• BMB Reports
• /
• v.53 no.7
• /
• pp.341-348
• /
• 2020

The targeted nuclease clustered, regularly interspaced short palindromic repeats/CRISPR-associated proteins (CRISPR/Cas) system has recently emerged as a prominent gene manipulation method. Because of its ease in programming targeted DNA/protein binding through RNA in a vast range of organisms, this prokaryotic defense system is a versatile tool with many applications in the research field as well as high potential in agricultural and clinical improvements. This review will present a brief history that led to its discovery and adaptation. We also present some of its restrictions, and modifications that have been performed to overcome such restrictions, focusing specifically on the most common CRISPR/Cas9 mediated non-homologous end joint repair.

• Korean Journal of Breeding Science
• /
• v.41 no.4
• /
• pp.635-639
• /
• 2009

A new peanut variety "Pungan"(Arachis hypogaea ssp. hypogaea L.) was developed at the Department of Functional Crop, NICS(National Institute of Crop Science), in Milyang in 2008. It was developed from the cross between the high-yielding cultivar "Saedl" and the very short stem cultivar "Satonoka". "Pungan", a Virginia palnt type, has 20 branches with long ellipseshaped large grains. The 100 grain weight was 88 g, 7 g heavier than the check variety Daekwang. This variety is more resistant to late leaf spot and web blotch compared to Daekwang, a check variety. Moreover, it is lodging resistant owing to its short stem. "Pungsan" outyielded the check variety by 16% with 4.67 MT/ha in regional yield trials.

• Korean Journal of Breeding Science
• /
• v.40 no.1
• /
• pp.63-67
• /
• 2008

A new peanut cultivar "Jopyeong" (Arachis hypogaea ssp. hypogaea L.) was developed at the Yeongnam Agricultural Research Institute, NICS, in Milyang in 2006. It was developed from the cross between the very short stem cultivar "CUP brittle" and the high-yielding cultivar "Daekwang". "Jopyeong" which is Virginia plant type has 23 branch number per plant with early maturing and ellipse-shaped large kernel. Each pod has two grains with brown testa and 100 seed weight was 87g in the regional yield trials (RYT). This variety also showed more resistant to late leaf spots compared with check one. Especially it has resistance to lodging owing to short stem and erect plant type. In the regional yield trials "Jopyeong" was out-yielded than check variety by 11% with 8.37 ton/ha for fresh pod and by 4% with 3.95 ton/ha for grain.

• Korean Journal of Plant Resources
• /
• v.25 no.6
• /
• pp.745-752
• /
• 2012

Compared to wide ranges of genetic variation of natural populations, very limited Miscanthus cultivar has been released. This study was the first report on the development of Miscanthus cultivar by means of radiation breeding. Seeds of M. sinensis were initially exposed to gamma rays of 250 Gy for 24 h, generated from a $^{60}Co$ gamma-irradiator. The irradiated seeds were sown and then the highly tiller-producing mutants were selected for this study. Biomass-related parameters including tiller number, plant height, stem diameter, and leaf number were measured. Ploidy level and internal transcribed spacer (ITS) were investigated to characterize the mutants compared to wild type (WT) Miscanthus. Plant height and tiller number were negatively related, where multi-tillering mutants were relatively short after 4 month growth. However stem diameter and leaf number were greater in mutants. All the materials used in this study were diploid, implying that the mutants with greater tiller numbers and stem diameter were not likely related to polyploidization. Based on the sequence of ITS regions, the mutants demonstrated base changes from the gamma irradiation where G+C content (%) was decreased in the ITS1, but increased in ITS2 when compared to WT sequence. ITS2 region was more variable than in ITS1 in the mutants, which collectively allows identification of the mutants from WT. Those mutants having enhanced tillers and allelic variations might be used as breeding materials for enhanced biomass-producing Miscanthus cultivars.

• Biomedical Science Letters
• /
• v.23 no.2
• /
• pp.49-56
• /
• 2017

Fibroblast growth factor (FGF)-2 is one of the most effective growth factors to increase the growth rate of mesenchymal stem cells (MSCs). Previously, we reported that low dose of FGF-2 (1 ng/ml) induced proliferation of bone marrow-derived mesenchymal stem cells (BMSCs) through AKT and ERK activation resulting in reduction of autophagy and senescence, but not at a high dose. In this study, we investigated the effects of high dose FGF-2 (10 ng/ml) on proliferation, autophagy and senescence of BMSCs for long term cultures (i.e., 2 months). FGF-2 increased the growth rate of BMSCs in a dose dependent manner for a short term (3 days), while during long term cultures (2 months), population doubling time was increased and accumulated cell number was lower than control in BMSCs when cultured with 10 ng/ml of FGF-2. 10 ng/ml of FGF-2 induced immediate de-phosphorylation of ERK1/2, expression of LC3-II, and increase of senescence associated ${\beta}$-galactosidase (SA-${\beta}$-Gal, senescence marker) expression. In conclusion, we showed that 10 ng/ml of FGF-2 was inadequate for ex vivo expansion of BMSCs because 10 ng/ml of FGF-2 induced growth retardation via ERK1/2 de-phosphorylation and induction of autophagy and senescence in BMSCs.

• Journal of Veterinary Clinics
• /
• v.28 no.1
• /
• pp.63-70
• /
• 2011

To consider the iliac fossa as the vascular anastomosis site of kidney transplantation for the short-term study of acute rejection in pigs. Twelve domestic pigs weighing 39~48 kg underwent heterotopic renal allgraft transplantation. The experimental animals were divided into 2 groups in terms of renal vascular anastomosis site; the external iliac artery and vein were used in iliac fossa model (n = 6), the abdominal aorta and the caudal vena cava inferior to the kidney were used in abdominal cavity model (n = 6). Renal function was evaluated by daily measurement of plasma creatinine and BUN concentrations. The experiments' health including postoperative complications was also assessed daily for 8 days after transplantation. After euthanazation gross and histopathologic analysis was performed. All six pigs in iliac fossa model developed neuropraxia and lameness of the ipsilateral pelvic limb. However, no necrosis was observed in any pigs. In the abdominal cavity model, durations of both the surgical operation and the vascular anastomosis were significantly longer than those in the iliac fossa model. Furthermore, ischemia injury of the transplanted kidney was increased in abdominal cavity model, which induced accelerated-acute immune response from day 4 after transplantation. Despite of pelvic limb complication, the iliac fossa model showed more advantages including not only less ischemia time related to easy vascular anastomosis, but also less immune response during the acute rejection period. The results indicate that the iliac fossa model may be appropriate to the study of acute rejection in porcine kidney transplantation.

• Preventive Nutrition and Food Science
• /
• v.16 no.4
• /
• pp.394-407
• /
• 2011

Better understanding of the complex factors leading to human diseases will be necessary for both long term prevention and for managing short and long-term health problems. The underlying causes, leading to a global health crisis in both acute and chronic diseases, include finite global health care resources for sustained healthy human survival, the population explosion, increased environmental pollution, decreased clean air, water, food distribution, diminishing opportunities for human self-esteem, increased median life span, and the interconnection of infectious and chronic diseases. The transition of our pre-human nutritional requirements for survival to our current culturally-shaped diet has created a biologically-mismatched human dietary experience. While individual genetic, gender, and developmental stage factors contribute to human diseases, various environmental and culturally-determined factors are now contributing to both acute and chronic diseases. The transition from the hunter-gatherer to an agricultural-dependent human being has brought about a global crisis in human health. Initially, early humans ate seasonally-dependent and calorically-restricted foods, during the day, in a "feast or famine" manner. Today, modern humans eat diets of caloric abundance, at all times of the day, with foods of all seasons and from all parts of the world, that have been processed and which have been contaminated by all kinds of factors. No longer can one view, as distinct, infectious agent-related human acute diseases from chronic diseases. Moreover, while dietary and environmental chemicals could, in principle, cause disease pathogenesis by mutagenic and cytotoxic mechanisms, the primary cause is via "epigenetic", or altered gene expression, modifications in the three types of cells (e.g., adult stem; progenitor and terminally-differentiated cells of each organ) during all stages of human development. Even more significantly, alteration in the quantity of adult stem cells during early development by epigenetic chemicals could either increase or decrease the risk to various stem cell-based diseases, such as cancer, later in life. A new concept, the Barker hypothesis, has emerged that indicates pre-natal maternal dietary exposures can now affect diseases later in life. Examples from the studies of the atomic bomb survivors should illustrate this insight.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2001.11b
• /
• pp.43-48
• /
• 2001

This study was investigated to find out the optimum planting densities of Scutellaria baicalensis GEORGE about growth characters, yield components and yield at 3 planting densities by direct sowing cultivated after barley Stem length was long In dense planting of 20$\times$10cm and short in spacious planting of 30$\times$10cm and 40$\times$10cm by direct sowing cultivated after barley. Stem diameter was thick in spacious planting of 30$\times$10cm and 40$\times$10cm and was thin in dense planting of 20$\times$cm by direct sewing cultivated after barley. Length and dry weight of root per plant were decreased in dense planting of 20$\times$10cm and were increased in spacious planting of 30$\times$10cm and 40$\times$10cm by direct sowing cultivated after barley. Yield of dry root was highest in optimum planting density(30$\times$10cm:33 plants/$m^2$) by direct sowing cultivated after barley. The correlation coefficient between number of planting plant and stem length showed highly positive correlation. These characters of stem diameter, number of branches, main root length and yield of dry root mentioned above showed negative correlations with planting plants.

• Korean Journal of Plant Resources
• /
• v.15 no.1
• /
• pp.77-80
• /
• 2002

This study was investigated to find out the optimum planting densities of Scutellaria baicalensis George about growth characters, yield components and yield at 3 planting densities by direct sowing cultivated after barley. Stem length was long in dense planting of 20$\times$l0cm and short in spacious planting of 30$\times$l0cm and 40$\times$10cm by direct sowing cultivated after barley. Stem diameter was thick in spacious planting of 30$\times$10cm and 40$\times$10cm and was thin in dense planting of 20$\times$10cm by direct sowing cultivated after barley. Length and dry weight of root per plant were decreased in dense planting of 20$\times$ 10cm and were increased in spacious planting of 30$\times$10cm and 40$\times$10cm by direct sowing cultivated after barley. Yield of dry root was highest in optimum planting density(30$\times$10cm：33 plants/㎡) by direct sowing cultivated after barley. The correlation coefficient between number of planting plant and stem length showed highly positive correlation. These characters of stem diameter, number of branches, main root length and yield of dry root mentioned above showed negative correlations with planting plants.

• Journal of the Korean Society of Safety
• /
• v.26 no.5
• /
• pp.118-129
• /
• 2011

In this study, species resistant to forest fire were extracted from high trees through an investigation of combustion mechanism. Here, the average flameless ignition temperatures of living leaves, branches and barks of oak were respectively, $365^{\circ}C$, $440^{\circ}C$ and $435^{\circ}C$, and the average flameless ignition temperatures of living leaves, branches, barks and cones of pine tree were respectively, $320^{\circ}C$, $405^{\circ}C$, $435^{\circ}C$ and $363^{\circ}C$. It shows that generally, pine tree has a lower flameless ignition temperature than oak and thus, has a relatively high risk of flameless ignition. When comparing risk of fire depending on ignition characteristics, Quercus serrata had a low risk of stem fire, and Quercus acutissima and Quercus serrata had a low risk of crown fire, as well. When analysing risk of fire depending on a duration of flame, also, Quercus dentata had a low risk of stem fire, and Quercus variabilis Blume had a low risk of crown fire too. Lastly, when comparing risk of fire, according to heat release rates, Quercus acutissima had a low risk of stem fire, and Quercus acutissima and Quercus dentata had a low risk of crown fire, as well. In conclusion, it was observed that as fire-resistant species, Quercus serrata and Quercus dentata have a low risk of ignition, Quercus variabilis Blume has a short duration of flame after discharging ignition when a forest fire occurs, and Quercus acutissima, Pinus Rigida and Antipathes japonica Brook have low heat release rates.