• Fisheries and Aquatic Sciences
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• 제19권4호
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• pp.16.1-16.8
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• 2016

To culture germline stem cells in vitro, establishment of the cell lines that can be used as the feeder cells is a prerequisite. In this study, we tried to establish gonad-derived cell lines in Siberian sturgeon (Acipenser baerii). Five 1-year-old A. baerii were used as a donor of gonad tissues, and gonad-dissociated cells were cultured in vitro. Subsequently, determination of growth conditions, long-term culture, characterization, and cryopreservation of the cell lines were also conducted. Five gonad-derived cell lines were stably established and cultured continuously over at least the 73th passage and 402 culture days under the media containing 20 % fetal bovine serum at $28^{\circ}C$. All cell lines consisted of two main cell types based on morphology even if the ratio of the two cell types was different depending on cell lines. Despite long-term culture, all cell lines maintained diploid DNA contents and expression of several genes that are known to express in the A. baerii gonad. After freezing and thawing of the cell lines, post-thaw cell viabilities between 57.6 and 92.9 % depending on cell lines were indentified, suggesting that stable cryopreservation is possible. The results and the cell lines established in this study will contribute to the development of an in vitro system for A. baerii germline stem cell culture.

• 한국항해항만학회지
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• 제31권6호
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• pp.555-562
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• 2007

겨울철 한반도와 부근 해역은 대륙에서 발달하는 시베리아고기압과 해양에서 발달하는 알류샨저기압으로 형성되는 전형적인 서고동저형의 기압배치의 영향을 많이 받는다. 이 기압배치의 영향으로 우리나라와 주변 해역, 특히 서해에서는 강한 서풍 내지 북서풍의 계절풍과 강한 바람에 의한 높은 파랑, 폭설 등을 동반하는 악기상이 빈번히 발생한다. 이 연구에서는 겨울철에 서해에서 활동하는 국가어업지도선의 안전한 피항지를 선정하기 위하여 11년간 (1994-2004년)의 지상일기도를 분석하여 온대저기압의 주요 이동경로를 구분하였다. 그리고 온대저기압의 이동경로별로 섬들의 지형적 특성, 수심, 저질의 상대, 조류의 영향 및 어망의 분포 등을 조사하고, 그 결과를 이용하여 서해에서 활동하는 국가어업지도선의 안전한 피항지를 선정하였다.

• Animal cells and systems
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• 제11권2호
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• pp.165-168
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• 2007

The nucleotide sequences of a male-specific marker sex determining region Y (SRY) gene and a mitochondrial cytochrome B (CYTB) gene were characterized and analyzed to establish a molecular method for identification of species and sex of Korean roe deer (Capreolus pygargus tianschanicus). Similarity search result of SRY sequences showed very similar result to those reported in Moose (Alces alces) and Reindeer (Rangifer tarandus), both of which had 95.9% similarity in identity. CYTB genes were very similar to those reported in Siberian roe deer (C. pygargus pygargus) which had 98.6% similarity and not to European roe deer (C. capreolus), suggesting that the DNA samples tested were of Siberian roe deer lineage. Polymerase chain reaction (PCR)-based sex typing successfully discriminated between carcasses of male and female roe deer. Males had SRY band on agarose gels and females did not. The result of this molecular sex typing provided similar information with that obtained by genital organ observation. Therefore, this molecular method using male specific marker SRY and mitochondrial CYTB genes would be very useful for identification of the species and sex of the carcass remains of roe deer.

• 대기
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• 제20권3호
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• pp.307-318
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• 2010

This study investigates the generation and movement of low-concentration $CO_2$ observed in Gosan during summer from 2002 to 2006. For analysis, additional $CO_2$ data in Anmyeondo, Ryori, Barrow, and Minamitorishima as well as NOAA/ESRL daily global $CO_2$ fields, background trajectories data, and 850 hPa wind fields are also used. Based on analyses using various observed data, we classified three types of low-concentration $CO_2$ in Gosan according to its origin: i) the origin of the Siberian continental, in which the consumption of $CO_2$ is active due to photosynthesis from broad forests, ii) the origin of Okhotsh and Artic seas, in which the low-concentration $CO_2$ is dominant due to high primary productivity by a plankton bloom, and iii) the origin of the Northwestern Pacific which is related to the entry of air mass from high latitudes. These results show that the low-concentration $CO_2$ observed in Gosan during summer is not originated from the Pacific oceans as known in previous studies, but originated from high latitude regions such as the Siberian continental and the Okhotsh and Artic seas.

• 한국수산과학회지
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• 제30권6호
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• pp.974-983
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• 1997

The Okhotsk Sea is unique natural object with climatic peculiarities. The climate of the Okhotsk Sea results from the general distribution of solar radiation during a year, and the characteristics of the atmospheric circulation that varies through a year: In cold half year the main pressure formations are Siberian high and Aleutian low. Asian low centered on Afghanistan dominates over the Asian continent in summer. The North-Pacific sea surface is under effect of permanent North Pacific high. The changes in their position from year to year are very significant. The anticyclonic activity over the Far Eastern Seas is one of the main factors for the formation of weather anomalies over the adjacent territories. The analysis of summer weather characteristics over the coast of Okhotsk and East Sea using the data obtained from Hydrometeorological stations during $1949\~1990$ showed that, to a great extent, distribution of the air temperature depends on thermal state of the Okhotsk Sea and atmospheric circulation over it. We show some relations between weather characteristics and the intensity of atmospheric action center for the North Pacific high in summer when its ridge propagates to Okhotsk Sea. Correlation coefficients between air pressure over the Okhotsk Sea and air temperature for the coastal areas reach up to 0.7. Analysis of the spatial-temporal distribution of main meteorological values over the Okhotsk Sea such as air pressure, and air temperature are also performed.

• Nuclear Engineering and Technology
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• 제52권11호
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• pp.2460-2470
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• 2020

To study the thermophysical and neutronic properties of thorium-plutonium fuel, a conceptual design of a hybrid facility consisting of a subcritical Th-Pu reactor core and a source of additional D-D neutrons that places on the axis of the core is proposed. The source of such neutrons is a column of high-temperature plasma held in a long magnetic trap for D-D fusionreactions. This article presents computer simulation results of generation of thermonuclear neutrons in the plasma, facility neutronic properties and the evolution of a fuel nuclide composition in the reactor core. Simulations were performed for an axis-symmetric radially profiled reactor core consisting of zones with various nuclear fuel composition. Such reactor core containing a continuously operating stationary D-D neutron source with a yield intensity of Y = 2 × 10¹⁶ neutrons per second can operate as a nuclear hybrid system at its effective coefficient of neutron multiplication 0.95-0.99. Options are proposed for optimizing plasma parameters to increase the neutron yield in order to compensate the effective multiplication factor decreasing and plant power in a long operating cycle (3000-day duration). The obtained simulation results demonstrate the possibility of organizing the stable operation of the proposed hybrid 'fusion-fission' facility.

• 한국임상수의학회지
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• 제29권2호
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• pp.165-168
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• 2012

14년 령의 중성화 하지 않은 수컷 Siberian Husky는 1개월 전부터 혈뇨, 기면, 식욕부진을 보였다. 신체검사상 복부 팽만과 통증을 확인할 수 있었다. 혈액 및 혈청 화학 검사상 빈혈과 질소혈증을 확인할 수 있었다. 방사선 검사 상 신장의 비대를 확인할 수 있었으며 초음파 검사 상 피수질의 경계가 불분명하며 피질의 에코가 증가한 신장을 확인할 수 있었다. 뇨검사 상에서는 혈뇨와 단백뇨를 확인할 수 있었으며 초기의 감별 진단으로는 신부전, 방광염, 신우신염과 종양을 생각했다. 환자의 상태는 내원 후 계속적으로 악화되었으며 폐사했다. 부검 상에서 복강 내 출혈과 양측 신장의 종양을 확인할 수 있었다. 조직검사 상에서 혈관육종을 진단하였다. 본 증례에서는 개에서 비특이적으로 발생하는 양측성의 신장원성 혈관육종에 대해서 다룬다.

• 대한지리학회지
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• 제40권3호
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• pp.285-295
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• 2005

본 연구에서는 우리나라 19개 지점의 일평균기온을 분석하여 꽃샘추위 발생 일수의 분포와 변화 경향을 파악하고자 하였다. 꽃샘추위 발생일은 일평균기온과 일평균기온 평년값(1971-2000)의 편차를 계산하여 일평균기온이 평년값보다 낮은 상위 $10\%$에 해당하거나 당일과 2일전과의 평균기온 차에서 당일의 평균기온이 낮은 상위 $10\%$에 해당하는 날로 정의하였다. 꽃샘추위 발생일은 서해안이 동해안보다 많고, 위도가 높아질수록 많다. 꽃샘추위 발생일의 순별 분포 유형은 기압계 및 지형과 밀접한 관련이 있어 동해안과 서해안 지역, 도서 지역으로 구별된다. 꽃샘추위의 발생 일수는 한반도가 시베리아 고기압의 영향을 받는 빈도 및 강도와 관련이 있으며 최근 대부분 지역에서 감소하는 경향이다. 그러나 울릉도와 동해안 지역에서는 꽃샘추위 발생 일수 변화가 거의 없어 꽃샘추위 발생 원인이 다르다.

• Animal cells and systems
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• 제7권2호
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• pp.139-144
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• 2003

We analyzed seven Y chromosome binary markers (YAP, RPS4Y_711,\;M9,\;M175,\;LINE1,\;SRY_+465$ and 47z) in samples from a total of 254 males from Koreans and tow Mongolian ethnic groups (Buryat and Khalkh) to study the genetic relationship among these populations. We found eight distinct Y haplogroups constructed from the seven binary markers. Haplogroup DE-YAP was present at extremely low frequencies (∼2%) in the Korean and Mongolian populations. This result is consistent with earlier reports that showed the YAP+ chromosomes to be highly polymorphic only in populations from Japan and Tibet in east Asia. The observed high frequency of haplogroup $C-RPS4Y_711$ in the Mongolian populations (∼40%) is concordant with recent findings, showing that the $RPS4Y_711$-T chromosomes were distributed at high frequencies in Siberian and Mongolian populations compared with most other populations from east Asia. Thus, the relatively moderate frequency of haplogroup $C-RPS4Y_711$ in Korean (∼15%) can be seen as genetic evidence for probable interaction with Mongolian and/or Siberian populations. In contrast, the majority (∼75%) of modern Koreans studied here had high frequencies of Y chromosome lineages of haplogroup O-M175 and additional haplogroupts that define sublineage of O-M175, which are most likely related with modern populations in China. In conclusion, our data on the Y chromosome haplogroup distribution may provide evidence for interaction between Korean and Mongolian populations, but Korean tend to be much more related with those from southern-to-northern populations of China than to Mongolians in east Asia.

• 한국항만경제학회지
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• 제27권4호
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• pp.145-164
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• 2011

최근 동북아시아지역의 교역규모가 급성장함에 따라 동북아시아 경제권이 세계경제에서 점유하는 비중이 점차 늘어나고 있다. 이러한 대륙 국가들과의 주요 연결로서 가장 큰 구심점 역할을 하고 있는 것이 Trans Siberian Railway(TSR) 등이라고 할 수 있다. TSR 이용의 경우 국내 도로운송과 부산항과 극동 러시아 항만간 해상운송, 대륙횡단철도, 최종목적지 도착이후의 도로운송을 포함하고 있어 항공운송을 제외한 복합운송의 전 영역에 걸쳐 있으며 최근 들어 국내 철도 화물운송을 활성화시켜 대륙횡단철도와 연계하여 운영하는 것에 대한 방안도 활발하게 논의되고 있다. 따라서 본 연구는 첫째, 러시아 및 중앙아시아향(向) 화물 운송의 선봉장 역할을 하고 있는 우리나라 TSR Service Operator들의 특징 및 역할을 파악하고 둘째, TSR Service Operator의 SWOT 분석으로 대변되는 대내 외적인 환경 분석과 우리나라 TSR Service Operator의 현황을 분석하고 셋째, 이를 바탕으로 우리나라 TSR Service Operator가 지속적인 경쟁우위를 확보하고 유지할 수 있는 발전 방향을 제시하는데 그 목적이 있다.