• Korean Journal of Medicinal Crop Science
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• v.27 no.3
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• pp.167-172
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• 2019

Background: Angelica acutiloba Kitagawa belongs to the flowering plant family Apiaceae. Its young leaves are consumed as a vegetable, and its roots have medicinal properties. This study was conducted to analyze the seed germination characteristics of A. acutiloba according to its ripening stages. Methods and Results: The seeds were harvested from a research farm managed by the Department of Herbal Crop Research, Rural Development Administration in 2018 and were divided into six groups according to their specific gravities. In particular, we studied the effect of harvesting periods and umbel orders on seed characteristics. The results showed that the rates of germination, emergence, and early growth improved as the specific gravity of the seeds increased. In addition, the germination rates of the seeds harvested in mid July and early August were significantly higher than those harvested in the other seasons, and the seeds obtained from the first floret had the greatest mass and weight. Conclusions: In this study, we demonstrated that it is possible to improve the germination rate by appropriate selection of seeds and harvesting period both of which are closely related to seed maturity.

• Korean Journal of Medicinal Crop Science
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• v.22 no.6
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• pp.435-441
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• 2014

This study was conducted to investigate the quality of seeds, the germination rates and the days required for germination, to examine the patterns of protein expressions during the germination and to improve the techniques of managing and storing seeds and viability of the seeds of Lithospermum erythrorhizon Sieb. et Zucc. After collecting and harvesting seeds, they were classified to white and brown colors of seed coat through testing their seed size, weight, and quality. The germination rates, the days required for germination, and the protein expressions were examined with different colors of seed coats, storing temperatures and durations by treating the different plant growth regulators and primings. One hundred seed weight of white color was heavier about 1.17 g than those of brown one about 0.81 g. The germination rates in white color of seed coat was higher, 3.05 ~ 5.75%, than those in brown one. Its rates were decreased with getting longer in storage durations. There was no big differences on germination rates between storage temperatures. The plant growth regulator of $GA_3$ and Kinetin was affected to improve the seed germination. $GA_3$ increased the seed germination clearly at 25 ppm level, while kinetin increased it gradually from 25 to 100 ppm levels. In germination by seed primings, PEG6000 made higher germination rate with increasing their levels, whereas $KNO_3$ increased the germination until 100 mM level and then decreased it with 200 mM unlike PEG6000. The protein expressed during the seed germination were appeared more and clearer bands in the seed after germination, especially 20 ~ 30 kDa, compared to those in the seed before germination. These results showing more and clearer bands were positively related to the germination rates which were different by seed colors, storage temperatures and durations, and plant growth regulators and primings.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.19 no.2
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• pp.164-168
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• 2014

To determine the optimal storage method and longest possible storage period of Zostera marina seeds, we examined post-storage germination rates using different storage methods and periods for Z. marina seeds harvested in southern coast of Korea. Storage methods included in seawater at room temperature with air supply, seawater at $4^{\circ}C$, seawater at $30^{\circ}C$, an aquarium with continuous seawater circulation, seawater at room temperature in an airtight tank, a refrigerator at $-20^{\circ}C$, and a desiccator at room temperature. Germination rates of Z. marina seeds stored in seawater at $4^{\circ}C$ showed highest germination (52.0%), whereas those of seeds stored in seawater at room temperature and an aquarium were 27.4% and 17.7%, respectively. But the seeds stored in seawater at $30^{\circ}C$, a refrigerator, and a desiccator did not germinate. Storage periods were 10, 20, 30, 60, 180 and 240 days of storage. Z. marina seeds maintained germination rates of 46.4~52.4% until 10~60 days of storage, but showed rapidly decreasing germination rates after then and no germination after 240 days.

• Journal of The Korean Society of Grassland and Forage Science
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• v.13 no.1
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• pp.31-37
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• 1993

It is important to improve the germination of grass seeds because they are poor germination under water stress. This experiment was conducted to investigate the response of seeds pre-treated individually with Polyethylene glycol 6000 (PEG) and growth regulators(GA$_3$, Kinetin, NAA) on the germination characteristics when two levels of osmotic potential (0, -5 bar) were put to seeds of Orchardgrass. In the untreated seeds, the total germination rate was decreased under water stress, and the mean germination time was delayed. Priming of Orchardgrass seeds using PEG or GA$_3$ resulted in improved germination under water stress(-5 bar), whereas the opposite was true of kinetin or NAA. The response of seeds primed in solutions of either -15 bar PEG or 100 ppm GA$_3$ was particulary marked compared with the untreated seeds. It is suggested that PEG and GA$_3$ may have positive regulatory effects in triggering the system for water stress alleviation. But the inhibitory effect of the water stress was not completely removed by allowing the seeds to pre-treat in solution of PEG or GA$_3$.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.22 no.3
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• pp.69-76
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• 2017

Eelgrass, Zostera marina is a marine flowering plant that grows in submerged habitats for most of its lifetime, and experiences desiccation stress when exposed to air. Although the desiccation stress observed in adult eelgrass has been frequently studied, there has been little research on desiccation stress in eelgrass seeds. To survey desiccation stress in eelgrass seeds, we studied the germination rate and moisture content upon desiccation caused by exposure to air. The germination rate of the eelgrass seeds exposed to air for 1 hour at $20^{\circ}C$ and 50% relative humidity decreased markedly, and it decreased consistently as the exposure time increased. Eelgrass seeds exposed to air for longer than 11 hours did not germinate. As the exposure time of eelgrass seeds to air increased, the germination rate and moisture content decreased, showing a negative correlation. In addition, eelgrass seeds exposed to air at $30^{\circ}C$ showed significantly lower germination rates than those exposed to air at $10^{\circ}C$ or $20^{\circ}C$. Our results revealed that desiccation causes a decrease in the germination of eelgrass seeds, which will provide useful information for eelgrass habitat restoration using seeds.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.33 no.1
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• pp.54-58
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• 1988

To define natural embryo growth and germination of the Korean ginseng (Panax ginseng C.A.Meyer) seed, freshly harvested and depulped seeds were seeded on nursery bed at Aug. 5 without stratification. At 100 days after seeding, embryo/endosperm length ratio of the seeds in nursery bed was higher than that of the seeds which were stratified with conventional method for 100 days. And also there was no significant difference between dehiscence ratio of the seeds in nursery bed and that from stratification with sand. The germination ratio of the ginseng seeds seeded without stratification at Aug. 5 was about 57% in average of 2 years and was not significantly lower than that of the seeds seeded after stratification at Nov. 15. And there were also no significant differencies of plant growth after germination between the ginseng seedlings from two seeding methods.

• Horticultural Science & Technology
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• v.17 no.2
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• pp.136-137
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• 1999

This experiment was conducted to improve germination rate and growth of Lilium formolongi 'Neosan No. 2' seeds by priming. Seeds were immersed in the solutions of 200 mM $Ca(NO_3)_2$, 200 mM $KNO_3$ for 3 days and 100 mg/L $GA_3$ for 1 day at $17{\pm}1^{\circ}C$. For chilling treatment, seeds were kept in the low temperature chamber of $2{\pm}1^{\circ}C$ for 30 days. Germination percentage of seeds treated with $Ca(NO_3)_2$ or $KNO_3$ was higher than that of seeds with other treatments and control seeds to 10 days after seeding, but final germination percentage was not significantly different among all treatments including control. Early growth of seedlings treated with $Ca(NO_3)_2$ and $KNO_3$ were better than that of seedling with other treatments.

• Journal of Life Science
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• v.23 no.12
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• pp.1415-1419
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• 2013

Electron beam (EB) irradiation was tested to determine the dose required to eradicate plant pathogens, such as Botrytis cinerea and Agrobacterium rhizogenes, from the infected seeds without affecting the germination rate of the irradiated vegetable seeds, including crown daisy, cucumber, hot pepper, green onion, leaf lettuce, and radish seeds. EB irradiation of 1.5 kGy and 2 kGy was sufficient to kill 100% of hairy root disease bacteria and gray mold conidia, respectively. EB irradiation showed no effect or minimal effect on the germination rate of the crown daisy, cucumber, green onion, and radish seeds. However, the germination rate of the hot pepper and leaf lettuce seeds was significantly reduced by using 2 kGy of EB irradiation. Difference in susceptibility to EB irradiation appears not to be related to the weight of each seed, but to the intrinsic characteristic of each plant. Conclusively, EB irradiation might be a useful way to decontaminate crown daisy, cucumber, green onion, and radish seeds.

• Plant Resources
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• v.8 no.2
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• pp.167-170
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• 2005

This study was conducted to determine feasibility of production system of Perilla frutescens leaf-stem by fertilizing of Sta-Green in pots. Germination rate of Perilla frutescens seeds collected in 2002 was $7\%$, also germination rate of seeds collected in 2003 was $62\%$, while germination rate of seeds collected in 2004 was above $93\%$. Seed germination rate of Perilla frutescens collected in 2004 were higher than seed gathering in 2002. Especially, plant growth and yield of Perilla frutescens grown in pot(The pots was filled with soil mixtures of Sta-Green and Peat Moss mixed with 40:60 ratio.) was the highest. These results indicate that leaf and stem production of Perilla frutescens can be improved by fertilizing of Sta-Green in pots.

• Asian Journal of Turfgrass Science
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• v.15 no.1
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• pp.15-20
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• 2001

Two types of annual bluegrass have been reported, and those consist of annual type (Poa annua ssp. annua) and perennial type (Poa annua ssp. reptans). As a weed, annual bluegrasses are commonly found in putting greens and fairways in many golf courses. Due to its strong competitiveness such as tremendous seed reproduction rate a year, prostrate growth habit, and no herbicide availability, annual bluegrasses have been considered as one of the most hard-to-control weeds in turf management systems. A growth chamber study was conducted to determine seed dormancy and to understand seed germination characteristics of annual bluegrass (Poa annua ssp. annua). Freshly harvested seeds showed 80 and 55% germination at 30 and $35^{\circ}C$, respectively, indicating that the optimum temperature of annual bluegrass is $30^{\circ}C$. However, the seed germination occurred only under light condition at any given temperature. This result demonstrated that light is prerequisite for the seed germination, and no primary dormancy of annual bluegrass seed exists. Secondary seed dormancy induced by unfavorable temperatures and dark condition was broken through 4 to 6 wk-storage at $4^{\circ}C$ with moisture, and the stored seeds germinated at $20^{\circ}C$ even under the dark. In red and far-red light trial, fresh seeds resulted in 40% germination under red while no seed germination occurred under far-red light condition, indicating that phytochrome Pr and Pfr could be related to annual bluegrass seed germination. When the far-red light replaced the red the germination was recovered, but this reversibility did not reach to the germination level under the red light only. This result implied that other lights than red and far-red would play an important role on seed germination of annual bluegrass.