• Biotechnology and Bioprocess Engineering:BBE
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• v.8 no.4
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• pp.227-232
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• 2003

A self-assembled monolayer of protein G was fabricated to develop an immunosensor based on surface plasmon resonance (SPR), thereby improving the performance of the antibodybased biosensor through immobilizing the antibody molecules (lgG). As such, 11-mercaptoundecanoic acid (11-MUA) was adsorbed on a gold (Au) support, while the non-reactive hydrophilic surface was changed through substituting the carboxylic acid group (-COOH) in the 11-MUA molecule using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrocholide (EDAC). The formation of the self-assembled protein G layer on the Au substrate and binding of the antibody and antigen were investigated using SPR spectroscopy, while the surface topographies of the fabricated thin films were analyzed using atomic force microscopy (AFM). A fabricated monoclonal antibody (Mab) layer was applied for detecting E. coli O157:H7. As a result, a linear relationship was achieved between the pathogen concentration and the SPR angle shift, plus the detection limit was enhanced up to 10$^2$ CFU/mL.

• Journal of Korean Dental Science
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• v.4 no.2
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• pp.52-58
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• 2011

Purpose: Modifications of implant design have been related to improving initial stability. The purpose of this study was to investigate their respective effect on initial stability between two tapered implant systems (self-tapping vs. non-self-tapping) in medium density bone using three different analytic methods. Materials and Methods: Self-tapping implant (GS III$^{(R)}$; Osstem Implant Co., Busan, Korea) and non-self-tapping implant (Replace Select$^{(R)}$; Nobel Biocare, G$\H{o}$teborg, Sweden) were investigated. In Solid rigid polyurethane blocks of artificially simulated Quality 2 bone, each of the 5 implants was inserted according to the manufacturer's instructions for medium-bone drilling protocol. Evaluation of initial stability was carried out by recording the maximum insertion torque (IT) and performing the resonance frequency analysis (RFA), and the pull-out test. Results: The IT and RFA values of self-tapping implant were significantly higher than those of non self-tapping implant (P=.009 and P=.047, respectively). In the pull-out values, no significant differences were found in implants between two groups (P=.117). Within each implant system, no statistically significant correlation was found among three different outcome variables. Conclusions: These findings suggest that design characteristics of implant geometry significantly influence the initial stability in medium bone density.

• Journal of Biosystems Engineering
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• v.27 no.3
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• pp.227-234
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• 2002

This study was conducted to nondestructively estimate the volumetric information of peach and pit and to visualize the 3D information of internal structure from magnetic resonance imaging(MRI) data. Bruker Biospec 7T spectrometer operating at a proton reosonant frequency of 300 MHz was used for acquisition of MRI data of peach. Image processing algorithms and visualization techniques were implemented by using MATLAB (Mathworks) and Visualization Toolkit(Kitware), respectively. Thresholding algorithm and Kohonen's self organizing map(SOM) were applied to MRI data fur region segmentation. Volumetric information were estimated from segemented images and compared to the actual measurements. The average prediction errors of peach and pit volumes were 4.5%, 26.1%, respectively for the thresholding algorithm. and were 2.1%, 19.9%. respectively for the SOM. Although we couldn't get the statistically meaningful results with the limited number of samples, the average prediction errors were lower when the region segmentation was done by SOM rather than thresholding. The 3D visualization techniques such as isosurface construction and volume rendering were successfully implemented, by which we could nondestructively obtain the useful information of internal structures of peach.

• Nuclear Engineering and Technology
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• v.49 no.6
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• pp.1269-1286
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• 2017

This paper presents a newly developed resonance self-shielding method based on Tone's method in $APOLLO3^{(R)}$ for fast and thermal reactor calculations. The new method is based on simplified models, the narrow resonance approximation for the slowing down source and Tone's approximation for group collision probability matrix. It utilizes mathematical probability tables as quadrature formulas in calculating effective cross-sections. Numerical results for the ZPPR drawer calculations in 1,968 groups show that, in the case of the double-column fuel drawer, Tone's method gives equivalent precision to the subgroup method while markedly reducing the total number of collision probability matrix calculations and hence the central processing unit time. In the case of a single-column fuel drawer with the presence of a uranium metal material, Tone's method obtains less precise results than those of the subgroup method due to less precise heterogeneous-homogeneous equivalence. The same options are also applied to PWR UOX, MOX, and Gd cells using the SHEM 361-group library, with the objective of analyzing whether this energy mesh might be suitable for the application of this methodology to thermal systems. The numerical results show that comparable precision is reached with both Tone's and the subgroup methods, with the satisfactory representation of intrapellet spatial effects.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 2001.12a
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• pp.251-254
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• 2001

본 논문에서는 Self Organizing Map을 이용하여 fMRI data를 분석해 보았다. fMRl (functional Magnetic Resonance Imaging)는 인간의 뇌에 대한 비 침투적 연구 방법 중 최근에 각광받고 있는 것이다. Motor task를 수행하고 있는 피험자로부터 image data를 얻어내어 SOM을 적용하여 clustering한 결과 motor cortex 영역이 뚜렷하게 clustering 되었음을 알 수 있었다.

• Journal of Sensor Science and Technology
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• v.19 no.2
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• pp.142-148
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• 2010

We have compared and investigated the detection capabilities of antibody of immunoglobulin G(anti-IgG) immobilized by protein G and N-hydroxysuccinimide(NHS) at the end of the self-assembled monolayer(SAM). Surface plasmon resonance(SPR) sensor has been utilized to measure the interaction between biomolecules. After formation of the protein G and SAM, anti-IgG, bovine serum albumin(BSA) and IgG has been sequently injected. Through the reponse of the SPR, we can conclude that the protein G immobilized anti-IgG better than the SAM. In addition, IgG detection capability of the anti-IgG immobilized by the protein G showed better performance compared with that immobilized by the SAM.

• Analytical Science and Technology
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• v.18 no.6
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• pp.460-468
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• 2005

The biotin-avidin complex, as a model recognition system, has been constructed through N-hydroxysuccinimide(NHS) reaction on a variety of substrates such as a smooth Au film, electrochemically roughened Au electrode and chemically modified mica. Stepwise self-assembled monolayers (SAMs) of biotin-avidin system were characterized by surface-enhanced resonance Raman scattering (SERRS) spectroscopy, atomic force microscopy (AFM) and surface plasmon resonance (SPR). A strong SERRS signal of rhodamine tags labeled in avidin from the SAMs on a roughened gold electrode indicated the successful complex formation of stepwise biotin-avidin recognition system. AFM images showed the circular shaped avidin aggregates (hexamer) with ca. $60{\AA}$ thick on the substrate, corresponding to one layer of avidin. The surface coverage and concentration of avidin molecules were estimated to be 90% and $7.5{\times}10^{-12}mol/cm^2$, respectively. SPR technique allowed one to monitor the surface reaction of the specific recognition with high sensitivity and precision.

• Proceedings of the Korean Magnetic Resonance Society Conference
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• 2002.08a
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• pp.85-85
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• 2002

Lithiated transition metal oxides such as LiMn2O4, Lil-xMnO$_2$, LiNiO$_2$, LiCoO$_2$, and their solid solution phases are used as cathode materials for lithium rechargeable batteries. We prepared the cathode materials using a novel eutectic self-mixing method without any artificial mixing procedures. This method provides an extraordinarily simple way to make the cathode materials, and it is possible to prepare at very low temperature such as 25$0^{\circ}C$. Furthermore, the cathode materials produced have discharge capacities that are much better than cathode materials prepared by previously reported synthetic methods. The spontaneous and homogeneous mixing is verified by $^{7}$ Li magic-angle-spinning (MAS) NMR spectroscopy.

• The Pure and Applied Mathematics
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• v.15 no.4
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• pp.407-414
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• 2008

In this paper, we study the self-adjoint second order boundary value problem with integral boundary conditions: (p(t)x'(t))'+f(t,x(t))=0, t $${\in}$$ (0,1), x'(0)=0, x(1) = $${\int}_0^1$$ x(s)g(s)ds. A new result on the existence of positive solutions is obtained. The interesting points are: the first, we employ a new tool-the recent Leggett-Williams norm-type theorem for coincidences; the second, the boundary value problem is involved in integral condition; the third, the solutions obtained are positive.

• Proceedings of the KIEE Conference
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• 1992.07b
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• pp.1100-1103
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• 1992

Two different resonance inverters used as fluorescent lamp ballast are based on the self oscillation at the series resonance of the circuit components. Each circuit is simulated on a computer in order to explain its function and the variation of the circuit variables for each of the circuit elements. Experimental results have bean carried out on the unsymmetrical scheme to indicate the voltage and current of the fluorescent lamp operation at the high frequency.