• Title/Summary/Keyword: Sequence to Sequence

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Sequencing of the RSDA Gene Encoding Raw Starch-Digesting $\alpha$-Amylase of Bacillus circulans F-2: Identification of Possible Two Domains for Raw Substrate-Adsorption and Substrate-Hydrolysis

  • Kim, Cheorl-Ho
    • Journal of Microbiology and Biotechnology
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    • v.2 no.1
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    • pp.56-65
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    • 1992
  • The complete nucleotide sequence of the Bacillus circulans F-2 RSDA gene, coding for raw starch digesting a-amylase (RSDA), has been determined. The RSDA structure gene consists of an open reading frame of 2508 bp. Six bp upstream of the translational start codon of the RSDA is a typical gram-positive Shine-Dalgarno sequence and the RSDA encodes a preprotein of 836 amino acids with an Mr of 96, 727. The gene was expressed from its own regulatory region in E. coli and two putative consensus promoter sequences were identified upstream of a ribosome binding site and an ATG start codon. Confirmation of the nucleotide sequence was obtained and the signal peptide cleavage site was identified by comparing the predicted amino acid sequence with that derived by N-terminal analysis of the purified RSDA. The deduced N-terminal region of the RSDA conforms to the general pattern for the signal peptides of secreted prokaryotic proteins. The complete amino acid sequence was deduced and homology with other enzymes was compared. The results suggested that the Thr-Ser-rich hinge region and the non-catalytic domain are necessary for efficient adsorption onto raw substrates, and the catalytic domain (60 kDa) is necessary for the hydrolysis of substrates, as suggested in previous studies (8, 9).

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Analysis of Kronecker Sequence with Partial-Period Correlation in a Multiple-dwell Serical Serarch System (복수적분 시구간 직렬탐색 시스템에서 부분 상관기를 이용한 Kronecker 부호의 특성 분석)

  • 임연주;박상규
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.25 no.8B
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    • pp.1333-1340
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    • 2000
  • This paper shows that the Kronecker sequence, the rapid acquisition spreading code, can be used in packet wireless data communication systems. The general properties of the Kronecker sequence such as construction and correlation characteristics are described, and it is shown that the Kronecker sequence can use a partial-period correlation for a faster acquisition. Based on above Properties, it can be expected that the Kronecker sequence can be used in packet communication systems because the probability of false alarm for the Kronecker sequence is lower and flatter(that is, less sensitive to Ec/No variations) than that for the PN sequence under the assumption that both sequences have the same acquisition time.

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Performance Analysis of Frequency Synchronization for HDR-WPAN System (HDR-WPAN 시스템을 위한 주파수 동기 성능분석)

  • Park, Ji-Woo;Kang, Hee-Gok;Kim, Jae-Young;Oh, Chang-Heon
    • Journal of Advanced Navigation Technology
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    • v.8 no.2
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    • pp.163-168
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    • 2004
  • In this paper, we propose a frequency synchronization algorithm using characteristic of CAZAC sequence for HDR-WPAN and analyze the performance by signal constellation and EVM(error vector magnitude). The proposed frequency offset technique estimated each sample phase error of two sequences among 12 CAZAC sequences which have excellent autocorrelated characteristic. Estimated phase error is multiplied to each sample of next sequence for compensating the frequency offset. The remaining frequency offset after compensating it with two sequences has maximum 0.002 offsest ranges at each sample. The computer simulation proved that the permission of EVM value had satisfied in the case of DQPSK at 20[dB].

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THE G-SEQUENCE OF A MAP AND ITS EXACTNESS

  • Pan, Ian-Zhong;Shen, Xin-Yao;Woo, Moo-Ha
    • Journal of the Korean Mathematical Society
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    • v.35 no.2
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    • pp.281-294
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    • 1998
  • In this paper, we extend the G-sequence of a CW-pair to the G-sequence of a map and show the existence of a map with nonexact G-sequence. We also give an example of a finite CW-pair with nontrivial $\omega$-homology in high order.

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ON SEQUENCE OF FUNCTIONS

  • Salehbhai, Ibrahim A.;Prajapati, Jyotindra C.;Shukla, Ajay K.
    • Communications of the Korean Mathematical Society
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    • v.28 no.1
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    • pp.123-134
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    • 2013
  • Operational techniques have drawn the attention of several researchers in the study of sequence of functions and polynomials. An attempt is made to introduce a new sequence of functions by using operational techniques. Some generating relations and finite summation formulae have been obtained. The corresponding MAPLE code for obtaining above sequence of functions for different values of parameters was also discussed.

SUM FORMULAE OF GENERALIZED FIBONACCI AND LUCAS NUMBERS

  • Cerin, Zvonko;Bitim, Bahar Demirturk;Keskin, Refik
    • Honam Mathematical Journal
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    • v.40 no.1
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    • pp.199-210
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    • 2018
  • In this paper we obtain some formulae for several sums of generalized Fibonacci numbers $U_n$ and generalized Lucas numbers $V_n$ and their dual forms $G_n$ and $H_n$ by using extensions of an interesting identity by A. R. Amini for Fibonacci numbers to these four kinds of generalizations and their first and second derivatives.

Automatic generation of sequence control programs

  • Gohi, Tetuji;Kojima, Fumio;Obana, Hideo;Sugimori, Hisayosi;Tsukimoto, Hirosi
    • 제어로봇시스템학회:학술대회논문집
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    • 1992.10b
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    • pp.463-467
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    • 1992
  • This paper describes the automatic generation of sequence control programs for DCS(Distributed Control System), PLC(Programable Logic Controller) and so on. Since there is no same manufacturing process, it is difficult to standardize sequence programs. We propose the automatic sequence control program generator which is CAD software using knowledge engineering technique.

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MAYER-VIETORIS SEQUENCE IN COHOMOLOGY OF LIE ALGEBROIDS ON SIMPLICIAL COMPLEXES

  • Oliveira, Jose R.
    • Communications of the Korean Mathematical Society
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    • v.33 no.4
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    • pp.1357-1366
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    • 2018
  • It is shown that the Mayer-Vietoris sequence holds for the cohomology of complexes of Lie algebroids which are defined on simplicial complexes and satisfy the compatibility condition concerning restrictions to the faces of each simplex. The Mayer-Vietoris sequence will be obtained as a consequence of the extension lemma for piecewise smooth forms defined on complexes of Lie algebroids.

Nucleotide Sequence Analysis of the RNA-dependent RNA Polymerase Gene of Infectious Pancreatic Necrosis Virus DRT Strain

  • Lee, Hyung-Hoan;Chung, Hye-Kyung;Lee, Seong-Hun
    • Journal of Microbiology and Biotechnology
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    • v.4 no.4
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    • pp.264-269
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    • 1994
  • To determine the nucleotide sequence of the ds RNA segment B containing the RNA dependent RNA polymerase (RdRp) gene of the DRT strain of infectious pancreatic necrosis virus (lPNV), the cDNA of the ds RNA segment B of the DRT strain of IPNV was synthesized using the reverse transcriptase (RT)-polymerase chain reaction (PCR) and its cDNA nucleotide sequence was determined. The DRT segment B was 2, 783 bp long and contained only a single long open reading frame (ORF) of 2, 535 bp in length. This ORF nucleotides encoded the VPl protein, the putative RdRp of IPNV. The VPl protein comsisted of 845 amino acids. The molecular weight of the RdRp, as deduced from the nucleotide sequence, is 94, 426. The nucleotide sequence of the ORF of the DRT showed 89.7% homology to the Jasper strain, but 80.8% to the Sp strain. The amino acid sequence of the ORF of the DRT sho.wed 97.6% homology to the Jasper strain, but 88.7% to the Sp strain. The conserved GTP-binding motif was detected in VPl protein.

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Identification of Novel Cupredoxin Homologs Using Overlapped Conserved Residues Based Approach

  • Goyal, Amit;Madan, Bharat;Hwang, Kyu-Suk;Lee, Sun-Gu
    • Journal of Microbiology and Biotechnology
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    • v.25 no.1
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    • pp.127-136
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    • 2015
  • Cupredoxin-like proteins are mainly copper-binding proteins that conserve a typical rigid Greek-key arrangement consisting of an eight-stranded β-sandwich, even though they share as little as 10-15% sequence similarity. The electron transport function of the Cupredoxins is critical for respiration and photosynthesis, and the proteins have therapeutic potential. Despite their crucial biological functions, the identification of the distant Cupredoxin homologs has been a difficult task due to their low sequence identity. In this study, the overlapped conserved residue (OCR) fingerprint for the Cupredoxin superfamily, which consists of conserved residues in three aspects (i.e., the sequence, structure, and intramolecular interaction), was used to detect the novel Cupredoxin homologs in the NCBI non-redundant protein sequence database. The OCR fingerprint could identify 54 potential Cupredoxin sequences, which were validated by scanning them against the conserved Cupredoxin motif near the Cu-binding site. This study also attempted to model the 3D structures and to predict the functions of the identified potential Cupredoxins. This study suggests that the OCR-based approach can be used efficiently to detect novel homologous proteins with low sequence identity, such as Cupredoxins.