95
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• 2014

Silk polymer has been focused recently on medical applications as a novel biomaterials. The factors for biomaterials were considered and reviewed recent research on surgical suture, wound dressing, tympanic regeneration patch, guided bone regeneration membrane, bone implant and ligament. Silk polymer is good mechanical properties and biocompatibility. Therefore it might be used as one of promising materials for medical application.

• International Journal of Industrial Entomology and Biomaterials
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• v.22 no.2
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• pp.101-105
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• 2011

Transparency and insolubility of eardrum patch against exudates are important for otolaryngological surgery. The author prepared silk fibroin (SF) films with various concentrations and temperature and then examined solubility and conformation of SF films. SF films were transparent regardless of the various preparation conditions. Although most SF films are soluble in 1X PBS solution at $37^{\circ}C$ for 1 h, the SF film with 3.4% with $60^{\circ}C$ was insoluble. Scanning electron microscopy (SEM) showed that the SF films have solid and smooth surface. Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC) results showed that the conformation of SF films was influenced by the preparation conditions including SF concentration and casting temperature. In conclusion, SF membrane with transparence and insolubility against exudates could be considered as eardrum patch resources.

• Membrane Journal
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• v.31 no.2
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• pp.153-159
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• 2021

In recent years, polymer membranes, which are actively used in various industrial fields, have the advantage of being able to impart unique properties through the control of chemical structures and physical properties in the film-fabrication process, as well as through fabricating blend membranes mixed with various materials. In this study, the solubility parameter, which can be used as an index of miscibility with other materials, was calculated using molecular dynamics using a silkworm (Bombyx mori) silk polymer which has a wide potential to be used as an eco-friendly natural material. When the solubility parameter of polyvinylalcohol (PVA), which is also environmentally friendly and biocompatible, was calculated by molecular dynamics and compared with each other, it was confirmed that the two polymer materials had similar solubility parameter values. In conclusion, it was theoretically proved that the two polymers could blend well with each other, which was confirmed through experiments.

• Textile Coloration and Finishing
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• v.10 no.5
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• pp.56-62
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• 1998

In the prior study, the dyeing behaviors of Milling Cyanine 5R on silk fiber in aqueous dyeing liquor including small amount of organic compounds were investigated. The most effective compound was acetophenone which increased dye uptake as well as dyeing rate. In this study, the role of the acetophenone in dyeing of silk with Milling Cyanine 5R was studied. By addition of acetophenone into the dye solution, the molar absorptivity of Milling Cyanine 5R increased, and the wavelength of maximum absorption was shifted to longer wavelength, namely bathochromic shifted. This shows that the acetophenone prevents the dye to aggregate, and increases the number of monomeric dye molecule relative to the dye solution without acetophenone. This fact was also confirmed by the increase of the permeation rate of the dye through cellulose semipermeable membrane from the dye solution including the acetophenone. From these results, the acetophenone acts as a prohibitor of dye aggregation, increases the number of monomeric dye molecules even at relatively low temperature, and makes the dyes penetrate into the fiber easier.

• Journal of Sericultural and Entomological Science
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• v.42 no.1
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• pp.58-64
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• 2000

This study was designed to investigate the effects of silk fibroin powder (Mw 500) on oxidative stress and membrane fluidity in liver membranes of rats. Sprague-Dawley (SD) male rats (160$\pm$10g) were fed basic diet (control group), and experimental diets (SEP-2.5 and SFP-5.0 groups) added 2.5 and 5.0 g/kg BW/day for 6 weeks. Cholesterol levels resulted in a significant decrease (12.1% and 9.0%, respectively) in the liver mitochondria and microsomes of SEP-5.0 group compared with control group. Membrane fluidity as significantly increased (16.1% and 16.5%, 5.8% and 17.4%) in the liver mitochondria and microsomes were significantly inhibited (16.1% and 18.3%, 8.1% and 15.1%, respectively) at the SFP-2.5 and SEP-5.0 groups compared with control group. Induced oxygen radicals (BOR) in liver mitochondria and microsomes were significantly inhibited (16.1% and 18.3%, 8.1% and 15.1%, respectively) at the SFP-2.5 and SEP-5.0 groups compared with control group. Induced oxygen radicals (IOR) in liver microsomes were significantly inhibited (17.0% and 26.6%, respectively) at the SFP-2.5 and SFP-5.0 groups compared with control group, but IOR in liver mitochondria was significantly inhibited about 12.3% at the SWP-400 group only compared with control group. Lipid peroxide (LPO) levels were significantly decreased (8.3% and 18.0%, 13.4% and 18.4%, respectively) in the liver mitochondria and microsomes of SFP-2.5 and SFP-5.0 groups compared with control group. Oxidized protein (OP) levels were dose-dependently decreased (5.4% and 11.6%, 19.0% and 24.4%, respaectively) in the iver mitochondria and microsomes of SFP-2.5 and SFP-5.0 groups compared with control group. These results suggest that administration of SFP may play an effective role in attenuating an oxidative stress and increasing a membrane fluidity in liver membranes.