• Journal of Practical Engineering Education
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• v.14 no.3
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• pp.543-553
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• 2022

A collaborative robot(Cobot) is one of the production systems presented in the 4th industrial revolution and are systems that can maximize efficiency by combining the exquisite hand skills of workers and the ability of simple repetitive tasks of robots. Also, research on the development of an efficient interface method between the worker and the robot is continuously progressing along with the solution to the safety problem arising from the sharing of the workspace. In this study, a method for controlling the robot by recognizing the worker's hand signal was presented to enhance the convenience and concentration of the worker, and the safety of the worker was secured by introducing the concept of a safety zone. Various technologies such as robot control, PLC, image processing, machine learning, and ROS were used to implement this. In addition, the roles and interface methods of the proposed technologies were defined and presented for using educational media. Students can build and adjust the educational media system by linking the introduced various technologies. Therefore, there is an excellent advantage in recognizing the necessity of the technology required in the field and inducing in-depth learning about it. In addition, presenting a problem and then seeking a way to solve it on their own can lead to self-directed learning. Through this, students can learn key technologies of the 4th industrial revolution and improve their ability to solve various problems.

• Molecules and Cells
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• v.46 no.9
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• pp.545-557
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• 2023

Sphingomyelinase (SMase) catalyzes ceramide production from sphingomyelin. Ceramides are critical in cellular responses such as apoptosis. They enhance mitochondrial outer membrane permeabilization (MOMP) through self-assembly in the mitochondrial outer membrane to form channels that release cytochrome c from intermembrane space (IMS) into the cytosol, triggering caspase-9 activation. However, the SMase involved in MOMP is yet to be identified. Here, we identified a mitochondrial Mg²⁺-independent SMase (mt-iSMase) from rat brain, which was purified 6,130-fold using a Percoll gradient, pulled down with biotinylated sphingomyelin, and subjected to Mono Q anion exchange. A single peak of mt-iSMase activity was eluted at a molecular mass of approximately 65 kDa using Superose 6 gel filtration. The purified enzyme showed optimal activity at pH of 6.5 and was inhibited by dithiothreitol and Mg²⁺, Mn²⁺, Ni²⁺, Cu²⁺, Zn²⁺, Fe²⁺, and Fe³⁺ ions. It was also inhibited by GW4869, which is a non-competitive inhibitor of Mg²⁺-dependent neutral SMase 2 (encoded by SMPD3), that protects against cytochrome c release-mediated cell death. Subfractionation experiments showed that mt-iSMase localizes in the IMS of the mitochondria, implying that mt-iSMase may play a critical role in generating ceramides for MOMP, cytochrome c release, and apoptosis. These data suggest that the purified enzyme in this study is a novel SMase.

• Korean Journal of Agricultural and Forest Meteorology
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• v.25 no.3
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• pp.172-181
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• 2023

In order to raise the self-sufficiency rate of domestic forage and obtain informations necessary for production of high-quality winter forage, forage yield and feeding value were analyzed in the above-ground parts of a winter wheat 'Cheongwoo' harvested at five different growth stages, and the appropriate harvest time was determined. The yield increased until 30 days after heading, and then decreased afterwards. The proportion of spike in the above-ground part was less than 40% until 30 days after heading, but increased to more than 60% afterward. At 30 days after heading, the protein content and relative feed value (RFV) also reached peaks, while acid detergent fiber (ADF) and neutral detergent fiber (NDF) were low. The mineral nutrient contents are within the standard range required for feeding ruminant livestock or do not exceed the maximum allowable level. Therefore, the best time to harvest above-ground parts of a winter wheat 'Cheongwoo' for use as a forage for feeding ruminant livestock is around 30 days after heading, when considering forage yield and feed values. As a result it would match well in time with the transplantation of rice seedlings or the sowing of rice-alternative field crops in the double cropping system.

• Animal Bioscience
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• v.37 no.3
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• pp.471-480
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• 2024

Objective: The objective of this study was to investigate the regulation relationship of Ten-eleven translocation 1 (Tet1) in DNA demethylation and the proliferation of primordial germ cells (PGCs) in chickens. Methods: siRNA targeting Tet1 was used to transiently knockdown the expression of Tet1 in chicken PGCs, and the genomic DNA methylation status was measured. The proliferation of chicken PGCs was detected by flow cytometry analysis and cell counting kit-8 assay when activation or inhibition of Wnt4/β-catenin signaling pathway. And the level of DNA methylation and hisotne methylation was also tested. Results: Results revealed that knockdown of Tet1 inhibited the proliferation of chicken PGCs and downregulated the mRNA expression of Cyclin D1 and cyclin-dependent kinase 6 (CDK6), as well as pluripotency-associated genes (Nanog, PouV, and Sox2). Flow cytometry analysis confirmed that the population of PGCs in Tet1 knockdown group displayed a significant decrease in the proportion of S and G2 phase cells, which meant that there were less PGCs entered the mitosis process than that of control. Furthermore, Tet1 knockdown delayed the entrance to G1/S phase and this inhibition was rescued by treated with BIO. Consistent with these findings, Wnt/β-catenin signaling was inactivated in Tet1 knockdown PGCs, leading to aberrant proliferation. Further analysis showed that the methylation of the whole genome increased significantly after Tet1 downregulation, while hydroxyl-methylation obviously declined. Meanwhile, the level of H3K27me3 was upregulated and H3K9me2 was downregulated in Tet1 knockdown PGCs, which was achieved by regulating Wnt/β-catenin signaling pathway. Conclusion: These results suggested that the self-renewal of chicken PGCs and the maintenance of their characteristics were regulated by Tet1 mediating DNA demethylation through the activation of Wnt4/β-catenin signaling pathway.

• The Journal of the Convergence on Culture Technology
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• v.10 no.2
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• pp.499-503
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• 2024

Broadcasting programs are provided to various media such as Internet replay, OTT, and IPTV services as well as self-broadcasting. In this case, it is very important to provide keywords for search that represent the characteristics of the content well. Broadcasters mainly use the method of manually entering key keywords in the production process and the archive process. This method is insufficient in terms of quantity to secure core metadata, and also reveals limitations in recommending and using content in other media services. This study supports securing a large number of metadata by utilizing closed caption data pre-archived through the DTV closed captioning server developed in EBS. First, core metadata was automatically extracted by applying Google's natural language AI technology. The next step is to propose a method of finding core metadata by reflecting priorities and content characteristics as core research contents. As a technology to obtain differentiated metadata weights, the importance was classified by applying the TF-IDF calculation method. Successful weight data were obtained as a result of the experiment. The string metadata obtained by this study, when combined with future string similarity measurement studies, becomes the basis for securing sophisticated content recommendation metadata from content services provided to other media.

• Journal of the Korean Association of Geographic Information Studies
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• v.27 no.1
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• pp.29-40
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• 2024

Recently, the technology of autonomous driving, one of the core of the fourth industrial revolution, is developing, but sensor-based autonomous driving is showing limitations, such as accidents in unexpected situations, To compensate for this, HD-map is being used as a core infrastructure for autonomous driving, and interest in the public and private sectors is increasing, and various studies and technology developments are being conducted to secure the latest and accuracy of HD-map. Currently, NGII will be newly built in urban areas and major roads across the country, including the metropolitan area, where self-driving cars are expected to run, and is working to minimize data error rates through quality verification. Therefore, this study analyzes the spatial relationship of reference objects in the attribute structuring process for rapid and accurate renewal and production of HD-map under construction by NGII, By applying the attribute input automation methodology of the reference object in which spatial relations are established using the library of open source-based PyQGIS, target sites were selected for each road type, such as high-speed national highways, general national highways, and C-ITS demonstration sections. Using the attribute automation tool developed in this study, it took about 2 to 5 minutes for each target location to automatically input the attributes of the spatial relationship reference object, As a result of automation of attribute input for reference objects, attribute input accuracy of 86.4% for high-speed national highways, 79.7% for general national highways, 82.4% for C-ITS, and 82.8% on average were secured.

• Korean Journal of Soil Science and Fertilizer
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• v.15 no.1
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• pp.2-15
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• 1982

In this report, a total domestic demand for major commercial fertilizer for crop production in Korea up to 1996 was estimated. The agricultural products and area for demand for both 1982 and 1986 was quoted from the estimate of the 5th Five-year Economic plan. And the demands estimated for 1991 and 1996 reflected possible changes of diet from cereal to meat and their indirect effects on the increase of cereal consumption. As the advanced countries followed, consequently, the demands for soybean, corn and other feed grains were expected to be increased as well as the land for growing those crops. 1. Total annual demands for nitrogen, phosphorus and potassium fertilizers were estimated 1,050,000M/T, 1,110,000M/T, 1,280,000M/T and 1,010,000M/T for the year 1982, 1986, 1991, and 1996 respectively. 2. It was assumed that there would be difficulties in self-sufficiency of grains at the cost of the maximum utilization of land and fertilizers in 1996. 3. It was clear that the increase of the productivity per unit area is possible by improving the conditions of arable land which could resulted a self-sufficiency of food in Korea. As a consequence, the demand for fertilizers at that time would exceed the level of estimates. 4. The recent decrease in demand for commercial fertilizers (currently estimated 850,000M/T) was due to an inadequate application of fertilizers for respective crop reqirement. This inadequacy should be checked and encouraged the consumptions of fertilizers to be increased by supporting the price of grain.

• (The) Research of the performance art and culture
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• no.32
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• pp.489-524
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• 2016

This study examines the birth of a genre, the $Sekky{\bar{o}}bushi$ Misemono Opera, focusing on how it accepted and modernized Katarimono $Sekky{\bar{o}}bushi$. Unlike earlier studies, it argues that Terayama was clearly different from other first-generation Angura artists, in that he rebirthed the medieval story $Sekky{\bar{o}}bushi$ as a modern Misemono Opera. Shintokumaru (1978) was directed by Terayama $Sh{\bar{u}}ji$, a member of the first generation of Japan's 1960s Angura Theatre Movement. It takes as its subject the Katarimono $Sekky{\bar{o}}bushi$ Shintokumaru, a story set to music that can be considered an example of the modern heritage of East Asian storytelling. $Sekky{\bar{o}}$ Shintokumaru is set in Tennoji, Japan. The title character Shintoku develops leprosy as a result of his stepmother's curse and is saved through his fiancee Otohime's devoted love and the spiritual power of the Bodhisattva Avalokitesvara. In this work, Terayama combined the narrative style of $Sekky{\bar{o}}bushi$ with J.A. Caesar's shamanistic rock music and gave it the subtitle 'Misemono Opera by $Sekky{\bar{o}}bushi$'. He transforms its underlying theme, the principle of goddesses and their offspring in a medieval religious world and the modori (return) instinct, into a world of mother-son-incest. Also, the pedestrian revenge scene from $Sekky{\bar{o}}bushi$ is altered to represent Shintokumaru as a drag queen, wearing his stepmother's clothes and mask, and he unites sexually with Sensaku, his stepbrother, and ends up killing him. The play follows the cause and effect structure of $Sekky{\bar{o}}bushi$. The appearance of katarite, a storyteller, propelling the narrative throughout and Dr. Yanagida Kunio is significant as an example of the modern use of self-introduction as a narrative device and chorus. Terayama $Sh{\bar{u}}ji^{\prime}s$ memories of desperate childhood, especially the absence of his father and the Aomori air raids, are depicted and deepened in structure. However, seventeen years after Terayama's death, the version of the play directed by Ninagawa Yukio-based on a revised edition by Kishida Rio, who had been Terayama's writing partner since the play's premier-is the today the better-known version. All the theatrical elements implied by Terayama's subtitle were removed, and as a result, the Rio production misses the essence of the diverse experimental theatre of Terayama's theatre company, $Tenj{\bar{o}}$ Sajiki. Shintokumaru has the narrative structure characteristic of aphorism. That is, each part of the story can stand alone, but it is possible to combine all the parts organically.

• (The) Research of the performance art and culture
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• no.41
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• pp.257-284
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• 2020

Goseong Ogwangdae started academic research in the late 1950s and was designated as a national intangible cultural asset in 1964. From the time of the academic survey to the time of designation, it was recorded as using paper masks, but when the recording image was filmed in 1965, it was already changed to wooden masks. In 1960, before being designated as an intangible cultural asset, the number of masks, which was 9 points, gradually increased to 19 points in 1964. It is necessarily included in the leper, Chorani, Malttuki, Cheongbo-Yangyang, Halmi, and Jemilju, but the character of the yangban is not yet clearly differentiated. Hwangbongsa and Sangju appeared as bare faces, and consumption, milling, Cheongbo-Yangyang are used together with Bibi and inspiration. It can be guessed that Bibi was not the appearance of a foreign object with horns as it is now, considering that Bibi and Madangsoi were used together. Since 1965, shortly after the designation, the whole of the Goseong Ogwangdae mask has been changed to a wooden mask. All the characters except for resident, courtyard, and top-of-the-line are wearing masks. Bibi, Hongbaek, and service masks have never appeared until 1964. The Yangban was changed to the closing ceremony with six people in the order of Won-Yangban, Baekje, Heukje, Cheongje, Hongbaek, and Jonggadoryong. Starting in 1969, the mask enters the stable period where the kind is the same as the present. Bibi-Yangban uses both the Won-Yangban and the Jemilju uses the Somu, but all other characters use the individual mask to use a total of 18 masks. The Yangbans are clearly differentiated, and a total of seven Yangban appear. The reason why the change in the type of mask and the expression of material is so large is that the first generation of mask makers died and the tradition of mask production was cut off, but there is also a cause of the extreme change in the environment of the drama that the performers who joined after the designation had to face. Also, it is closely related to the change of the times when the meaning and weight of masking in masking has changed. At that time, the performers were not so tied to the current concept of 'original form' that they preserved the appearance of the designated time. Originally, Goseong Ogwangdae was centered on improvisation dance, not the formalized dance as it is now, and there was a certain fluid aspect in the retelling, so it was flexible in the use of masks even before the designation of cultural assets. Strict rules did not apply in the details, as it was a self-sufficient play by the performers, not an offer event. The form and contents of this fluid play are changed to preparation for the performance while preparing for the folk art contest. As the subject of the contest in self-sufficient play, dance, costumes, and props became more and more colorful as well as dancing, costumes, and props. As a result, participation in the contest brought about changes in the overall performance and changed the mask, which was accepted within the preservation society.

• Journal of The Korean Society of Grassland and Forage Science
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• v.42 no.1
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• pp.17-25
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• 2022

This study was carried out the estimation on consumption patterns and consciousness of domestic forage for improvement of the quality of domestic forage. Although the cultivated area in South Korea of forage has increased significantly compared to the past, the self-sufficiency rate of domestic forage has increased to around 80% since 2010. Also, livestock farmers prefer to use import forage than domestic due to convenience of use. In Korean beef farms, the ratio of import to domestic forage was higher in domestic forage (import forage 3 : domestic forage 7). In the method of securing domestic forage, purchase of forage (55.6%) was higher than self-cultivation of forage (44.4%). The ratio of use by bailing type was shown in the order of rice staw rice straw (50.5%), domestic hay (15%), imported hay (12.5%), and total mixed ratio (10.7%). The preference of forage was in the order of amount of foreign matter, moisture content, price, feed value in Korean native cattle farm. The result of satisfaction with domestic and import forage showed that the satisfaction of domestic forage price was higher than import forage, while the moisture content and foreign matter of forage were lower than import forage. In addition, in the results of the satisfaction and importance of domestic roughage compared to imported roughage, satisfaction with imported roughage was generally high in all items except for price. As a result, in order to improve the satisfaction of domestic forage in Korean native cattle farm, it is necessary to minimize foreign matter in forage and increase hay production for moisture content uniform in forage.