• The Journal of Korean Institute of Communications and Information Sciences
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• v.22 no.10
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• pp.2357-2363
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• 1997

In this paepr, we show that the length of the test sequence can be reduced using non-minimal multiple UIO instead of minimal multiple UIO. Our test sequence generation method starts from the observation that Shen's approach cannot optimize the length of the test sequence whtn the difference between the number of incoming edge and that of outgoing edge is large. Usig the property, we propse a more efficient method to generate a UIO-based test sequence in terms of the length, and compares our method with other existing methods in terms of execution time for comformancetesting and time for generation of test sequence.

• The Plant Pathology Journal
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• v.15 no.6
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• pp.313-318
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• 1999

Cloning of the 5'untranslated region (5' UTR) and Nterminus of the glycoprotein precursor (G2G1) open reading frame of tomato spotted wilt virus has been problematic, possibly because of the toxicity of a signal peptide at the beginning of th G2G1 protein precursor. The toxicity of the signal peptide to bacterial growth and the reason for the expression of the peptide gene in Escherichia coli were investigated by cloning the 5' UTR and the signal peptide sequence separately. Cells transformed with the plasmid containing both the first 30 amino acids of the glycoprotein and the 5' UTR showed a severe growth inhibition whereas transformants harboring either the plasmid with the signal sequence or the 5'UTR alone did not show any ingibition. An E. coli promoter-like sequence was found in the 5'UTR and tis promoter acivity was confirmed with a promoter-less GUS gene cloned downstream of the 5'UTR. In the cloning of the Tomato spotted wilt virus (TSWV) glycoprotein G2G1 open reading frame all the recovered plasmids contained stop codons in the signal sequence region. However, clones containing no stop codon were recovered when the signal sequence and the 5'UTR were cloned separately.

• Genomics & Informatics
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• v.6 no.2
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• pp.72-76
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• 2008

Hypoxia-inducible factor $1{\alpha}\;(HIF1{\alpha})$ is a transcription factor that plays a key role in the adaptation of cells to low oxygen stress and oxygen homeostasis. The oxygen-dependent degradation (ODD) domain of $HIF1{\alpha}$ is responsible for the negative regulation of $HIF1{\alpha}$ in normoxia. The interactions of the $HIF1{\alpha}$ ODD domain with partner proteins such as von Hippel-Lindau tumor suppressor (pVHL) and p53 are mediated by two sequence motifs, the N- and C-terminal ODD(NODD and CODD). Multiple sequence alignment with $HIF1{\alpha}$ homologs from human, monkey, pig, rat, mouse, chicken, frog, and zebrafish has demonstrated that the NODD and CODD motifs have noticeably high conservation of the primary sequence across different species and isoforms. In this study, we carried out molecular dynamics simulation of the structure of the $HIF1{\alpha}$ CODD motif in complex with the p53 DNA-binding domain (DBD). The structure reveals specific functional roles of highly conserved residues in the CODD sequence motif of $HIF1{\alpha}$ for the recognition of p53.

• The Transactions of the Korean Institute of Electrical Engineers A
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• v.52 no.3
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• pp.183-192
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• 2003

This paper presents the real-time implemented distance relay algorithm which the fault distance is estimated with only local terminal information. When a single-phase-to-earth fault on a two-parallel transmission line occurs, the reach accuracy of distance relay is considerably affected by the unknown variables which are fault resistance, fault current at the fault point and zero- sequence current of sound line The zero-sequence current of sound line is estimated by using the zero sequence voltage which is measured by relaying location Also. the fault resistance is removed at the Process of numerical formula expansion. Lastly, the fault current through a fault point is expressed as a function of the zero-sequence current of fault line, zero-sequence current of sound line, and line, and fault distance. Therefore, the fault phase voltage can be expressed as the quadratic equation of the fault distance. The solution of this Quadratic equation is obtained by using a coefficient of the modified quadratic equation instead of using the square root solution method. After tile accurate fault distance is estimated. the mote accurate impedance is measured by using such an information.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.34 no.1C
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• pp.113-119
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• 2009

The training sequence with the appropriate length for equalization and initial synchronization is necessary before sending the pure data in the burst transmission type DS-UWB system. The length of the training sequence is one of the factors which make throughput decreased. The noble structure with the variable length of the training sequence whose length can be adaptively tailored according to the channel conditions (CM1,CM2,CM3,CM4) in the DS-USB systems is proposed. This structure can increase the throughput without sacrificing the performance than the method with fixed length of training sequence considering the worst case channel conditions. Simulation results under IEEE 802.15.3a channel model show that the proposed scheme can achieve higher throughput than a conventional one with the slight loss of BER performance. And this structure can reduce the computation complexity and power consumption with selecting the short length of the training sequence.

• The KIPS Transactions:PartB
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• v.15B no.3
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• pp.179-188
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• 2008

In this paper, knowledge based text to facial sequence image system for interaction of lecturer and learner in cyber universities is studied. The system is defined by the synthesis of facial sequence image which is synchronized the lip according to the text information based on grammatical characteristic of hangul. For the implementation of the system, the transformation method that the text information is transformed into the phoneme code, the deformation rules of mouse shape which can be changed according to the code of phonemes, and the synthesis method of facial sequence image by using deformation rules of mouse shape are proposed. In the proposed method, all syllables of hangul are represented 10 principal mouse shape and 78 compound mouse shape according to the pronunciation characteristics of the basic consonants and vowels, and the characteristics of the articulation rules, respectively. To synthesize the real time facial sequence image able to realize the PC, the 88 mouth shape stored data base are used without the synthesis of mouse shape in each frame. To verify the validity of the proposed method the various synthesis of facial sequence image transformed from the text information is accomplished, and the system that can be applied the PC is implemented using the proposed method.

• Animal cells and systems
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• v.6 no.3
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• pp.271-275
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• 2002

Rph1 and Gisl are damage-responsive repressors involved in PHR1 expression. They have two $C_{2}$H/ sub 2/ zinc finger motifs as putative DNA binding domains and N-terminal conserved domain with unknown function. They are also found in the human retinoblastoma binding protein 2 and the mouse jumonji- encoded protein. The repressors are able to bind to A $G_{4}$ sequence within a 39-bp sequence called upstream repressing sequence of PHR1 promoter (UR $S_{PHR1}$) responsible for the damage-response of PHR1. We report here that Rph1 is predominantly localized in the nucleus as examined by fluorescence microscopic analysis with GFP-Rph1 fusion protein. On the basis of the fact that the A $G_{4}$ sequence that is recognized by Rph1 and Gisl is also recognized by Msn2 and Msn4 in a process of stress response, we a1so tried to examine the in vivo function of A $G_{4}$ and the role of Msn2 and Msn4 in PHR1 expression. Our results demonstrate that Msn2 and Msn4 are actually required for the basal transcription of PHR1 expression but not for its damage induction. When A $G_{4}$ sequence was inserted into the minimal promoter of the cyc1-LacZ reporter, the increased LacZ expression was observed indicating its involvement in transcriptional activation. The data suggest that the A $G_{4}$ is primarily required for basal transcriptional activation of PHR1 or CYC1 promoter through the possible involvement of Msn2 and Msn4. However, since the A $G_{4}$ is also involved in the repression of PHR1 via Rphl and Gisl, it is proposed that A $G_{4}$ functions as either URS or upstream activating sequence (UAS) depending on the promoter context.t.

• Korean Journal of Plant Tissue Culture
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• v.24 no.2
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• pp.113-117
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• 1997

Simple sequence repeats (SSR) are widely dispersed throughout eukaryotic genomes, highly polymorphic, and easily typed using polymerase chain reaction (PCR). The objective of this study was to determine the polymorphism of different Chlamydomonas reinhartdtii strains and to determine the mode of inheritance of the SSR locus in Chlamydomonas. A genomic DNA library of C. reinhardtii was constructed and screened with a radiolabeled $(AC)_{11}$ probe for the selection of (CA/GT)n repeat clone. Selected clone was seqeuenced, and PCR primer set flanking (CA/GT)n sequence was constructed. PCR was used to specifically amplify the SSR locus from multiple isolates of C. reinhardtii. The locus was polymorphic in some of the C. reinhardtii isolates. However, the locus was amplified only 4 of 6 isolates of C. reinhardtii, not in other 2 isolates of C. reinhardtii, suggesting that this locus is not extensively conserved. A simple Mendelian inheritance pattern was found, which showed 2:2 segregation in the tetrads resulting from a cross between C. reinhardtii and C. smithii. Our results suggest that this simple sequence repeat DNA polymorphism will be useful for identity testing, population studies, linkage analysis, and genome mapping in Chlamydomonas.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.8 no.4
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• pp.263-269
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• 2015

Digital watermarking is imperceptible and statistically undetectable information embeds into digital data. Most information in digital audio watermarking schemes have used binary random sequences. The embedded binary random sequence distorts and modifies the original data while it plays a vital role in security. In this paper, a binary random sequence to improve imperceptibility in perceptual region of the human auditory system is proposed. The basic idea of this work is a modification of a binary random sequence according to the frequency analysis of adjacent binary digits that have different signs in the sequence. The canonical signed digit code (CSDC) is also applied to modify a general binary random sequence and the pair-matching function between original and its modified version. In our experiment, frequency characteristics of the proposed binary random sequence was evaluated and analyzed by Bark scale representation of frequency and frequency gains.

• Physical Therapy Rehabilitation Science
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• v.8 no.4
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• pp.225-233
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• 2019

Objective: The purpose of this study was to examine the possible effects of incorporating short-term slow-abdominal respiration (SAR) into an exercise program, on balance and the cardiac-related autonomic nervous system (ANS). Design: Cross-over repeated measures design. Methods: Fifteen young and healthy adults were randomly assigned into two groups (7 in the C-R group, 8 in the R-C group), each of which carried out both control sequence (C) and respiration-experiment sequence (R) in the inverse order. In the C sequence, the subjects performed passive exercises and a general exercise program (P-GEP). In the R sequence, the subjects received a short-term SAR training session and then performed the respiration incorporated general exercises program (R-RGEP). Before and after both C and R sequences, the length and the area of the displacement of the center of pressure (COP) and heart rate variability parameters were measured. Results: The total length of the COP displacement in the left single-leg-standing condition showed a significantly greater reduction after R-RGEP in the respiration-experiment sequence than after the P-GEP in the control sequence (p<0.05). The mean heart rate was significantly reduced only after R-RGEP in the respiration-experiment sequence (p<0.05) Conclusions: The slow-abdominal-respiration, trained in a simple manner and integrated into the exercise program in a single session, showed partially positive immediate effects on balance stabilization. The decrease in heart rate indicated possible involvement of the parasympathetic ANS activation in the stability, although it is not enough to decide whether it is purely due to the controlled respiration.