• KIEAE Journal
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• v.10 no.3
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• pp.11-16
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• 2010

This paper has attempted to investigate the change in soil moisture volume and temperature of architecture by planting ground(soil thickness and soil mixture ratio) and ground cover plants(Sedum sarmentosum, Zoysia japonica, Chrysanthemum zawadskii) for middle region green roof system. For this, a test was conducted on the roof of Konkuk University building from April 2009 to October 2009. In terms of treatment, five types(SL, $P_7P_1L_2$, $P_6P_2L_2$, $P_5P_3L_2$, $P_4P_4L_2$) depending on soil mixture ratio and two types(15cm, 25cm) by soil depth were created. Results of soil moisture volume by soil mixture ratio in the 15cm soil thickness showed that the difference was significance between simple soil and mixture soil treatment, however, the statistical significance was not recognized according to soil mixture ratio. In case of 25cm soil thickness, soil moisture volume by soil mixture ratio was more higher 7Vol.%~10Vol.% in the mixture soil than simple soil treatment. In terms of districts planted ground cover plants, soil volume moisture differed among plants in the order Zoysia japonica 17.74 Vol.%$34.86^{\circ}C$, district non-planted $27.49^{\circ}C$, Sedum sarmentosum $25.11^{\circ}C$, Chrysanthemum zawadskii $23.08^{\circ}C$, Zoysia japonica $24.45^{\circ}C$ respectively So, concrete surface showed more higher $5^{\circ}C{\sim}15^{\circ}C$ than other things among the all the time. Result of inner temperature of the architecture and soil, it was measured inner of architecture $25.69^{\circ}C$, inner district non-planted $24.29^{\circ}C$, Chrysanthemum zawadskii $23.90^{\circ}C$, Zoysia japonica $24.02^{\circ}C$, Sedum sarmentosum $25.13^{\circ}C$, respectively.

• Journal of Plant Biotechnology
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• v.37 no.1
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• pp.84-88
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• 2010

To establish the system of In vitro plant regeneration, the floral bud and leaf explants of Sedum rotundifolium were cultured on the MS media supplemented with different concentration of 2,4-D, NAA, and BA. The callus induction was more effective in the floral explants than the leaf explants, and was the best on MS medium containing 1.0 or 2.0 mg/L 2,4-D and 1.0 mg/L BA. The highest numbers of shoots were regenerated when callus were cultured on MS medium containing 2.0 mg/L 2,4-D and 1.0 mg/L BA for 8 weeks. The normal root formation from shoot was effective on the MS medium containing IAA alone. The regenerated plantlets were transferred to the pot and acclimatized successfully.

• Journal of agriculture & life science
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• v.44 no.6
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• pp.61-67
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• 2010

Six evergreen ground-cover species including Ajuga reptans, Carex morrowii 'Variegata', Dianthus 'Night Star', Hedera helix 'Ivalace', Pachysandra terminalis, and Sedum rupestre, were tested for cold acclimation with three different light treatments (0, 30, and 70% of shading, respectively) to select potential candidates for over-wintering in central Korea. Among those species, Ajuga reptans, Carex morrowii 'Variegata', and Sedum rupestre performed well, which had more than 50% of survival rate in all light conditions. However, Dianthus 'Night Star' had less than 40% under any light treatment and the other two species, Hedera helix 'Ivalace' and Pachysandra terminalis, survived more than 40% only with 70% of shading condition. These results imply that those candidate species having less cold hardness in central Korea could have the potential to could over-winter as long as a proper light condition is provided.

• Korean Journal of Plant Taxonomy
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• v.34 no.4
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• pp.359-364
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• 2004

Sedum tosaense Makino (Crassulaceae), an unrecorded species in Korean flora, was collected from parasitic crater, Sangumburi in Jocheon-eup Bukjeju-gun, Jeju-do. This species is most similar to S. bulbiferum and S. orizifolium, but differs in some substantial characters. S. tosaense is perennial and stoloniferous herb with bulbils on stems, while S. bulbiferum is annual or biennial herb without bulbils in leaf axil. In addition, S. tosaense has leaves emarginate at apex whereas S. bulbiferum has leaves somewhat uneven at apex. the leaves of S. tosaense are conspicuously flattened and spatulate in shape at the stem without flowers, but those of S. orizifolium, are terete or slightly flattened and linear to narrowly oblong in shape.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.54 no.4
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• pp.552-560
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• 2021

This study investigated anti-inflammatory and antibacterial activities of Sedum takesimense ethanolic extract, and 3 isolated compounds. To confirm anti-inflammatory and anti-acne activities, a nitric oxide (NO) inhibition assay, pro-inflammatory factor (TNF-α, IL-1β, and IL-8) inhibition assays, and minimum inhibitory concentration (MIC) tests were performed. The 3 isolated compounds were identified as 4,6-di-O-galloylarbutin (OGA), 2,4,6-tri-O-galloyl-glucose (OGG), and 1,2,4,6-tetra-O-galloyl-β-glucose (TOGG). The ethanolic extract and isolated compounds (OGA, OGG, TOGG) effectively inhibited production of NO and pro-inflammatory cytokines (TNF-α, IL-1β, and IL-8). Furthermore, OGG and TOGG exhibited MIC values toward Cutibacterium acnes of 12.5 ㎍/mL and 3.2 ㎍/mL, respectively. These results suggest that S. takesimense extract exerts an anti-inflammatory effect on LPS-induced RAW264.7 cells, and an antibacterial efficacy against C. acnes.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.24 no.5
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• pp.15-23
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• 2021

This study was carried out to suggest an appropriate plant combination by evaluating the growth of flowering ground-cover plants planted with Festuca glauca 'Eljiah Blue' on the roof-top environment. As for the plant materials, Allium senescens and Chrysanthemum coreanum which are shorter than Festuca glauca 'Eljiah Blue' and Sedum takesimense and Agastache rugosa which are taller than Festuca glauca 'Eljiah Blue' were selected. Festuca glauca 'Eljiah Blue' was planted on Conrol, and Festuca glauca 'Eljiah Blue' with Allium senescens (T1), Festuca glauca 'Eljiah Blue' with Sedum takesimense (T2), Festuca glauca 'Eljiah Blue' with Agastache rugosa(T3), and Festuca glauca 'Eljiah Blue' with Chrysanthemum coreanum (T4) were planted in each experimental plot. Plant height and covering rate were measured to evaluate the growth of Festuca glauca 'Eljiah Blue'. Also, relative growth rate (RGR) of plant height, RGR of plant width, and mortality rate of the flowering ground-cover plants were estimated. Plant height and cover rate of Festuca glauca 'Eljiah Blue' was greatest in T3. RGR of plant height was greater in the order of Agastache rugosa, Allium senescens, Chrysanthemum coreanum, and Sedum takesimense. In particular, RGR of plant width was also greatest for Agastache rugosa. Mortality rates of Agastache rugosa and Allium senescens were lowest at 11%. Therefore, based on good growth of Festuca glauca 'Eljiah Blue' planted with Agastache rugosa, these results were suggested as a desirable combination of plant species for rooftop gardening.

• KIEAE Journal
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• v.12 no.5
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• pp.85-92
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• 2012

This research was executed to analyze the problems of Korean type of 'Light-weight' Greening that has been applied similarly by the type of 'Intensive Greening,' and to suggest the better way. To achieve this purpose, we compared and analyzed the theory about the definition of Korean type of Roof Greening and the type of Greening of FLL. And we researched documents and fields of 18 sites of Seoul City that applied the type of 'Light-weigh' Greening, and produced improvement issues and directions. To resolve the confusion of the type definition and site application, arising from considering 'weight of greening' as the main viewpoint to classify the type of Greening, we have to change the term 'Light-weight' with more suitable for contents-based definitions. According to a 'Light-weight' Greening field survey, only 5 among 18 sites are suitable for Extensive Greening and the rest sites show the character of Intensive Greening. Korean concept of 'Light-weight' Greening corresponds with the definition of 'Weight of Greening', but has a problem that does not correspond with the viewpoint of 'Maintenance of Greening'. This problem comes from the fact that the surveyed Light-weight(extensive) Greening sites are designed and executed for use. Therefore, Extensive Greening is proper to be applied for sites, excluded from use. 5 sites, determined suitable for Extensive Greening, adopts 'Sedum-herbaceous Planting' or 'Sedum-grasses Planting' forms of Greening, based on Sedum. So, it has to precede with selecting and breeding plants for developing various forms of Greening, suitable for Extensive Greening including 'Grasses-herbaceous Planting.'

• Korean Journal of Medicinal Crop Science
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• v.15 no.5
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• pp.357-361
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• 2007

This study was conducted to evaluate the vegetation structure of the Peucedanum japonicum community by the phytosociological method of floristic composition table and cluster analysis on the southern coast of Korea. The vegetation of the Peucedanum japonicum community was classified mainly into 2 communities such as the Miscanthus sinensis community and the Lysimachia mauritiana-Rosa wichuraiana community. The Carex boottiana and Sedum oryzifolium community were classified as the lower rank of Miscanthus sinensis community. On level 1 of the cluster analysis of plant species, they were classified into Lysimachia mauritiana and Rosa wichuraiana group, also Miscanthus sinensis, Carex boottiana and Sedum oryzifolium in Peucedanum japonicum community, which is similar to the community classification shown in the synoptic table. On level 1 of the cluster analysis of relev, inland coast with Jejudo was Lysimachia mauritiana and Rosa wichuraiana of group such as level 1 of the cluster analysis of plant species, and island coast with Geomundo was Miscanthus sinensis Carex boottiana and Sedum oryzifolium of group such as cluster analysis of plant species.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2002.11b
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• pp.30-30
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• 2002

꿩의비름 (Sedum erythrostichum)은 매우 우수한 지피식물이며 건조에 강한 대표적 식물로 바위정원 (rock garden)을 가꾸는데 있어서 중요한 수종으로 이용되며, 유럽등지에서는 지붕에 식재하기도 하며 최근에는 빌딩옥상녹화의 대표적 수종으로 식재되고 있다. 또한 한방에서는 경천이라 불리우기도 하는데 피부상처 치유 및 미백효과가 탁월하다고 알려져 있다. 본 연구에서는 Agrobacterium을 매개로한 꿩의비름의 형질전환 시스템을 개발하고 아울러 phosphinothricin-N-acetyltransferase (PAT) 유전자를 도입하여 제초제 저항 식물을 개발하고자 수행되었다. 꿩의비름 잎을 Agrobacterium에 담근후 0.5 mg/l NAA와 2 mg/1 BA가 첨가된 MS 배지에 3일간 공동배앙 하였다. 그 후 300 mg/1 cefotaxime이 첨가된 같은 배지에 옮겨 계대하면서 Agrobacterium을 제거하였다. 약 3주후에 잎 절편으로 부터 직접적으로 부정아가 형성되기 시작 하였는데 이 시기부터 잎 절편을 25 mg/1 kanamycin이 첨가된 선발배지에 옮겨 주었다. 이 결과 배양된 잎 절편 절편 중 3.75%에서 kanamycin에 저항하는 부정아를 얻을 수 있었다. 형질전환체는 X-gluc 반응, PCR, Southern, Nothern analysis를 통하여 확인하였다. 약 94%의 형질전환 식물체는 성공적으로 토양에 옮길 수 있었으며 약 3개월후에 꽃을 피웠다. 형질전환체는 제초제인 Basta ($^{(R)}$ phosphinothricine at 200 mg/1)를 살포하여 주었을 경우 생존함을 확인 하였다.

• Journal of Microbiology and Biotechnology
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• v.31 no.9
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• pp.1288-1294
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• 2021

There are a growing number of reports of hospital-acquired infections caused by pathogenic bacteria, especially methicillin-resistant Staphylococcus aureus (MRSA). Many plant products are now being used as a natural means of exploring antimicrobial agents against different types of human pathogenic bacteria. In this research, we sought to isolate and identify an active molecule from Sedum takesimense that has possible antibacterial activity against various clinical isolates of MRSA. NMR analysis revealed that the structure of the HPLC-purified compound was 1,2,4,6-tetra-O-galloyl-glucose. The minimum inhibitory concentration (MIC) of different extract fractions against numerous pathogenic bacteria was determined, and the actively purified compound has potent antibacterial activity against multidrug-resistant pathogenic bacteria, i.e., MRSA and its clinical isolates. In addition, the combination of the active compound and β-lactam antibiotics (e.g., oxacillin) demonstrated synergistic action against MRSA, with a fractional inhibitory concentration (FIC) index of 0.281. The current research revealed an alternative approach to combating pathogenesis caused by multi-drug resistant bacteria using plant materials. Furthermore, using a combination approach in which the active plant-derived compound is combined with antibiotics has proved to be a successful way of destroying pathogens synergistically.