• The Plant Pathology Journal
• /
• 제24권4호
• /
• pp.453-460
• /
• 2008

In the course of in vitro and in vivo screening for bioherbicidal agents, a hyphomycete fungus, Myrothecium sp. F0252 was selected as a candidate for the biocontrol of weeds. The isolate was identified as Myrothecium roridum Tode ex. Fries based on the morphological characteristics and 18S ribosomal DNA sequence analysis and registered as Myrothecium roridum F0252. In order to evaluate the in vitro effect of M. roridum F0252 on germination of ladino clover and white clover (Trifolium repens L.) seeds, spore solution of the fungus was employed in two concentrations, $6.5{\times}10^6$ and $2.5{\times}10^7$ spores per mL and then inoculated to the seeds. The fungal spores inhibited the seed germination, infected the seedlings, and caused an abnormal withering and inhibition of seedling growth. In addition, when the herbicidal activity of crude ethyl acetate extract from the liquid culture was assessed on a mini-plant, duck-weed (Lemna paucicostata (L.) Hegelm.), the extract showed high inhibitory effect at the level of $12.5{\mu}g$ per mL. On the other hand, in vivo herbicidal activity of M. roridum F0252 was evaluated by a whole plant spray method. M. roridum F0252 exhibited strong and broad-spectrum herbicidal activity. The herbicidal values ranged from 95-100% against 7 weeds, including Abutilon avicennae and Xanthium strumarium, and 70-80% against Digitaria sanguinalis and Sagittaria pygmaea. When the nutritional utilization (95 carbon sources) pattern of M. roridum F0252 was investigated, it varied with water activity ($a_w$) and temperature conditions, supplying good, basic information in regard to nutritional utilization for proper cultivation and formulation. Our results showed that M. roridum F0252 might be used as a potential biocontrol agent against weedy plants.

• Journal of Microbiology and Biotechnology
• /
• 제25권9호
• /
• pp.1401-1409
• /
• 2015

The aim of this study was to develop a SYBR Green-based real-time PCR assay for the rapid, specific, and sensitive detection of Acidovorax avenae subsp. citrulli, which causes bacterial fruit blotch (BFB), a serious disease of cucurbit plants. The molecular and serological methods currently available for the detection of this pathogen are insufficiently sensitive and specific. Thus, a novel SYBR Green-based real-time PCR assay targeting the YD-repeat protein gene of A. avenae subsp. citrulli was developed. The specificity of the primer set was evaluated using DNA purified from 6 isolates of A. avenae subsp. citrulli, 7 other Acidovorax species, and 22 of non-targeted strains, including pathogens and non-pathogens. The AC158F/R primer set amplified a single band of the expected size from genomic DNA obtained from the A. avenae subsp. citrulli strains but not from the genomic DNA of other Acidovorax species, including that of other bacterial genera. Using this assay, it was possible to detect at least one genomeequivalents of the cloned amplified target DNA using 5 × 10⁰ fg/µl of purified genomic DNA per reaction or using a calibrated cell suspension, with 6.5 colony-forming units per reaction being employed. In addition, this assay is a highly sensitive and reliable method for identifying and quantifying the target pathogen in infected samples that does not require DNA extraction. Therefore, we suggest that this approach is suitable for the rapid and efficient diagnosis of A. avenae subsp. citrulli contaminations of seed lots and plants.

• Plant Resources
• /
• 제5권3호
• /
• pp.196-201
• /
• 2002

This study was conducted to investigate the relationships between yearly variations of climatic elements and yearly variations of productivity in sesame. In addition, correlation coefficients among yield and yield components were estimated. The data of yield and yield components were investigated for 10 years from 1992 to 2001. The meteorological data gathered at the Yeosu Weather Station for the same period were used to find out the relationships between climatic elements and productivity. Yearly variation of the amount of precipitation in July and September were large with coefficients of variation(c.v.) of 64.59, 92.47%, respectively, but the variation of the average temperature in June and August were relative small. Yield and plant height greatly with c. v. of 26.24, 23.41 %, respectively, 1, 000 grain weights show more or less c.v. of 3.83% and length capsule setting show still less variation. Correlation coefficients between maximun temperature in period of cultivation(from June to September) and yield are positively significant at the level of 5.1 %, respectively. Correlation coefficients amount the plant height, length capsule setting, number of capsules per plant, weight of 1, 000 grains and seed yield were positively significant at the level of 1 %, respectively. Simple linear regression equations by the least square method are estimated for number of capsules per plant(Y$_1$) and the maximun temperature in August(X) as $Y_1$=10.1255+0.1725X, and for yield(Y$_2$) and the maximun temperature in August(X) as $Y_2$=21.6151 + 1.3724X.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2017년도 9th Asian Crop Science Association conference
• /
• pp.336-336
• /
• 2017

High mortality and slow growth of seedling generally occurred in tray due to cold, foggy weather and severe seedling blight. Mechanical transplanting is hampered, farmers having economic loss. A sustainable method for raising mat type seedling is necessary. Experiments were conducted on disease control and seedling growth in dry seasons. Fungicides treated/untreated seeds were sown 120-130g in each tray containing pulverized fine grain loam/sandy loam soils. Seed covered with a thin layer of soil and irrigated. It was polythene covered (PC) for 72h and then every night along with 2/3 sprinkler irrigation (SI) per day. Untreated seedlings infected 53-93%. Pyraclostrobin, Azoxystrobin and Azoxystrobin+Difeconazole treated seeds/sprayed (72h) seedlings had no disease. Seedling height was 11.7-13.2 cm with compact and thick root mat at 25 days. Seeds treated at 0.2-0.3% (18-20h) showed no disease but severely in untreated seeds in all locations. Seedling height (13.8 cm), leaf age (3.7) and root number (8.5) were better when NPK were applied at 3-4-3 g/tray. Spraying of urea (1%) in addition with MOP, theovit and $ZnSo_4$ at 0.6-0.6-0.2% on 7 and $15^{th}$ days was also effective. PC showed significant growth (29.1-34.5%). Root mat was not good in flooded irrigation (FI). Disease was higher in SI (66.7-97.3%) than FI (0.7-3.7%) in untreated seeds regardless of polythene use. The protocol would be effective for raising mat type seedling in cold environment.

• 한국산림과학회지
• /
• 제84권3호
• /
• pp.356-360
• /
• 1995

잣나무림에 있어서 목재 및 종자 생산이 중요 경영 목적이므로 이러한 형질을 개량하기 위한 선발육종이 진행중에 있다. 본 연구는 잣나무의 유전모수 및 조기선발 효율 등을 추정하여 1세대 채종원을 전진세대 채종원으로 유도하는데 필요한 자료와 정보를 얻기 위하여 수행하였다. 1986년 50가계로 된 풍매 차대검정림을 3곳에 조성하여 6년간(5~10년생) 수고 생장을 조사하여 분석한 결과, LAR에 대한 수령간 유전 및 표현형 상관의 단순직선회귀는 유의차가 있었으며($r^2=0.7862$, 0.8256), 성숙기를 20년으로 가정하였을 때 조림 후 3년생이 1.96으로 조기선발 효율이 가장 높은 수령으로 추정되어 조기선발이 가능하였다.

• 한국재료학회지
• /
• 제22권8호
• /
• pp.433-438
• /
• 2012

A chemical process involves polymerization within microspheres, whereas a physical process involves the dispersion of polymer in a nonsolvent. Nano-sized monodisperse microspheres are usually prepared by chemical processes such as water-based emulsions, seed suspension polymerization, nonaqueous dispersion polymerization, and precipitation polymerizations. Polymerization was performed in a four-necked, separate-type flask equipped with a stirrer, a condenser, a nitrogen inlet, and a rubber stopper for adding the initiator with a syringe. Nitrogen was bubbled through the mixture of reagents for 1 hr. before elevating the temperature. Functional silane (3-mercaptopropyl)trimethoxysilane (MPTMS) was used for the modification of silica nanoparticles and the self-assembled monolayers obtained were characterized by X-ray photoelectron spectroscopy (XPS), laser scattering system (LSS), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), elemental analysis (EA), and thermogravimetric analysis (TGA). In addition, polymer microspheres were polymerized by radical polymerization of ${\gamma}$-mercaptopropyl modified silica nanoparticles (MPSN) and acrylamide monomer via precipitation polymerization; then, their characteristics were investigated. From the elemental analysis results, it can be concluded that the conversion rate of acrylamide monomer was 93% and that polyacrylamide grafted to MPSN nanospheres via the radical precipitation polymerization with AAm in ethanol solvent. The microspheres were successfully polymerized by the 'graft from' method.

• Bulletin of the Korean Chemical Society
• /
• 제34권4호
• /
• pp.1205-1211
• /
• 2013

The structural and optical properties of the ZnO, Al-doped ZnO, Ga-doped ZnO, and In-doped ZnO nanorods were investigated using field-emission scanning electron microscopy, X-ray diffraction, photoluminescence (PL) and ultraviolet-visible spectroscopy. All the nanorods grew with good alignment on the ZnO seed layers and the ZnO nanorod dimensions could be controlled by the addition of the various dopants. For instance, the diameter of the nanorods decreased with increasing atomic number of the dopants. The ratio between the near-band-edge emission (NBE) and the deep-level emission (DLE) intensities ($I_{NBE}/I_{DLE}$) obtained by PL gradually decreased because the DLE intensity from the nanorods gradually increased with increase in the atomic number of the dopants. We found that the dopants affected the structural and optical properties of the ZnO nanorods including their dimensions, lattice constants, residual stresses, bond lengths, PL properties, transmittance values, optical band gaps, and Urbach energies.

• 한국콘텐츠학회논문지
• /
• 제19권8호
• /
• pp.123-136
• /
• 2019

본 논문은 최근 다양한 HMR 제품에 대한 소비자의 요구가 증가함에 따라 국내 HMR 시장 세분화의 필요성을 인식하여, 탕 찌개류 3종 제품을 개발하고 시중에서 판매되고 있는 경쟁 제품들과 함께 소비자 기호도 분석을 통해 개발 제품에 대한 기초자료를 제공하고자 진행되었다. 사용된 시료는 탕(들깨탕), 찌개(된장, 짜글이)이며, 개발된 3종과 각 종류별로 경쟁제품군 2가지씩을 각각 선정하여 총 9개 제품을 30~50대 성인 남녀 60명을 대상으로 소비자 기호도를 조사하였다. 소비자 기호도는 외관, 색, 향, 맛, 전반적 기호도로 구성되었고, 강도는 점도, 단맛, 짠맛, 신맛, 감칠맛을 조사하였으며, 친숙함, 건강도, 구매의도, 추천의도도 함께 조사하였다. 소비자 기호도 조사 결과 개발제품들은 기존 제품들 사이에서 경쟁력이 있는 것으로 판단되며 보완이 필요한 비선호 요인을 파악하고 원인을 분석한 후 제품을 개선할 필요가 있을 것으로 생각된다.

• 대한건축학회논문집:구조계
• /
• 제34권2호
• /
• pp.75-82
• /
• 2018

Green roof in Korea has been recognized as part of landscape area and local governments enacted municipal ordinance for supporting green roof thereby assisting part of expenses spent for green roof within a range of budget for buildings which are eligible for the green roof support of existing buildings. However, it is necessary to enforce a regulation of mandatory green roof for new buildings in order to expand green roof application and provide a variety of assistances such as subsidy payment, tax relief, and mitigation of floor area ratio-related regulation as done in other advanced nations. In particular, LEED(Leadership in Environmental and Energy Design) in the USA applies additional point directly for green roof in terms of the reduction in heat island effects. For example, the following items are beneficial to have additional points: habitat preservation or restoration, external space, rainwater management, reduction in outdoor water usage, minimum energy performance, and optimum energy performance. In addition, the BREEAM(Building Research Establishment Environmental Assessment Method) in the UK specifies green roof as one of assessment items to be complied in terms of diversity of species. As such, LEED and BREEAM reflect direct and indirect effects of green roof on assessment criteria, which suggest a direction to green building certification criteria in Korea where only additional points are given for green roof according to soil depth.

• 한국환경복원기술학회지
• /
• 제24권4호
• /
• pp.1-14
• /
• 2021

This study suggested suitable soil textures that is proper to propagate native plants to manage and restore barren land in DMZ. Germination tests were conducted for 16 native herbaceous plants growing in the DMZ border area in accordance with FAO-BI (Biodiversity International) standards, and the germination rate and T₅₀ in vitro were investigated. In order to examine the germination characteristics according to the soil textures, we used gravel, bed and mixed soil and investigated the germination characteristics under ordinary room temperature conditions in the greenhouse. As a result, it was observed that the germination rate in the greenhouse was significantly decreased compared to the germination rate in vitro of the species advertised due to soil textures. T₅₀ between the in vitro and each soil texture showed significant differences whereas T₅₀ between soil textures alone did not in all species advertised. The germination rate in vitro of Aster koraiensis, Dendranthema zawadskii var. latilobum, Hosta clausa, and Hosta minor there was no significant difference compared to ordinary room temperature conditions. In addition, as the germination rate is demonstrated more than 70%, which is relatively higher than other species advertised, it is considered to have strong environmentally adaptable. On the other hand, considering that the 6 species of Leontopodium coreanum, Plantago major, Potentilla chinensis, Sedum kamtschaticum, Sedum latiovalifolium, and Veronica kiusiana demonstrated less than 50% of germination rate in vitro, it is expected to be difficult to propagate without pre-treatment. In order to use these 6 species as restoration material plants, it needs to be considered to pre-treat to improve germination rate, or to enhance the vitality of seeds by improving the seed gathering period and storage method.