International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Solubility and Conformation of Silk Fibroin Membrane

  • Kweon, Hae-Yong (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Kang, Seok-Woo (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Lee, Kwang-Gill (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Park, Kwang-Young (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Jo, You-Young (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Kang, Pil-Don (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Sung, Gyoo-Byung (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Lee, Heui-Sam (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Yeo, Joo-Hong (Rural Development Administration) ;
  • Shin, Kyu-Hwan (Uljin Agricultural Technology Center) ;
  • Park, Chan-Hum (Department of Otorhinolaryngology, Hallym University)
  • Received : 2011.04.12
  • Accepted : 2011.05.06
  • Published : 2011.06.30
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Abstract

Transparency and insolubility of eardrum patch against exudates are important for otolaryngological surgery. The author prepared silk fibroin (SF) films with various concentrations and temperature and then examined solubility and conformation of SF films. SF films were transparent regardless of the various preparation conditions. Although most SF films are soluble in 1X PBS solution at $37^{\circ}C$ for 1 h, the SF film with 3.4% with $60^{\circ}C$ was insoluble. Scanning electron microscopy (SEM) showed that the SF films have solid and smooth surface. Fourier transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC) results showed that the conformation of SF films was influenced by the preparation conditions including SF concentration and casting temperature. In conclusion, SF membrane with transparence and insolubility against exudates could be considered as eardrum patch resources.

Keywords

References

  1. Amoils CP, Jackler RK, Lustig LR (1992) Repair of chronic tympanic membrane perforations using epidermal growth factor. Otolaryngol Head Neck Sitrg 107, 669-683.
  2. Golz A, Netzer A, Joachims HZ, Westerman ST, Gilbert LM (1999) Ventilation tubes and persisting tympanic membrane perforations. Otolaryngol Head Neck Surg 120, 524-527.
  3. Hanawa T, Watanabe A, Tsuchiya T, Ikoma R, Hidaka M, Sugihara M (1995) New oral dosage form for elderly patients. II. Release behavior of benfotiamine from silk fibroin gel. Chem Pharm Bull, 43, 872-876. https://doi.org/10.1248/cpb.43.872
  4. Kalo M, Jackler RK (1996) Repair of chronic tympanic membrane perforations with fibroblast growth factor. Otolaryngol Head Neck Surg 15, 538-547.
  5. Kartush JM (2000) Tympanic membrane patcher: a new device to close tympanic membrane perforations in an office setting. Am J Otol 21, 615-620.
  6. Kim J, Choi J, Kim A, Kim S, Kweon H, Jo Y, Yeo J (2010a) Low molecular weight Silk Fibroin increases alkaline phosphatase and Type I collagen expression in MG63 Cells. BMB Rep 43, 52-56. https://doi.org/10.5483/BMBRep.2010.43.1.052
  7. Kim J, Kim CH, Park CH, Seo J, Kweon H, Kang SW, Lee KG (2010b) Comparison of methods for the repair of acute tympanic membrane perforations: silk patch vs. paper patch. Wound Repair and Regeneration 18, 132-138. https://doi.org/10.1111/j.1524-475X.2009.00565.x
  8. Kweon HY, Ha HC, Um IC, Park YH (2001) Physical properties of silk fibroin/chitosan blend films. J Appl Polym Sci 80, 928-934. https://doi.org/10.1002/app.1172
  9. Kweon HY, Yeo J, Lee K, Lee HC, Na HS, Won YH, Cho CS (2008) Semi-interpenetrating polymer networks composed of silk fibroin and poly(ethylene glycol) for wound dressing. Biomedical Materials 3, 34115. https://doi.org/10.1088/1748-6041/3/3/034115
  10. Magoshi J, Magoshi Y, Nakamur S (1985) Crystallization, liquid crystal, and fiber formation of silk fibroin. J Appl Polym Sci: Appl Polym Symp 41, 187-204.
  11. Minoura N, Tsukada M, Nagura M (1990) Fine structure and oxygen permeability of silk fibroin membrane treated with methanol. Polymer, 31, 265-269. https://doi.org/10.1016/0032-3861(90)90117-H
  12. Minoura N, Aiba S, Gotoh Y, Tsukada M, Imai Y (1995) Attachment and growth of cultured fibroblast cells on silk protein matrices. J Biomed Mater Res 29, 1215-1221. https://doi.org/10.1002/jbm.820291008
  13. Rivas Lacarte MP, Casasin T, Pumarola F, Alonso A (1990) An alternative treatment for the reduction of tympanic membrane perforations: sodium hyaluronate. A double blind study. Acta Otolaryngol 110, 110-114. https://doi.org/10.3109/00016489009122523
  14. Sakabe H, Ito H, Miyamoto T, Noishiki Y, Ha WS (1989) In vivo blood compatibility of regenerated silk fibroin. Sen-I Gakkaishi, 45, 487-490. https://doi.org/10.2115/fiber.45.11_487
  15. Santin M, Motta A, Freddi G, Cannas M (1999) In vitro evaluation of the inflammatory potential of the silk fibron J Biomed Mater Res 46, 382-389. https://doi.org/10.1002/(SICI)1097-4636(19990905)46:3<382::AID-JBM11>3.0.CO;2-R
  16. Sofia S, McCarthy MB, Gronowiza G, Kaplan DL (2001) Functionalized silk-based biomaterials for bone formation. J Biomed Mater Res 54, 139-148. https://doi.org/10.1002/1097-4636(200101)54:1<139::AID-JBM17>3.0.CO;2-7
  17. Tsukada M, Gotoh Y, Nagura M, Minoura N, Kasai N, Freddi G (1994) Structural changes of silk fibroin membranes induced by immersion in methanol aqueous solution. J Polym Sci Polym Phys Ed 32, 961-968. https://doi.org/10.1002/polb.1994.090320519
  18. Yamada H, Igarashi Y, Takasu Y, Saito H, Tsubouchi K (2004) Identification of fibroin-derived peptides enhancing the proliferation of cultured human skin fibroblasts. Biomaterials 25, 467-472. https://doi.org/10.1016/S0142-9612(03)00540-4
  19. Yeo JH, Lee KG, Lee HS, Lee YW, Kim SY (2000) Studies on PVA/chitosan/fibroin blend sponge sheets: preparation and wound healing effects in Rats. Int J Indust Entomol 1, 59-64.

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