• Journal of Microbiology and Biotechnology
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• v.27 no.11
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• pp.1999-2009
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• 2017

The secretion efficiency of a protein in a Sec-type secretion system is mainly determined by an N-terminal signal peptide and its combination with its cognate protein. Five signal peptides, namely, two synthetic Sec-type and three Bacillus licheniformis alpha-amylase-derived signal peptides, were compared for periplasmic expression of the human growth hormone (hGH) in E. coli. Based on in silico predictions on the signal peptides' cleavage efficiencies and their corresponding mRNA secondary structures, a number of amino acid substitutions and silent mutations were considered in the modified signal sequences. The two synthetic signal peptides, specifically designed for hGH secretion in E. coli, differ in their N-terminal positively charged residues and hydrophobic region lengths. According to the mRNA secondary structure predictions, combinations of the protein and each of the five signal sequences could lead to different outcomes, especially when accessibility of the initiator ATG and ribosome binding sites were considered. In the experimental stage, the two synthetic signal peptides displayed complete processing and resulted in efficient secretion of the mature hGH in periplasmic regions, as was demonstrated by protein analysis. The three alpha-amylase-derived signal peptides, however, were processed partially from their precursors. Therefore, to achieve efficient secretion of a protein in a heterologous system, designing a specific signal peptide by using a combined approach of optimizations of the mRNA secondary structure and the signal peptide H-domain and cleavage site is recommended.

• Structural Engineering and Mechanics
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• v.43 no.3
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• pp.371-394
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• 2012

Accurate finite element (FE) models are needed in many applications of Civil Engineering such as health monitoring, damage detection, structural control, structural evaluation and assessment. Model accuracy depends on both the model structure (the form of the equations) and the model parameters (the coefficients of the equations), and can be generally improved through that process of experimental reconciliation known as model updating. However, modelling errors, including (i) errors in the model structure and (ii) errors in parameters excluded from adjustment, may bias the solution, leading to an updated model which replicates measurements but lacks physical meaning. In this paper, an application of ambient-vibration-based model updating to a large-scale benchmark prototype of a building structure is reported in which both types of error are met. The error in the model structure, originating from unmodelled secondary structural elements unexpectedly working as resonant appendages, is faced through a reduction of the experimental modal model. The error in the model parameters, due to the inevitable constraints imposed on parameters to avoid ill-conditioning and under-determinacy, is faced through a multi-model parameterization approach consisting in the generation and solution of a multitude of models, each characterized by a different set of updating parameters. Results show that modelling errors may significantly impair updating even in the case of seemingly simple systems and that multi-model reasoning, supported by physical insight, may effectively improve the accuracy and robustness of calibration.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2004.11a
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• pp.250-261
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• 2004

A novel method for ab initio prediction of protein tertiary structures, PROFESY (PROFile Enumerating SYstem), is introduced. This method utilizes secondary structure prediction information and fragment assembly. The secondary structure prediction of proteins is performed with the PREDICT method which uses PSI-BLAST to generate profiles and a distance measure in the pattern space. In order to predict the tertiary structure of a protein sequence, we assemble fragments in the fragment library constructed as a byproduct of PREDICT. The tertiary structure is obtained by minimizing the potential energy using the conformational space annealing method which enables one to sample diverse low lying minima of the energy function. We apply PROFESY for prediction of some proteins with known structures, which shows good performances. We also participated in CASP5 and applied PROFESY to new fold targets for blind predictions. The results were quite promising, despite the fact that PROFESY was in its early stage of development. In particular, the PROFESY result is the best for the hardest target T0161.

• Bulletin of the Korean Chemical Society
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• v.17 no.8
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• pp.671-673
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• 1996

• Transactions of the Korean Society of Mechanical Engineers B
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• v.22 no.4
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• pp.417-427
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• 1998

The effect of secondary flow on the heat transfer of a turbulent wake generated by a flat plate was experimentally investigated. The secondary flow was induced in a curved duct in which the flat plate wake generator was installed. All three components of turbulent heat flux were measured in the plane containing the mean radius of curvature of the curved duct. The results showed that mean temperature profiles deviate from the similarity of the straight wake because of the cold fluid transported from the free-stream. The half-width of the mean temperature profile increased rapidly by upwash motion of the secondary flow. The changes to turbulence structure caused by the secondary flow show more pronounced effect on heat transport than on momentum transport. This is because the response to the variation of flow conditions is delayed in temperature field. Negative production of the turbulent heat flux is observed in the inner wake region. From the conditional averaging, it has been found that the negative production of the turbulent heat flux is generated due to a mixing process between the hot and low momentum eddies occupied in the inner wake region and the cold and high momentum eddies in the potential region.

• Journal of The Korean Society of Agricultural Engineers
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• v.65 no.6
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• pp.51-63
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• 2023

To improve the problem that the settlement curve of the consolidation theory of Terzaghi does not match well with the actual settlement curve, we included a secondary compression settlement and analyzed it by varying the beginning point and then obtained the following results. The current methods of calculating the compression index from the  log𝜎 curve and the coefficient of consolidation from the time-dependent settlement curve for each consolidation pressure proved that the final settlement amount will be consistent after a long time, but the actual settlement amount will always be smaller than the predicted settlement amount during the settlement progress stage. The consolidation factors estimated by the curve fitting with the condition that the secondary compression begins in the second half of the primary compression showed similar values to the consolidation factors estimated by the curve fitting for the primary compression only, and the settlement curves were in better agreement throughout the compression. It showed different values, showing low validity. It can be inferred that secondary compression acts from the point when a significant portion of the excess pore water pressure is dissipated, and the loading stress begins to have more influence on the skeletal structure of the soil. Analysis results show that secondary compression begins at the range of 91 % to 98 % on the average degree of primary consolidation.

• Korean Journal of Crystallography
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• v.16 no.2
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• pp.66-74
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• 2005

Marine natural products with various bioactivities are featured with similar structure to the common secondary metabolites and generally modified by halogenides, such as chloride, bromide, and iodide ions. Vanadium haloperoxidase is a key enzyme for the production of marine natural products and a metalloenzyme which requires a cofactor of vanadate. This review will cover isolation of vanadium haloperoxidase and the protein structures, as well as reaction mechanism of the metalloenzyme. Finally, reactivity of vanadium haloperoxidase and the biosynthesis of the secondary metabolites of indole, terpenoids, and acetogenins will be described.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.25 no.2
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• pp.143-149
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• 2011

In this paper, IIR structures are proposed to reduce the computation complexity of the secondary-pass estimation in active noise control(ANC) systems. However, there are stability problems of using IIR models to reduce the computation complexity in ANC systems. To overcome these problems, we propose a stabilizing procedure of recursive least mean squares (RLMS) algorithms for eatimating the parameters of IIR models of the secondary path transfer functions. The multichannel ANC systems are implemented by using the TMS320C6713 DSP board to test the performance of computation complexity and stability of the proposed methods. Comparing the IIR filters with the FIR filters, the IIR filters can reduce 50[%] of the computation and obtain similar noise reduction result.

• Journal of The Korean Association For Science Education
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• v.21 no.5
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• pp.944-967
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• 2001

The purpose of this study is to compare and analyze secondary school chemistry education in Korea and China in order to identify implications for the improvement of Korean chemistry education. The school systems, curricula, and teacher education related to secondary chemistry education of both countries were compared and analyzed. The 6-3-3-4 school system is used in both countries, and national school curricula are formulated by the Ministries of Education in both countries. The 1996 chemistry curricular standard for advanced middle schools in China, and 1997 chemistry curriculum for Korean high schools were compared in several aspects, followed by comparisons of chemistry teacher education in both countries. Based on the comparative analysis of chemistry education, some ideas and issues which provide implications for improving Korean high school chemistry education were identified. Chemistry teaching in the junior secondary schools, tentative implementation of curricula, required course work in chemistry, structure of curricula, oral assessments, probation of teachers and other issues are identified and discussed in this study.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2003.05a
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• pp.23-26
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• 2003

Recently, the thrust vector control using a secondary flow injection which is accomplished by injecting a secondary flow into the supersonic exhaust flow through hole in the wall of the propulsion nozzle has been attention in the applications of the rocket propulsion system. In the present study, 3-dimensional compressible, Navier-Stokes equation to understand the SITVC(Secondary Injection Thrust Vector Control) flow field. The computational results are validated with previous experimental data available. The computational results are visualized detailed structure of shock wave induced by secondary flow and deflected supersonic jets.