• Title/Summary/Keyword: Secondary structure

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Development of Two-Step Temperature Process to Modulate the Physicochemical Properties of β-lactoglobulin Nanoparticles

  • Ha, Ho-Kyung;Nam, Gyeong-Won;Khang, Dongwoo;Park, Sung Jean;Lee, Mee-Ryung;Lee, Won-Jae
    • Food Science of Animal Resources
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    • v.37 no.1
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    • pp.123-133
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    • 2017
  • The development of a new manufacturing process, a two-step temperature treatment, to modulate the physicochemical properties of nanoparticles including the size is critical. This is because its physicochemical properties can be key factors affecting the cellular uptake and the bioavailability of bioactive compounds encapsulated in nanoparticles. The aims of this study were to produce (beta-lactoglobulin) ${\beta}-lg$ nanoparticles and to understand how two-step temperature treatment could affect the formation and physicochemical properties of ${\beta}-lg$ nanoparticles. The morphological and physicochemical properties of ${\beta}-lg$ nanoparticles were determined using atomic force microscopy and a particle size analyzer, respectively. Circular dichroism spectroscopy was used to investigate the secondary structure of ${\beta}-lg$. The surface hydrophobicity and free thiol groups of ${\beta}-lg$ were increased with a decrease in sub-ambient temperature and an increase in mild heat temperature. As sub-ambient temperature was decreased, a decrease in ${\alpha}-helical$ content and an increase in ${\beta}-sheet$ content were observed. The two-step temperature treatment firstly involved a sub-ambient temperature treatment from 5 to $20^{\circ}C$ for 30 min, followed secondly by a mild heat temperature treatment from 55 to $75^{\circ}C$ for 10 min. This resulted in the production of spherically-shaped particles with a size ranging from 61 to 214 nm. Two-way ANOVA exhibited the finding that both sub-ambient and mild heat temperature significantly (p<0.0001) affected the size of nanoparticles. Zeta-potential values of ${\beta}-lg$ nanoparticles were reduced with increasing mild heat temperature. In conclusion, two-step temperature treatment was shown to play an important role in the manufacturing process - both due to its inducement of the conformational changes of ${\beta}-lg$ during nanoparticle formation, and due to its modulation of the physicochemical properties of ${\beta}-lg$ nanoparticles.

Effects of Surface Microstructure on Microwave Dielectric Properties of ZrO2-NiO added Ba(Zn1/3Ta2/3)O3 Ceramics (ZrO2와 NiO가 첨가된 Ba(Zn1/3Ta2/3)O3에서 표면 미세조직이 고주파 유전특성에 미치는 영향)

  • Kang, Sung-Woo;Kim, Tae-Heui;Moon, Joo-Ho;Kim, Sung-Youl;Park, Jun-Young;Choi, Sun-Hee;Kim, Joo-Sun
    • Journal of the Korean Ceramic Society
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    • v.45 no.11
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    • pp.701-706
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    • 2008
  • High frequency dielectric ceramics have potential for applications in mobile and satellite communications systems at frequencies higher than 10GHz. The Ba$(Zn_{1/3}Ta_{2/3})O_3$ ceramics are known to have a high quality factor, a small temperature coefficient of the resonance frequency and a high dielectric constant. On the other hands, sintering at high temperature for extended time is required to obtain the ordered structure for high quality factor. In this study, the microwave dielectric properties of $ZrO_2$ and NiO-added Ba$(Zn_{1/3}Ta_{2/3})O_3$ ceramics prepared by solid-state reaction have been investigated. Adding $ZrO_2$ and NiO could effectively promote the densification even the case of decreasing the sintering time. At the surface of samples, secondary phase of Ba-Ta compounds was formed possibly due to the evaporation of ZnO, however, the interior of the samples remained as pure Ba$(Zn_{1/3}Ta_{2/3})O_3$. The samples sintered at $1600^{\circ}C$ for 2h exhibited 1:2 ordering of Zn and Ta cations. Excellent microwave dielectric properties of $Q{\cdot}f$(>96,000 GHz) and ${\varepsilon}_r$=30 has been obtained.

A Correlation Analysis among Regional Industrial Diversification, Job Creation and Regional Competitiveness (산업구조 다양성과 고용창출 및 지역경쟁력간 관계 분석)

  • Kim, Dae-Jung;Kyung, Jong-Soo;Jung, Sung-Hoon
    • Journal of the Economic Geographical Society of Korea
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    • v.17 no.4
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    • pp.786-800
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    • 2014
  • The aim of this article is to analyze the relationship between the change of the diversity of the industrial structure, and regional employmentability and the intensification of regional competitiveness, and consequently, is to present policy implications. As a result of the analysis of the whole industry such as the primary, secondary and tertiary industry in Chungnam Province, the existing strategic implementation on the employmentability and regional competitiveness throughout the policy on the existing specialized industries can be regarded as appropriate. However, under the condition that danger and stability in each industry are fluctuated and the internal and external circumstances are varied, the detailed analysis and rapid policy prescription on the primary and tertiary industry are required. Along with these results, policy implications are as follows; Firstly, in terms of effectiveness, regional policy are simultaneously conducted along with industrial, technological and regional development's policies while consider ing complex and comprehensive characteristics of the regional industry and rapid changing the global market conditions. Secondly, with respect to equity, regional policy is needed to implement by reducing the socio-economic gap across regions and mixing regional and sectoral policies along with regional and local uniqueness.

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Effect of Sputtering Powers on Mg and Ga Co-Doped ZnO Thin Films with Transparent Conducting Characteristics (RF 마그네트론 스퍼터를 이용하여 제작한 MGZO 박막의 구조적 및 전기적, 광학적 특성에 미치는 스퍼터링 전력의 영향)

  • Kim, In Young;Shin, Seung Wook;Kim, Min Sung;Yun, Jae Ho;Heo, Gi Seok;Jeong, Chae Hwan;Moon, Jong-Ha;Lee, Jeong Yong;Kim, Jin Hyoek
    • Korean Journal of Materials Research
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    • v.23 no.3
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    • pp.155-160
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    • 2013
  • ZnO thin films co-doped with Mg and Ga (MxGyZzO, x + y + z = 1, x = 0.05, y = 0.02 and z = 0.93) were prepared on glass substrates by RF magnetron sputtering with different sputtering powers ranging from 100W to 200W at a substrate temperature of $350^{\circ}C$. The effects of the sputtering power on the structural, morphological, electrical, and optical properties of MGZO thin films were investigated. The X-ray diffraction patterns showed that all the MGZO thin films were grown as a hexagonal wurtzite phase with the preferred orientation on the c-axis without secondary phases such as MgO, $Ga_2O_3$, or $ZnGa_2O_4$. The intensity of the diffraction peak from the (0002) plane of the MGZO thin films was enhanced as the sputtering power increased. The (0002) peak positions of the MGZO thin films was shifted toward, a high diffraction angle as the sputtering power increased. Cross-sectional field emission scanning electron microscopy images of the MGZO thin films showed that all of these films had a columnar structure and their thickness increased with an increase in the sputtering power. MGZO thin film deposited at the sputtering power of 200W showed the best electrical characteristics in terms of the carrier concentration ($4.71{\times}10^{20}cm^{-3}$), charge carrier mobility ($10.2cm^2V^{-1}s^{-1}$) and a minimum resistivity ($1.3{\times}10^{-3}{\Omega}cm$). A UV-visible spectroscopy assessment showed that the MGZO thin films had high transmittance of more than 80 % in the visible region and that the absorption edges of MGZO thin films were very sharp and shifted toward the higher wavelength side, from 270 nm to 340 nm, with an increase in the sputtering power. The band-gap energy of MGZO thin films was widened from 3.74 eV to 3.92 eV with the change in the sputtering power.

A Proposal for a New Industrial Classification System by Service Economy Perspective (서비스경제 관점의 산업분류체계 개선 제안)

  • Chae, Jongdae;Kim, Hyunsoo
    • Journal of Service Research and Studies
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    • v.8 no.1
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    • pp.89-102
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    • 2018
  • The Industrial Classification is a systematic taxonomy of industrial activities and the Standard Industrial Classification is used in all country by their own a consistent classification method. Therefore, it is employed to analyze current status of industry affairs using statistical investigations in terms industrial activities for making industrial policies and to compare industrial activity among countries. Since the Second Industrial Revolution, the need for the homogenous standard of industrial classification among countries emerged as the economic and industrial exchanges between the countries have became more active. In 1940, Colin Clark who british economist divided the industry into the first (primitive), second (processed), and third (service) industries. Based on this, the United Nations Office for Statistics (UNSD) established International Standard Industry Classification (ISIC) in 1948, which most countries invoke it. ISIC(International Standard Industry Classification) and the standard industry classifications of countries have reached the present after several revisions since the enactment of the Act. In the 2000s, the standard industry classification is amended to reflect the emergence of new industries and changes in industrial structure, mainly featuring the creation and segmentation of sections in the tertiary industry domains. It also shows that primary and secondary sectors are shifting to tertiary industry. In this study, the causes of these common phenomena are systematically identified and the problems present classification systems have been analyzed. Also proposed is the direction of formation of the industrial classification system from a service economy point of view and the conceptual model of the new classification system. In the future, it is necessary to validate the proposed model through this study and to carry out various new classification system studies.

A 3D ground penetrating radar imaging of the heavy rainfall-induced deformation around a river levee: a case study of Ara River, Saitama, Japan (폭우에 의해 발생된 강 제방 주변 변형의 3차원 GPR 영상화: 일본 사이타마현의 아라강에 대한 현장적용사례)

  • Yokota, Toshiyuki;Inazaki, Tomio;Shinagawa, Shunsuke;Ueda, Takumi
    • Geophysics and Geophysical Exploration
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    • v.12 no.1
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    • pp.49-55
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    • 2009
  • This paper describes a three-dimensional ground penetrating radar (GPR) survey carried out around a levee of the Ara River in Saitama, Japan, where deformation of the ground was observed after heavy rainfall associated with the typhoon of September 2007. The high-density 3D GPR survey was conducted as a series of closely adjacent four directional sets of 2D surveys at an area surrounding vertical cracks on the paved road caused by deformations induced by heavy rain. The survey directions of the 2D surveys were 0, 90, 45, and -45 degrees with respect to the paved road and the intervals between lines were less than 0.5 m. The 3D subsurface structure was accurately imaged by the result of data processing using Kirchhoff-type 3D migration. As a result, locations and vertical continuities of the heavy rainfall induced cracks in the paved road were clearly imaged. This will be a great help in considering the generation mechanisms of the cracks. Moreover, the current risk of a secondary disaster was found to be low, as no air-filled cavities were detected by the 3D GPR survey.

A Study on One Person Households in Korea (우리나라 단독가구의 실태에 관한 소고)

  • 배화옥
    • Korea journal of population studies
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    • v.16 no.2
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    • pp.125-139
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    • 1993
  • Korea has successfully achieved a lowered fertility level owing to the strong population control policy and effective family planning program. Along with fertility decline and decreased number of children in family, average number of household members has decreased and nontraditional households such as one person household and households composed of unrelated individuals have prolifirated, even though the absolute number of them are found minimal in Korea. However in recent years several data and survey results suggest that one person households are gradually in the increasing trend. The study aimed at investigating the real state of one person households in Korea and next analyzing the proportional distribution of one person households by a few socioeconomic characteristics, thus providing basic for eatablishing far-singhted population and social welfare policy in the future. Korea has experienced high growth rate of economy through government-led development plans starting from the 1960s. During the past three decades, Korea has shifted from the agricultural state to the industrialized one. In compliance with the economic growth, urbanization and industrialization have brought about rural-to-urban migration and a great bulk of young population migrated to urban areas, who are seeking for educational and job opportunities. Korean society has also been under drastic change in every aspect of life involving norms, tradition, and attitude, etc. Therefore, in spite of the prejudice on 'living alone' still remaining, young people gradually leave parents and home, and further form nontraditional households in urban areas. Current increase in the number of one person households is partly attributable to the increase in high female educational attainment and female participation in economic activities. As the industrial structure in Korea changes from primary into secondary and tertiary industries, job opportunities for service/sales and manufacturing are opened to young female labor force in the process of industrialization. Contrary to the formation of one person households by young people, the aged single households are composed when children in family leave one by one because of marriage, education, employment. In particular, a higher proportion of aged female single households occur in rural areas due to the mortality difference by sex. Based on the data released form the 1990 Population and Housing Census and National Fertility and Family Health Survey in 1985 and 1991, the study tried to examine the state of one person households in Korea. According to Census data, the number of one person households increased to 1, 021, 000 in 1990, comprising 9.0 percent of total households. And the survey reveal that among total 11, 540 households, 8.0 percent, 923 households, are composed of one person households. Generally, the proportion of female single households is greater than that of male ones, and a big proportion of one person households is concentrated in the 25-34 age bracket in urban areas and 65 years and more in rural areas. It is shown than one person householders in urban areas have higher educational attainment with 59.2 percent high schooling and over in 1991, Job seeking proved to be the main reason for leaving home and forming one person households. The number of young female single households with higher education and economic self-reliance are found nil and the study did not allow to analyze the causal realtionship between female education and employment and one person household formation. However more research and deep analysis on the causal facors on one person household formation using statistical method are believed to be necessary.

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Identification of Secretion Signals of Target Proteins in Salmonella enterica serovar Typhimurium and Construction of Secretion Vector using this Signal (Salmonella enterica serovar Typhimurium에서 Type III 분비장치의 표적단백질들의 분비신호 확인 및 Type III 분비장치를 이용한 Secretion Vector의 개발)

  • Choi, Hyuk-Jin;Eom, Joon-Ho;Cho, Jung-Ah;Lee, Sun;Lee, Kyoung-Mi;Lee, In-Soo;Park, Yong-Keun
    • Korean Journal of Microbiology
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    • v.36 no.4
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    • pp.254-258
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    • 2000
  • Invasion process of bacterial cell into intestinal epithelium is important in Salmonella infection. The invasion is induced by the proteins secreted by type III secretion appratus of Salmonella. It has been known that the proteins do not have N-terminal signal peptide existing in general secreted proteins. Recent studies on Yersinia reported that secretion signal of type III appratus may lie on 5'end secondary structure of mRNA of secreted protein. In this study, we constructed translational fusion of ompR and sopE, encoding type III secretion protein of Salmonella, and observed secretion of the fusion protein for investigating the secretion signal of Salmonella type III appratus. The sopE DNA fragments of the translational fusion contain the region of promoter and from start code to tenth or to fifth code. These translational fusions indicate that type III secretion signal of Salmonella is located on 5'end of mRNA encoding secreted protein. We constructed prototype of secretion vector using this signal to produce useful foreign protein.

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Degradation Characteristics of Carbon Dioxide Absorbents with Different Chemical Structures (상이한 화학적 구조를 가진 이산화탄소 흡수제의 열화특성)

  • Kim, Jun-Han;Lee, Ji-Hyun;Jang, Kyung-Ryong;Shim, Jae-Goo
    • Journal of Korean Society of Environmental Engineers
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    • v.31 no.10
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    • pp.883-892
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    • 2009
  • We evaluated the degradation properties of various alkanolamine absorbents (MEA, AMP, DEA, and MDEA) having different chemical structures for $CO_2$ capture. The degradation of $CO_2$ absorbent in general was known to be caused by oxygen which is in flue gas and by heat source, respectively. To analyze the effect of $CO_2$ and $O_2$ on degree of degradation, we conducted a variety of experiments at $30^{\circ}C$ and $60^{\circ}C$ (oxidative degradation) and $130^{\circ}C$ and $150^{\circ}C$ (thermal degradation), respectively. DEA showed the worst property for oxidative degradation in the presence of oxygen among the alkanolamine absorbents. In the case of thermal degradation, the degradation of absorbent was occurred for most of absorbents at $150^{\circ}C$. Among these absorbents, MEA and DEA gave the worst results. As a result, AMP which is a primary amine and having a steric hindrance showed the best result through the degradation test. But, the degradation of absorbent proceeded easily in the case of DEA which is a secondary amine and having 2 OH groups in terminal position. Consequently, we have evaluated the degree of degradation of various absorbents having different chemical structures to give the basic data for the development of alkanolamine absorbent.

A Review of Vegetation Succession in Warm-Temperate Evergreen Broad-Leaved Forests -Focusing on Actinodaphne lancifolia Community- (난온대 상록활엽수림 지역의 식생천이계열 고찰 -육박나무군락을 중심으로-)

  • Park, Seok-Gon;Choi, Song-Hyun;Lee, Sang-Cheol
    • Korean Journal of Environment and Ecology
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    • v.32 no.1
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    • pp.77-96
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    • 2018
  • We investigated and analyzed three Korean island sites (Bijin-do, Ae-do, and Bogil-do) and one Japanese site (Tachibanayama) of sword-leaf litsea (Actinodaphne lancifolia) forests, known as the climax forest, to discuss the vegetation succession sere of warm-temperature evergreen broad-leaved forests. We then reviewed the literature in Korea, Japan, China, and Taiwan to consider the distribution characteristics of evergreen broad-leaved forests, vegetation succession sere, and climax tree species. Although Mt. Tachibana and Ae-do showed the most advanced vegetation structure, the soil and ordination (CCA) analysis indicated that it was not enough to consider that the sword-leaf litsea forest was at the climax stage in the warm-temperature region. The Actinodaphne lancifolia forest is sparsely distributed in Korea and Japan while the common types of vegetation in the warm temperate zone region in East Asia are Machilus spp., Castanopsis spp., and Cyclobalanopsis spp. The vegetation succession sere of the Korean warm-temperature region is thought to have a secondary succession such as Pinus thunbergii, P. densiflora, Q. serrata (early stage) through Machilus thunbergii, innamomum yabunikkei, Neolitsea sericea, Actinodaphne lancifolia (middle stage) to Castanopsis sieboldii, Q. acuta, Q. salicina (climax stage). However, Machilus thunbergii will be the climax species as an edaphic climax in places where there is a strong influence of the sea wind, or it is difficult to supply the seeds of Castanopsis spp. and Cyclobalanopsis spp.