Fine Structure of Sperm in the Korea Squirrel, Tamias sibiricus

한국산 다람쥐(Tamias sibiricus) 정자의 미세구조

  • Lee, Jung-Hun (Department of Science Education, Kyungnam University) ;
  • Park, Ki-Ryong (Department of Science Education, Kyungnam University)
  • 이정훈 (경남대학교 사범대학 과학교육과) ;
  • 박기룡 (경남대학교 사범대학 과학교육과)
  • Received : 2011.04.28
  • Accepted : 2011.06.20
  • Published : 2011.06.30

Abstract

Light microscope, and scanning and transmission electron microscopy were used to investigate the fine structure of sperm of the Korea squirrel, Tamias sibiricus. The sperm head of T. sibiricus was paddle in shape. The total length of T. sibiricus sperm was 67.8 ${\mu}m$. The length of sperm head was 7.8 ${\mu}m$, and the tail (60.0 ${\mu}m$) was consisted of four major segments: the neck (1.0 ${\mu}m$), middle (8.0 ${\mu}m$), principal (48.5 ${\mu}m$) and end piece (2.5 ${\mu}m$), respectively. Especially, the length of the middle piece is short, and end piece was very shorter than those of other rodents. The post-nuclear cap was occupied about a fifth of nucleus. The equatorial segment is located between the post-nuclear cap segment and acrosomal cap on the nuclear surface. Nine segmented columns were surrounded by the mitochondria, and numbers of gyres of mitochondria were 26. One segmented column was consisted ten to twelve knobs, and each of segmented column in the neck region connected with the nine outer dense fiber in the middle piece. Numerous satellite-like fibers were scattered around the segmented columns. Nos. 1, 5 and 6 of the outer dense fibers in the middle piece were larger than the others. A fibrous sheath and longitudinal column of the principal piece were in evidence, but the fibrous sheath and longitudinal column was not seen at the end piece. In conclusion, the structural features of sperm head and tail may be useful information to patterns of sperm evolution and classification of species.

한국산 다람쥐 정자의 형태적 특징을 알아보기 위하여 광학현미경과 주사 및 투과 전자현미경으로 관찰하였다. 다람쥐 정자머리의 모양은 주걱 모양의 형태를 취하고 있었다. 정자의 전체 길이는 67.8 ${\mu}m$이었으며, 머리의 길이는 7.8 ${\mu}m$, 꼬리는 60.0 ${\mu}m$이었다. 꼬리는 경부(1.0 ${\mu}m$), 중편부(8.0 ${\mu}m$), 주편부(48.5 ${\mu}m$) 그리고 미부(2.5 ${\mu}m$)로 각각 구성되어져 있었다. 특히, 본 연구에서 정자의 중편부도 짧지만 미부의 길이는 다른 설치류 종보다도 매우 짧은 것이 특징적이다. 첨체 후 덮개는 핵의 약 1/5을 점유하고 있으며, 적도절은 핵 상단의 첨체 후 덮개와 첨체 덮개사이에 위치하고 있었다. 9개의 분절주들은 미토콘드리아에 의해 둘러싸여져 있었다. 중편부의 미토콘드리아의 회전수는 26개 이었다. 한 개의 분절주는 10~12개의 마디로 구성되어져 있었으며, 이들 경부의 각각의 분절주는 중편부의 9개의 외측치밀 섬유와 제각기 결합되어 있었다. 특히 경부의 분절주와 분절주 사이에는 수많은 satellite 섬유와 흡사한 물질들이 존재하고 있었다. 중편부의 9개의 외측 치밀 섬유는 Nos. 1, 5, 6의 섬유가 다른 섬유들(2, 3, 4, 7, 8, 9) 보다도 크다. 섬유초와 종 지주는 주편부에는 존재하고 있었으며, 미부에는 섬유초와 종 지주는 존재하지 않았다. 이상의 결과에서 볼 때, 정자두부와 꼬리의 구조적인 특징들은 정자의 진화와 더불어 종을 분류하는데 있어서 매우 중요한 정보를 제공하리라 여겨진다.

Keywords

References

  1. Austin CR: Fertilization, early, cleavage and associated phenomena in the field vole (Microtus agrestis). J Anat 91 : 1-11, 1957.
  2. Breed WG: Further observations on spermatozoal morphology and male reproductive tract anatomy of Pseudomys and Notomys species (Mammalia: Rodentia). Trans Roy Soc S Aust 104 : 51-55, 1980.
  3. Breed WG: Variation in sperm morphology in the Austrian rodent genus, Pseudomys (Muridae). Cell Tissue Res 229 : 611-625, 1983.
  4. Breed WG: Sperm head structure in the Hydromyinae (Rodentia: Muridae); a further evolutionary development of the subacrosomal space in mammals. Gamete Res 10 : 31-44, 1984. https://doi.org/10.1002/mrd.1120100105
  5. Breed WG: Variation in sperm head morphology of muroid rodents of Africa: Phylogenetic implications. In: Jamieson BGM, Ausio J, Justine J-L, eds, Advances in spermatozoal phylogeny and taxonomy. Mem Mus Natn Hist Nat 166 : 409-420, 1995.
  6. Breed WG: The spermatozoon of Eurasian murine rodents: Its morphological diversity and evolution. J Morphol 261 : 52-69, 2004. https://doi.org/10.1002/jmor.10228
  7. Breed WG: Evolution of the spermatozooon in muroid rodents. J Morphol 265 : 271-290, 2005.
  8. Breed WG, Leigh CM: Distribution of filamentous actin in and around spermatids and in spermatozoa of Australian Conilurine rodents. Mol Reprod Dev 30 : 369-384, 1991. https://doi.org/10.1002/mrd.1080300412
  9. Breed WG, Sarafis V: On the phylogenetic significance of spermatozoal morphology and male reproductive anatomy in Australian rodents. Trans Roy Soc S Aust 103 : 127-135, 1979.
  10. Breed WG, Cox GA, Leigh CM, Hawkins P: Sperm head structure of a murid rodent from Southern Africa: The red veld rat Aethomys chrysophilus. Gamete Res 19 : 191-202, 1988. https://doi.org/10.1002/mrd.1120190209
  11. Breed WG, Idriss D, Oko RJ: Protein composition of ventral processes on the sperm head of Austrailian Hydromyine rodents1. Biol Reprod 63 : 629-634, 2000. https://doi.org/10.1095/biolreprod63.2.629
  12. Flaherty SP: Further ultrastructural observations on the sperm head of the plains mouse, Pseudomys austrailis (Rodentia: Muridae). Anat Rec 217 : 240-249, 1987. https://doi.org/10.1002/ar.1092170304
  13. Flaherty SP, Breed WG: The sperm head of the plains mouse, Pseudomy australis: Ultrastructure and effects of chemical treatments. Gamete Res 8 : 231-244, 1983. https://doi.org/10.1002/mrd.1120080304
  14. Flaherty SP, Breed WG: Formation of the ventral hooks on the sperm head of the plains mouse, Pseudomys australis. Gamete Res 17 : 115-129, 1987. https://doi.org/10.1002/mrd.1120170204
  15. Flaherty SP, Breed WG, Sarafis V: Localization of actin in the sperm head of the plains mouse, Pseudomys australis. J Exp Zool 225 : 497-500, 1983. https://doi.org/10.1002/jez.1402250318
  16. Flaherty SP, Winfrey VP, Olson GE: Localization of actin in mammalian spermatozoa: a comparison of eight species. Anat Rec 216 : 504-515, 1986. https://doi.org/10.1002/ar.1092160407
  17. Friend GF: The sperms of the British Muridae. Q J Microsc Sci 78 : 419-443, 1936.
  18. Jones RC: The utrastrucure of spermatozoa from some hystricomorph rodents. In: Afzelius BA, ed, The functional anatomy of the spermatozoon, pp. 251-258, Pergamon Press, Oxford, 1974.
  19. Jung TD, Lee JH: Seminiferous epithelium cycle in the Korean squirrel, Tamias sibiricus. J Exp Biomed Sci 10 : 275-283, 2004. (Korean)
  20. Jung TD, Lee JH, Kim SS: Spermiogenesis in the Korean squirrel, Tamias sibiricus. Korean J Electr Micros 34 : 159-170, 2004. (Korean)
  21. Hirth HF: The spermatozoa of some North American bats and rodents. J Morph 106 : 77-83, 1960. https://doi.org/10.1002/jmor.1051060103
  22. Knepp TH: A quantitative study of the testes of ten mammals. Proc Penn Sci 10 : 58-62, 1936.
  23. Lee JH, Mori T: Ultrastructural observations on the sperm of two Apodemus species, Apodemus agrarius coreae and Apodemus speciosus peninsulae, in Korea. J Fac Agr Kyushu Univ 51 : 125-133, 2006.
  24. Lee JH : Ultrastructural observation on the sperm of the grey redblacked vole, Clethrionomys rufocanus. Kor Soc Micros 39 : 89-99, 2009. (Korean)
  25. Oh YK, Mori T, Uchida TA: Spermiogenesis in the japanese greater horseshoe bat, Rhinolophus ferrumequnium nippon. J Fac Agr Kyushu Univ 29 : 203-209, 1985.
  26. Roldan ESR, Vitullo AD, Gomendio M: Sperm shape and size: Evolutionary processes in mammals. In: Baccetti B, ed, Comparative spermatology 20 years after, pp. 1001-1010, Serono Symposia Publication from Raven Press, 1991.
  27. Sarafis V, Lambert RW, Breed WG: Sperm head morphology of the plains mouse Pseudomys australis. J Reprod Fert 61 : 399-401, 1981. https://doi.org/10.1530/jrf.0.0610399