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경남지역에서 분리한 Salmonella Enteritidis의 항생제 감수성 검사 및 random amplification polymorphic DNA (RAPD)-PCR을 이용한 유전형 분석 (Analysis of antibiotic susceptibility of Salmonella Enteritidis isolated from Gyeongnam province and the bacterial genotyping by using RAPD-PCR)

  • 김은경;김민경;권현애;윤도경;구정헌;박소연;이희근;조명희;하도윤;김철호;황보원;김상현
    • 한국동물위생학회지
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    • 제41권3호
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    • pp.149-155
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    • 2018
  • Salmonella Enteritidis (S. Enteritidis) are found in animals, humans, and environment. In addition, S. Enteritidis draws attention to the public health concerns due to carriage of antibiotic resistance traits. For these reasons, the prevalence and antibiotic resistance patterns of S. Enteritidis are significant issues with regard to public health. To address this issues, a total of 24 strains of S. Enteritidis from 164 samples collected from several slaughterhouses in Gyeong-Nam province in order for antibiotic resistance profiles. Subsequently, we characterized the genotyping by random amplification polymorphic DNA (RAPD)-PCR. As a result, very high level of resistance to protein synthesis inhibition antibiotics and most isolates were susceptible to others. Six random primers were used for RAPD-PCR to reveal genotypes of S. Enteritidis isolates. One of the primer, P1245, generated 147 distinct RAPD-PCR fragments ranging from 400~3000 bp. The number of RAPD-PCR products ranged from 4 to 8 for this primer. The RAPD-PCR fragments could be placed these strains into 3 subgroups and 2 classes by UPGMA cluster analysis. Interestingly, several S. Enteritidis that isolated from different slaughterhouses showed same genotype. These results showed only limited genetic variation among the isolates, those were grouped into a few different patterns of antibiotic resistance.

경북지역 양돈장의 돼지생식기호흡기증후군, 돼지써코바이러스-2 항체가 조사 (Seroprevalence of porcine reproductive and respiratory syndrome (PRRS) and porcine circovirus-2 (PCV-2) in pig farms in Gyeongbuk province)

  • 손준형;신성호;김순태;이성삼;윤문조;조길재
    • 한국동물위생학회지
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    • 제38권3호
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    • pp.163-166
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    • 2015
  • The purpose of this study was to survey seroprevalence of porcine reproductive and respiratory syndrome (PRRS) virus and porcine circovirus-2 (PCV-2) in Gyeongbuk province by enzyme-linked immunosorbent assay (ELISA). A total of 966 samples collected from 21 pig farms were tested. The sero-positive rate of PRRS and PCV-2 were 77.6% (750/966) and 76.4% (738/966), respectively.

혈청분해시간의 경과가 소 혈액화학치 검사에 미치는 영향 (Effects of Time Elapse of Serum Separation on the Examination of Bovine Blood Chemical Values)

  • 김봉식;허인;윤영순;김종형;김원선
    • 한국동물위생학회지
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    • 제15권2호
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    • pp.128-133
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    • 1992
  • To obtain the basic data for blood chemistry, the effects of the time elapse of serum separation on bovine blood chemical values were investigated. The results obtained are summerized as follows : 1. The constant fluctuation tendency and significance was not detected in GOT, GPT, BUN, Creatinine, ALP, CPX, Cholesterol, Ca, Mg and Pi. 2. Glucose showed the decrease tendency according to the time elapse of serum separation. As the values at 12 and 24 hours showed significant lower values than those at 1 and 2 hours (p<0.01) it was thougth that separation time of serum should be focussed for the glucose determination.

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울진군 한우 Neospora caninum 감염 실태 조사 (Investigation of Neospora caninum infection in Korean indigenous Cattle in Uljin)

  • 전령훈;장영술;이은미;최정혜;박노찬
    • 한국동물위생학회지
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    • 제31권3호
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    • pp.363-367
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    • 2008
  • This study was carried out to investigate an epidemiological state of neosporosis in Korean indigenous cattle in Uljin. Bloody samples were collected from 552 female cattle (337 farms), more than 5 years old, in 10 districts of Uljin, Antibody to N caninum were examined by enzyme linked immunosorbent assay (ELISA). Seroprevalence of individual and farm were 7.6% (42/552) and 8.6% (29/337), respectively. Positive rates by districts was variable $(0%{\sim}14,9%)$, and it was seemed to be a tendency that positive farms gathered with in a small zone. In seroprevalence by herd size farms having between 5-9 heads was top (37%), but there was no significant difference among herd size.

재래산양의 설사병 병원체 감염률 조사 (Status of diarrhea pathogens from Korean indigenous goat feces)

  • 손준형;이재봉;황유선;김상윤;김석환
    • 한국동물위생학회지
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    • 제40권1호
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    • pp.21-25
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    • 2017
  • The purpose of this study was to survey on infection status of pathogens of diarrhea from Korean indigenous goat. A total of 800 fecal samples was collected from 50 farms from January to November 2016 and was tested by automatic biochemical machine and polymerase chain reaction (PCR). The overall infection rates of parasitic, bacterial and viral pathogens was 13.0%, 23.0%, 11.3% and the rates of coccidia, Escherichia coli (E. coli), bovine viral diarrhea virus (BVDV), rotavirus and coronavirus were 13.0%, 23.0%, 5.3%, 8.8% and 2.6%, respectively. In the rates of mixed detection, single was 29.8%, double 5.1%, triple 2.8%, quadruple 1.1% in each sample, respectively.

밀폐공간에서의 화재 위험성에 관한 연구 (A Study on fire risk in an enclosure room)

  • 김수영;김홍식;한동훈;이성룡;김주석;육근환;천성수
    • 한국화재소방학회:학술대회논문집
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    • 한국화재소방학회 2009년도 춘계학술논문발표회 논문집
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    • pp.449-453
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    • 2009
  • 기존 주택 및 고층건물화재에서 발생하는 화재 폭발 사고에 대해서는 많은 연구가 이루어지고 있으나, 이천 냉동창고 화재와 같이 많은 인명피해가 발생되는 작업환경에서의 화재 특히 밀폐공간에서의 화재 폭발 위험성 및 특성은 주택 및 고층건물화재에 비교하여 높은 폭발위험성 및 빠른 화염전파 특성을 보이나 이에 관한여 구체적인 연구는 미비한 실정이다. 본 연구는 밀폐공간 작업환경에서의 화재 폭발 위험성 특성 분석을 위하여 밀폐공간에서 작업 시 발생되어지는 가연성 가스의 확산 특성 분석 및 폭발 위험성 분석 방법에 관한 연구와 밀폐공간에서의 화염 확산 특성에 관한 연구논문 이다.

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Serologlcal survey of infections agents in domesticated boars

  • Cho, Kwang-Hyun;Park, In-Hwa;Kim, Young-Hoan;Kim, Soon-Tae;Kim, Sung-Kook;Park, No-Chan;Son, Jae-Kwon;Jyeong, Jong-Sik
    • 한국동물위생학회지
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    • 제24권4호
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    • pp.359-367
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    • 2001
  • A serological survey was performed to establish basic data for the prevalence of antibodies to some major diseases of domesticated boar serum samples from January to December 2000. Sera collected in breeding farms in Gyeongbuk province were tested for Aujeszky's disease virus(ADV), Porcine reproductive and respiratory syndrome virus(PRRSV), Porcine parvovirus(PPV), Japanese encephalitis virus (JEV), Bordetella bronchiseptica(B bronchiseptica), Mycoplasma ; APP), Toxoplasma, and Brucella. There was no antibody to ADV in domesticated boars serum samples detected by Anti-ADV-gpI assay kit. Sero-positive samples to PRRS by IFA were 0.9%(3/330) The HI titers to PPV ranged variously from less than 10 to over 1,280. Two hundred ninety-four out of 330 tested sera showed HI titer of less than 10. In HI test to JEV, 90.3% of the sera (298/330) were below 10. The majority of the serum samples had low prevalence of the antibody B bronchiseptica. ELISA titers to M hyopneumoniae ranged variously from $\leq$ 10 to $\geq$ 1,280. Antibody titers to A pleuropneumoniae type 2(APP2) and type 5(APP5) were 58.2% and 52.7%, respectively, and the tested samples showing ELISA antibody titers of less than 20. There was no significant geographical difference between APP2 and APP5 in this study. In the antibody test of Toxoplasma, 11.5%(38/330) were positive and samples were all negative in sera test of Brucella.

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강원도내 광견병 발생실태 (Report on The Incidence of rabies in Kangwon-do)

  • 김종술;정동수;임환;양윤모;박양순;신명균;김동균
    • 한국동물위생학회지
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    • 제18권1호
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    • pp.57-71
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    • 1995
  • During the period of 2 years from 1993 to 1994, epidemiological investigation were carried out each time the outbreak of rabies suspected cases were informed and all specimens were transported to the Kangwon-do Veterinary Service Lab. At the Lab., autopsy and laboratory examinations were conducted as well. The results obtained as follows 1. The dogs and cattles were confirmed to be infected with rabies virus by routine histopathological findings, indirect flulrescent antibody technique, and mouth inoculation test. 2. The total numbers of rabies occured in dogs and cattles were 9, 5 heads, respectively. The regional distribution of total of 14 heads were 9 heads in Chulwon, 2 heads in Hwachun, 1 head Yangku, 2 heads in Inje and the highest number of outbreaks were orrured in Chulwon. 3. Most of dogs showed furious type and dumb type found in only one dog. 4. Cattles showed the symtoms of uneasiness feeling, excitement, disturbance howling, the change of voice, sexual accelaration, foreign body swatting and attacked to human and ani-mal and found to be died after two or four days. 5. In the seasonal outbreaks of rabies, 8 heads of the highest incidence were occured in win-ter followed by 4 heads in autumn, 1 head in spring and 1 head in summer. 6. The first outbreaks of rabies was reported in Chulwon and then transmitted into Hwachun, Yangku and Inje in turn. 7 The infectious source was supposed to be wild animals such as laccon dog, badger etc. living around the D. M. Z.

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