• Journal of Microbiology and Biotechnology
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• v.34 no.3
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• pp.681-688
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• 2024

The accurate and rapid detection of methicillin-resistance of Staphylococcus aureus (SA) holds significant clinical importance. However, the methicillin-resistance detection strategies commonly require complicated cell lysis and gene extraction. Herein, we devised a novel colorimetric approach for the sensitive and accurate identification of methicillin-resistance of SA by combining allosteric probe-based target recognition with self-primer elongation-based target recycling. The PBP2a aptamer in the allosteric probe successfully identified the target MRSA, leading to the initiation of self-primer elongation based-cascade signal amplification. The peroxidase-like hemin/G-quadruplex undergo an isothermal autonomous process that effectively catalyzes the oxidation of ABTS2- and produces a distinct blue color, enabling the visual identification of MRSA at low concentrations. The method offers a shorter duration for bacteria cultivation compared to traditional susceptibility testing methods, as well as simplified manual procedures for gene analysis. The overall amplification time for this test is 60 min, and it has a detection limit of 3 CFU/ml. In addition, the approach has exceptional selectivity and reproducibility, demonstrating commendable performance when tested with real samples. Due to its advantages, this colorimetric assay exhibits considerable potential for integration into a sensor kit, thereby offering a viable and convenient alternative for the prompt and on-site detection of MRSA in patients with skin and soft tissue infections.

• Journal of Sensor Science and Technology
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• v.5 no.6
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• pp.25-34
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• 1996

In this paper, we have designed signal conditioning circuitry for piezoresistive pressure sensor. Signal conditioning circuitry consists of voltage reference circuit for sensor driving voltage and instrument amplifier for sensor signal amplification. Signal conditioning circuitry is simulated using HSPICE in a single poly double metal $1.5\;{\mu}m$ BiCMOS technology. Simulation results of band-gap reference circuit showed that temperature coefficient of $21\;ppm/^{\circ}C$ at the temperature range of $0\;{\sim}\;70^{\circ}C$ and PSRR of 80 dB. Simulation results of BiCMOS amplifier showed that dc voltage gain, offset voltage, CMRR, CMR and PSRR are outperformed to CMOS and Bipolar, but power dissipation and noise voltage were more improved in CMOS than BiCMOS and Bipolar. Designed signal conditioning circuitry showed high input impedance, low offset and good CMRR, therefore, it is possible to apply sensor and instrument signal conditioning circuitry.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.5 no.3
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• pp.494-507
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• 2011

In this paper, we propose two schemes to reduce the complexity of fixed-complexity sphere decoder (FSD) algorithm in the ordering and tree-search stages, respectively, while achieving quasi-ML performance. In the ordering stage, we propose a QR-decomposition-based FSD signal ordering based on the zero-forcing criterion (FSD-ZF-SQRD) that requires only a few number of additional complex flops compared to the unsorted QRD. Also, the proposed ordering algorithm is extended using the minimum mean square error (MMSE) criterion to achieve better performance. In the tree-search stage, we introduce a threshold-based complexity reduction approach for the FSD depending on the reliability of the signal with the largest noise amplification. Numerical results show that in 8 ${\times}$ 8 MIMO system, the proposed FSD-ZF-SQRD and FSD-MMSE-SQRD only require 19.5% and 26.3% of the computational efforts required by Hassibi's scheme, respectively. Moreover, a third threshold vector is outlined which can be used for high order modulation schemes. In 4 ${\times}$ 4 MIMO system using 16-QAM and 64-QAM, simulation results show that when the proposed threshold-based approach is employed, FSD requires only 62.86% and 53.67% of its full complexity, respectively.

• Proceedings of the KWS Conference
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• 2005.11a
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• pp.144-146
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• 2005

Dual electromagnetic sensor is used for sensing the weld line. The sensor consists of excitation and two sensing coil wound over the ferro-magnetic core. By using the dual sensor, the effect of noise is minimized. It is based on the generation of eddy currents in the welding plate by passing current through the excitation coil. The sensor can be used to track the butt joints having no gap between them, where a vision based sensor fails to track. Sensor sensitivity depends on the number of coil turns, frequency of excitation, distance of a sensor from the work piece, diameter of core, etc. The whole system consists of a sensor, a signal processing board, a motion controller and a personnel computer (PC). The raw sensor signal is processed using the signal processing board. It consists of amplification, rectification, filtering, averaging, offset adjustment, etc. Based on sensor data, the motion controller adjusts the position of a welding torch.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.5 no.2
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• pp.330-343
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• 2011

In this paper, we propose two schemes to reduce the complexity of fixed-complexity sphere decoder (FSD) algorithm in the ordering and tree-search stages, respectively, while achieving quasi-ML performance. In the ordering stage, we propose a QR-decomposition-based FSD signal ordering based on the zero-forcing criterion (FSD-ZF-SQRD) that requires only a few number of additional complex flops compared to the unsorted QRD. Also, the proposed ordering algorithm is extended using the minimum mean square error (MMSE) criterion to achieve better performance. In the tree-search stage, we introduce a threshold-based complexity reduction approach for the FSD depending on the reliability of the signal with the largest noise amplification. Numerical results show that in $8{\times}8$ MIMO system, the proposed FSD-ZF-SQRD and FSD-MMSE-SQRD only require 19.5% and 26.3% of the computational efforts required by Hassibi’s scheme, respectively. Moreover, a third threshold vector is outlined which can be used for high order modulation schemes. In $4{\times}4$ MIMO system using 16-QAM and 64-QAM, simulation results show that when the proposed threshold-based approach is employed, FSD requires only 62.86% and 53.67% of its full complexity, respectively.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.7 no.4
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• pp.76-85
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• 2008

In this paper, a 800 MHz 1 W cartesian feedback linearized power amplifier is designed and fabricated for TETRA handset application. For amplification of TETRA signal with 200 kHz narrow bandwidth, amplifier linearization performance of more than 30 dBc is improved through the cartesian feedback linearizer at the offset Sequency of ${\pm}25$ kHz. It is clear that the linearization performance is affected by imbalance of gain and phase between I/Q signals and also DC offset. The linearization performance can be maximized by the compensation of those influences. Cartesian feedback is suitable for a liearization technique of narrow band signal with QAM and another modulation signals, as well.

• ETRI Journal
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• v.45 no.5
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• pp.899-909
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• 2023

In mobile communication, the most exploratory technology of fifth generation is massive multiple input multiple output (MIMO). The minimum mean square error and zero forcing based linear detectors are used in multiuser detection for MIMO single-carrier frequency division multiple access (SCFDMA). When the received signal is detected and regularization sequence is joined in the equalization of spectral null amplification, these schemes experience an error performance and the signal detection assesses an inversion of a matrix computation that grows into complexity. Ordered successive interference cancelation (OSIC) detection is considered for MIMO SC-FDMA, which uses a posteriori information to eradicate these problems in a realistic environment. To cancel the interference, sorting is preferred based on signal-to-noise ratio and log-likelihood ratio. The distinctiveness of the methodology is to predict the symbol with the lowest error probability. The proposed work is compared with the existing methods, and simulation results prove that the defined algorithm outperforms conventional detection methods and accomplishes better performance with lower complication.

• Journal of Microbiology and Biotechnology
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• v.33 no.12
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• pp.1692-1697
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• 2023

Staphylococcus aureus integrated with mecA gene, which codes for penicillin-binding protein 2a, is resistant to all penicillins and other beta-lactam antibiotics, resulting in poor treatment expectations in skin and soft tissue infections. The development of a simple, sensitive and portable biosensor for mecA gene analysis in S. aureus is urgently needed. Herein, we propose a dual-toehold-probe (sensing probe)-mediated exonuclease-III (Exo-III)-assisted signal recycling for portable detection of the mecA gene in S. aureus. When the target mecA gene is present, it hybridizes with the sensing probe, initiating Exo III-assisted dual signal recycles, which in turn release numerous "3" sequences. The released "3" sequences initiate catalytic hairpin amplification, resulting in the fixation of a sucrase-labeled H2 probe on the surface of magnetic beads (MBs). After magnet-based enrichment of an MB-H1-H2-sucrase complex and removal of a liquid supernatant containing free sucrase, the complex is then used to catalyze sucrose to glucose, which can be quantitatively detected by a personal glucose meter. With a limit of detection of 4.36 fM for mecA gene, the developed strategy exhibits high sensitivity. In addition, good selectivity and anti-interference capability were also attained with this method, making it promising for antibiotic tolerance analysis at the point-of-care.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.19 no.5
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• pp.547-555
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• 2008

This parer proposed the path mismatch detection and compensation algorithm with closed form for linear amplification with non-linear components(LINC) system implementation. The LINC system has a merit of using the high efficient amplifier by transferring the non-constant envelop signal which is high peak to average signal ratio into constant envelop signal. However, the performance degradation is very sensitive to the path mismatch such as an amplitude mismatch and a phase mismatch. In order to improve the path mismatch, the error detection and compensation method is introduced by the use of four test signals. Since the presented method has the closed form solution, the efficient and fast detection is available. The digital-IF structure of LINC system applied by the proposed error detection and compensation algorithm was implemented. The performance was evaluated with the IEEE 802.16 WiMAX baseband sinal which has 7 MHz channel bandwidth and 16-QAM. The Error Vector Magnitude(EVM) of -37.37 dB was obtained through performance test, which meets performance requirement of -24 dB EVM. As a result, the introduced error detection and compensation method was verified to improve the LINC system performance.

• The Journal of the Acoustical Society of Korea
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• v.35 no.6
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• pp.473-479
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• 2016

We report a highly selective and sensitive 200 MHz Surface Acoustic Wave (SAW) sensor that can detect cyanide ion in aqueous solution using surface immobilized thioester molecules in combination with gold nanoparticles (AuNPs). To construct the sensor device, a monolayer of thioester compound was immobilized on the SAW sensor surface. At the sensor surface, hydrolysis of thioester group by nucleophilic addition of cyanide occurred and the resulting free thiol unit bound to AuNP to form thiol-AuNP conjugate. For the signal enhancement, gold staining signal amplification process was introduced subsequently with gold (III) chloride trihydrate and reducing agent, hydroxylamine hydrochloride. The SAW sensor showed a detection ability of $17.7{\mu}M$ for cyanide in aqueous solution and demonstrated a saturation behavior between the frequency shift and the concentration of cyanide ion. On the other hand, our SAW sensor had no activities for other anions such as fluoride ion, acetate ion and sulfate ion, moreover, no significant interference observed by other anions. Finally, all the experiments were carried out in-house developed sensor and fluidics modules to obtain highly reproducible results.