• Bulletin of the Korean Chemical Society
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• 제21권11호
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• pp.1077-1084
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• 2000

The dipolar effect of neighboring protons that are not directly bonded to the carbon of interest on coupled carbon-13 relaxation in a simple organic molecule has been studied by comparing the relaxation behaviors of labeled carbon-13 in $Br13CH_2COOH$ with those in $BrCH_213COOH.$ Various pulse sequences, such as coupled inversion recovery pulse sequence, J-negative and J-positive pulse sequence, and nonselective and selective proton ${\pi}pulse$ sequence, were employed to perform the required coupled spin relaxation experiments. To gain information on various spectral densities, including that of dipolar-CSA cross correlation, the experiments were performed on two different spectrometers, operating, respectively, at 50.31 and 125.51MHz for 13C. The magnitude of CH dipolar spectral densities for $BrCH_213COOH$ was found to be about 8% of those for $Br13CH_2COOH$, which means the effect due to the protons not directly bonded to the carbon of interest is small but not completely negligible.

• Asian-Australasian Journal of Animal Sciences
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• 제20권6호
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• pp.850-855
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• 2007

Leptin is the adipocyte-specific product of the obese gene and plays a major role in food intake and energy metabolism. Leptin research was mainly focused on mammalian species, but understanding of leptin and its function in poultry is very poor. In this study, the duck leptin gene was amplified using the reverse transcription-polymerase chain reaction (RT-PCR) from duck liver RNA. The cDNA fragment was inserted into the pET-28a expression vector, and the resulting plasmid was expressed in Escherichia coli BL21 (DE3). Experimental mice were given an intraperitoneal injection of 10 mg/kg leptin dissolved in phosphate buffered saline (PBS), while the control mice were injected with PBS. The effect of leptin on food intake, body weight and fatty deposition in mice was detected. Sequence analysis revealed that duck leptin had a length of 438 nucleotides which encoded a peptide with 146 amino acid residues. The sequence shares highly homology to other animals. The coding sequence of duck leptin was 84 and 86% identical to human and pig leptin nucleotides sequence. Highest identity was with the rat coding sequence (95%). The identity of the amino acid sequence was 84, 82 and 96% respectively compared to that of the human, pig and rat. Results of SDS-PAGE analysis indicated that a fusion protein was specifically expressed in E. coli BL21 (DE3). The purified product was found to be biologically active during tests. Continuous administration of recombinant duck leptin inhibited food intake. Despite the decrease of food intake, leptin significantly induced body weight and fatty deposition. These changes were accompanied by a significant down-secretion of plasma glucose, cholesterol, triglyceride and insulin levels in mice. The observations provide evidence for an inhibitory effect of leptin in the regulation of food intake and for a potential role of duck leptin in the regulation of lipogenesis.

• 한국미생물·생명공학회지
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• 제27권6호
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• pp.440-445
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• 1999

In order to investigate structure-antibiotic activity relationships of brevinin-1 and thanatin containing Rana box composed of basic loop formed by disulfide bridge in their arboxy terminus, thanatin, brevinin 1 and their analogues (T-B1, T-B2 and B-T) in which their Rana box sequence exchanged was designed and synthesized by the solid phase method using Fmoc-chemistry. The basic sequence of Rana box of thanatin had more significant effect on both antibacterial and antifungal activity than that of brevinin 1. The tail sequence (QRM) of thanatin was found to be important in its antibacterial and antifungal activity. Rana box sequence of brevinin-1 did not have a significant effect on its antitumor and phospholipid vesicle-aggregating activities. Brevinin-1 showed stronger $\alpha$-helical structure in the membrane-mimicking environment such as SDS micelle than thanatin. A remarkable increase in a-helicity of bervinin-1 plays more important role in antibiotic activity than that of thanatin. Furthermore, antibacterial activity of thanatin against E. coli resulted from the disruptive effect against the outer cell membrane of E. coli.

• Journal of Magnetics
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• 제21권1호
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• pp.148-152
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• 2016

The main goal was to evaluate effectiveness of a modified TSE sequence compared with DIR (double inversion recovery) sequence in acquisition of fast flow brain vessel images using signal void effect. 32 healthy volunteers (10 men and 22 women; mean age of 31 years; ranging between 28-43 years) who underwent black blood DIR sequence (group A) and the modified TSE sequence (group B) were enrolled in our study. Signal to Noise Ratio (SNR) and Contrast to Noise Ratio (CNR) of the internal carotid arteries' lumen were compared in T1 and T2 weighted images for both group A and B. The images obtained from group B showed lower SNR values in internal carotid artery than the group A in both of the T1 and T2 weighted images (11.49% and 13.66% respectively). While the CNR values were higher in the group B than the group A in both of the T1 and T2 weighted images (8.69% and 7.55 % respectively). The qualitative score of all categories were not significantly different between the two groups. Furthermore approximately 49% of the total scan time was reduced from group B. Our study is to shorten the scanning time and minimize the inconveniences of the patients in acquisition of the black blood images of brain by using the signal void effect in the modified TSE technique while keeping the diagnostic value of the test.

• 한국인쇄학회지
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• 제16권1호
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• pp.13-24
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• 1998

The color difference caused by the overprinting sequence of ink in multicolor printing is due to both trapping and optical properties of ink layers. Hence the effect of optical properties only cannot be analyzed without removing the effect of ink trapping This study was carrier out for the purpose of analyzing optically the color difference caused by only the optical properties of ink under the various printing sequence. The present optical analysis for overprints showed a good agreement with the experimental result. It is expected that this study may contribute to decreasing the color difference between the original and the printed reproduction in multi-color printing.

• Communications for Statistical Applications and Methods
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• 제6권2호
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• pp.601-610
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• 1999

Crossover design is officially except for special occasions recommended by KFDA and FDA for assessing Bioequivalence between two drugs one for reference and the other for innovator. Such design is regarded as a special case of latin square split-polt or repeated measurement design and its main difference with other designing methods is that each subject in an experiment is exposed two drugs in sequence. Therefore general statistical analysis is not suitable since the model for this experiment includes carryover effect in addition to period and sequence effect. in this paper analysis for crossover model with two drugs and its interpretation are mainly discussed and an example is given for illustration.

• 한국안전학회지
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• 제16권4호
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• pp.123-127
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• 2001

Numerous PSC bridges are stregthened by the combined use of continuity of simple spans and addition of external prestressing. In this case prestressing sequences should be carefully checked due to the effect on the stress and camber of girders and slab. Various prestressing sequences were applied in this field test and measured values were analysed. This results show that preatressing sequences affact the stress and deflection of bridge members, so the prestressing sequence should be considered at the desist and construction stages of deteriorated bridges.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 2005년도 춘계 학술발표회 논문집
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• pp.601-606
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• 2005

Welding deformations are affected by various factors. This research investigates effects of welding sequence and self-weight on welding deformation. According to the results by equivalence load method, magnitude of welding deformation with self-weight is about twice one without self-weight on parallel weld path component. But welding deformation with the components used in this research are not affected by welding sequence

• Journal of Electrical Engineering and information Science
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• 제2권4호
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• pp.19-22
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• 1997

In this paper, we propose a new communciation scheme combining both MT modulation and DS-CDMA with a concatenated orthogonal/PN spreading sequence. This scheme incorporates the advantages of both DS-CDMA wit a concatenated sequence, and an MT modulation technique to combat the effect of a multipath fading channel. It is shown that he proposed system outperforms the MT-CDMA system with a convetional PN sequence.

• 대한안전경영과학회지
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• 제3권4호
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• pp.103-112
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• 2001

In Factory, as the number of machine is increased the more maintenance efforts are necessary. Multi maintenance issues may occur at a certain time and the determination of maintenance sequence is needed. In this study, we first compare the priority of machines and the impact value using modified FMEA(Failure Mode Effect and Analysis) method. Also, CBR(Case-based Reasoning) approach is applied to retrieve similar fault cases of current machine problem. The proposed methodology will be useful to implement decision support system of maintenance sequence for CMMS/EAM (Computerized Maintenance Management System/Enterprise Asset Management).