International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Silk Fibroin Membrane as Guided Bone Regeneration in Rat Calvarial Defects

  • Kweon, Hae-Yong (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Seong-Gon (Department of Oral and Maxillofacial Surgery, Gangneung-Wonju National University) ;
  • An, Jin-Hee (Department of Prosthodontics, Hallym Sacred Heart Hospital) ;
  • Shim, Hye-Won (Department of Prosthodontics, Hallym Sacred Heart Hospital) ;
  • Yang, Byoung-Eun (Department of Prosthodontics, Hallym Sacred Heart Hospital) ;
  • Kim, Jwa-Young (Department of Prosthodontics, Hallym Sacred Heart Hospital) ;
  • Jo, You-Young (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Yeo, Joo-Hong (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA) ;
  • Lee, Kwang-Gill (Sericultural & Apicultural Materials Division, National Academy of Agricultural Science, RDA)
  • Received : 2010.11.14
  • Accepted : 2010.11.25
  • Published : 2010.12.31
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Abstract

Silk fibroin membrane was prepared and examined to know the feasibility of SF membrane as guided bone regeneration. The morphology of silk membrane was flat and smooth surface. The conformation of silk fibroin was $\beta$-sheet structure. When the silk membrane was applied on the rat calvarial defect model, it showed significantly higher new bone formation than uncovered control in histomorphometric analysis. The silk membrane was covered by thin fibrotic tissue and there was not observed any inflammatory cells infiltration. In conclusion, silk fibroin membrane could be useful materials for guided bone regeneration.

Keywords

References

  1. Fowler EB, Breault LG, Rebitski G (2000) Ridge preservation utilizing an acellular dermal allograft and demineralized freeze-dried bone allograft: Part I, A report of 2 cases. J Periodontol 71, 1353-1359. https://doi.org/10.1902/jop.2000.71.8.1353
  2. Gotfredsen K, Nimb L, Buse D, Hjortin-Hansen E (1993) Evaluation of guided bone regeneration around implants placed into fresh extraction sockets: an experimental study in dogs. J Oral Maxillofac Surg 51, 879-884. https://doi.org/10.1016/S0278-2391(10)80108-9
  3. Gottlow J (1993) Guided tissue regeneration using bioresorbable and non-resorbable devices: initial healing and longterm results. J Periodontol 64, 1157-1165. https://doi.org/10.1902/jop.1993.64.11s.1157
  4. Hammerle CH, Jung RE (2003) Bone augmentation by means of barrier membranes. Periodontol 33, 36-53. https://doi.org/10.1046/j.0906-6713.2003.03304.x
  5. Hanawa T, Watanabe A, Tsuchiya T, Ikoma R, Hidaka M, Sugihara M (1995) New oral dosage form for elderly patients. II. Release behavior of benfotiamine from silk fibroin gel. Chem Pharm Bull, 43, 872-876. https://doi.org/10.1248/cpb.43.872
  6. Karring T, Nyman S, Gottlow J, Laurell L (1993) Development of the biological concept of guided tissue regeneration-animal and human studies. Periodontol 1, 26-35. https://doi.org/10.1111/j.1600-0757.1993.tb00204.x
  7. Kim KH, Jeong L, Park HN, Shin SY, Park WH, Lee SC, Kim TI, Park YJ, Seol YJ, Lee YM, Ku Y, Rhyu IC, Han SB, Chung CP (2005) Biological efficacy of silk fibroin nanofiber membranes for guided bone regeneration. J Biotechnol 120, 327-339. https://doi.org/10.1016/j.jbiotec.2005.06.033
  8. Kim J, Choi J, Kim A, Kim S, Kweon H, Jo Y, Yeo J (2010) Low molecular weight Silk Fibroin increases alkaline phosphatase and Type I collagen expression in MG63 Cells. BMB Rep 43, 52-56. https://doi.org/10.5483/BMBRep.2010.43.1.052
  9. Kim J, Kim CH, Park CH, Seo J, Kweon H, Kang SW, Lee KG (2010) Comparison of methods for the repair of acute tympanic membrane perforations: silk patch vs. paper patch. Wound Repair and Regeneration 18, 132-138. https://doi.org/10.1111/j.1524-475X.2009.00565.x
  10. Kweon HY, Ha HC, Um IC, Park YH (2001) Physical properties of silk fibroin/chitosan blend films. J Appl Polym Sci 80, 928-934. https://doi.org/10.1002/app.1172
  11. Kweon HY, Yeo J, Lee K, Lee HC, Na HS, Won YH, Cho CS (2008) Semi-interpenetrating polymer networks composed of silk fibroin and poly(ethylene glycol) for wound dressing. Biomedical Materials 3, 34115. https://doi.org/10.1088/1748-6041/3/3/034115
  12. Magoshi J, Magoshi Y, Nakamur S (1985) Crystallization, liquid crystal, and fiber formation of silk fibroin. J Appl Polym Sci: Appl Polym Symp 41, 187-204.
  13. Minoura N, Tsukada M, Nagura M (1990) Fine structure and oxygen permeability of silk fibroin membrane treated with methanol. Polymer 31, 265-269. https://doi.org/10.1016/0032-3861(90)90117-H
  14. Minoura N, Aiba S, Gotoh Y, Tsukada M, Imai Y (1995) Attachment and growth of cultured fibroblast cells on silk protein matrices. J Biomed Mater Res 29, 1215-1221 https://doi.org/10.1002/jbm.820291008
  15. Sakabe H, Ito H, Miyamoto T, Noishiki Y, Ha WS (1989) In vivo blood compatibility of regenerated silk fibroin. Sen-I Gakkaishi, 45, 487-490. https://doi.org/10.2115/fiber.45.11_487
  16. Santin M, Motta A, Freddi G, Cannas M (1999) In vitro evaluation of the inflammatory potential of the silk fibron J Biomed Mater Res 46, 382-389. https://doi.org/10.1002/(SICI)1097-4636(19990905)46:3<382::AID-JBM11>3.0.CO;2-R
  17. Sofia S, McCarthy MB, Gronowiza G, Kaplan DL (2001) Functionalized silk-based biomaterials for bone formation. J Biomed Mater Res 54, 139-148. https://doi.org/10.1002/1097-4636(200101)54:1<139::AID-JBM17>3.0.CO;2-7
  18. Tsukada M, Gotoh Y, Nagura M, Minoura N, Kasai N, Freddi G (1994) Structural changes of silk fibroin membranes induced by immersion in methanol aqueous solution. J Polym Sci Polym Phys Ed 32, 961-968. https://doi.org/10.1002/polb.1994.090320519
  19. Warrer K, Karring T, Nyman S, Gogolewski S (1992) Guided tissue regeneration using biodegradable membranes of polylactic acid or polyurethane. J Clin Periodontol 19, 633-640. https://doi.org/10.1111/j.1600-051X.1992.tb01711.x
  20. Yamada H, Igarashi Y, Takasu Y, Saito H, Tsubouchi K (2004) Identification of fibroin-derived peptides enhancing the proliferation of cultured human skin fibroblasts. Biomaterials 25, 467-472. https://doi.org/10.1016/S0142-9612(03)00540-4
  21. Yeo JH, Lee KG, Lee HS, Lee YW, Kim SY (2000) Studies on PVA/chitosan/fibroin blend sponge sheets: preparation and wound healing effects in Rats. Int J Indust Entomol 1, 59-64.