• 한국연초학회지
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• 제14권1호
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• pp.33-41
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• 1992

These studies were conducted to investigate effects of phytohormone and activated carbon on the growth and rooting of teratoma shoots induced from Nicotiana tabacum cv. NC2326 transformed by Aerobacterium tumefaciens C58. GA was effective for shoot elongation and reduction of multiple shoots from teratoma shoot, however, leaves of teratoma shoot cultured on the medium with GA were pointed. ABA was also effective in promoting shoot elongation, but was not for reduction of multiple shoots. Teratoma shoot cultured on the medium with 1 n activated carbon promoted shoot elongation and inhibited the number of shoots differentiated, but was grown as abnormal shoot. Addition of 1% activated carbon and 0.5mg/l BA to culture media was effective for shoot elongation and reduction of multiple shoot and for formation of round leaves as normal leaves. Though these shoots were inoculated on the rooting medium, they could not from roots but formed multiple shoots. Boric acid, myo-inositol and sucrose were also ineffective on the rooting of teratoma shoots.

• 한국약용작물학회지
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• 제9권2호
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• pp.146-149
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• 2001

이 연구는 지황에서 신초의 유도와 신장에 미치는 thidiazuron과 paclobutrazol의 영향을 조사분석한 것으로 얻어진 결과는 아래와 같다. 1. 신초 유도시 thidiazuron을 단독처리한 경우에 신초 발생율이 높았으며, thidiazuron은 저농도에서부터 활성을 나타내어 영향을 주는 농도범위가 비교적 넓었다. 특히 thidiazuron 1.0mg/L 농도에서 신초 발생이 많았다. 2. 신초 유도시 thidiazurona만을 사용하였거나 thidiazuron에 paclobutrazol을 조합처리하였던지 간에 신초의 신장에는 모두 thidiazuron 1.0mg/L 처리구에서 마디수와 신초의 수가 가장 많아 신초 신장에 효과적이었다.

• 한국연초학회지
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• 제13권1호
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• pp.36-42
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• 1991

The present study was conducted to obtain some basic information on the shoot formation from crown gall callus and the characteristics of teratoma shoot derived from crown gall induced by inoculation of Agrobacterium tumefaciens C58. Crown gall callus could be continuously cultured on the phyohormone free basic medium. The growth of crown gall callus was inhibited when BA were added to the cultural media. Shoot formation from crown gall callus fail to be initiated except teratoma shoot which induced on the phytohormone free medium after several subculture on rare occasions. Teratoma shoot could not form root and grow as normal shoot. Addition of BA to cultural media was not effective for shoot elongation, reduction in multiple shoot formation, but IBA was somewhat effective for shoot elongation of teratoma shoot, never for root formation.

• 한국작물학회지
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• 제51권4호
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• pp.287-294
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• 2006

Germination and early elongation of rice after germination were investigated in anoxic air treatment, nitric oxide gas treatment, and six concentrations of mercuric chloride solutions to determine the effects of limited oxygen environment, nitric oxide, and inhibited water flux through cell membrane in $17^{\circ}C$. Anoxic air treatment affected germination of tested six varieties very little. However root elongation rates were severely inhibited while shoot growth was affected less. Reductions in shoot and root elongations demonstrated genotypic variations. Nitric oxide delayed the germination of rice even though it didn't affect the final percent germination. Elongations of root and shoot were inhibited in nitric oxide treatment. The inhibitor effect of nitric oxide on the shoot elongation of rice was less severe, while nitric oxide completely inhibited the root emergence of rice. Concentrations of $HgCl_2$ greater than $300{\mu}M$ dramatically reduced the rate and percentage of germination when compared to distilled water treatment. The reduced percent germination showed the greatest variation among rice varieties in $500{\mu}M$ solution of mercuric chloride. Ansanbyeo, Jinheung, and Odaebyeo were affected less by $HgCl_2$, Nonganbyeo and Sangmibyeo were intermediate, and the germination of Andabyeo was greatly reduced by $HgCl_2$. Root elongation of germinated rice seedlings was more sensitive to oxygen deficits, nitric oxide, and $HgCl_2$ treatments than germination and shoot elongation. In conclusion, poor seedling establishment of rice sown in flooded paddy soils, in which the oxygen supply to the seeds is restricted, appears to the result of limited root elongation rate.

• 한국자원식물학회지
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• 제28권3호
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• pp.357-362
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• 2015

Carbohydrate sources are one of important factors associated with macro- and micro nutrients and phytohormones in vitro culture medium for shoot growth. The optimal carbohydrates for eight species of the genus Prunus which are economically important fruit crop was evaluated at the initiation and elongation stages. All carbohydrate seemed utilized for the bud break and leaf growth at the early stage of culture. However, shoot elongation and fresh weight of species tested were superior in the medium containing 90 mM of fructose or glucose rather than sucrose. There was no difference between glucose and fructose. Adventitious shoots from the axillary buds were induced in most species but no significant differences were observed except for two species (P. salicina ‘Shiro’ and P. tomentosa). These result demonstrated that glucose and fructose were suitable carbohydrate sources for diverse Prunus species than sucrose, although the response to the carbohydrates in the medium were slightly different in the species.

• 식물조직배양학회지
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• 제28권4호
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• pp.185-188
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• 2001

Spathiphyllum을 기내에서 증식시킨 후, 액체배지를 첨가하여 신초의 생장 및 발근을 촉진시키고, 기외에서 순화율을 향상시켜 생산비를 감소시키기 위하여 일련의 실험을 실시하였다. 3∼4개의 신초를 가지고 있는 Spathiphyllum floribundum 'Cupid' 의 신초 cluster 절편체를 BA 2.0 mg/L가 첨가된 LS배지에서 8주간 증식시킨 후에 15 mL의 액체배지를 동일용기에 첨가하였다. 액체배지 첨가는 1/2 MS 다량요소+sucrose 50 g/L +활성탄 5.0∼10.0 g/L가 첨가된 배지 15 mL를 첨가하는 것이 식물체의 기내생장 및 발근에 양호하였으며, 온실에서 perlite와 vermiculite가 1 : 1로 혼합된 용토에 발근된 식물체를 재식하면 95% 이상 생존하였다.

• 한국작물학회지
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• 제62권3호
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• pp.259-274
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• 2017

Platycodon grandiflorum (Bell flower) is an important plant that has traditionally been used as herbal medicine for the treatment of cough, phlegm, sore throats, lung abscesses, chest pains, dysuria, and dysentery. The present study was initiated to investigate the feasibility of inducing shoot and root organogenesis in cultured explants of P. grandiflorum in a range of culture media and through use of various plant growth regulators (PGRs). The plantlets (Stem containing one node) were isolated and cultured on different concentrations of Murashige and Skoog (MS) medium supplemented with PGRs. We found that proliferation and elongation of shoots and roots could be achieved on 1/4 MS for P. grandiflorum with wild and green petals and on 1/8 MS for P. grandiflorum with double petals. The highest levels of development and elongation of adventitious shoots and roots were observed when petal explants were cultured on 1/4 MS (pH 3.8) supplemented with 5% sucrose. Increasing the agar concentration reduced shoot growth and rooting potential; nevertheless, the highest number of shoots and roots was observed on 0.6% agar. In the case of growth regulators, 1/4 MS supplemented with $1mg\;L^{-1}$ 6-benzylaminopurine (BA) was found to be best for shooting, although higher concentrations of BA tended to reduce shoot and root elongation. The highest number of shoots was achieved on $0.5mg{\cdot}L^{-1}$ thidiazuron (TDZ) from double petal explants grown on 1/8 MS. However, root and shoot elongation were found to decrease when TDZ concentrations were increased. Low concentrations of kinetin, naphthalene acetic acid, indole acetic acid, and 3-indole butyric acid induced shoot and root proliferation and elongation. Taken together, our study showed that low concentrations of PGRs induced the greatest root formation and elongation, showing that the optimal concentration of PGRs for shoot proliferation was species-dependent.

• Journal of Plant Biotechnology
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• 제32권3호
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• pp.195-199
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• 2005

홍경천(Rhodilola sachalinensis A. Bor)의 잎절편로부터 부정아를 유도하고자 BA와 kinetin을 처리한 결과, BA의 처리가 kinetin의 처리보다 부정아 유도와 신초의 생장에 양호한 것으로 나타났으며, 특히 BA 1 mg/L처리에서 부정아 유도빈도와 신초길이는 각각 71%와 3.0 mm로 가장 높게 나타났다. 또한 고빈도의 부정아를 유도하고자 다양한 농도의 putrescine를 처리한 결과, putrescine 100 mM 처리에서 부정아 유도빈도가 BA 1 mg/L 단용처리보다 약 20%가 증가되었고 신초길이 역시 약 10 mm가 증가된 것으로 나타났다. 이상의 결과에서 유도된 부정아는 생장조절물질이 포함되지 않은 1/2MS배지에서 발근과 신장이 정상적으로 이루어졌으며 약 7 cm 정도 생장한 유식물체는 peatmoss:sand가 1:1로 혼합된 상토에서 약 95%이상 정상적으로 순화되었다.

• 생명과학회지
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• 제7권2호
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• pp.134-141
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• 1997

미류나무 잎절편 조직배양에 의한 식물체 재분화에 관한 여러 가지 여인을 조사한 결과를 요약하면 다음과 같다. 1. 5가지 각기 다른 농도를 가진 培地에 줅,절편을 치상하여 側牙의 발달을 유기시킨 결과, WPM이 MS배지 보다 더 좋은 반응을 보였고 또한 배지내의 NH$_{4}$NO$_{3}$의 양은 측아 증식에 큰 영향을 미치지 않는 것으로 관찰되었다. 2. 기내에서 증식된 잎을 3부분으로9윗, 중간 그리고 및) 절단하여 2종류의 식물성장 절물질(TDZ와NAA)의 16가지의 농도배합을 사용하여 배양한 결과 TDZ0.01mg/1와 NAA 0.02 mg/1에서 가장 높은 부정줄기 형성체들이 생성되었다. 또한 잎의 윗 부분 보다는 중간과 밑부분에서 높은 부정줄기 형성체들이 생성되었는 것을 관찰하였다. 3. 3가지 탄수화물을 사용하여 부정줄기의 유기를 관찰한 바, sucrose 나 glucose보다 fructose를 첨가한 배지에서 좋은 반응을 보여 주었다.

• 한국환경농학회지
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• 제40권4호
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• pp.359-365
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• 2021

BACKGROUND: Prohexadione-calcium (PC) is a plant growth regulator of cyclohexanedione family acting on inhibiting gibberellin biosynthesis and has been used to regulate shoot elongation of turfgrass in the golf course during the summer season. This study was conducted to investigate the effects of PC on the inhibition of shoot elongation, growth, and nitrogen uptake in Zoysia matrella. METHODS AND RESULTS: Treatments were as follows; non-treatment, control (TE 0.01 a.i. g/m²/100 mL), 0.0025PC (PC 0.0025 a.i. g/m²/100 mL), 0.005PC (PC 0.005 a.i. g/m²/100 mL), 0.01PC (PC 0.01 a.i. g/m²/100 mL) and 0.02PC (PC 0.02 a.i. g/m²/100 mL). Visual quality and chlorophyll content were not significantly different. As compared to non-treatment, shoot length and clipping yield in 0.005PC, 0.01PC and 0.02PC treatments were decreased by 29-36% and 65-82%, respectively, and those of 0.0025PC were not significantly different. N uptake of Z. matrella after applying PC was decreased by 28.9-77.8% than that of non-treatment. Inhibition effects of PC treatment in Z. matrella were not significantly different from those of control. CONCLUSION(S): These results indicated that the application of prohexadione calcium inhibited shoot elongation, clipping yield and N uptake amount in Z. matrella, and trinexapac-ethyl could be replaced with prohexadione-calcium in turfgrass management.