• Journal of the Korean Recycled Construction Resources Institute
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• v.4 no.4
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• pp.463-469
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• 2016

In this paper, compressive strength and water permeability performance for two types of crack self-healing materials such as SH-PO-0 composed of mineral admixtures(expansive agent, swelling material and crystal growth agent) and SH-PO-(5, 15, 30) blended with SH-PO-0 and phosphate additive(PO) dissolving easily calcium ion, were evaluated. The test results show that the water flow of SH-PO-0 decreased steeply at the early age although compressive strength decreased about 9% at 28 days compared with OPC. The higher PO replacement ratio is, the lower compressive strength and more improved water permeability performance is, and thus, based on such results, adequate PO replacement ratio is 15%. It is also found that the self-healing performance of SH-PO-15 was quite improved at the early ages and however, the performance of SH-PO-15 is similar to one of SH-PO-0 at long-term ages, and 28 days compressive strength of SH-PO-15 decreased about 8% compared with SH-PO-0. In addition, it is confirmed from the analysis of SEM-EDS that calcium ions of SH-PO-15 were crystallized more than those of SH-PO-0.

• The Korean Journal of Emergency Medical Services
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• v.16 no.2
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• pp.43-51
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• 2012

Purpose : We try to disclose how much the keywords of the papers from the Korean Journal of Emergency Medical Services with Medical Subject Headings(MeSH) terminologies and to understand the major subjects of the recent emergency medical technology research in Korea from keywords. Methods : We analyzed keywords from 524 articles of the Korean Journal of Emergency Medical Services that were published between 2005 and 2011. We investigated frequently used keywords and what percentages of keywords agree with MeSH terms using the MeSH browser. Results : There were on average 3.2 keywords per article. The most frequent key words were AED, Attitude, Cardiopulmonary Resuscitation, CPR, EMT, EMT students, External Defibrillator, Job satisfaction, Knowledge, 119 EMT in order. The number of terms in precise agreement with MeSH headings was 101(19.3%); 327 terms(62.4%) were not found in the MeSH browser and 96 terms(18.3%) partially matched MeSH terms. Conclusion : Many keywords used in the Korean Journal of Emergency Medical Services did not agree with MeSH terms. We conclude that contribution rules should be using MeSH terms and authors should be educated in the proper use of MeSH terms in their research and subsequent publication.

• Toxicological Research
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• v.32 no.3
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• pp.239-243
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• 2016

Propolis is a multicomponent, active, complex resinous substance collected by honeybees from a variety of plant sources. We have studied the effect of propolis on neurite outgrowth of SH-SY5Y human neuroblastoma cells induced to differentiate by all-trans-retinoic acid (RA). Propolis, at a concentration of $3{\mu}g/mL$, had no significant effect on the viability of differentiating SH-SY5Y cells. However, the neurite outgrowth of the differentiating SH-SY5Y cells treated with propolis ($0.3{\sim}3{\mu}g/mL$) for 48 hr was significantly inhibited in a dose-dependent manner. Treatment of RA-stimulated differentiating SH-SY5Y cells with 0.3 to $3{\mu}g/mL$ propolis resulted in decreased level of transglutaminase and 43-kDa growth-associated protein (GAP-43) in a dose-dependent manner. The results indicate that propolis is able to inhibit neurite outgrowth of differentiating SH-SY5Y cells.

• Journal of Korean Ophthalmic Optics Society
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• v.21 no.2
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• pp.83-90
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• 2016

Purpose: The purpose of this study is to compare and analyze keywords of articles in the Korean Ophthalmic Optics Society to MeSH (Medical Subject Headings) terms. The study hopes to enhance the understanding and usage of MeSH and give fundamental information to the Korean Ophthalmic Optics Society in advance. Methods: A total of 1952 keywords from 409 informative articles published from 2004, Vol 9(1) to 2016, Vol 21(1) were compared with MeSH terms according to the criteria of complete coincidence, incomplete coincidence and complete incoincidence. Results: 439 keywords (22.4%) were completely coincident with MeSH terms, 815 keywords (41.8%) were incompletely coincident with MeSH terms and 693 keywords (35.5%) were completely incoincident with MeSH terms. The most used keyword in MeSH terms is in the order of Myopia, Astigmatism and visual acuity. For the incompletely coincident keywords Refractive error, Soft contact lens, and Phoria were used the most. Finally, the most used keywords in the category of completely incoincident were Accommodative lag and Pseudomonas aeruginosa. Conclusions: It is highly recommended that MeSH terms are selected as controlled keywords to increase usage of searced Korean Ophthalmic Optics Society articles in MEDLINE.

• BMB Reports
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• v.31 no.4
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• pp.370-376
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• 1998

Src-Homology 2 (SH2) domains have a capacity to bind phosphotyrosine-containing sequence context and play essential roles in various cellular signaling pathways. Due to the specific nature of the binding between SH2 domains and their counterpart proteins, inhibitors of SID domain binding have drawn extensive attention as a potential candidate for therapeutic agents. Here, we describe the binding assay system to screen for the ligands or blockers of the SH2 domains with an emphasis on the $p56^{lck}$ SH2 domain. In our assay system, SID domains expressed and purified as fusion proteins to Glutathione-S-transferase (GST) were covalently attached to 96-well microtitre plates through amide bond formation, which were subsequently allowed to bind the biotinylated phosphotyrosine (pY)containing synthetic pep tides. The binding of biotinylated pY peptides was detected by the horseradish peroxidase (HRP)-conjugated streptavidin. Using the various combinations of SH2 domain-pY peptides, we observed that: (1) The binding of pY-peptides to its counterpart SH2 domain is concentration-dependent and saturable; (2) The binding is highly specific for a particular combination of SH2 domain-pY peptide pair; and (3) The binding of Lck SH2-cognate pY-peptides is specifically competed by the nonbiotinylated peptides with expected relative affinity. These results indicate that the established assay system detects the SH2-pY peptide interaction with reproducible sensitivity and specificity and is suitable for screening the specific inhibitors of $p56^{lck}$ SH2 function.

• Journal of Sasang Constitutional Medicine
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• v.25 no.1
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• pp.34-42
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• 2013

Objectives The purpose of this study was analyzing the equality between the MeSH terms and the keyword used in the papers published in Journal of Sasang Constitutional Medicine and investigating how to use an appropriate MeSH terms as keyword in the papers. Methods A total of 704 keyword used in 177 papers published from 2009 to 2012 in Journal of Sasang Constitutional Medicine were analyzed to investigate the equality between the keyword and the MeSH terms. The collected data was analyzed using SPSS 17.0 software for frequency analysis. Results Among the 704 keyword, 107 keyword(15.2%) was perfectly matched with the MeSH terms. 64 keyword(9.1%) showed partial difference was with the MeSH terms, and 11 keyword(1.7%) showed partial difference was with the Entry terms. 127 keyword(18.0%) were included in the exception item due to the nature of journal, and 395 keyword(56.1%) were not perfectly matched with the MeSH terms. In the yearly analysis result, the number of papers that keyword and MeSH terms perfectly matched was not significant changed, however the number of papers that keyword and MeSH terms did not matched was continuously increased, which clearly indicate use of MeSH terms as the keyword of the papers published in the journal of Sasang constitution medicine is insufficient. Conclusions The papers published in journal of Sasang constitutional medicine need to be cited in various fields and the paper's finding need to affect in other studies for the development of Korean medicine and Sasang constitutional medicine. The use of proper keyword aligned with the international standards is necessary to accomplish the globalization of them.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.2
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• pp.206-213
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• 2011

An experiment was conducted to evaluate the effect of replacing barley and soybean meal in finishing diets with sesame hull (SH) on growth performance, digestibility, and carcass characteristics of Black goat kids. Twenty-one Black goat kids were assigned randomly to 0%, 10%, or 20% SH diets (7/diet). The study lasted for 63 d. Intakes of dry matter (DM), organic matter (OM), and crude protein (CP) of kids fed the 10% diet were greater (p<0.05) than for the 0% and 20% SH diets (p<0.10). Neutral detergent fiber (NDF) and acid detergent fiber (ADF) intakes were highest (p<0.05) for kids fed the 10% SH diet when compared to 0% and 20% SH diets. Ether extract (EE) intake was greater (p<0.05) for kids fed 10% and 20% SH diets when compared to 0% SH. Digestibilities of DM, OM, CP, NDF, and ADF were similar among all diets. However, sesame hull inclusion increased (p<0.05) EE digestibility. Final body weight, total gain, average daily gain (ADG), and feed conversion ratio were comparable among all diets. Cost of gain was lower (p<0.05) in kids fed 20% SH than the 0% SH diet, while there was no difference between the 10% SH diet and the other two diets. Dressing percentage, hot and cold carcass weights, non-carcass components, carcass cut weights, and meat quality parameters were not influenced by SH. Total fat percentage in the leg was greater (p<0.05) on 0% SH than on the 10% SH diet. Results of this study demonstrated that inclusion of sesame hull at levels of 10 or 20% did not influence the performance of Black goat kids, but the cost of gain was lower.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.45 no.6
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• pp.600-605
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• 2012

In this study, we investigated the mechanism of inhibition of pathogenic bacteria by Pediococcus pentosaceus strain SH-10 isolated from hard Clam Meretrix meretrix sikhae. When P. pentosaceus SH-10 was co-cultured in MRS broth with pathogenic bacteria, including Bacillus cereus, Listeria monocytogenes, Salmonella choleraesuis and Staphyloccus aureus, no viable pathogenic cells were detected after 18 h of incubation. However, pediocin or a pediocin-like bacteriocin was not detected in cultures of P. pentosaceus SH-10 by the agar diffusion method. Organic acids were produced in MRS broth in proportion to the incubation time of P. pentosaceus SH-10. These results indicate that P. pentosaceus SH-10 inhibited the growth of pathogenic bacteria by lowering the pH of the growth medium through the production of organic acids, including sodium lactate, sodium acetate, and sodium citrate.

• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.1
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• pp.1-6
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• 2004

The human chemokine, the short version of leukotactin-1(shLkn-1;molecular weight=7.2 kD and 66 amino acids), was expressed and secreted into a culture medium using the me-thylotrophic yeast, Pichia pastoris. The recombinant shLkn-1 was purified from the culture supernatant using a simple two-step procedure consisting of cation exchange and reverse phase chromatography(RPC), in which shLkn-1 was highly purified (99.5%) with a high recovery yield of 82.7%. The C-terminal truncated derivative of shLkn-1 was found in the supernatant and was separated by RPC. The physicochemical properties of the purified shLkn-1 were verified to be the same as expected. The biological activity of the purified recombinant shLkn-1 was also quantified using a chemotaxis assay. It was observed that the recombinant shLkn-1 had the maximum migration activity at a concentration of 10nM, as potent as MIP-1${\alpha}$.

• Microbiology and Biotechnology Letters
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• v.30 no.4
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• pp.298-304
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• 2002

A family shuffling associating PCR-based and in vitro recombination and expression in Escherichia coli cdd mutant was carried out. Two cdd genes encoding cytidine deaminases (CDase) from thermophilic Bacillus caldolyticus and B. stearothermophilus were shuffled. Around 150 viable mutant colonies screened on AB minimal medium without uracil by E. coli cdd complementation were selected for cytidine deaminase assay and 4 candidates (SH1067, SH1077, SH1086, and SH1118) were chosen for the detailed study. The nucleotide sequence analyses of 4 selected mutants revealed that they have several point mutations and recombinations. Surprisingly, the SH 1067 showed 770 fold more specific CDase activity at $80^{\circ}C$ than that of T101 from parental B. stearothermophilus.