• Title/Summary/Keyword: Shaking method

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Studies on the Glucose Isomerizing Enzyme -Part III The Effects of Metal Ions on the Reaction of K-17 Strain's Glucose Isomerizing Enzyme- (포도당(葡萄糖) 이성화(異性化) 효소(效素)에 관(關)한 연구(硏究) -제3보(第三報) 분리(分離) 선정(選定)된 균주(菌株) 효소(酵素)의 작용(作用)에 미치는 금속염(金屬鹽)의 영향(影響)에 대(對)해서-)

  • Seu, J.H.;Kim, C.K.;Ki, W.K.;Rhee, I.K.;Kwon, T.J.;Woo, D.L.
    • Applied Biological Chemistry
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    • v.11
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    • pp.55-61
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    • 1969
  • This K-17 strain was not absolutely requiring xylose as an inducer for enzyme formations. The most activity of this enzyme was lost when treated at $75^{\circ}C$. for 30 hours but was not influenced at $70^{\circ}C$. for 70 hours of treatment. The activity of this enzyme was increased by the addition of magnesium ions or cobalt ions in the reaction system. In the studies, we found that the magnesium ions simply activate the enzyme reaction and the cobalt ions do not but protect the enzyme from heat inactvation. And it was also found the phosphate buffer solution was very suitable as glucose dissolving solvent on the enzyme reaction. The mixed carbon source medium containing glucose, fructose, sorbitol, xylose and sucrose was more favorable for enzyme production then a sole carbon source containing medium on the shaking culture method.

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Condenser Characteristics of Dielectric Soil Moisture Sensor (유전율 토양 수분 쎈서의 콘덴서 특성)

  • Oh, Yong-Taeg;Eorn, Ki-Cheol;Jo, In-Sang;Shin, Jae-Sung
    • Korean Journal of Soil Science and Fertilizer
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    • v.33 no.1
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    • pp.15-23
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    • 2000
  • RC oscillation method was applied to study the condenser characteristics of two metal sticks insulated by vinyl tube and used in the dielectric constant determinations of most soils. Its capacitance as influenced by the contacted ambient materials was measured as relative capacitance of the sensor sticks compared with the standard one on the RC oscillation circuit. According to the equivalent circuit of the sensor stick set, the measured capacitance was composed of a basic capacitance connected in parallel with sensor stick capacitance, which was composed of lineally connected vinyl tube capacitances and the sensing part capacitance. The dielectric constant (U) of the contacted ambient moist soil located in the sensing part around the sticks interrelated with the other parameters as following equation. $$\frac{1}{C-B}=\frac{k}{U}+Z$$ where C is the output total relative capacitance, B is the hidden and fixed basic relative capacitance, k is a constant related with U, and Z is a constant for the insulating vinyl tube capacitances determined by its thickness and dielectric constant. The constant k is determined by the spacing and length of sensor sticks. The Z value is theoretically an invariable constant, but it may become considerably bigger than the determined in lab if air tube is formed on the surface of sensor sticks by some shocks on them after their installation in soil. Due to the unstability of lab Z value, it may be better to revise it after sensor stick's installation in soil and no shaking shocks should be applied on them.

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Control of Bending Behavior of Simple Beams Using CTMD (CTMD의 질량비에 따른 단순보의 휨거동 제어효과)

  • Heo, Gwang-Hee;Seo, Sang-Gu;Kim, Chung-Gil;Jeon, Seung-Gon;Kim, Min-Ki
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.25 no.6
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    • pp.12-18
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    • 2021
  • The purpose of this study is to effectively mitigate the bending displacement that occurs in the bridge due to forced vibration. We developed CTMD (Combine Tuned Mass Damper) that combines the relationship between spring and mass to control the bending behavior of simple beams. The experiment was conducted to confirm the control effect according to the change in the mass ratio of the developed CTMD. The developed CTMD is designed and manufactured so that the mass ratio can be adjusted according to the characteristics of the bridge. The maximum load of the spring applied to CTMD was fixed at 33.15 N. In order to evaluate the performance of the developed CTMD, a simple beam composed of hinges and rollers as boundary conditions was fabricated. In the experimental method, a CTMD was installed in the center of a simple beam and the deflection displacement according to the mass ratio was measured. The shaking condition was shaken at 3 Hz to induce the maximum bending behavior of the simple beam. As a result of the experiment, it was confirmed that when the optimal mass ratio was 2.1, the damping rate of the bending behavior displacement was about 71.2 %, indicating the best control effect.

Analysis of Scaling Factor applied to Lab-Scale Model for Estimating Dynamic Characteristics of Real Structures (실구조물의 동특성 파악을 위한 축소모형에 적용되는 상사비 분석)

  • Park, Gun;Yoon, Hyungchul;Kim, Sung Bo
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.25 no.1
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    • pp.59-66
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    • 2021
  • The earthquakes are the natural disasters that can cause the most serious damage to civil structures. Therefore, various studies are being conducted to secure the safety of structures against earthquakes. Most studies on the safety or mechanism of civil structures during earthquakes are being conducted based on lab scale test, because real structure tests are impossible when considering the scale of civil structures. The scaling factor proposed by Iai is mainly cited, but when applying the scaling factor proposed by Iai, there are many difficulties in selecting the structural members necessary for the production of the lab scale model. This is because when applying the scaling factor proposed by Iai, the scaling factor must be applied to the elastic modulus, which is the material property of the structure. Therefore, a new method based on Iai's 's similarity law for determining scale factor is applied in this study where the material property of real structure is same as that of lab-scale model. Through the results of this study, it is considered that the characteristics of the structure calculated through the lab scale model test can more accurately reflect the characteristics of the real structure.

Structural Performance Evaluation of Anchors for Power Equipment Electrical Cabinets Considering On-Site Installation Conditions (현장 설치 조건을 고려한 발전설비 전기 캐비닛 정착부 앵커의 구조성능 평가)

  • Lee, Sang-Moon;Jung, Woo-Young
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.43 no.6
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    • pp.709-719
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    • 2023
  • In general, most of the electrical equipment responsible for control within power plants is housed in self-standing cabinets. These cabinets are typically fixed to a slab using post-installed anchors. Although the fixation method of using post-installed anchors provides stability, there is a risk of conductor failure due to external forces, including moments. However, the performance assessment of current anchors is only evaluated through uniaxial material tests. Therefore, the primary purpose of this study is to compare the static performance of post-installed anchors, considering on-site installation conditions, with their performance in material tests and to analyze the behavioral characteristics of the anchors. While conducting experiments using actual cabinets would be ideal, practical and spatial constraints make this approach difficult. As an alternative, experiments were conducted using a test specimen consisting of a steel column and a support. As a result, the pull-out performance of anchors reflecting on-site installation conditions was measured to be about 10% higher than that observed in material tests. The trends in load reduction and the point of maximum performance for the anchors also differed. To verify the reliability of the experimental study, a 3D FEM analysis was performed, which will provide predictive information on the loads transferred to the post-installed anchors for structural performance evaluations of electrical cabinets using shaking table test in the future.

The Critical Thinking of Philosophy as a Creative Method of Science: Neurophilosophical Explication (창의적 과학방법으로서 철학의 비판적 사고: 신경철학적 해명)

  • Park, Jeyoun
    • Journal of The Korean Association For Science Education
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    • v.33 no.1
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    • pp.144-160
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    • 2013
  • This study is a proposal, which is the trial to explicate, in neurology, on how critical thinking as a creative method of sciences functions. The creative methods of sciences, even at present, are mostly the hypothetical insistences concerning with the logical processes of researches suggested from the philosophers of science; Popper, Kuhn, Hempel, or Lakatos. These insistences do excavate what process or approach can be scoped out of scientists' creativity. I call the tendency or approach of the researches, "Process Approach of Creativity (PAC)". From my view point, any PAC trial does not concern with how creative theories can actually be invented. On the other hand, this study is focused on the philosophical thinking abilities of scientists who invented new great theories. They mostly had some experiences to study philosophy while studying their science fields, thus had critical thinking abilities on their studies. From my point of view, critical thinking in philosophy raised questions as to their fundamental and basic (old) concepts and principles, and thus gave them new creative theories. I will try to explain this from the point of neurophilosophy. From the perspectives coming from "the state space theory of representation" of Paul & Patricia Churchland, the pioneers of neurophilosphy, the "creative theories" are the networks of topographic maps giving new comprehensive explanations and predictions. From these perspectives, I presuppose that the attitude of critical questioning revises the old networks of maps with back-propagation or feedback, and thus, is the generative power of searching new networks of maps. From the presupposition, I can say, it is important that scientists reflect on the basic premises in their academic branches for issuing out extraordinary creativity. The critical attitude of philosophy can make scientists construct the maps of new conceptual scheme by shaking the maps of the old basic premises. From this context, I am able to propose "Critical Thinking Approach of Creativity (CTAC)".

Direct PCR Detection of the Causal Agents, Soybean Bacterial Pustule, Xanthomonas axonopodis pv. glycines in Soybean Seeds (콩 종자에서 Xanthomonas axonopodis pv. glycines의 검출을 위한 Direct PCR 방법 개발)

  • Lee, Yong-Ju;Kang, Mi-Hyung;Noh, Tae-Hwan;Lee, Du-Ku;Lee, Geon-Hwi;Kim, Si-Ju
    • Research in Plant Disease
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    • v.15 no.2
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    • pp.83-87
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    • 2009
  • Direct Polymerase Chain Reaction (PCR) method that combines biological and enzymatic amplification of PCR targets was developed for the detection of Xanthomonas axonopodis pv. glycines on soybeen seeds without DNA isolation. Primers Xag F1 and Xag R1 were designed to specifically amplify a 401 bp fragment of the glycinecin A gene of X axonopodis pv. glycines. Xag F1 and Xag R1 were used to carry out the PCR analysis with genomic DNA from 45 different bacterial strains including phylogenetically related bacteria with X axonopodis pv. glycines, and other bacterial strains of different genus and species. The PCR assay using this set of primers were able to detect X axonopodis pv. glycines with DNA concentration as low as 200 fg and $1.8{\times}10^3$ cfu/ml. The Xag was detected from the seed samples incubated for 2 hrs with shaking and the intensity of the band was increase with the incubation time of seeds. The Direct PCR assay method without DNA isolation makes detection of X. axonopodis pv. glycines on soybean seeds easier and more sensitive than other conventional methods. The developed seed assay using direct PCR method will be useful for the specific detection of X. axonopodis pv. glycines in soybean seed samples.

Comparative Analysis of ViSCa Platform-based Mobile Payment Service with other Cases (스마트카드 가상화(ViSCa) 플랫폼 기반 모바일 결제 서비스 제안 및 타 사례와의 비교분석)

  • Lee, June-Yeop;Lee, Kyoung-Jun
    • Journal of Intelligence and Information Systems
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    • v.20 no.2
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    • pp.163-178
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    • 2014
  • Following research proposes "Virtualization of Smart Cards (ViSCa)" which is a security system that aims to provide a multi-device platform for the deployment of services that require a strong security protocol, both for the access & authentication and execution of its applications and focuses on analyzing Virtualization of Smart Cards (ViSCa) platform-based mobile payment service by comparing with other similar cases. At the present day, the appearance of new ICT, the diffusion of new user devices (such as smartphones, tablet PC, and so on) and the growth of internet penetration rate are creating many world-shaking services yet in the most of these applications' private information has to be shared, which means that security breaches and illegal access to that information are real threats that have to be solved. Also mobile payment service is, one of the innovative services, has same issues which are real threats for users because mobile payment service sometimes requires user identification, an authentication procedure and confidential data sharing. Thus, an extra layer of security is needed in their communication and execution protocols. The Virtualization of Smart Cards (ViSCa), concept is a holistic approach and centralized management for a security system that pursues to provide a ubiquitous multi-device platform for the arrangement of mobile payment services that demand a powerful security protocol, both for the access & authentication and execution of its applications. In this sense, Virtualization of Smart Cards (ViSCa) offers full interoperability and full access from any user device without any loss of security. The concept prevents possible attacks by third parties, guaranteeing the confidentiality of personal data, bank accounts or private financial information. The Virtualization of Smart Cards (ViSCa) concept is split in two different phases: the execution of the user authentication protocol on the user device and the cloud architecture that executes the secure application. Thus, the secure service access is guaranteed at anytime, anywhere and through any device supporting previously required security mechanisms. The security level is improved by using virtualization technology in the cloud. This virtualization technology is used terminal virtualization to virtualize smart card hardware and thrive to manage virtualized smart cards as a whole, through mobile cloud technology in Virtualization of Smart Cards (ViSCa) platform-based mobile payment service. This entire process is referred to as Smart Card as a Service (SCaaS). Virtualization of Smart Cards (ViSCa) platform-based mobile payment service virtualizes smart card, which is used as payment mean, and loads it in to the mobile cloud. Authentication takes place through application and helps log on to mobile cloud and chooses one of virtualized smart card as a payment method. To decide the scope of the research, which is comparing Virtualization of Smart Cards (ViSCa) platform-based mobile payment service with other similar cases, we categorized the prior researches' mobile payment service groups into distinct feature and service type. Both groups store credit card's data in the mobile device and settle the payment process at the offline market. By the location where the electronic financial transaction information (data) is stored, the groups can be categorized into two main service types. First is "App Method" which loads the data in the server connected to the application. Second "Mobile Card Method" stores its data in the Integrated Circuit (IC) chip, which holds financial transaction data, which is inbuilt in the mobile device secure element (SE). Through prior researches on accept factors of mobile payment service and its market environment, we came up with six key factors of comparative analysis which are economic, generality, security, convenience(ease of use), applicability and efficiency. Within the chosen group, we compared and analyzed the selected cases and Virtualization of Smart Cards (ViSCa) platform-based mobile payment service.

Universal Ethics and Pragmatic Pluralism (보편윤리학과 실용주의적 다원론)

  • Kwon, Su-Hyeon
    • The Journal of the Convergence on Culture Technology
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    • v.7 no.1
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    • pp.446-453
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    • 2021
  • This paper deals with two methods regarding fact and value. One is the method of H. Putnam, which is to break the boundary between fact and value and to make a world where the two have an inherent connection. The other is the method of J. Habermas, which regards fact and value as the product of an intersubjective agreement based on argumentation. Putnam, through his position of internal realism, moves from realism to pragmatism, especially by combining the rationalist tradition of Kant and Dewey's pragmatic views. Habermas also stands in the tradition of rationalism and universalism in Kant, at the same time emphasizing the practicability of truth in Hegel's tradition of historical reason. The significance of the strategy of Putnam and Habermas is that they have attempted to revive the realm of value against the strict dichotomy of facts and values and the subsequent devaluation of rationality in the realm of value. The starting point of this attempt is that the practical foundation of rationality is laid on life and practice. This could provide the room for escaping from rationality, which prioritizes only truths that reveal facts, that is, instrument-reduced rationality, the room for the revival of practical rationality through reflection on what is the purpose of life, and, in turn, the room for resisting to pass the realm of values and norms to the logic of habitual routines or customs. However, despite such common goal, there are clear limitations to Putnam's approach due to the differences in the strategies taken on facts and values. Putnam's method can demolish the whole universal framework that is the foundation where pragmatic pluralism will be fostered, eliminating the difference between the specificity of values and the universality of norms and shaking up the status of universal ethics. Therefore, Habermas' ethical theory is proposed as an alternative to establish a basis for universal ethics by relying on communication rationality and to secure the coercion of norms and blossom cultural pluralism as a diverse lifestyle based on this coercion.

Compression Sensing Technique for Efficient Structural Health Monitoring - Focusing on Optimization of CAFB and Shaking Table Test Using Kobe Seismic Waveforms (효율적인 SHM을 위한 압축센싱 기술 - Kobe 지진파형을 이용한 CAFB의 최적화 및 지진응답실험 중심으로)

  • Heo, Gwang-Hee;Lee, Chin-Ok;Seo, Sang-Gu;Jeong, Yu-Seung;Jeon, Joon-Ryong
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.24 no.2
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    • pp.23-32
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    • 2020
  • The compression sensing technology, CAFB, was developed to obtain the raw signal of the target structure by compressing it into a signal of the intended frequency range. At this point, for compression sensing, the CAFB can be optimized for various reference signals depending on the desired frequency range of the target structure. In addition, optimized CAFB should be able to efficiently compress the effective structural answers of the target structure even in sudden/dangerous conditions such as earthquakes. In this paper, the targeted frequency range for efficient structural integrity monitoring of relatively flexible structures was set below 10Hz, and the optimization method of CAFB for this purpose and the seismic response performance of CAFB in seismic conditions were evaluated experimentally. To this end, in this paper, CAFB was first optimized using Kobe seismic waveform, and embedded it in its own wireless IDAQ system. In addition, seismic response tests were conducted on two span bridges using Kobe seismic waveform. Finally, using an IDAQ system with built-in CAFB, the seismic response of the two-span bridge was wirelessly obtained, and the compression signal obtained was cross-referenced with the raw signal. From the results of the experiment, the compression signal showed excellent response performance and data compression effects in relation to the raw signal, and CAFB was able to effectively compress and sensitize the effective structural response of the structure even in seismic situations. Finally, in this paper, the optimization method of CAFB was presented to suit the intended frequency range (less than 10Hz), and CAFB proved to be an economical and efficient data compression sensing technology for instrumentation-monitoring of seismic conditions.