• 한국작물학회지
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• 제47권6호
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• pp.486-491
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• 2002

일반계인 추청벼, 화성벼와 통일계인 남풍벼를 공시하여 질소(5수준), 분시방법(2수준) 및 유수형성기 차광처리(3수준)에 따른 영화수, 지경 분화수, 퇴화수 등 영화수 구성요소, 질소함량, 건물중, 비구조탄수화물 등을 조사하여 경엽의 질소보유량 및 건물중과 영화수 구성요소와의 관계, 그리고 영화형성 효율의 품종간 차이를 검토하였으며 그 결과를 요약하면 다음과 같다. 1. 단위면적당 1차지경수는 유수분화기와 그 후 15일 및 출수기, 3차지경수는 유수분화 후 15일 출수기의 경엽중의 질소보유량과 유의한 정의 상관을 나타내었다. 단위면적당 1차지 경수는 유수분화기의 경엽중, 2차지경수는 유수분화 후 15일의 경엽중과 유의한 정의 상관이 있었다. 2. 단위면적당 2차지경 퇴화수와 영화의 퇴화수는 유수분화 이후 15일간의 경중 증가량, 경중의 비구조탄수화물 증가량, 그리고 이때의 비구조탄수화물 함량과 유의한 부의 상관을 나타내었다. 3. 단위면적당 영화수는 출수기의 경엽중 질소보유량, 경업의 건물중과 유의한 정의 상관관계가 있었다. 4. 출수기 경엽중의 질소보유량당 영화수로 표현한 영화형성호율은 통일계인 남풍벼가 일반계인 화성벼와 추청벼보다 높았으며, 영화형성효율은 건물중이 증가함에 따라 감소하였다. 이 기간중 엽의 건물중과는 상관관계가 없었으나 경의 건물 중 증가에 따라서는 모든 품종에서 유의하게 감소하였다. 생식생장기간 동안의 건물 중 증가에 따른 영화형성효율은 구조 탄수화물의 증가에 따라서 감소되었으며, 출수기 경중의 저장 탄수화물 함량이 높은 경우 영화형성효율이 높아지는 경향이었다.

• 식물조직배양학회지
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• 제25권2호
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• pp.99-102
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• 1998

백미조생, 천홍, 유명 등 3가지 복숭아 품종에 있어서 기내 신초 유기에 적합한 배지는 LP 배지이며, 기내 신초 증식에 효과적인 탄소원은 20 g/L sorbitol 처리와 30 g/L sorbitol 처리였다. 배지내 500mg/L LH를 첨가함으로써 백미조생 품종의 기내 신초 증식율의 향상을 가져올 수 있었으며 신초정부를 제거하고 수평배양하는 것이 기내 신초중식에 효과적이었다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2003년도 제10차 국제학술회의 및 추계정기 학술발표회
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• pp.2-3
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• 2003

A method for rapid propagation of mature Jack fruit was developed. Four types of explants (mature embryos, apical meristems of young seedlings, apices from mature plants and nodal segments) were used. It has been found 88% of young apical meristems produced shoots in Campbell and Durzan (CD) medium compared to 60% in Murashige and Skoog (MS) medium. Only 1/3 of them produced multiple shoots. Shoot initiation from nodal segments was very rare. Mature apices produced callus. Although removal of the sheathing cover around mature buds enhanced the shoot initiation but success rate was low in growth regulator free medium. Embryos respond to the CD medium but not to the MS medium. Embryos from seeds soaked in water for 24 hours produced shoots after 8 weeks of incubation and the success rate was 70% while embryos from dry seeds only produced roots. There was no significant effect of cold storage (refrigeration) for 7 days on shoot initiation from mature embryos (65%) but the ability for shoot induction declines with storage time (55% after 21 days of cold storage). Mature axillary buds were established in Modified Campbell and Durzan (CD) medium supplemented with 0.5mg/1 and IBA. There was a significant difference in the growth performance of shoots according to the period of the year in which explants were collected. Highest (60%) was observed in November-January period. It was only 30% when the explants were collected in February-April or May-July and decreased to 20% in August-October. The shoots produced in November-January showed a higher vigor than those produced in other months. Since Jak fruit show seasonal changes in fruit bearing and shedding of leaves, it can be suggested that the difference in growth performances of tissues cultured in artificial culture media would have been affected by endogenous rhythms.

• 한국작물학회지
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• 제47권5호
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• pp.390-394
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• 2002

Spikelet number per unit area(SPN) is a major determinant of rice yield. Nitrogen nutrition status and biomass during reproductive stage determine the SPN. To formulate a model for estimating SPN, the 93 field experiment data collected from widely different regions with different japonica varieties in Korea and Japan were analyzed for the upper boundary lines of SPN responses to nitrogen nutrition index(NNI), shoot dry weight and shoot nitrogen content at panicle initiation and heading stage. The boundary lines of SPN showed asymptotic responses to all the above parameters(X) and were well fitted to the exponential function of $f(X)=alphacdot{1-etacdotexp(gamma;cdot;X)}$. Excluding the constant, from the boundary line equation, the values of the equation range from 0 to 1 and represent the indices of parameters expressing the degree of influence on SPN. In addition to those indices, the index of shoot dry weight increase during reproductive stage was calculated by directly dividing the shoot dry weight increase by the maximum value ($800 extrm{g/m}^{-2}$) of dry weight increase as it showed linear relationship with SPN. Four indices selected by forward stepwise regression at the stay level of 0.05 were those for NNI ($I_{NNI}_P$) at panicle initiation, NNI($I_{NNI}_h$) and shoot dry weight($I_{DW}_h$) at heading stage, and dry weight increase($I_{DW}$) between those two stages. The following model was obtained: SPN=48683ㆍ $I_{DWH}$$^{0.482}$ㆍ $I_{NNIp}$$^{0.387}$ㆍ $I_{NNIH}$$^{0.318}$ㆍ $I_{DW}$ $^{0.35}$). This model accounted for about 89% of the variation of spikelet number. In conclusion this model could be used for estimating the spikelet number of japonica rice with some confidence in widely different regions and thus, integrated into a rice growth model as a component model for spikelet number estimation.n.n.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2003년도 심포지엄
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• pp.9-18
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• 2003

A method for rapid propagation of mature Jack fruit was developed. Four types of explants (mature embryos, apical meristems of young seedlings, apices from mature plants and nodal segments) were used. It has been found 88% of young apical meristems produced shoots in Campbell and Durzan (CD) medium compared to 60% in Murashige and Skoog (MS) medium. Only 1/3 of them produced multiple shoots. Shoot idtiation from nodal segments was very rare. Mature apices produced callus. Although removed of the sheathing cover around mature buds enhanced the shoot initiation but success rate was low in growth regulator free medium. Embryos respond to the CD medium but not to the MS medium. Embryos from seeds soaked in water for 24 hours produced shoots after 8 weeks of incubation and the success rate was 70% while embryos from dry seeds only produced roots. There was no significant effect of cold storage (refrigeration) for 7 days on shoot initiation from mature embryos (65%) but the ability for shoot induction declines with storage time (55% after 21 days of cold storage). Mature axillary buds were established in Modified Campbell and Durzan (CD) medium supplemented with 0.5mg/1 and IBA. There was a significant difference in the growth performance of shoots according to the period of the year in which explants were collected. Highest (60%) was observed in November-January period. It was only 30% when the explants were collected in February-April or May-July and decreased to 20% in August-October. The shoots produced in November-January showed a higher vigor than those produced in other months. Since Jak fruit show seasonal changes in fruit bearing and shedding of leaves, it can be suggested that the difference in growth performances of tissues cultured in artificial culture media would have been affected by endogenous rhythms.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 춘계학술대회 및 국제심포지움 초록집
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• pp.6-9
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• 2005

Plant biotechnology involving genetic modification has been rather controversial. However, the major issues related to safety are being addressed by continued improvements in technology. Some of the related facts will be highlighted to set the tone for a scientific discussion on the possibilities of using the technology for crop improvement. Our main research interest is to understand the molecular regulation of shoot bud regeneration in plant tissue culture, which is essential for crop improvement by biotechnology. We have isolated and characterized some genes that are associated with adventitious shoot regeneration. These include a MADS-box cDNA (PkMADS1) from paulownia kawakamii, which regulates vegetative shoot development and in vitro shoot regeneration from leaf explants. Another gene we have characterized from petunia codesfor a cytokinin binding protein (PETCBP). Preliminary functional analysis of this gene indicated that this also affects adventitious shoot bud initiation. Also, the antisense suppression of this gene in petunia causedexcessive branching. Results from our work and selected other publications will be used to highlight the possibilities of manipulation of such genes to improve crop species.

• 한국산림과학회지
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• 제46권1호
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• pp.21-28
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• 1980

채종원(採種園)에서 18년생(年生) 접목묘(接木苗)인 슬래쉬 소나무를 대상(對象)으로 하여, 구과형성(毬果形成)과 가지의 형태학적(形態學的)인 상관관계(相關關係)를 조사했다. 춘기(春期)에 조사한 지당(枝當) 자화수(雌花數)와 상관관계가 가장 큰 것은 전년도(前年度) 신초지(新梢枝)의 지당(枝當) 엽중량(葉重量)이었다. ($R^2=+0.41$). 웅화수(雄花數)는 신초지(新梢枝)의 엽중량(葉重量)과 부(負)의 상관(相關)을 보여주었다. ($R^2=-0.21$). 웅화(雄花)에서 자화(雌花)로의 성전환(性轉換)은 그 가지의 생장력(生長力)(vigor)이 증가함에 따라서 촉진(促進)되었다. 자화(雌花)가 거의 착화(着花)되지 않는 개체목(個体木)의 토부수관(土部樹冠)에서 채취(採取)한 정아(頂芽)와, 자화(雌花)나 웅화(雄花)가 대량(大量)으로 착화(着花)한 개체(個体)의 정아(頂芽)로, 유관속 조직의 생장(生長)을 비교(比較)한 결과, 전자(前者)가 후자(後者)보다 훨씬 빨랐으며, 이는 자화(雌花)를 생산(生産)하지 않는 정아(頂芽)는 강(强)한 영양생장(營養生長)의 경향(傾向)을 나타냄을 뒷받침하는 것이다.

• 한국작물학회지
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• 제43권2호
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• pp.71-76
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• 1998

To define the relations between endogenous GA levels and growth and flowering in short-day plant sorghum, growth retardant BX-112 was applied to two sorghum genotypes, wild-type and phytochrome B mutant (phyB-1), which grows faster and flowers earlier than the wild-type. BX-112 and $GA_3$ were applied as a soil drench, and plant height, culm length, and date to floral initiation were investigated. Endogenous GAs contents were measured with GC-MS-SIM. BX-112 treatments inhibited shoot growth in both genotypes and drastically reduced $GA_1$ and $GA_8$ levels. With increasing BX-112 concentrations, $GA_1$ concentrations declined linearly, but caused the accumulation of intermediates from $GA_12$ to $GA_20$. This result implies that $GA_1$ is the major active endogenous GA in shoot elongation in a short day plant sorghum. The inhibition of plant growth in both of wild type and phyB-1 by BX-112 was very similar, while BX-112 effects on floral initiation in two types of plants differed significantly. Floral initiation of phyB-1 was not affected by BX-1l2, but that of wild-type was delayed as BX-1l2 concentration increased. Because BX-112 treatment causes accumulation of biosynthetic intermediates between synthetic pathway from $GA_12$ to $GA_20$ and because phyB-1 is altered in GA metabolism in this same region of the early C13-hydroxylation pathway, BX-112 may fail to block flowering of phyB-1.

• 식물조직배양학회지
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• 제25권6호
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• pp.515-518
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• 1998

Ethylene이 옥화란 rhizome의 증식과 유식물체의 분화에 미치는 영향을 조사하였다. Ethylene 발생량은 rhizome 치상 8일째 가장 많았고 배양기간 동안의 총 ethylene 발생량은 rhizome이 증식될 때보다 유식물체가 분화될 때 많았다. 배양 rhizome에 ethephon을 처리한 결과 ethephon은 rhizome의 증식과 유식물체의 길이생장은 억제하였으나 유식물체의 분화에는 효과적이었다. Aminoethoxyvinylglycine(AVG) 1mg/L 처리에서는 rhizome의 증식과 유식물체의 분화에는 효과적이었으나 10mg/L에서는 rhizome의 증식이 억제되었고 유식물체의 길이생장은 촉진되었다. $\textrm{AgNO}_{3}$처리는 rhizome의 증식과 유식물체의 분화를 억제하였다.

• Molecules and Cells
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• 제27권6호
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• pp.615-620
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• 2009

The plant shoot meristem maintains a group of stem cells that remain active throughout the plant life. They continuously generate new cells that are then recruited for organ initiation in the peripheral zone. Stem cell proliferation and daughter cell differentiation has to be integrated with overall growth and development of the diverse functional domains within the shoot apex. Several studies have revealed extensive communication between these domains. The signaling mechanisms employed comprise diffusible peptides, directional transport of plant hormones, but also complex interactions between transcription factors, that together establish a panoply of regulatory inputs that fine-tune stem cell behavior in the shoot meristem.