Journal of Sensor Science and Technology (센서학회지)

The Korean Sensors Society (한국센서학회)
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A comparison of detection capabilities of anti-IgG immobilizedby protein G and NHS

Protein G와 NHS를 이용하여 고정한 anti-IgG의 검출 성능 비교

  • Sin, Eun-Jung (Department of Biomedical Engineering, Catholic University of Daegu) ;
  • Sohn, Young-Ho (School of Electronic Engineering, Communication Engineering and Computer Science, Yeungnam University) ;
  • Sohn, Young-Soo (Department of Biomedical Engineering, Catholic University of Daegu)
  • 신은정 (대구가톨릭대학교 의공학과) ;
  • 손영호 (영남대학교 전자정보공학부) ;
  • 손영수 (대구가톨릭대학교 의공학과)
  • Received : 2010.01.12
  • Accepted : 2010.03.15
  • Published : 2010.03.31
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Abstract

We have compared and investigated the detection capabilities of antibody of immunoglobulin G(anti-IgG) immobilized by protein G and N-hydroxysuccinimide(NHS) at the end of the self-assembled monolayer(SAM). Surface plasmon resonance(SPR) sensor has been utilized to measure the interaction between biomolecules. After formation of the protein G and SAM, anti-IgG, bovine serum albumin(BSA) and IgG has been sequently injected. Through the reponse of the SPR, we can conclude that the protein G immobilized anti-IgG better than the SAM. In addition, IgG detection capability of the anti-IgG immobilized by the protein G showed better performance compared with that immobilized by the SAM.

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References

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