Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Analysis of antibiotic susceptibility of Salmonella Enteritidis isolated from Gyeongnam province and the bacterial genotyping by using RAPD-PCR

경남지역에서 분리한 Salmonella Enteritidis의 항생제 감수성 검사 및 random amplification polymorphic DNA (RAPD)-PCR을 이용한 유전형 분석

  • 김은경 (경상남도 동물위생시험소) ;
  • 김민경 (경상남도 동물위생시험소) ;
  • 권현애 (경상남도 동물위생시험소) ;
  • 윤도경 (경상남도 동물위생시험소) ;
  • 구정헌 (경상남도 동물위생시험소) ;
  • 박소연 (경상남도 동물위생시험소) ;
  • 이희근 (경상남도 동물위생시험소) ;
  • 조명희 (경상남도 동물위생시험소) ;
  • 하도윤 (경상남도 동물위생시험소) ;
  • 김철호 (경상남도 동물위생시험소) ;
  • 황보원 (경상남도 동물위생시험소) ;
  • 김상현 (경상대학교 수의과대학 수의미생물학연구실)
  • Received : 2018.05.08
  • Accepted : 2018.09.21
  • Published : 2018.09.30
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Abstract

Salmonella Enteritidis (S. Enteritidis) are found in animals, humans, and environment. In addition, S. Enteritidis draws attention to the public health concerns due to carriage of antibiotic resistance traits. For these reasons, the prevalence and antibiotic resistance patterns of S. Enteritidis are significant issues with regard to public health. To address this issues, a total of 24 strains of S. Enteritidis from 164 samples collected from several slaughterhouses in Gyeong-Nam province in order for antibiotic resistance profiles. Subsequently, we characterized the genotyping by random amplification polymorphic DNA (RAPD)-PCR. As a result, very high level of resistance to protein synthesis inhibition antibiotics and most isolates were susceptible to others. Six random primers were used for RAPD-PCR to reveal genotypes of S. Enteritidis isolates. One of the primer, P1245, generated 147 distinct RAPD-PCR fragments ranging from 400~3000 bp. The number of RAPD-PCR products ranged from 4 to 8 for this primer. The RAPD-PCR fragments could be placed these strains into 3 subgroups and 2 classes by UPGMA cluster analysis. Interestingly, several S. Enteritidis that isolated from different slaughterhouses showed same genotype. These results showed only limited genetic variation among the isolates, those were grouped into a few different patterns of antibiotic resistance.

Keywords

References

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