• Journal of Plant Biotechnology
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• v.2 no.2
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• pp.89-96
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• 2000

For large DNA fragment transformation in dicots and monocots, BIBAC2 vector system was applied to Arabidopsis thaliana and Oryza sativa L. cv. Jinmi as a model plant, respectively. For Arabidopsis, the Th1 gene in T23L3 BAC clone whose size is about 90 kb was used as the target gene source for transformation. Because T23L3 BAC clone was originally constructed in pBelloBAC11, the target gene was reconstructed into BIBAC2. As the results of reconstruction, 476 colonies were survived in selection medium containing 40 mg/L kanamycin. In colony hybridization analysis, 24 out of 476 colonies exhibited positive signals. In the pulsed-field gel electrophoresis analysis, 11 out of 24 positive clones exhibited the band at the location of 90 kb. In Southern hybridization, positive signal band at the location of 90 kb was observed in all 11 transformants. Using these verified clones, Agrobacterium-mediated transformation was applied to Arabidopsis thaliana th1-201 mutant for genetic complementation test. Twelve thousands T$_1$ seeds were harvested, and antibiotic selection test is being analyzed to verify whether these seeds were transformed. for rice, COR356 that contains 150 kb human genomic DNA in a BIBAC2 vector was used as the target gene. As the results of transformation, 151 out of 210 co-cultivated calli were survived in selection medium containing 5 mg/L hygromycin, and 45 out of 151 survived calli were regenerated into plants. Transformation efficiency was 21.6%. Progeny test using 71 seeds is being analyzed now. These results provide the potential that large DNA fragments can be transferred into both dicots and monocot by Agrobacterium-mediate d transformation system.

• Journal of Korea Multimedia Society
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• v.10 no.10
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• pp.1271-1283
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• 2007

To detect defect of FPD(flat panel displays) is very difficult due to uneven illumination on FPD panel image. This paper presents a method to detect various types of defects using the approximated image of the uneven illumination by B-spline. To construct a approximated surface, corresponding to uneven illumination background intensity, while reducing random noises and small defect signal, only the lowest smooth subband is used by wavelet decomposition, resulting in reducing the computation time of taking B-spline approximation and enhancing detection accuracy. The approximated image in lowest LL subband is expanded as the same size as original one by wavelet reconstruction, and the difference between original image and reconstructed one becomes a flat image of compensating the uneven illumination background. A simple binary thresholding is then used to separate the defective regions from the subtracted image. Finally, blob analysis as post-processing is carried out to get rid of false defects. For applying in-line system, the wavelet transform by lifting based fast algorithm is implemented to deal with a huge size data such as film and the processing time is highly reduced.

• Journal of radiological science and technology
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• v.41 no.2
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• pp.123-128
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• 2018

Recently, the use of contrast agents has been increasing as a broader range of tests and dynamic tests have become common due to the development of equipment and imaging techniques such as Multi-Detector CT. However, the side effects of using contrast agents have been reduced by the development of non-ionic contrast agents, but they are still occurring often. The purpose of this study was to propose a method to minimize the side effect of contrast agent by using the amount of contrast agent injected to the brain angiography test to suppress excessive use of contrast agent and analyze the amount of contrast agent. Patients who were prescribed Brain Angiography due to cerebrovascular disease, According to the results of the comparison of the results obtained by dividing into 4 groups of 10ml each according to the amount of contrast medium injected with contrast agent according to the BMI of the patient, BA and SNR were not different between groups, and even if the amount of contrast injection was reduced, there was no problem in the evaluation of CT angiography through 3D reconstruction. This result shows that even if the contrast medium is injected into the blood vessels of the patient first and then the contrast medium is used as the physiological saline solution, the contrast medium is reduced by 40% it can be expected to minimize.

• Journal of the Korean Society of Radiology
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• v.13 no.7
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• pp.941-946
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• 2019

Computed tomography (CT) has been increasing in frequency and indications for use in clinical diagnosis and treatment decisions. Multidetector CT has the advantage of shortening the inspection time and obtaining a high resolution image compared to a single detector CT, but has been pointed out the disadvantage of increasing the radiation exposure. In addition, when the low tube voltage is used to reduce the exposure dose in the CT, noise increases relatively. In the existing method, the method of finding the optimal image quality using the method of adjusting the parameters of the image reconstruction method is not a fundamental measure. In this study, we applied a double-tree complex wavelet algorithm and analyzed the results to maintain the normal signal and remove only noise. Experimental results show that the noise is reduced from 8.53 to 4.51 when using a complex oriented 2D method with 100kVp and 0.5sec rotation time. Through this study, it was possible to remove the noise and reduce the patient dose by using the optimal noise reduction algorithm. The results of this study can be used to reduce the exposure of patients due to the low dose of CT.

• Journal of Magnetics
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• v.17 no.2
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• pp.138-144
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• 2012

3-dimensional magnetic resonance cholangiopancreatography (MRCP) images reconstructed using the maximum intensity projection technique were analyzed qualitatively in patients diagnosed with pancreatobiliary diseases to determine their diagnostic utility. Single shot fast spin echo (SSFSE), fast spin echo (FSE) and 3-dimensional reconstructive images were acquired from 20 patients diagnosed histologically with pancreatobiliary diseases using a 3.0T MR scanner. According to qualitative analysis, the fast spin echo images and 3-dimensional reconstructed images of the hepatic duct, gall bladder and common bile duct had a higher signal to noise ratio (SNR) than the single shot fast spin echo images. Fast spin echo images and 3-dimensional reconstructed images did not show any differences. The contrast to noise ratio of the hepatic duct, gallbladder and common bile duct on the fast spin echo images and 3-dimensional reconstructed images was higher than that of the single shot fast spin echo images. The fast spin echo images and 3-dimensional reconstructed images showed similar quality.

• Korean Journal of Optics and Photonics
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• v.12 no.3
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• pp.219-224
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• 2001

Spectral phase interferometry for direct electric-field reconstruction (SPIDER) was fabricated and used to characterize pulses from a Ti:sapphire oscillator. In the SPIDER apparatus, two replicas of the input pulse were generated with a time delay of 200 fs and were upconverted by use of sum-frequency generation with a strongly chirped pulse using a 8-cm-long SFIO glass block at a 30-11m-thick type II BBO (p-BaBz04) crystal. The resulting interferogram was recorded with a UV-enhanced CCD array in the spectrometer. The spectral phase was retrieved by SPIDER algorithm in combination with independently measured pulse spectrum and the corresponding temporal intensity profile was reconstructed with a duration of 19 fs. As an independent cross-check of the accuracy of the method, we compared the interferometric autocorrelation (lAC) signal calculated from the SPIDER data with a separately measured lAC. The conventional, but unjustified, method of fitting a sechz pulse to the autocorrelation deceivingly yielded a pulse duration of 15 fs. This systematic underestimation of the pulse duration affirms the need for a complete characterization method. From the consideration in this paper, we concluded that the SPIDER could provide an accurate characterization of femtosecond pulses. ulses.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.30 no.8C
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• pp.810-823
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• 2005

The transmission of duplicate packets provides a great loss-resilience without undue time-delay in the video transmission over packet loss networks. But this method generally deteriorates the problem of traffic congestion because of the increased bit-rate required for duplicate transmission. In this paper, we propose an efficient packetization and duplicate transmission of video packets. The proposed method transmits only the video signal with high priority for each video macroblock that is quite small in volume but very important for the reconstruction of the video. The proposed method significantly reduces the required bit-rate for duplicate transmission. An efficient packetization method is also proposed to reduce additional packet overhead which is required for transmitting the duplicate data. The duplicated high priority data of the Previous video slice is transmitted as a Piggyback to the data Packet of the current video slice. It is shown by simulations that the proposed method remarkably improves the packet loss-resilience for video transmission only with small increase of redundant duplicated data for each slice.

• Nuclear Medicine and Molecular Imaging
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• v.42 no.2
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• pp.98-111
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• 2008

This review introduces advances in clinical and pre-clinical single photon emission computed tomography (SPECT) and positron emission tomography (PET) providing noninvasive functional images of biological processes. Development of new collimation techniques such as multi-pinhole and slit-slat collimators permits the improvement of system spatial resolution and sensitivity of SPECT. Application specific SPECT systems using smaller and compact solid-state detector have been customized for myocardial perfusion imaging with higher performance. Combined SPECT/CT providing improved diagnostic and functional capabilities has been introduced. Advances in PET and CT instrumentation have been incorporated in the PET/CT design that provide the metabolic information from PET superimposed on the anatomic information from CT. Improvements in the sensitivity of PET have achieved by the fully 3D acquisition with no septa and the extension of axial field-of-view. With the development of faster scintillation crystals and electronics, time-of-flight (TOF) PET is now commercially available allowing the increase in the signal-to-noise ratio by incorporation of TOF information into the PET reconstruction process. Hybrid PET/SPECT/CT systems has become commercially available for molecular imaging in small animal models. The pre-clinical systems have improved spatial resolution using depth-of-interaction measurement and new collimators. The recent works on solid state detector and dual modality nuclear medicine instrumentations incorporating MRI and optical imagers will also be discussed.

• Journal of Broadcast Engineering
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• v.25 no.4
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• pp.587-597
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• 2020

In this paper, we analyze the normalization that occurs when processing digital hologram and propose an optimized quantization method. In JPEG Pleno, which standardizes the compression of holograms, full complex holograms are defined as complex numbers with 32-bit or 64-bit precision, and the range of values varies greatly depending on the method of hologram generation and object type. Such data with high precision and wide dynamic range are converted to fixed-point or integer numbers with lower precision for signal processing and compression. In addition, in order to reconstruct the hologram to the SLM (spatial light modulator), it is approximated with a precision of a value that can be expressed by the pixels of the SLM. This process can be refereed as a normalization process using quantization. In this paper, we introduce a method for normalizing high precision and wide range hologram using quantization technique and propose an optimized method.

• Steel and Composite Structures
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• v.19 no.3
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• pp.569-583
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• 2015

Recent years, explosive welding structures have been widely used in many engineering fields. The bonding state detection of explosive welding structures is significant to prevent unscheduled failures and even catastrophic accidents. However, this task still faces challenges due to the complexity of the bonding interface. In this paper, a new method called dual-tree complex wavelet transform based permutation entropy (DTCWT-PE) is proposed to detect bonding state of such structures. Benefiting from the complex analytical wavelet function, the dual-tree complex wavelet transform (DTCWT) has better shift invariance and reduced spectral aliasing compared with the traditional wavelet transform. All those characters are good for characterizing the vibration response signals. Furthermore, as a statistical measure, permutation entropy (PE) quantifies the complexity of non-stationary signals through phase space reconstruction, and thus it can be used as a viable tool to detect the change of bonding state. In order to more accurate identification and detection of bonding state, PE values derived from DTCWT coefficients are proposed to extract the state information from the vibration response signal of explosive welding structure, and then the extracted PE values serve as input vectors of support vector machine (SVM) to identify the bonding state of the structure. The experiments on bonding state detection of explosive welding pipes are presented to illustrate the feasibility and effectiveness of the proposed method.