• Plant Resources
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• 제6권3호
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• pp.233-241
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• 2003

The shoot tip and young leaf of Rhodiola sachalinensis were cultured to invest the plant growth regulator condition for callus induction, shoot and root regeneration. When the shoot tip was sterilized in 2.0% of NaOCl for 20min., the contamination rate was the lowest. And the survival rate of the culture material was good in carbenicillin 500mg/L treatment group. Callus was obtained from shoot tip and young leaf segments. NAA 0.1-1.0mg/L and 2,4-D 0.1-0.5mg/L alone treatment were shown to have a good response on callus induction from shoot tip culture. In the case of young leaf culture, NAA and 2,4-D 0.1-0.5mg/L alone treatment were good in callus induction. In culturing shoot tip NAA 0.5mg/L and BA 0.5mg/L, NAA1.0mg/L and BA 0.lmg/L combination treatment was good in shoot regeneration. The regenerated shoots were rooted on MS medium supplemented with NAA and BA combination treatment. Especially, NAA 1.0mg/L and BA 0.1mg/L combination treatment was effective for root regeneration.

• 한국초지조사료학회지
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• 제11권2호
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• pp.90-96
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• 1991

This experiment was carried out in order to estimate the recovery days of root and stubble to the days after cutting, and contribution of specific stubble weight on the regrowth was examined using the relationships between the dry weight of shoot and yield components, and regrowth parameters by the days after cutting. The varieties examined were Maprima, Manhattan, Tove, Peramo, Caliente, Tempo and P-2 grown under individual plant basis. The results are may be summarized as follows: 1. Dry weight of root and stubble were recovered up to 13.5 and 11 days after cutting, respectively. 2. Dry weight of shoot(regrowth parts+stubble) was affected significantly by the varieties, stages of regrowth and variety x stage of regrowth. 3. The variety with tiller weight type showed higher average productivity of shoot than those of the variety with tiller number type. 4. Absolute growth rate(AGR) of shoot was correlated significantly with regrowth parts, stubble, root and weight of a tiller at the early stage of regrowth(up to 12 days after cutting), and correlated with regrowth parts, stubble, weight of tiller and stubble area at the late stage of regrowth(up to 20 days after cutting). 5. Contribution of specific stubble weight to absolute growth rate of shoot was different between the stages of regrowth. Thus, regrowth parts per specific stubble weight(RP1SSbW) and weight of tiller per specific stubble weight(WT1SSbW) contributed to absolute growth rate of shoot at the early stage of regrowth, and efficiency of specific stubble weight(ESSbW), regrowth parts per specific stubble weight (RPISSbW) and weight of a tiller per specific stubble weight(WT1SSbW) contributed to absolute growth rate of shoot at the late stage of regrowth. 6. Regrowth utilization rate(RUR) was one of the useful regrowth parameter to indicate the regrowth potential of grasses.

• 농업생명과학연구
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• 제44권6호
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• pp.39-44
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• 2010

An efficient method for the rapid propagation of Smilax china from axillary buds was established. Plants with thick leafage were selected from Korea native S. china population. Axillary buds of S. china collected from selected plant and were cultured in various culture media (2MS, MS, 1/2MS, WPM, B5 and SH medium). Shoot was induced from axillary bud on MS basal medium after 4 weeks of culture. 1/2MS medium showed a higher growth rate than those of the others, while the lowest shoot growth was obtained in 2MS medium. Among the sucrose concentrations, 5% sucrose was the optimum level for shoots growth from axillay buds. Among cytokinins, $0.5mgL^{-1}$ 6-benzylaminopurine (BAP) treatment showed the best performance on shoot multiplication, yielding average shoot multiplication forming about 2.4. Rooting was induced directly near the base of the shoot on 1/2MS medium containing with three-auxins ${\alpha}-napthalene$ acetic acid (NAA), indole acetic acid (IAA) and ${\beta}-indolebutyric$ acid (IBA) (0.5 and $1.0mgL^{-1}$). The $1.0mgL^{-1}$ IBA treatments induced earliest rooting with maximum of root number and root growth. These rooted plantlets were successfully transferred to pots for 4 weeks hardening process, and were transferred to soil with above 90% survival rate.

• 한국자원식물학회지
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• 제28권3호
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• pp.357-362
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• 2015

Carbohydrate sources are one of important factors associated with macro- and micro nutrients and phytohormones in vitro culture medium for shoot growth. The optimal carbohydrates for eight species of the genus Prunus which are economically important fruit crop was evaluated at the initiation and elongation stages. All carbohydrate seemed utilized for the bud break and leaf growth at the early stage of culture. However, shoot elongation and fresh weight of species tested were superior in the medium containing 90 mM of fructose or glucose rather than sucrose. There was no difference between glucose and fructose. Adventitious shoots from the axillary buds were induced in most species but no significant differences were observed except for two species (P. salicina ‘Shiro’ and P. tomentosa). These result demonstrated that glucose and fructose were suitable carbohydrate sources for diverse Prunus species than sucrose, although the response to the carbohydrates in the medium were slightly different in the species.

• Journal of Plant Biology
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• 제17권2호
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• pp.95-98
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• 1974

This experiment was undertaken with the purpose of investigating effect of gibberellin on the growth and electron microscopic ultrastructure of hop(Humulus lupulus var. Hallertau) shoot apex. The results are as follows. 1) The growth of stem in the plots treated with GA(10, 25, and 50ppm) is more predominant than control. The leaves and cones of treated plots turn to pale green. 10ppm plot of GA treatment is the most effective in growth and yield than the others. 2) Under the GA-treated condition, it is believed that the nucleoplasmic index (NP) are higher than the control, and so nucleus, nucleolus, mitochondria, primary cell wall, middle lamella, and etc. tends to be larger than the control. 3) Mitochondria contained in the cell of shoot apex treated with concentration of 10ppm GA was seemed to increases in number and be larger in size than the control.

• Advances in environmental research
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• 제4권1호
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• pp.1-15
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• 2015

A pot experiment was conducted to assess the effects of red pepper (Capsicum annuum) and coriander (Coriandrum sativum) on seedling growth of wheat (Triticum aestivum). The aqueous extracts treatment of red pepper and coriander showed a significant (p < 0.05) reduction in root, shoot and seedling length, number of leaves and seedling dry weight of wheat (T. aestivum) as compared to control. The inhibitory different effect on growth of wheat (T. aestivum) was directly proportional to the increasing concentration (1, 2, 3, 4 and 5%) of aqueous extracts of red pepper and coriander as compared to control treatment (0%). The root, shoot, seedling length and number of leaves of T. aestivum significantly p < 0.05 decreased at 5% concentration of red pepper as compared to control. The root, shoot and seedling growth of T. aestivum was also significantly reduced at 1, 2, 3, 4 and 5% concentration of coriander as compared to control. The root, shoot and leaves dry weight of T. aestivum at 5% coriander extract treatment concentration decreased as compared to control. The tolerance in seedlings of T. aestivum to red pepper and coriander extract treatment was dose dependent as compared to control. The seedlings of T. aestivum showed low percentage of tolerance to pepper extract treatment than coriander extract treatment.

• 원예과학기술지
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• 제30권1호
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• pp.13-20
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• 2012

본 연구는 스탠다드 국화 '백마' 품종의 플러그 묘 생산에 있어서 삽수의 조건이 지상부 생육과 발근 및 정식 후의 절화 생육에 미치는 영향을 알아보고자 실시하였다. 삽수에 잎을 많이 부착할수록 삽목묘의 지상부 생육이 양호하였고, 잎을 4-6장 부착하여 삽목하는 것이 다른 처리에 비해 발근에 효과적이었다. 삽수길이에 따른 삽목묘의 지상부 생육은 큰 차이가 없었으며, 삽수길이를 5-7cm로 처리하는 것이 발근상태가 양호하였다. 삽수 굵기는 3.1mm 처리에 비해 3.6mm와 4.2mm 처리에서 삽목묘의 지상부 생육과 발근이 양호하였다. NAA 처리농도가 높을수록 삽목묘의 지상부 생육이 억제되었으며, 뿌리의 길이도 짧아졌다. NAA $100mg{\cdot}L^{-1}$를 1시간 처리하여 삽목하는 것이 삽목묘의 지상부 생육과 발근에 효과적이었다. 피트모스와 펄라이트를 2:2로 혼합한 배지에서 다른 처리에 비해 뿌리의 길이, 수 및 무게 등이 뿌리의 생육이 양호하였다. 잎이 적은 것보다 4-6개 부착된 삽수의 삽목묘를 정식했을 때, 절화장이 길고, 무게가 무거웠으며, 엽수도 많았다. 삽수길이에 있어서 7-9cm 처리가 절화장, 절화무게, 설상화 수 등 전반적인 절화의 생육에 효과적이었다. 따라서 '백마' 플러그 묘 생산시 7cm 길이에 3.6mm 굵기의 4-6장의 잎을 부착한 삽수를 삽목하는 것이 삽목묘의 지상부 생육과 발근 및 정식 후의 절화 생육을 위해 바람직하였다.

• Journal of Ecology and Environment
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• 제35권4호
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• pp.343-349
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• 2012

The use of growth medium is often recommended milkweed seedlings to grow and develop after emergence, and it is affected by growth medium and local habitat conditions. The effects of growth medium and partial shade on early growth of milkweed under drought stress (Calotropis procera L.) were studied in a field experiment. A split-split plot experimental design with three replications was carried out in the nursery. The main treatment plot was divided into two levels of shade; (no shading and partial shading). Sub treatment plot1 included growth medium at four levels (G1 = clay [suitable for milkweed growth], G2 = clay + sand, G3 = clay + perlite, G4 = clay + perlite + sand) and sub treatment plot2 included drought (irrigation intervals) at six levels (D1 = 2 [control], D2 = 4, D3 = 6, D4 = 8, D5 = 10, and D6 = 12 days per for three month). The results showed that drought stress significantly decreased emergence percentage, shoot length, shoot dry weight (SDW1), root dry weight (RDW), seedling dry weight (SDW2) and vigor index (VI). The use of growth medium increased all seedling characteristics. The G3 (clay + perlite) growth medium showed the highest performance, especially in terms of emergence percentage and seedling dry weight. Partial shade improved shoot length, shoot dry weight, and vigor index. Our results showed that the best treatment for high-vigor milkweed seedlings under drought stress was G3 (clay + perlite) growth medium and partial shade.

• 한국약용작물학회지
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• 제14권4호
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• pp.225-228
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• 2006

식물생장조절제의 종류와 농도를 모색하여 할미꽃의 발근 최적 조건을 조사하여 정상적인 재분화체를 획득하고자 하였다. 지금까지의 선행연구를 토대로 최적조건에서 shoot를 유도시킨 후 발근 유도 생장조절제에 바로 옮기면 shoot가 너무 작아 재분화체가 성장하지 못하고 고사되었다. 1/2 MS에서 키운 shoot를 3.0 mg/L NAA를 첨가한 배지에서 3일간 생장시킨 후, 1/3 MS로 옮겨 치상 한 경우, 약 10일 전후에 뿌리의 유도가 관찰되었다. 이렇게 뿌리가 유도된 재분화체는 염의 농도와 설탕의 농도를 동시에 순차적으로 낮추어 순화처리되었다.

• Journal of Plant Biotechnology
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• 제46권2호
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• pp.114-118
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• 2019

Efficient protocol for plant shoot regeneration of Brassica juncea L. CZERN was established by using organic media components and growth stimulating factors of the vermicompost and coelomic fluids. Formulated organic plant tissue culture media (Vermicompost (30%) extracts supplemented with 20 mL/L coelomic fluid) have shown maximum shoot regeneration when compared with the Murashige and Skoog (MS) medium, which were supplemented with 1 mg/L 6-benzyladenine (BA) and 0.1 mg/L of Naphthaleneacetic acid (NAA). Cotyledon explants produced the highest shoot regeneration frequency from fourday-old germinated seedlings in comparison with non-germinated seedlings. The vermicompost extracts have proved to be the best organic plant growth media to induce shoots from cotyledons compared to the MS media. Statistically significant difference (P = 0.008) for the root length, shoot length (P=0.000350) and the leaves (P=0.375) of the mustard plantlets were analyzed successfully. The survival rate was 98% in the mustard cotyledons on the Vermicompost extract media and 63% on MS media respectively. The coelomic fluid also is much suitable to induce shoots from cotyledons at lower concentrations. It was also shown that the vermicompost extract, which comprised of humic acids along with coelomic fluid, affected shoot regeneration from the cotyledons. An efficient and organic shoot regeneration study was standardized and it can be applicable in the improvement of the economically important crops.