Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Identification of Species and Sex of Korean Roe Deer (Capreolus pygargus tianschanicus) Using SRY and CYTB Genes

  • Han, Sang-Hyun (National Institute of Subtropical Agriculture, Rural Development Adminstration) ;
  • Cho, In-Cheol (National Institute of Subtropical Agriculture, Rural Development Adminstration) ;
  • Lee, Sung-Soo (National Institute of Subtropical Agriculture, Rural Development Adminstration) ;
  • Tandang, Leoncia (National Institute of Subtropical Agriculture, Rural Development Adminstration) ;
  • Lee, Hang (Conservation Genome Resource Bank for Korean Wildlife (CGRB), College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University) ;
  • Oh, Hong-Shik (Department of Science Education, Cheju National University) ;
  • Kim, Byoung-Soo (Department of Life Science, Cheju National University) ;
  • Oh, Moon-You (Department of Life Science, Cheju National University)
  • Published : 2007.12.31
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Abstract

The nucleotide sequences of a male-specific marker sex determining region Y (SRY) gene and a mitochondrial cytochrome B (CYTB) gene were characterized and analyzed to establish a molecular method for identification of species and sex of Korean roe deer (Capreolus pygargus tianschanicus). Similarity search result of SRY sequences showed very similar result to those reported in Moose (Alces alces) and Reindeer (Rangifer tarandus), both of which had 95.9% similarity in identity. CYTB genes were very similar to those reported in Siberian roe deer (C. pygargus pygargus) which had 98.6% similarity and not to European roe deer (C. capreolus), suggesting that the DNA samples tested were of Siberian roe deer lineage. Polymerase chain reaction (PCR)-based sex typing successfully discriminated between carcasses of male and female roe deer. Males had SRY band on agarose gels and females did not. The result of this molecular sex typing provided similar information with that obtained by genital organ observation. Therefore, this molecular method using male specific marker SRY and mitochondrial CYTB genes would be very useful for identification of the species and sex of the carcass remains of roe deer.

Keywords

References

  1. An J, Lee MY, Min MS, Lee MH and Lee H (2007) A molecular genetic approach for species identification of mammals and sex determination of birds in a forensic case of poaching from South Korea. Forensic Sci Int 167: 59-61 https://doi.org/10.1016/j.forsciint.2005.12.031
  2. Anderson GB (1987) Identification of embryonic sex detection of H-Y antigen. Theriogenology 27: 81-97 https://doi.org/10.1016/0093-691X(87)90072-0
  3. Angelo M and Moreira M (2002) SRY evolution in Cebidae (Platyrrhini: Primates). J Mol Evol 55: 92-103 https://doi.org/10.1007/s00239-001-2308-7
  4. Birren B, Green ED, Klapholz S, Myers RM and Roskams J 1997. Genome analysis: A laboratory manual. Vol. 1 Analyzing DNA, Cold Spring Harbor Laboratory Press, New York, pp 6-16
  5. Cao X, Jiang H and Zhang X (2005) Polymorphic karyotypes and sex chromosomes in the tufted deer (Elaphodus cephalophus): cytogenetic studies and analyses of sex chromosome-linked genes. Cytogenet Genome Res 109: 512-518 https://doi.org/10.1159/000084212
  6. Chikuni K, Mori Y, Tabata T, Saito M, Monma M and Kosugiyama M (1995) Molecular phylogeny based on the kappa-casein and cytochrome b sequences in the mammalian suborder Ruminantia. J Mol Evol 41: 859-866
  7. Dreesen CJFM, Dumoulin JCM, Evers JLH, Geraedts JPM and Pieters MHEC (1995) Multiplex polymerase chain reaction for sex determination of single mouse blastomeres. Mol Human Reprod 10: 743-748 https://doi.org/10.1093/molehr/gah094
  8. Gardner RL and Edwards RG (1968) Control of the sex ratio at full term in rabbit by transferring sexed blastocysts. Nature 218: 346-349 https://doi.org/10.1038/218346a0
  9. Groves CP and Grubb P (1987) Relationships of living deer. In: Wemmer CM (ed), Biology and Management of the Cervidae, Smithsonian Institution Press, Washington, pp 21-59
  10. Grubb P (1993) Order Artiodactyla. In: Wilson DE and Reeder DM (eds), Mammal Species of the World, A Taxonomic and Geographic Reference, Smithsonian Institution Press, Washington, pp 377-414
  11. Koh HS, Yang Bo, Yoo HS and Chun TY (2000) Diversity of mitochondrial cytochrome b gene in roe deer (Caprelous pygargus tianschaniucs Satunin) from Jejudo Island, Korea. Korean J Syst Zool 16: 169-176
  12. Koopman P, Gubbay J, Vivian N, Goodfellow P and Lovell-Badge R (1991) Male development of chromosomally female mice transgenic for SRY. Nature 351: 117-121 https://doi.org/10.1038/351117a0
  13. Lee JH, Park JR, Lee SH, Park CS and Jin DI (2004) Sexing using single blastomere derived from IVF bovine embryos by fluorescence in situ hybridization (FISH). Theriogenology 62: 1452-1458 https://doi.org/10.1016/j.theriogenology.2004.02.012
  14. Pfeiffer I and Brenig B (2005) X- and Y-chromosome specific variants of the amelogenin gene allow sex determination in sheep (Ovis aries) and European red deer (Cervus elaphus). BMC Genet 6: 16
  15. Phua ACY, Abdullah RB and Monamed Z (2003) A PCR-based sex determination method for possible application in caprine gender selection by simultaneous amplification of the Sry and Aml-X genes. J Reprod Dev 49: 307-311 https://doi.org/10.1262/jrd.49.307
  16. Quilter CR, Blott SC, Mileham AJ, Affara NA, Sargent CA and Griffin DK (2002) A mapping and evolutionary study of porcine sex chromosome genes. Mamm Genome 13: 588-594 https://doi.org/10.1007/s00335-002-3026-1
  17. Randi E, Pierpaoli M and Danilkin A (1998) Mitochondrial DNA polymorphism in populations of Siberian and European roe deer (Capreolus pygargus and C. capreolus). Heredity 80: 429-347 https://doi.org/10.1046/j.1365-2540.1998.00318.x
  18. Shea BF (1999) Determining the sex of bovine embryos using polymerase chain reaction results: a six-year retrospective study. Theriogenology 51: 841-854 https://doi.org/10.1016/S0093-691X(99)00030-8
  19. Sinclair AH, Berta P, Palmer MS, Hawkins JR, Griffiths BL, Smith MJ, Foster JW, Frischauf A-M, Lovell-Badge Rand Goodfellow PN (1990) A gene from the human sexdetermining region Y encodes a protein with a homology to a conserved DNA-binding motif. Nature 346: 240-244 https://doi.org/10.1038/346240a0
  20. Takahashi M, Masuda R, Uno H, Yokoyama M, Suzuki M, YoshidaMC and Ohtaishi N (1998) Sexing of carcass remains of the Sika deer (Cervus nippon) using PCR amplification of the Sry gene. J Vet Med Sci 60: 713-716 https://doi.org/10.1292/jvms.60.713
  21. Wilson P J and White BN (1998) Sex identification of elk (Cervus elaphus canadensis), moose (Alces alces), and white-tailed deer (Odocoileus virginianus) using the polymerase chain reaction. J Forensic Sci 43: 477-482
  22. Yamamoto K, Tsubota T, Komatsu T, Katayama A, Murase T, Kita I and Kudo T (2002) Sex identification of Japanese black bear, Ursus thibetianus japonicus, by PCR based on amelogenin gene. J Vet Med Sci 64: 505-508 https://doi.org/10.1292/jvms.64.505
  23. Yamauchi K, Hamasaki S, Miyazaki K, Kikusui T, Takeuchi Y and Mori Y (2000) Sex determination based on fecal DNA analysis of the amelogenin gene in sika deer (Cervus nippon). J Vet Med Sci 62: 669-671 https://doi.org/10.1292/jvms.62.669