• Journal of Korean Society for Atmospheric Environment
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• v.21 no.5
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• pp.525-535
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• 2005

Using a three-dimensional computational fluid dynamics (CFD) model with the $k-{\varepsilon}$ turbulence closure scheme based on the renormalization group theory, flow regimes in urban street canyons are classified according to the building and street aspect ratios. The transition between skimming flow (SF) and wake interference flow (WIF) is determined with the size of double-eddy circulation generated behind the upwind building. The transition between WIF and isolated roughness flow (IRF) is determined with the flow reattachment distance from the upwind building. The critical aspect ratios at which the flow transition occurs are found and compared with those in previous studies. The results show that the flow-regime classification method used in this study is quite reasonable and that the values of the critical aspect ratios are generally consistent with those in fluid experiments or large-eddy simulation. The regression equation describing a relation between the building and street aspect ratios at the flow-regime transition is presented.

• Journal of Oral Medicine and Pain
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• v.39 no.1
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• pp.15-21
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• 2014

Purpose: The purpose of this study was to investigate the relationship between personality type and oral hygiene controllability. Methods: Four hundred eighty-two college students in Gyeonggi-do completed the Myers-Briggs type indicator (MBTI) and a questionnaire and collected data were analyzed by SAS 9.2 program. Results: Compared to extroverted subjects, a significantly increased percentage of introverted subjects demonstrated oral malodor and stress (p<0.05). Tongue coating and stress seemed to occur the most frequently in intuition-feeling (NF) type, while oral malodor seemed to occur the most frequently in sensation-feeling (SF) type among four fuctional types. Tongue coating and stress seemed to occur the most frequently in NF type, while oral malodor seemed to occur the most frequently in sensation-perceiving (SP) type among four temperaments. Significantly increased mean scales of tongue scraping index and oral hygiene controllability index were found for extroverts (p<0.05). Mean scales of tooth brushing index and oral hygiene controllability index appeared to be the highest in NF type among four fuctional types. Mean scales of tongue scraping index and oral hygiene controllability index appeared to be the highest in sensation-judging (SJ) type among four temperaments. Conclusions: Oral hygiene controllability was associated with personality type and it is necessary to develop oral health education program considering personality type.

• Biomedical Science Letters
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• v.18 no.2
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• pp.160-164
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• 2012

The baculovirus/insect cell expression system is of great value for the large-scale production of normal and mutant mammalian passive glucose-transport proteins heterologously for structural and functional studies. In most mammalian cells that express HepG2, this transporter isoform is predominantly located at the cell surface. However, it had been reported that heterologous expression of other membrane proteins using the baculovirus system induced highly vacuolated cytoplasmic membranes. Therefore, how a cell responds to the synthesis of large amounts of a glycoprotein could be an interesting area for investigation. In order to examine the subcellular location of the human HepG2 transport proteins when expressed in insect cells, immunofluorescence studies were carried out. Insect cells were infected with the recombinant baculovirus AcNPVHIS-GT or with wild-type virus at a MOI of 5, or were not exposed to viral infection. A high level of fluorescence displayed in cells infected with the recombinant virus indicated that transporters are expressed abundantly and present on the surface of infected Sf21 cells. The evidence for the specificity of the immunostaining was strengthened by the negative results shown in the negative controls. Distribution of the transporter protein expressed in insect cells was further revealed by making a series of optical sections through an AcNPVHIS-GT-infected cell using a confocal microscope, which permits optical sectioning of cell sample. These sections displayed intense cytoplasmic immunofluorecence surrounding the region occupied by the enlarged nucleus, indicating that the expressed protein was present not only at the cell surface but also throughout the cytoplasmic membranous structures.

• Journal of Microbiology and Biotechnology
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• v.25 no.11
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• pp.1960-1965
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• 2015

Rabbit hemorrhagic disease virus (RHDV) is highly contagious and often causes fatal disease that affects both wild and domestic rabbits of the species Oryctolagus cuniculus. A highly pathogenic RHDV variant (RHDVa) has been circulation in the Korean rabbit population since 2007 and has a devastating effect on the rabbit industry in Korea. A highly pathogenic RHDVa was isolated from naturally infected rabbits, and the gene encoding the VP60 protein was cloned into a baculovirus transfer vector and expressed in insect cells. The hemagglutination titer of the Sf-9 cell lysate infected with recombinant VP60 baculovirus was 131,072 units/50 μl and of the supernatant 4,096 units/50 μl. Guinea pigs immunized twice intramuscularly with a trial inactivated RHDVa vaccine containing recombinant VP60 contained 2,152 hemagglutination inhibition (HI) geometric mean titers. The 8-week-old white rabbits inoculated with one vaccine dose were challenged with a lethal RHDVa 21 days later and showed 100% survival rates. The recombinant VP60 protein expressed in a baculovirus system induced high HI titers in guinea pigs and rendered complete protection, which led to the development of a novel inactivated RHDVa vaccine.

• Journal of Korean Academy of Nursing
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• v.36 no.3
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• pp.449-456
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• 2006

Purpose: The purpose of this study was to investigate the influences of health perception, body image and sexual function on depression according to the menopausal stage in Korean middle aged women. Methods: Subjects were 182 perimenopausal and postmenopausal women who had not received hormonal replacement therapy. A five-item General HealthShort Form (SF-36) Health Survey Questionnaire (Ware & Sherbourne, 1992) was used to measure health perception. Body image was measured Semantic Differential scale, CES-D was used to measure the level of depression, and sexual function was measured by FSFI. Results: The subjects rated their health as 'moderate', their body image as 'moderate, and level of depression as 'high'. However, there were no significant differences in health perception, body image, and depression between perimenopasual and postmenopausal women. Postmenopausal women experienced lower levels of sexual function than perimenopausal women in sexual arousal, lubrication, orgasm, satisfaction, and pain. In stepwise regression analysis, 21.7% of variance in depression was shown to be perceived health and body image in postmenopausal women. However, in perimenopausal women, perceived health and sexual function explained 34.5% of variance in depression. Conclusion: These findings indicate that nurses must consider the menopasusal stage when counseling their patients.

• Journal of Korean Academy of Rural Health Nursing
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• v.10 no.1
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• pp.17-29
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• 2015

Purpose: This study was done to examine factors affecting quality of life among community-dwelling elderly women who live in rural areas. Methods: The design of this research was cross-sectional descriptive study. The participants were 92 community-dwelling women aged 65 or older. Data were collected from November 1 to 15, 2013. ADL (Kart's Index), GDS-SF (Geriatric Depression Scale Short Form) and GQOL (Geriatric Quality of Life Scale) were used to measure variables. Data were collected using self-administered questionnaires and analyzed using descriptive statistics, Spearman's coefficient and stepwise multiple regression. Results: The participants' mean age was 77.5, and 85.9% had elementary school graduation or less education, and for 64.1%, their economic status was low. Spearman's rho coefficient analysis found that QOL was significantly associated with depression (r=-.72, p<.001), perceived health (r=.58, p<.001), regular exercise (r=.47, p<.001), education level (r=.29, p=.005), and ADL (r=-.21, p=.043). Multiple regression analysis showed that 65.9% of their QOL was explained by depression (${\beta}=-.72$), perceived health (${\beta}=.24$), ADL (${\beta}=-.16$), exercise (${\beta}=.22$) and number of diseases (${\beta}=.19$). Conclusion: These results indicate that older women who live rural areas need support for ADL, and prevention of depression to improve their quality of life.

• Journal of Korean Academy of Nursing
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• v.37 no.3
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• pp.324-333
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• 2007

Purpose: The purpose of this study was to develop and evaluate a quality of life scale for Korean patients with cancer (C-QOL). Methods: The C-QOL was developed and validated as follows, item generation, pilot study, and psychometric tests. A total of 337 patients diagnosed with stomach, liver, lung, colon, breast, or cervix cancer were recruited. The patients were asked to complete the preliminary questionnaire comprising the content-validated items, the SF-36, and the ECOG performance status. The obtained data was analyzed using descriptive statistics, factor analysis, multidimensional scaling (MDS), multitrait/multi-item matrix, ANOVA, t-test, and Cronbach's alpha. Results: Preliminarily twenty-six items were generated through content validity and a pilot study. Factor analysis and MDS extracted a total of 21 items with a 5-point Likert-type scale (C-QOL). The C-QOL included five subscales: physical status (6 items), emotional status (6 items), social function (3 items), concern status (2 items), and coping function (4 items). The C-QOL established content validity, construct validity, item convergent and discriminant validity, known-groups validity, reliability, and sensitivity. Conclusion: The Newly developed C-QOL is an easily applicable instrument which established psychometric properties and reflected Korean culture. It is recommended for further study to examine the responsiveness of the C-QOL using a longitudinal research design.

• The Journal of Korean Physical Therapy
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• v.21 no.4
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• pp.89-96
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• 2009

Purpose: This study examined the effects of manual lymph drainage (MLD) on edema and the quality of life (QOL) of lymphedema patients in the maintenance phase. Method: Forty five lymphedema patients, who had completed intensive decongestive therapy, were enrolled in this study. All subjects were assigned randomly to two groups: Experimental (MLD) group (n=23) and control (self-MLD) group (n=22). MLD by a specialist was applied with a comfortable pressure for 40~60 minutes in the experimental group and self-MLD was carried out by the control group 5 times a week for 2 weeks. The % edema and a SF-36 questionnaire were used to measure the decrease in edema and the QOL. A paired t-test was used to compare the period, and an independent T-test was used to compare experiment and control groups. Result: The % edema was significantly lower in the experimental group after MLD (p<0.05). The physical, vitality, and mental health scores of the experimental group was significantly higher than those of the control group (p<0.05). Conclusion: TMLD by a specialist in the maintenance phase is effective in reducing the % edema and enhancing the QOL of lymphedema patients. Further studies will be needed to determine if there are differences in the effects of many types of treatment methods in the lymphedema treatment.

• International Journal of Industrial Entomology and Biomaterials
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• v.21 no.2
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• pp.175-179
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• 2010

Silk fibroin membrane was prepared and examined to know the feasibility of SF membrane as guided bone regeneration. The morphology of silk membrane was flat and smooth surface. The conformation of silk fibroin was $\beta$-sheet structure. When the silk membrane was applied on the rat calvarial defect model, it showed significantly higher new bone formation than uncovered control in histomorphometric analysis. The silk membrane was covered by thin fibrotic tissue and there was not observed any inflammatory cells infiltration. In conclusion, silk fibroin membrane could be useful materials for guided bone regeneration.

• Journal of Microbiology and Biotechnology
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• v.18 no.5
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• pp.964-967
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• 2008

Two structural protein genes, VP19 and VP466, of white spot syndrome virus (WSSV) were cloned and expressed in Sf21 insect cells using a baculovirus expression system for the development of injection and oral feeding vaccines against WSSV for shrimps. The cumulative mortalities of the shrimps vaccinated by the injection of rVP19 and rVP466 at 15 days after the challenge with WSSV were 50.2% and 51.8%, respectively. For the vaccination by oral feeding of rVP19 and rVP466, the cumulative mortalities were 49.2% and 89.2%, respectively. These results show that protection against WSSV can be generated in the shrimp, using the viral structural protein as a protein vaccine.