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Studies on Seed Germination Characteristics and Patterns of Protein Expression of Lithospermum erythrorhizon by Plant Growth Regulators and Seed Primings

생장조절제와 프라이밍 처리에 의한 지치종자의 발아특성과 단백질 발현 양상에 관한 연구

  • Kim, Do Hyun (Department of Plant Science, College of Agriculture Life & Environment Science, Chungbuk National University) ;
  • Ahn, Bok Ju (Department of Plant Science, College of Agriculture Life & Environment Science, Chungbuk National University) ;
  • An, Hee Jung (Department of Plant Science, College of Agriculture Life & Environment Science, Chungbuk National University) ;
  • Ahn, Young Sup (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Young Guk (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Park, Chun Geun (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Park, Chung Beom (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Cha, Seon Woo (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Song, Beom Heon (Department of Plant Science, College of Agriculture Life & Environment Science, Chungbuk National University)
  • 김도현 (충북대학교 농업생명환경대학 식물자원학과) ;
  • 안복주 (충북대학교 농업생명환경대학 식물자원학과) ;
  • 안희정 (충북대학교 농업생명환경대학 식물자원학과) ;
  • 안영섭 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김영국 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 박춘근 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 박충범 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 차선우 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 송범헌 (충북대학교 농업생명환경대학 식물자원학과)
  • Received : 2014.08.09
  • Accepted : 2014.10.13
  • Published : 2014.12.30

Abstract

This study was conducted to investigate the quality of seeds, the germination rates and the days required for germination, to examine the patterns of protein expressions during the germination and to improve the techniques of managing and storing seeds and viability of the seeds of Lithospermum erythrorhizon Sieb. et Zucc. After collecting and harvesting seeds, they were classified to white and brown colors of seed coat through testing their seed size, weight, and quality. The germination rates, the days required for germination, and the protein expressions were examined with different colors of seed coats, storing temperatures and durations by treating the different plant growth regulators and primings. One hundred seed weight of white color was heavier about 1.17 g than those of brown one about 0.81 g. The germination rates in white color of seed coat was higher, 3.05 ~ 5.75%, than those in brown one. Its rates were decreased with getting longer in storage durations. There was no big differences on germination rates between storage temperatures. The plant growth regulator of $GA_3$ and Kinetin was affected to improve the seed germination. $GA_3$ increased the seed germination clearly at 25 ppm level, while kinetin increased it gradually from 25 to 100 ppm levels. In germination by seed primings, PEG6000 made higher germination rate with increasing their levels, whereas $KNO_3$ increased the germination until 100 mM level and then decreased it with 200 mM unlike PEG6000. The protein expressed during the seed germination were appeared more and clearer bands in the seed after germination, especially 20 ~ 30 kDa, compared to those in the seed before germination. These results showing more and clearer bands were positively related to the germination rates which were different by seed colors, storage temperatures and durations, and plant growth regulators and primings.

Keywords

References

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