• Title/Summary/Keyword: Sex life

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Sex Identification of Bovine Meat Using Male Specific SRY and ZFY Genes (웅성 특이적 SRY 및 ZFY 유전자를 이용한 쇠고기 성(性) 판별)

  • Shin, Sung-Chul;Chung, Ku-Young;Chung, Eui-Ryong
    • Food Science of Animal Resources
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    • v.27 no.3
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    • pp.351-356
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    • 2007
  • The objective of this study was to develop a rapid and reliable method for the sex determination of beef using the PCR(polymerase chain reaction) technique. We have used two bovine sex determining genes, SRY and ZFY, on the Y-chromosome to identify the sex of Hanwoo and Holstein beet. We attempted to amplify 1,348 bp and 979 bp fragments from male and female genomic DNA corresponding to the SRY and ZFY genes, respectively, using male specific primers. The amplified PCR products were separated by electrophoresis in a 1.5% agarose gel to detect a male specific DNA band. When DNA from male beef was amplified with primers specific for the SRY gene, a DNA band of 1,348 bp was present in all of the male samples, but absent from all of the female samples. Also, when DNA from male beef was amplified with primers specific for the ZFY gene, a DNA band of 979 bp was observed in all of the male samples, but absent from all female samples. In conclusion, the bovine SRY and ZFY genes are typically found only in male beef. For the practical application of this method for the sexing of commercial beef at the processing and marketing stages after slaughter. a total of 350 beef samples collected randomly from local markets were analyzed for sex determination. The proportions of male and female samples were 252 (72%) and 98 (28%), respectively. Therefore. the SRY and ZFY genes. which are specific for the Y-chromosome, may be useful sex-diagnostic DNA markers to distinguish male meat from female meat.

Molecular Sex Determination Using Sexual Dimorphisms between ZFX and ZFY Genes in Korean Hares(Lepus coreanus Thomas) (한국멧토끼 ZFX와 ZFY 유전자의 성별 이형성과 분자 성판별)

  • Han, Sang-Hyun;Cho, In-Cheol;Lee, Sung-Soo;Oh, Moon-You;Oh, Hong-Shik
    • Journal of Life Science
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    • v.17 no.3 s.83
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    • pp.402-406
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    • 2007
  • This study was performed to develop the molecular marker for sex determination of hare (Lepus coreanus) distributed in Korea which focused on sexual dimorphism between X and Y chromosomal homologous genes, zinc finger-X (ZFX) and -Y (ZFY). The intron 7 regions of ZFX and ZFY genes exhibited differential amplification patterns between male and female hares. The lengths of intron 7 region of ZFX and ZFY genes were 538 and 233-bp, respectively. Especially, the ZFX intron 7 contained a repetitive sequence identified as member of RNA-mediated transposable elements which was similar to CSINE2 commonly found in the rabbit genome. However, it was not present in intron 7 of ZFY gene. The molecular sex typing by polymerase chain reaction (PCR) was also carried out to determine the sex of hare based on difference in lengths between the intron 7 regions of ZFX and ZFY genes. All DNA samples tested had common band amplified from ZFX. However, the male hare DNAs had two distinct bands which amplified from ZFX and ZFY genes, respectively. The results from ZFX-ZFY PCR sex typing were identical to those from phenotypic investigation and from amplification patterns using male-specific sex determining region Y (SRY) gene as well. Finally, this study suggested that the sexual dimorphism between intron 7 regions of ZFX and ZFY could be useful genetic marker to determine sex of hare.

A Study on Image of Functional Food and Meal Patterns of Health Behavior (기능성 식품에 대한 인식과 건강에 관여하는 식생활에 관한 연구 -대구지역을 중심으로-)

  • 김미향
    • Journal of the East Asian Society of Dietary Life
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    • v.11 no.2
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    • pp.151-159
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    • 2001
  • This study was conducted to determine the image of functional foods and the meal patterns for health behavior of 242 persons in Daegu area. Anthropometric data showed that female and male were 38, and 204 respectively. The most BMI group was standard group(55.4% )The most eaten functional foods were Lactobicilli goods while the lowest functional foods were Taurine goods. The highest functional food image score on the basis of Likert's 5 scales was 3.89(expensive) and the lowest score was 2.98 (experienced in eating functional food). Accepted food groups were significantly associated with eating rate of protein food and age(p < 0.01),eating rate of cabohydrate foods and sex(p < 0.05), eating rate of mineral foods and monthly average income, eating rate of vitamin foods and sex.

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A Study on the Ecologically Concerned Consumer Skill and Related Variables.; In Ulsan Area (환경문제에 대한 소비자기능과 관련변수에 대한 연구-울산지역을 중심으로-)

  • 서정희
    • Journal of Families and Better Life
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    • v.9 no.2
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    • pp.93-101
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    • 1991
  • The purpose of this research is to offer the basic data for the consumer environmental education by investigation socio-demographic variables, ecologically concerned consumer skill and interrelationship between them. Research was conducted during April for 588 samples who lives in Ulsan by using the questionnaires. The major findings of the research were summerized as follows. 1) The level of ecologically concerned consumer skill was low. 2) Sex, monthly family income, education and life styles have turned out to be significant influence on the level of ecologically concerned consumer skill. 3) The explaining power of life styles was greater than the socio-demographic variables such as sex, monthly family income and education.

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Formal Synthesis of Sex Pheromone of Gypsy Moth (+)-Disparlure from L-(+)-Tartaric Acid

  • Gi Baek Gwon;Hang Soo Kim;Jae Won Park;Jong Soo Choi;Kyung Oh Doh;Kyung Jin Kim;Young Bae, Seu
    • Journal of the Korean Chemical Society
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    • v.68 no.3
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    • pp.131-134
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    • 2024
  • A simple strategy for the formal synthesis of the sex pheromone of gypsy moth (+)-disparlure from L-(+)-tartaric acid is described herein. The key steps include the mono-esterification and regioselective ring-opening of an epoxide using a Grignard reagent. The strategy of conferring asymmetry using 2-butanone enables mono-esterification in high yield and reduces the number of steps. Subsequently, (+)-disparlure is synthesized via the regioselective ring opening of the epoxide.

Disorder of Sexual Development in a Cocker Spaniel Dog: Sry-negative XX Sex Reversal (코커 스파니엘 개에서 발생한 성-발달장애: Sry-negative XX 성전환)

  • Lee, Sung-Lim;Maeng, Geun-Ho;Kim, Na-Hyun;Park, Ki-Tae;Lee, Hee-Chun;Lee, Jae-Hoon
    • Journal of Veterinary Clinics
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    • v.29 no.5
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    • pp.422-426
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    • 2012
  • A 3-year-old Cocker spaniel was presented for evaluation because of a mass protruding from the vulva. This mass confirmed the presence of an internal bone in clitoris on the radiography. We recommended to the owner of the surgical removal of the gonads and uterus as well as the excision of the enlarged clitoris and os clitoris. The dog had gonads in the region of where the ovaries were located and presented what appeared to be a bicornate uterus and ductus deferens. Histological analysis revealed a morphologically normal uterus and two testes with their epididymis and ductus deferens. Karyotyping revealed a normal female chromosome constitution (78, XX), and polymerase chain reaction (PCR) identified the absence of Sry. Based on these findings, the patient was diagnosed with Sry-negative XX sex reversal.

A Comparison of the Sexual Concerns of Men and Women with Spinal Cord Injuries (남녀 척수장애인의 성적관심, 성적적응과 관련요인 비교 연구)

  • Kang, Hyun-Sook;Suh, Yeon-Ok
    • The Korean Journal of Rehabilitation Nursing
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    • v.4 no.2
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    • pp.198-206
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    • 2001
  • The purpose of this study is to compare sexual concerns and adjustment between men and women with spinal cord injuries. A total of 108 persons (67 men and 41 women), registered to the Spinal Cord Injury Organization or admitted at rehabilitation unit from February 1999 to February 2000, were selected as subjects of the study. Participants answered the questionnaires concerning importance of life events, sexual concern, sexual adjustment, relationship with a sex partner, and depression. The results are as follows : 1) There were no significant differences between men and women in the general characteristics except sexual dysfunction. 2) With respect to eleven different life areas, sex life ranked the fifth among men, whereas it occupied the lowest position for women among the 11 areas. However economic status was the most highly-ranked for both sex. 3) Men took much interest in the "methods and techniques for achieving sexual satisfaction", "Helping a partner cope emotionally with limitations on sexual activity", whereas women were interested in "Helping a partner cope emotionally with limitations on sexual activity" and "Ability to have children". 4) The means of sexual adjustment, relationship with a sex partner, and depression among men were higher than women. But there was no significant difference between two groups. As a result, the study suggests that there are no significant differences in the general characteristics between men and women with spinal cord injuries except sexual dysfunction. Men evaluated the sexual events highly compared to women, and differences were also shown in terms of sexual concerns between men and women. Therefore differential approaches to sexual rehabilitation are required; in both socio-psychological and physical aspects for men and particularly in socio-psychological respect for women.

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A Study about Bisexuality in Current Fashion-Basis on a Representative Designers and their Characteristics in the each period- (현대패션에 나타난 양성적 복식에 관한 연구-시대별 대표적 디자이너와 그 특성을 중심으로-)

  • 채금석
    • Journal of the Korean Society of Costume
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    • v.44
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    • pp.37-56
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    • 1999
  • In clothing, a tendency of Bisexuality is in case of wearing clothing of the opposite sex, the mixing of modeling principle and characteristic of man and woman, the exclusion characteristic of sexuality that present sexlessness-the third sex. They-Designer Gabrielle Chanel, YSL, Giorgio Armani-give characteristic of man's fashion to women's fashion. However, the consciousness in design is different. Chanel apply principle of man's suit to woman's wear to express authority and power about gender image, hereby she express women liberation and equality of man and woman based on social ideology through fashion especially, she express women's sexual independency and self assurance to her clothing. YSL express liberal and bold women that pursuit reasonal and liberal life in 1970. As he apply tailoed suit's rigidity to women's wear, he made 'pants look' universal in women's wear through“Dress for success”in modern life. Armani apply successful modern image to women's wear, so he express women's image of confident intellegent as well as feminine image. He focus women's new image as man living liberal and indivisual life excluding social category rather than will of equality of man and women. Gernreich break sexual prejudice and release women freely, furthermore, as he express concern of sexuality pursuit liberty of mankind surpassing sex distinction with women liberty. Gianni Versace pursuit mix and extinguishment of sexual image many-sided, and break fixed idea of sex, and reprovide feminine and masculine image. So his design tend homosexuality because he regard women's glamor and ostensible sexuality as standard of beauty, he apply this to man's wear as well as women's wear. With this, he expresses sensitive and liberal man's that break characteristic of Gender that symbolize power and authority in man's wear through 'Without ties'.

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Sex-Biased Molecular Signature for Overall Survival of Liver Cancer Patients

  • Kim, Sun Young;Song, Hye Kyung;Lee, Suk Kyeong;Kim, Sang Geon;Woo, Hyun Goo;Yang, Jieun;Noh, Hyun-Jin;Kim, You-Sun;Moon, Aree
    • Biomolecules & Therapeutics
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    • v.28 no.6
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    • pp.491-502
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    • 2020
  • Sex/gender disparity has been shown in the incidence and prognosis of many types of diseases, probably due to differences in genes, physiological conditions such as hormones, and lifestyle between the sexes. The mortality and survival rates of many cancers, especially liver cancer, differ between men and women. Due to the pronounced sex/gender disparity, considering sex/gender may be necessary for the diagnosis and treatment of liver cancer. By analyzing research articles through a PubMed literature search, the present review identified 12 genes which showed practical relevance to cancer and sex disparities. Among the 12 sex-specific genes, 7 genes (BAP1, CTNNB1, FOXA1, GSTO1, GSTP1, IL6, and SRPK1) showed sex-biased function in liver cancer. Here we summarized previous findings of cancer molecular signature including our own analysis, and showed that sex-biased molecular signature CTNNB1High, IL6High, RHOAHigh and GLIPR1Low may serve as a female-specific index for prediction and evaluation of OS in liver cancer patients. This review suggests a potential implication of sex-biased molecular signature in liver cancer, providing a useful information on diagnosis and prediction of disease progression based on gender.

Sex Reverse in Teleost Culture II. Sex Reversal of Guppy and Tilapia by $17\beta$-Estradiol and $17\alpha$-Methyltestoserone (경골어류의 성전환에 관한 연구 II. $17\beta$-Estradiol 및 $17\alpha$-Methyltestosterone을 이용한 Guppy 및 Tilapia의 성전환에 관한 연구)

  • 윤종만;박홍양
    • Korean Journal of Animal Reproduction
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    • v.13 no.1
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    • pp.40-48
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    • 1989
  • Guppy fry were treated for the first 40 days of life with 0, 20, 40, 60 & 100$\mu\textrm{g}$ of estradiol per gram of food in order to change the sex of normal males to functional females(genetic male). The present investigation deals with the effects of steroid hormones, such as $\beta$-estradiol and testosterone, on the sex differentiation in guppy and tilapia. The results obtained were summarized as follows. 1. In B (20$\mu\textrm{g}$/g diet) group 17$\beta$-estradiol-treated, 67.8% of male offsprings were produced. 2. In D (60$\mu\textrm{g}$/g diet) group 17$\beta$-treated, 67% of female offsprings were produced. 3. B, D groups of genetic male brooders had significantly different effects (P<0.01) upon sex ratios of their progeny. 4. This strongly indicates that sex direction has been achieved and that the male is the heterogametic sex. 5. The group that produced the highest percentage of male offspring(male percentage of observed number to expected number was 91%) contained only full-sibling male brooders to the sex-reversed female brooders. 6. After 7 months following treatment, the sex-reversed males had ovarian portion in the anterior region and a testicular portion in the posterior region of the same intersexual gonad, respectively. 7. At 7 months after treatment, the ovareis revealed a complete arrest of the ovarian formation, and appearances of spermatogenetic cell cysts among surviving auxocytes. 8. In most of sex-reversed fish, anterior portion of test is was devoid of sperm ducts including the seminal vesicle and vas deferens. 9. The male transferrin showed two strong bands, while the female transferrin showed a single weak band. 10. One of the two bands of male transferrin showed the same mobility with band of female transferrin.

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