• Title/Summary/Keyword: Shotgun

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Whole-Genome Sequencing by the random shotgun approach (Random shotgun 방법을 이용한 생물체의 염기서열 분석)

  • Jung, Chol-Hee;Yoon, Kyong-Oh;Park, Hyun-Seok;Choi, Jin-Young
    • Proceedings of the Korea Information Processing Society Conference
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    • 2000.10a
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    • pp.207-210
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    • 2000
  • 지금까지 인간이나 다른 생물체의 전체 유전체 염기서열을 밝혀내는 작업은 크게 세가지 방법으로 진행되었다. Clone-by-clone approach, sequence tagged connector approach, random shotgun approach(1)가 그것인데 마지막의 random shotgun approach는 fragment assembly problem을 비롯한 여러 가지 전산학적인 문제들을 수반한다. 이 논문은 저자들의 국내 최초로 미생물체의 전체 염기서열을 random shotgun approach를 이용하여 밝혀낸 경험을 바탕으로 그에 따르는 문제인 fragment assembly problem에 대해 소개하고 그에 수반되는 몇 가지 전산학적인 문제와 몇 가지 해결책에 대해 설명하려 한다.

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The Study on Solid Propellant Deflagrabillity by Shotgun & RQ Bomb Test (Shotgun & RQ Bomb시험에 의한 추진제 폭연 특성)

  • 유지창;김창기;이경주
    • Journal of the Korean Society of Propulsion Engineers
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    • v.6 no.3
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    • pp.9-17
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    • 2002
  • This Study is to investigate 8 composite propellants including Butacene and ${Bi_2}{O_3}$ by Shotgun/RQ Bomb test. Burning rate and mechanical properity are known to be major factors in determining the deflagrability of propellant. Propellant including over 5.5% Butacene(Ferrocene grafted HTPB) burned out over 135 m/s of impact velocity during Shotgun/RQ Bomb test. It was blown that Butacene was very sensitive material under high velocity impact. In the test results, propellants under 25mm/s in burning rate at 1500 psia could meet the requirements for IM of UN Test Series 7c(ii). Propellant deflagrabillity depends on burning rate at performance in the results of the present.

A Study on the Efficiency & Limitation of 3D Animation Production Management Using Production Management Tool - Focusing on Shotgun Software & Ftrack (3D 애니메이션 제작 관리를 위한 제작관리도구(Tool)의 효율성 및 한계 - 샷건(Shotgun)과 Ftrack(에프트랙)을 중심으로)

  • Lee, Esther Kkotsongyi
    • Cartoon and Animation Studies
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    • s.49
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    • pp.1-23
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    • 2017
  • 3D animation production has had a pivotal position in current animation industry and the necessity of professional management tool for 3D animation production has claimed due to its sophisticated pipeline from advance of technology and global production partnership trend. Shotgun and Ftrack are providing the most appropriate management toolset for 3D animation management among the extant management tools and the efficiency of Shotgun & Ftrack is identified compared with the traditional document oriented management style. The biggest strength of production management using Shotgun is that all of the production staff can directly participate in the communication on the tools therefore they can share the information on Shotgun & Ftrack in real time without constraint of time and location. Moreover, all the process of the production and the history of the discussion on certain production issues are systematically accrue on the tool so that the production history can be easily tracked. Finally, the production management using tools contributes collecting and analysing the production information for the production management team in studios. However, Shotgun & Ftrack has metadata based retrieval method which cost huge amount of effort by human's manual annotation and it also has the limitation of accuracy. In addition, the fact that studios has to have technical professionals first in order to institute the tools into their studios is the actual difficulty of Korean studios when they want to use management tools for their project. Thus, this paper suggests adopting the content-based retrieval system on the tools and tools' expanded technical service for the studios as the solution of the identified issues.

Design and Implementation of the genome-level fragment assembly system, Mater (Fragment Assembly를 위한 시스템의 설계 및 구현)

  • 김명선;정철희;박현석
    • Proceedings of the Korean Information Science Society Conference
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    • 2001.04a
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    • pp.751-753
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    • 2001
  • 지금까지 인간이나 다른 생물체의 전체 유전체 염기서열을 밝혀내는 작업은 크게 세가지 방법으로 진행되었다. Clone-by-clone approach, sequence tagged connector approach, random shotgun approach[1]가 그것인데 마지막의 random shotgun approach는 fragment assembly problem을 비롯한 여러 가지 전산학적인 문제들을 수반한다. 미생물체의 전체 염기서열을 random shotgun approach를 이용하여 밝혀낼 때 몇 가지 전산학적인 문제가 테크닉이 필요하며 그 중에서도 서열간의 forward, reverse의 mating 정보를 이용하는 것이 중요하다. 본 논문은 이러한 mating 작업을 한 눈에 볼 수 있게 하는 소프트웨어 페키지 “Mater”에 대해 소개하고자 하며 그 의미에 대해 논하고자 한다.

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Stochastic Modeling and Concurrent Simulation of the Game of Golf

  • Yoon, Sung-Roh;Lee, Se-Il;Oh, Seong-Jun
    • ETRI Journal
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    • v.31 no.6
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    • pp.809-811
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    • 2009
  • We propose a novel simulation method for modeling the game of golf using SystemC, a system description language that allows modeling of a concurrent system's behavior. Utilizing the proposed simulator, we compare different outing formats of golf, namely, regular and shotgun, in terms of playing time. Our simulation results reveal that the shotgun format can take longer than the regular format if the number of groups in a golf course exceeds 33 for the scenario we tested, confirming the belief that the shotgun format can take longer than the regular format. We also justify our simulation by comparing the simulation and analytical results.

Shotgun/RQ Bomb 시험에 의한 추진제 폭연 특성

  • 유지창;김창기;류백능
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2000.11a
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    • pp.29-29
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    • 2000
  • 추진제의 폭연 특성을 실험실 규모로 측정하는 시험방법으로 NAWC에서 개발한 BIC (Ballistic Impact Chamber) 시험과 SNPE에서 개발한 Shotgun/RQ Bomb 시험이 있다. SNPE에서는 화약과 추진제가 UNO 위험등급 분류체계에서 1.6급수(우연한 개시나 반응의 전환 가능성이 거의 없는 매우 둔감한 물질)로 분류되는 기준을 UN Test Series 7c(ii)에 탄속 150m/s에서 dP/dt가 15MPa 이내로 규정하고 있다.(중략)

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FASIM: Fragments Assembly Simulation using Biased-Sampling Model and Assembly Simulation for Microbial Genome Shotgun Sequencing

  • Hur Cheol-Goo;Kim Sunny;Kim Chang-Hoon;Yoon Sung-Ho;In Yong-Ho;Kim Cheol-Min;Cho Hwan-Gue
    • Journal of Microbiology and Biotechnology
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    • v.16 no.5
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    • pp.683-688
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    • 2006
  • We have developed a program for generating shotgun data sets from known genome sequences. Generation of synthetic data sets by computer program is a useful alternative to real data to which students and researchers have limited access. Uniformly-distributed-sampling clones that were adopted by previous programs cannot account for the real situation where sampled reads tend to come from particular regions of the target genome. To reflect such situation, a probabilistic model for biased sampling distribution was developed by using an experimental data set derived from a microbial genome project. Among the experimental parameters tested (varied fragment or read lengths, chimerism, and sequencing error), the extent of sequencing error was the most critical factor that hampered sequence assembly. We propose that an optimum sequencing strategy employing different insert lengths and redundancy can be established by performing a variety of simulations.

Shotgun Phage Display of Lactobacillus casei BL23 Against Collagen and Fibronectin

  • Munoz-Provencio, Diego;Monedero, Vicente
    • Journal of Microbiology and Biotechnology
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    • v.21 no.2
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    • pp.197-203
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    • 2011
  • Lactobacilli are normal constituents of the intestinal microbiota, and some strains show the capacity to bind to extracellular matrix proteins and components of the mucosal layer, which represents an adaptation to persist in this niche. A shotgun phage-display library of Lactobacillus casei BL23 was constructed and screened for peptides able to bind to fibronectin and collagen. Clones showing binding to these proteins were isolated, which encoded overlapping fragments of a putative transcriptional regulator (LCABL_29260), a hypothetical protein exclusively found in the L. casei/rhamnosus group (LCABL_01820), and a putative phage-related endolysin (LCABL_13470). The construction of different glutathione S-transferase (GST) fusions confirmed the binding activity and demonstrated that the three identified proteins could interact with fibronectin, fibrinogen, and collagen. The results illustrate the utility of phage display for the isolation of putative adhesins in lactobacilli. However, it remains to be determined whether the primary function of these proteins actually is adhesion to mucosal surfaces.

A Simple Java Sequence Alignment Editing Tool for Resolving Complex Repeat Regions

  • Ham, Seong-Il;Lee, Kyung-Eun;Park, Hyun-Seok
    • Genomics & Informatics
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    • v.7 no.1
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    • pp.46-48
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    • 2009
  • Finishing is the most time-consuming step in sequencing, and many genome projects are left unfinished due to complex repeat regions. Here, we have developed BACContigEditor, a prototype shotgun sequence finishing tool. It is essentially an editor that visualizes assemblies of shotgun sequence fragment reads as gapped multiple alignments. The program offers some flexibility that is needed to rapidly resolve complex regions within a working session. The sole purpose of the release is to promote collaborative creation of extensible software for fragment assembly editors, foster collaborative development, and reduce barriers to initial tool development effort. We describe our software architecture and identify current challenges. The program is available under an Open Source license.