• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.23 no.2
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• pp.57-68
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• 2010

Objective : This study was performed to evaluate the effect of Lithospermum erythrorhizon extracts on the inflammatory cytokines gene expression by the bacteria of Propionibacterium acnes (P. acnes) which elicits acne in human monocytes, THP-1 cell line. Experiment : Cytotoxicity of Lithospermum erythrorhizon extracts was analyzed by XTT assay. Real time RT-PCR was applied to analyze the cytokines gene expressions of IL-8, MCP-1 and TNF-$\alpha$. Translocation of transcription factor NF-${\kappa}B$ from cytoplasm into nucleus was observed using immunocytochemistry and confocal microscopy. Results : Lithospermum erythrorhizon extracts did not show cytotoxicity as high as in $1,000\;{\mu}g/ml$ of concentration. Transcription levels of inflammatory cytokines, IL-8, MCP-1 and TNF-$\alpha$ were increased by P. acnes in THP-1 and Lithospermum erythrorhizon extracts decreased the upregulated transcription levels. Lithospermum erythrorhizon extracts significantly inhibited the translocation of NF-${\kappa}B$ into nucleus by P. acnes. Conclusion : This study suggests that Lithospermum erythrorhizon extracts have anti-inflammatory effects on P. acnes treated THP-1 as decreasing the mRNA expressions of IL-8, MCP-1 and TNF-$\alpha$. This anti-inflammatory effect of Lithospermum erythrorhizon extracts may be useful in therapeutic treatments for acne vulgaris.

• Journal of the Korean Society of Food Science and Nutrition
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• v.28 no.2
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• pp.359-364
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• 1999

The studies were carried out to investigate antimicrobial activity of mixed extracts of Lithospermum erythrorhizon and Scutellaria baicalensis G. and crab shell against lactic acid bacteria isolated from kimchi. The effects of Lithospermum erythrorhizon and Scutellaria baicalensis G. extracts and crab shell on shelf life of kimchi were also investigated. The growth of heterofermentative and homofer mentative lactic acid bacteria was inhibited by 95% ethanol extracts of Lithospermum erythrorhizon and Scutellaria baicalensis G. The pH of kimchi containing 1% of mixed extracts Lithospermum erythrorhizon. and Scutellaria baicalensis G., and crab shell was lower than control during fermentation for 25 days of 10oC. The viable cells of the kimchi samples were lower than that of control during fermentation. The sensory qualities of the kimchi samples were a little inferior to the control during shelf life of kimchi.

• The Journal of Pediatrics of Korean Medicine
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• v.18 no.1
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• pp.63-75
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• 2004

Objective: Atopic dermatitis has a close relationship with degranulation of mast cell and separation of histamine. As there was no experiment with herb, using Lithospermum erythrorhizon, We investigated experimental influence of Lithospermum erythrorhizon on degranulation of mast cell and separation of histamine in Sprague-Dawley rats. Methods: The SD rats were classified into three groups. One group was a normal one treated by normal saline before medical treatment. The other was a control group prescribed to Compound 48/80 before normal saline treatment. And the third was a expenimental group prescribed to compound 48/80 after medical treatment of Lithospermum erythrorhizon. Then, We investigated the experimental results by measuring the degree of degranulation and separation of histamine. Results: Lithospermum erythrorhizon did obviously inhibit the degree of degranulation of mast cell(p<0.05). Lithospermum erythrorhizon inhibited the separation of histamine in the plasma. Conclusion: Lithospermum erythrorhizon may be one of the effective therapeutic regimens on atopic dermatitis.

• Korean Journal of Medicinal Crop Science
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• v.21 no.1
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• pp.27-31
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• 2013

This study was conducted to isolate and identify the fungal pathogen causing seedling rot of Lithospermum erythrorhizon Siebold & Zuccarini, and to know the optimum growing temperature for decreasing seedling rot of Lithospermum erythrorhizon. On the basis of morphological characteristics, EF-1a sequence analysis, and pathogenecity to host plant, the fungi isolated from seedling rot and seeds of Lithospermum erythrorhizon were identified as Fusarium fujikuroi, indicating that disease causing fungus is seed-borne pathogen. Optimum temperature for germination of seeds of Lithospermum erythrorhizon was $15{\sim}20^{\circ}C$, but pathogenicity of Fusarium fujikuroi was shown more readily at $25{\sim}30^{\circ}C$. These results suggested that seedling culture of Lithospermum erythrorhizon between $15^{\circ}C$ and $20^{\circ}C$ might reduce seedling rot of Lithospermum erythrorhizon caused by seed-borne pathogen Fusarium fujikuroi.

• Journal of Fashion Business
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• v.18 no.3
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• pp.29-44
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• 2014

As there is a growing interest in natural ingredients, they are actively being studied to improve damaged skin However, the lip cosmetics are still being inadequately studied on the exposure of lips to several stimuli and easy flow of chemical on the lips into the body. This study was conducted to make a clinical evaluation on the real effect of lip balm on lips, by extracting oil from Chamaecyparis obtusa and Lithospermum erythrorhizon that are known to play a positive role on skin. The following three natural lip balms were produced: lip balm made with Chamaecyparis obtusa(LBWCO), lip balm made with both Chamaecyparis obtusa and Lithospermum erythrorhizon(LBWCOLE), lip balm made without Chamaecyparis obtusa or Lithospermum erythrorhizon(LBWICOLE). The quality of LBWCO or LBWCOLE was evaluated to be higher than that of LBWICOLE. LBWCO and LBWCOLE had the highest satisfaction and purchase intention, respectively. As stated above, Chamaecyparis obtusa and Lithospermum erythrorhizon could be the ingredients of lip balm, and they were effective in improving damaged lips. Consequently, this study will provide opportunities to explore excellent ingredients to be used in natural lip balm. Also, studying lip cosmetics using only the natural ingredients will provide opportunities to expand positive images of ingredients for producing lip cosmetics.

• Korean Journal of Pharmacognosy
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• v.40 no.4
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• pp.289-297
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• 2009

This study was carried out to investigate the ecological characteristic of native Lithospermum erythrorhizon population in Korea. The survey quadrates were located in the middle of native L. erythrorhizon habitats. As the result of the research, the ecological patterns of native habitats were found to be divided into inclined plane type, ridge type, mountain-roadside type, and rock bed type. The research revealed 90 taxa in total, in which 37 families, 63 genera, 23 varieties, 6 forma, 1 sub-species and 60 species were checked in the flora of native L. erythrorhizon habitats.

• Korean journal of food and cookery science
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• v.21 no.3 s.87
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• pp.283-289
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• 2005

To investigate the oxidative stability of the Lithospermum erythrorhizon extracted oil, we prepared extracted oil from the cultivated and wild roots of Lithospermum erythrorhizon by autoclave method with soybean oil. The oil were stored for 30 days at $60^{\circ}C$, and the peroxide value (POV), acid value (AV) and carbonyl value(CV) were measured periodically. The weight was highly decreased in the oil added roots of Lithospermum erythrorhizon during the storage period. POV of soybean oil containing wild and cultivated Lithospermum erythrorhizon was generally enhanced with prolonged storage time, with the POV of the samples being lower than 100 meq/kg.oil after 30 days of storage. However, the POV of soybean oil was higher than 100meq/kg.oil after 10 days of storage. The pattern of the changes of AV and CV of soybean oil containing wild and cultivated Lithospermum erythrorhizon, were almost constant during the experimental periods. Nevertheless, the pattern of the changes of AV of soybean oil was rapidly increased during 20 days of storage, and that of CV of soybean oil was rapidly increased duringdays of storage and then slowly increased during the remainder of the experimental period. However, soybein oil was rapidly increased during 20 days of storage and then slowly decreased during the reminder of the experimental period. The overall results suggest that wild and cultivated Lithosyermum erythrorhizon added antioxidant activities to the autooxidation of soybean oil.

• Microbiology and Biotechnology Letters
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• v.18 no.5
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• pp.525-529
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• 1990

Plant cell cultures of Lithospermum erythrorhizon were performed in stirred tank and packed-bed reactors with in situ extraction by n-hexadecane. The specific shikonin production and volumetric shikonin productivity of stirred tank reactor reached 1.5 mg shikoninlg cell and 400$\mu g$ shikonin/(L.day), respectively. In packed-bed reactor with calcium alginate-immobilized cells specific shikonin production and volumetric productivity reached 2.0 mg shikoninlg cell and 2857$\mu g$ shikonin/(L.day), which were 1.3 and 7.1 times higher than those of stirred tank reactor, respectively. The higher shikonin production and productivity of packed-bed reactor seemed to be due to high cell loading capacity of calcium alginate immobilized cells in packed-bed reactor and improved cell-cell contact.

• Archives of Pharmacal Research
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• v.22 no.4
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• pp.414-416
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• 1999

A shkionin derivative was isolated from the roots of Lithospermum erythrorhizon with silica gel column chromatography and preparative TLC. The structure of the isolated pigment was identified as propionylshikonin by NMR and mass spectrometric analysis. The isolation of propionylshiknin was for the first time in the nature.

• Applied Microscopy
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• v.40 no.1
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• pp.29-35
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• 2010

The present study was carried out to investigate the hepatoprotective effect of water extract of Lithospermum erythrorhizon on acute hepatotoxicity induced in Sprague-Dawley (SD) rats by a single dose of galactosamine (400 mg/kg, i.p). The animals were divided into four groups. The animals in the Con group were fed basal diet. GalN group were administered with galactosamine. LE200 and LE500 groups treated with water extract of Lithospermum erythrorhizon (such as 200 and 500 mg/kg/day, p.o) for 7 days before galactosamine injection. In the change of AST, ALT, ALP, GGT and LDH contents, as compared with GalN group, LE200 group were significantly decreased. According to the electron microscopical observation, liver cells were increased the lipid droplet, change of mitochondria in the GalN compared with LE200. These results suggest that administration of water extract of Lithospermum erythrorhizon suppress or retard galactosamine induced acute liver injury.