• Applied Microscopy
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• v.41 no.2
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• pp.99-107
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• 2011

Light microscope, and scanning and transmission electron microscopy were used to investigate the fine structure of sperm of the Korea squirrel, Tamias sibiricus. The sperm head of T. sibiricus was paddle in shape. The total length of T. sibiricus sperm was 67.8 ${\mu}m$. The length of sperm head was 7.8 ${\mu}m$, and the tail (60.0 ${\mu}m$) was consisted of four major segments: the neck (1.0 ${\mu}m$), middle (8.0 ${\mu}m$), principal (48.5 ${\mu}m$) and end piece (2.5 ${\mu}m$), respectively. Especially, the length of the middle piece is short, and end piece was very shorter than those of other rodents. The post-nuclear cap was occupied about a fifth of nucleus. The equatorial segment is located between the post-nuclear cap segment and acrosomal cap on the nuclear surface. Nine segmented columns were surrounded by the mitochondria, and numbers of gyres of mitochondria were 26. One segmented column was consisted ten to twelve knobs, and each of segmented column in the neck region connected with the nine outer dense fiber in the middle piece. Numerous satellite-like fibers were scattered around the segmented columns. Nos. 1, 5 and 6 of the outer dense fibers in the middle piece were larger than the others. A fibrous sheath and longitudinal column of the principal piece were in evidence, but the fibrous sheath and longitudinal column was not seen at the end piece. In conclusion, the structural features of sperm head and tail may be useful information to patterns of sperm evolution and classification of species.

• Structural Engineering and Mechanics
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• v.43 no.2
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• pp.147-161
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• 2012

This paper presents a curvature method for analysis of beam-columns with different materials and arbitrary cross-section shapes and subjected to combined biaxial moments and axial load. Both material and geometric nonlinearities (the p-delta effect in this case) were incorporated. The proposed method considers biaxial curvatures and uniform normal strains of discrete cross-sections of beam-columns as basic unknowns, and seeks for a solution of the column deflection curve that satisfies force equilibrium conditions. A piecewise representation of the beam-column deflection curve is constructed based on the curvatures and angles of rotation of the segmented cross-sections. The resulting bending moments were evaluated based on the deformed column shape and the axial load. The moment curvature relationship and the beam-column deflection calculation are presented in matrix form and the Newton-Raphson method is employed to ensure fast and stable convergence. Comparison with results of analytic solutions and eccentric compression tests of wood beam-columns implies that this method is reliable and effective for beam-columns subjected to eccentric compression load, lateral bracings and complex boundary conditions.

• Applied Microscopy
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• v.29 no.1
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• pp.1-10
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• 1999

The aim of the present study was to investigate with transmission electron misroscope the comparative morphology of epididymal spermatozoa in two species of the Korean insectivorous bats belonging to 'prolonged sperm storage' type (Rhinolophus ferrumequinum korai) and 'delayed implantation' type (Miniopterus schreibersi fuliginosus). Sperm head of the R. ferrumequinum korai was bullet shaped and that of M. schreibersi fuliginosus was spatula shaped. The nuclei of the sperm head of the R. ferrumequinum korai and M. schreibersi fuliginosus occupied two-third and a half of it, respectively. The segmented columns of R. ferrumequinum korai were about 12 to 14 in number, and those of the M. schreibersi fuligincsus were about 10 to 12. Particularly, a pile of the satellite fibers in middle piece of R. ferrumequinum korai remained the inner aspect of the outer dense fibers, but those of the M. schreibersi fuliginosus were not.

• Korean Journal Plant Pathology
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• v.14 no.6
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• pp.559-562
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• 1998

Odontoglossum ringspot virus (ORSV) was finally purified from ORSV-infected orchid plants by diethylaminoethyl (DEAE) cellulose anion exchange column chromatography. The virus was reliably eluted by potassium chloride at the concentration from 0.1 M to 0.13 M. Partial purification was done by solubilization with Triton X-100 (allkylphenoxypolyethoxy ethanol) and precipitation with polyethylene glycol (PEG; MW 8,000). The finally purified ORSV represented one distinct homogeneous band and the molecular weight of its capsid protein was about 17,500 Dalton in electrophoretic analysis. Electron microscopy showed not only intact particles ranged from 280 nm to 340 nm in length, but also segmented particles that final 140 nm to 220 nm and even disks. Enzyme-linked immunosorbent assay (ELISA) showed that final yield was 12 mg/100 g of the infected leaves. Bioassay demonstrated that the purified ORSV had the normal infectivity to orchid plants and Nicotiana glutionsa. Based on these data, anion exchange column chromatography could be efficiently applied to the purification of ORSV and other viruses similar to ORSV.

• Applied Microscopy
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• v.41 no.2
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• pp.117-122
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• 2011

Sperm morphology of Pipistrellus savii velox was examined by transmission electron microscope. The sperm head of P. savii velox was bullet in shape. The sperm head was 3.1 ${\mu}m$ in length, whose posterior 3.0 ${\mu}m$ was occupied by a nucleus with 1.8 ${\mu}m$ in width. The segmented columns were about 14~15 in number. The total number of mitochondrial gyres was 57. Number of 1, 5, and 6 of the outer dense fibers were larger than the others. A fibrous sheath and longitudinal column of the principal piece were evidence, but the fibrous sheath was not seen at the end piece. In the present study, the length of the sperm head of P. savii velox were very shorter than those of other bats.

• 한국전산유체공학회:학술대회논문집
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• 2008.03b
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• pp.197-200
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• 2008

The existing computer code to solve the air flow in a segmented constrictor-type arc-heated wind tunnel named ARCFLO4 is improved to accept an air-argon mixture as the working gas and to consider the separated injection of air and argon. The new version of the code is used to calculate the flows in the Aerodynamic Heating Facility of NASA Ames Research Center where argon concentration is relatively high. The calculation shows that argon tends to increase the diameter of the arc-column, increase ionization fraction, decrease thermal efficiency of the arc-heater, and push the ratio of the centerline-to-average enthalpy toward unity. The calculated operating characteristics of the arc-heater agree well with the experimental data.

• Applied Microscopy
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• v.41 no.1
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• pp.31-35
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• 2011

The fine structures of the sperm morphology in the Myotis daubentonii ussuriensis were observed by transmission electron microscope. The results showed that the sperm head revealed bullet shaped, the width was showed a slender more than toward the posterior region to anterior region of nucleus. The sperm head was about $4.5{\mu}m$ in length, being about $2.0{\mu}m$ in width. The nuclear length was $4.3{\mu}m$, occupied most of the sperm head. The nucleus and acrosome were separated by the apical body. The neck region was composed the basal plate, capitulum and segmented columns. The segmented columns were about 12 to 14 in number and connected with the outer dense fibers of the middle piece. The mitochondria sheath were arranged like the thread of a screw, and the total number of mitochondrial gyres were 57. The satellite fibers were observed irregularly among the outer dense fibers in the middle piece. Except the middle piece they are not observed in the principal and end pieces of the tail. In general, the tail show axoneme composed of a 9+2 microtubular pattern, and microtubules of the end piece were arranged irregularly.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.1
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• pp.393-401
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• 2013

Most traditional databases exploit record-oriented storage model where the attributes of a record are placed contiguously in hard disk to achieve high performance writes. However, for search-mostly datawarehouse systems, column-oriented storage has become a proper model because of its superior read performance. Today, flash memory is largely recognized as the preferred storage media for high-speed database systems. In this paper, we introduce fast column-oriented database model and then propose a new column-aware index management scheme for the high-speed column-oriented datawarehouse system. Our index management scheme which is based on enhanced $B^+$-Tree achieves high search performance by embedded flash index and unused space compression in internal and leaf nodes. Based on the results of the performance evaluation, we conclude that our index management scheme outperforms the traditional scheme in the respect of the search throughput and response time.

• Applied Microscopy
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• v.39 no.2
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• pp.89-99
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• 2009

To investigate the morphological characteristics of spermatozoa of the grey red-blacked vole (Clethrionomys rufocanus) belongings to the subfamily Cricetinae, subgenus Clethrionomys were examined by scanning and transmission electron microscopes. The sperm head of C. rufocanus was an ax or hatchet in shape with a curved single dorsal hook. The total length of C. regulus sperm was 95.8 ${\mu}m$. The length of sperm head was 7.8 ${\mu}m$, and the tail (88.0 ${\mu}m$) consisted of four major segments: the neck (1.0 ${\mu}m$), middle piece (22.0 ${\mu}m$), and principal piece plus end piece (65.0 ${\mu}m$), respectively. The segmented columns were about 10~12 in number. The number of gyres of mitochondria ranged from about 170 to 178. The post-nuclear cap occupied about a half of nucleus. The equatorial segment is located between the post-nuclear cap segment and acrosomal cap on the nuclear surface. Nos. 1, 5 and 6 of the outer dense fibers were larger than the others. A fibrous sheath and longitudinal column of the principal piece were in evidence, but the fibrous sheath was not seen at the end piece. In conclusion, the morphological structures of sperm head and tail may be useful information to patterning of sperm evolution and classifying of species.

• The KIPS Transactions:PartB
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• v.18B no.5
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• pp.255-264
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• 2011

In this paper, we propose a forward vehicle detection algorithm using column detection and bird's-eye view mapping based on stereo vision. The algorithm can detect forward vehicles robustly in real complex traffic situations. The algorithm consists of the three steps, namely road feature-based column detection, bird's-eye view mapping-based obstacle segmentation, obstacle area remerging and vehicle verification. First, we extract a road feature using maximum frequent values in v-disparity map. And we perform a column detection using the road feature as a new criterion. The road feature is more appropriate criterion than the median value because it is not affected by a road traffic situation, for example the changing of obstacle size or the number of obstacles. But there are still multiple obstacles in the obstacle areas. Thus, we perform a bird's-eye view mapping-based obstacle segmentation to divide obstacle accurately. We can segment obstacle easily because a bird's-eye view mapping can represent the position of obstacle on planar plane using depth map and camera information. Additionally, we perform obstacle area remerging processing because a segmented obstacle area may be same obstacle. Finally, we verify the obstacles whether those are vehicles or not using a depth map and gray image. We conduct experiments to prove the vehicle detection performance by applying our algorithm to real complex traffic situations.