• Journal of Veterinary Science
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• v.21 no.2
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• pp.22.1-22.9
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• 2020

Rabid raccoon dogs (Nyctereutes procyonoides koreensis) have been responsible for animal rabies in South Korea since the 1990s. A recombinant rabies vaccine strain, designated as ERAGS, was constructed for use as a bait vaccine. Therefore, new means of differentiating ERAGS from other rabies virus (RABV) strains will be required in biological manufacturing and diagnostic service centers. In this study, we designed two specific primer sets for differentiation between ERAGS and other RABVs based on mutation in the RABV glycoprotein gene. Polymerase chain reaction analysis of the glycoprotein gene revealed two DNA bands of 383 bp and 583 bp in the ERAGS strain but a single DNA band of 383 bp in the field strains. The detection limits of multiplex reverse transcription polymerase chain reaction (RT-PCR) were 80 and 8 FAID₅₀/reaction for the ERAGS and Evelyn-Rokitnicki-Abelseth strains, respectively. No cross-reactions were detected in the non-RABV reference viruses, including canine distemper virus, parvovirus, canine adenovirus type 1 and 2, and parainfluenza virus. The results of multiplex RT-PCR were 100% consistent with those of the fluorescent antibody test. Therefore, one-step multiplex RT-PCR is likely useful for differentiation between RABVs with and those without mutation at position 333 of the RABV glycoprotein gene.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.18 no.7
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• pp.1968-1985
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• 2024

At present, the world is witnessing a rapid change in all the fields of human civilization business interests and goals of all the sectors are changing very fast. Global changes are taking place quickly in all fields - manufacturing, service, agriculture, and external sectors. There are plenty of hurdles in the emerging technologies in agriculture in the modern days. While adopting such technologies as transparency and trust issues among stakeholders, there arises a pressurized necessity on food suppliers because it has to create sustainable systems not only addressing demand-supply disparities but also ensuring food authenticity. Recent studies have attempted to explore the potential of technologies like blockchain and practices for smart and sustainable agriculture. Besides, this well-researched work investigates how a scientific cum technological blockchain architecture addresses supply chain challenges in Precision Agriculture to take up challenges related to transparency traceability, and security. A robust registration phase, efficient authentication mechanisms, and optimized data management strategies are the key components of the proposed architecture. Through secured key exchange mechanisms and encryption techniques, client's identities are verified with inevitable complexity. The confluence of IoT and blockchain technologies that set up modern farms amplify control within supply chain networks. The practical manifestation of the researchers' novel blockchain architecture that has been executed on the Hyperledger network, exposes a clear validation using corroboration of concept. Through exhaustive experimental analyses that encompass, transaction confirmation time and scalability metrics, the proposed architecture not only demonstrates efficiency but also underscores its usability to meet the demands of contemporary Precision Agriculture systems. However, the scholarly paper based upon a comprehensive overview resolves a solution as a fruitful and impactful contribution to blockchain applications in agriculture supply chains.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.17 no.3
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• pp.103-117
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• 2022

The recent corporate economy, such as the COVID 19 pandemic that has spread all over the world since the beginning of 2020, the acceleration of the 4th industrial revolution, and supply chain management risks triggered by the US-China conflict and the Ukraine crisis, is more serious than ever before. CEOs who have started and managed small and medium-sized enterprises (SMEs) are more concerned than ever about the sustainability of their businesses in this reality. Nevertheless, there were few empirical studies on the factors that influence the intention of SME CEOs to discontinue business. In this study, the perception of the business environment of SMEs (intensity of competition in key business areas, difficulty in manpower management), corporate competency (employee competency, company product or service competitiveness, supply chain and consumer relations, digital competency and technical expertise), and CEO's competency(trust between employees and the CEO, management competency and perceived health status of CEO) on CEO's intention to discontinue business was discussed. As a result of the study, the intensity of competition in the main business field, and the difficulty in manpower management had a positive (+) effect on the intention to discontinue the business, and the employee competency, product (service) competitiveness, digital competency of the company, and the CEO's Health status had a negative (-) effect on intention to discontinue business. The relationship between these influences was found in the order of CEO's health status, product competitiveness, employee competency, digital competency, competitive strength in the main business, and difficulty in manpower management. It was analyzed that supply chain and consumer relations, trust between employees and the CEO, and management capabilities did not significantly affect the intention to discontinue business. On the other hand, business confidence has a mediating effect between the intensity of competition in the main business field, the difficulty in manpower management, product or service competitiveness, digital competency, trust between employees and the CEO, and the management capability and intention to discontinue business was tested. This study had academic significance in that it empirically analyzed factors related to intention to discontinue business targeting small and medium-sized business CEOs. In practice, as it has been found that business environment awareness, corporate competency, managerial competency, and business confidence are factors that influence the intention to discontinue business, if an action ideas that can reinforce this part can be found, SMEs can achieve sustainable growth or it may help CEO find an meaningful exit.

• Journal of the HCI Society of Korea
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• v.2 no.1
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• pp.13-24
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• 2007

For many years, human-computer interaction (HCI) practitioners have focused on usability in order to enhance the user experience, and companies have seen it as an area where they can gain advantages over their competitors. However, a focus on usability limits the potential of HCI research because it restricts the concept of user experience to just an implemented functionality of the information technology (IT). Therefore, it is necessary to expand the boundary of user experience research into a holistic dimension. We suggest that one of the most powerful ways to broadly understand user experience with an IT is to investigate the attributes of an IT and users' perceived values and to construct a user experience structure, a hierarchical structure between the attributes of an IT and users' perceived values. This study thus undertakes two research tasks: to develop a specific methodology, which is the visual probing, for constructing a user experience structure with the attributes of an IT and users' perceived values, and then to build a user experience structure practically by conducting a case study to a specific IT: mobile data service.

• The Transactions of the Korea Information Processing Society
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• v.4 no.1
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• pp.215-223
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• 1997

The advantages of service in satellite links are representatively a long distance and large capacity transfer and simultaneous communication. And terrestrial links are of high speed and high quality data transfer Therefore a combined form of characteristics of satellite and terrestrial links is desirable to furnish a good quality service to subscribers. And in satellite links an analysis method and a simulation method of file transfer are conducted to evaluate the efficiency of file transfer. The parameters are used the message length and transmission time, and the number of chain record and transmission time. From the results of evaluation, in satellite links, it is found that when the message length is shorter than 512 bytes, the file transmission time becomes extremely long and when the message length is set at 1024 bytes, the file transmission time abruptly increase from 2048 bytes. Also it is confirmed that when the number of chain record is under 15, the file transmission time becomes extremely long and if the number increase up to 90,the time becomes gradually short and over 90,it becomes constant.Therefore,from the result of file transmission in th case of utilizing satelite links,it is confirmed that the optimum message length in the minmum transmission time is present closely at 1024 bytes.Also it is confirmed that when the satelite transmission sped becoms smaller from 24kbps to2400bps,the optimum message length also becomes smaller from 640 bytes to 256bytes.

• Spatial Information Research
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• v.4 no.2
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• pp.131-146
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• 1996

The purpose of this study is to extract the main locational factors to affect the location of fastfood stores in Kangnam -Gu, Seoul by using Geographic Information Systems. The Franchise system, which came to be employed in full scale since 1990, now enjoying the booming period, especially fast food industry. The procedure of this research has four steps. First, the spatial distribution of fastfood chain stores in Seoul is analyzed by the land use and road map. Second, the spatial variations of fastfood stores in dong districts of Seoul is explained by multiple regression analysis. Third, the main locational factors to affect the location of fast food stores in Kangnam -Gu are extracted by demand and supply sides using GIS technologies. Finally, the potentiallocational zones where are selected by extracted locational factors are compared with the actual distribution of f astf ood stores in Kangnam - Gu. In terms of demand side, the main locational factors include commercial and service facilities, sub¬ways and bus stops which have a lot of pedestrians, and large apartment districts that have high popu¬lation densities. In terms of supply sides, the main locational factors include land use types and land value, accessibility. After fast food chain stores of Kangnam -Gu are overlaid final potentiallocational zones extracted by demand and supply sides of locational factors, it can be identified that over 80 % of fastfood stores are located in the potentiallocational zone. In conclusion, this study shows that spatial analysis functions may potentially be improved using GIS technologies. However there are still difficulties for the locational analysis for service industries to col¬lect appropriate data in terms of computerized base maps as well as consumer behavior and store characteristics itself.

• Korean Journal of Veterinary Service
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• v.41 no.1
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• pp.29-39
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• 2018

In this study, we developed a sensitive and specific reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid visual detection of foot-and-mouth disease virus (FMDV) circulated in Korea. The RT-LAMP was completed in 40 min at $62^{\circ}C$ and the results of the assay were directly detected by naked eye without any detection process. The assay specifically amplified all 7 serotypes of FMDV RNAs but not amplified other viral and cellular nucleic acids. The sensitivity of the RT-LAMP was $10^2$, $10^3$ and $10^3TCID_{50}/mL$ for serotype O, A and Asia 1 FMDV, respectively, which was comparable to conventional reverse transcription polymerase chain reaction (RT-PCR) and relatively lower than that of real time quantitative RT-PCR (qRT-PCR). Clinical evaluation of the RT-LAMP using different serotypes of Korean and foreign FMDV strains showed a 100% (35/35) agreement with the results of the RT-PCR and qRT-PCR. These results indicated that RT-LAMP assay developed in this study could be a valuable diagnostic method for FMDV monitoring and surveillance.

• Culinary science and hospitality research
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• v.19 no.5
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• pp.206-224
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• 2013

The purpose of this study was to examine the formation of customer's repeated use that is real long-term commitment in franchise/chain coffee shops. 457 effective samples were analyzed using PASW 18.0 and AMOS 18.0. The results of the analysis of the relationships between user experience, customer satisfaction, customer commitment and repeated use are as follows. First, it was presented that atmosphere, price fairness and coffee product quality had a positive impact on customer satisfaction. Second, customer satisfaction had a positive impact on customer commitment and repeated use. Finally, there was the mediating effect of continuous commitment between customer satisfaction and repeated use. Based on the results of the above analysis, the managerial implications for increasing repeated use of coffee shops are as follows. First, the strategy for enhancing performance of user experience is to try to improve atmosphere(facilities, ambient/lighting), price fairness, coffee product quality(taste, flavor, temperature). Second, the strategy for forming repeated use is to enhance customer commitment(affective commitment, continuous/normative commitment).

• Journal of Convergence for Information Technology
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• v.11 no.4
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• pp.83-101
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• 2021

This study is to investigate, using AHP, what technologies should be applied first to Korean SMEs in order to respond to the 4th industrial revolution and change to a smart enterprise. To this end, technologies related to the 4th industrial revolution and smart factory are synthesized, and the classification criteria of Dae-Hoon Kim et al. (2019) are applied, but additional opinions of experts are collected and related technologies are converted to artificial intelligence (AI), Big Data, and Cloud Computing. As a base technology, mobile, Internet of Things (IoT), block chain as hyper-connected technology, unmanned transportation (autonomous driving), robot, 3D printing, drone as a convergence technology, smart manufacturing and logistics, smart healthcare, smart transportation and smart finance were classified as smart industrial technologies. As a result of confirming the priorities for technical use by AHP analysis and calculating the total weight, manufacturing companies have a high ranking in mobile, artificial intelligence (AI), big data, and robots, while service companies are in big data and robots, artificial intelligence (AI), and smart healthcare are ranked high, and in all companies, it is in the order of big data, artificial intelligence (AI), robot, and mobile. Through this study, it was clearly identified which technologies should be applied first in order to respond to the 4th industrial revolution and change to a smart company.

• Korean Journal of Veterinary Service
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• v.45 no.1
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• pp.1-11
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• 2022

A novel porcine circovirus 4 (PCV4) was recently identified in Chinese and Korean pig herds. Although several conventional polymerase chain reaction (cPCR) and real-time PCR (qPCR) assays were used for PCV4 detection, more sensitive and reliable qPCR assay is needed that can simultaneously detect PCV4 and internal positive control (IPC) to avoid false-negative results. In the present study, a duplex qPCR (dqPCR) assay was developed using primers/probe sets targeting the PCV4 Cap gene and pig (glyceraldehyde-3-phosphate dehydrogenase) GAPDH gene as an IPC. The developed dqPCR assay was specifically detected PCV4 but not other PCVs and porcine pathogens, indicating that the newly designed primers/probe set is specific to the PCV4 Cap gene. Furthermore, GAPDH was stably amplified by the dqPCR in all tested viral and clinical samples containing pig cellular materials, indicating the high reliability of the dqPCR assay. The limit of detection of the assay 5 copies of the target PCV4 genes, but the sensitivity of the assay was higher than that of the previously described assays. The assay demonstrated high repeatability and reproducibility, with coefficients of intra-assay and inter-assay variation of less than 1.0%. Clinical evaluation using 102 diseased pig samples from 18 pig farms showed that PCV4 circulated in the Korean pig population. The detection rate of PCV4 obtained using the newly developed dqPCR was 26.5% (27/102), which was higher than that obtained using the previously described cPCR and TaqMan probe-based qPCR and similar to that obtained using the previously described SYBR Green-based qPCR. The dqPCR assay with IPC is highly specific, sensitive, and reliable for detecting PCV4 from clinical samples, and it will be useful for etiological diagnosis, epidemiological study, and control of the PCV4 infections.