• Title/Summary/Keyword: Screen Concentration

Search Result 282, Processing Time 0.031 seconds

Amperometric Detection of DNA by Electroreducation of O2 in an Enzyme-Amplified Two-Component Assay

  • Yoon Chang-Jung;Kim Hyug-Han
    • Journal of the Korean Electrochemical Society
    • /
    • v.7 no.1
    • /
    • pp.44-48
    • /
    • 2004
  • The two-component type enzyme amplified amperometric DNA assay is described to use an ambient $O_2$ of the substrate of the DNA labeling enzyme. Although the assay detects DNA only at > 0.5M concentration, a concentration $\~10^6$ fold higher than the sandwich-type enzyme amplified amperometric DNA assay, it can be run with an always available substrate. The assay utilizes screen-printed carbon electrodes (SPEs) which were pre-coated by a co-electrodeposited film of an electron conducting redox hydrogel and a 37-base long single-stranded DNA sequence. The DNA in the electron conducting film hybridizes and captures, when present, the 37-base long detection-DNA, which is labeled with bilirubin oxidase (BOD), an enzyme catalyzing the four-electron reduction of $O_2$ to water. Because the redox hydrogel electrically connects the BOD reaction centers to the electrode, completion of the sandwich converts the film from non-electrocatalytic to electrocatalytic for the reduction of $O_2$ to water when the electrode is poised at 200 mV vs. Ag/hgCl. The advantage or the assay over the earlier reported sandwich type enzyme amplified amperometric DNA assay, in which the amplifying enzyme was horseradish peroxidase, is that it utilizes ambient $O_2$ instead of the less stable and naturally unavailable $H_2O_2$.

Evaluation of Commercially Available Passive Samplers and Development of New Passive Samplers Part 2 : Development of New Passive Samplers (공기중 유기용제 농도 측정에 있어서 수동식 시료채취기의 성능평가 및 한국산 수동식 시료채취기의 개발에 관한 연구 제 2 부 : 한국산 수동식 시료채취기의 개발)

  • Paik, Nam Won;Kong, Sang Hui;Park, Jeong Im;Lee, Young Hwan
    • Journal of Korean Society of Occupational and Environmental Hygiene
    • /
    • v.6 no.1
    • /
    • pp.97-108
    • /
    • 1996
  • A new type of passive samplers were designed and produced by authors. After evaluating the quality of activated carbon by measuring recovery rate of organic vapors and steadiness of sampling rate, activated carbon with 30 - 35 mesh produced by Company S in Korea was selected. In each passive sampler, an amount of 400 mg of the activated carbon was filled in 25-mm cassette and covered by fixed screen (or wire screen with 100 mesh). In addition to the fixed screen, a wind screen (or wire screen with 300 mesh) was also attached at outer face. The sampling rate of the new Korean passive samplers was estimated Conclusions obtained in the study are as follows. 1. Sampling rates of the newly developed Korean passive samplers were affected by sampling time. For n-hexane, sampling rates of 15- and 60-minute samples were 70.92 and 37.45 ml/min, respectively. Sampling rate of both 200- and 450-minute samples was 25.96 ml/min. It is concluded that, when passive samplers are used for measuring organic vapors, samples be collected longer than 60 minutes. 2. Sampling rate of the passive samplers was also affected by airborne concentration of organic vapors. Lower sampling rates were determined at level of 1/2 threshold limit values (TLVs) recommended by the American Conference of Governmental Industrial Hygienists (ACGIH). It is recommended that sampling rate of the passive samplers be obtained at site by measuring concentrations using both the NIOSH Method and passive samplers simultaneously. 3. When the passive samplers, which collected organic vapors, were exposed to clean air for five hours, there was no significant loss of organic vapors due to reverse diffusion. 4. When samples were stored at room temperature ($21.8{\pm}0.7^{\circ}C$) and refrigerator ($3.8{\pm}0.7^{\circ}C$), there was no significant difference in the accuracy of results. For trichloroethylene and n-hexane, accuracies were within 25 % at both temperatures until seven days. However, poor accuracy exceeding 25 % was indicated in toluene from the first day. It is recommended that samples be stored at freezing temperature below $0^{\circ}C$. 5. Sampling efficiency was significantly affected by direction of the passive samplers. Results of samplers facing wind and down, respectively, were compared. Lower amount of organic vapors were collected when the sampler was oriented down. It is recommended that, when air velocity is low in plants, the passive samplers be oriented to the wind. However, when air velocity is high, the passive samplers be oriented down.

  • PDF

Performance Evaluation of Vortex Screen for Treatment of Fine Particles in Storm Runoff (Vortex Screen장치를 이용한 강우유출수내 미세입자 처리특성 분석)

  • Lee, Jun-Ho;Jung, Yun-Hee;Bang, Ki-Woong
    • Journal of Korean Society of Environmental Engineers
    • /
    • v.31 no.4
    • /
    • pp.256-262
    • /
    • 2009
  • The use of hydrodynamic separator is becoming increasingly popular for suspended solids reduction in urban storm runoff. This study is a laboratory investigation of the use of Vortex Screen to reduce the solids concentration of synthesized storm runoff. The synthesized storm runoff was made with water and addition of particles; manhole sediment, road sediment, fly ash, and ployvinyl chloride powder. Vortex Screen was made of acryl resin with 250 mm of diameter and height of 700 mm. To determine the removal efficiency for various influent concentrations of suspended solids (SS) and chemical oxygen demand (COD), tests were performed with different operational conditions. The samples were taken simultaneously at the influent storage tank and effluent tank, and measured SS and COD concentrations. The ranges of surface loading rate were 110 to 1,550 $m^3/m^2$/day, and influent SS concentrations were varied from 141 to 1,986 mg/L. This paper was intended to evaluate the effect of inlet baffle and the ratio of underflow to overflow ($Q_U/Q_O$) on particle separation efficiency for various particle size using Vortex Screen. It was found that when increase of $Q_U/Q_O$ from 10% to 20%, SS removal efficiency was increased about 6%. The range of SS and COD removal efficiencies of road sediment particle size 125<$d_p$<300 ${\mu}m$ were 68.0~81.0%, 53.1~71.9%, respectively. Results showed that SS removal efficiency with inlet baffle improved by about 10~20% compared without inlet baffle.

Air Quality Impact Analysis of Point and Area Sources (점오염원과 면오염원의 대기환경영향 분석)

  • 김영성;손재익
    • Journal of Korean Society for Atmospheric Environment
    • /
    • v.9 no.2
    • /
    • pp.168-173
    • /
    • 1993
  • Air quality impacts of point and area sources were analyzed by using ISCST2 with wind speed and stability class combinations of SCREEN. Stack height was important in determining the impact of point sources. With the stack height reduced to 21m from 75m, the concentration in the vicinity increased several times in spite of decreasing the emission rate by half. When the emission rates were same, concentrations from an area source of 10m release height were slightly lower than those from a point source of 21m stack height at the plume centerline. Bur the area source resulted in larger area of high concentration. Concentration from the point source was high in neutral to slightly unstable conditions with strong winds in a short distance, and in stable conditions with weak winds in a long distance. Concentration from the area source decreased with distance from the source, and was high in stable conditions with weak winds.

  • PDF

Fabrication of Mixed and Patterned Type of Remote Phosphors by Screen Printing Process and their Optical Properties

  • Kim, Byung-Ho;Hwang, Jonghee;Lee, Young Jin;Kim, Jin-Ho;Jeon, Dae-Woo;Lee, Mi Jai
    • Journal of the Korean Ceramic Society
    • /
    • v.53 no.4
    • /
    • pp.381-385
    • /
    • 2016
  • Recently, white LEDs, especially, warm white LEDs have been intensively investigated due to outstanding optical properties, long term stability and low power consumption. In this study, mixed type and patterned type of remote phosphors were prepared by screen printing process employing green and red phosphor. Each type of remote phosphor exhibited distinctive photoluminescence spectrum. For example, the mixed type of remote phosphor exhibited unique spectrum, while the patterned type showed expectable spectrum depending on the concentration of phosphors. This indicates that a small amount of red phosphor dramatically reduced the green photoluminescence in the case of mixed-type remote phosphor, whereas the effect was negligible in the patterned-type remote phosphor. The possibility of undesirable chemical reaction was further investigated by using scanning electron microscopy and X-ray diffraction.

Estimation of the Efficiency of a Silt Screen using a Vessel-mounted ADCP

  • Jin, Jae-Youll;Park, Jin-Soon;Song, Won-Oh;Kim, Sung-En;Lee, Kwang-Soo;Yum, Ki-Dai;Oh, Jae-Kyung
    • Proceedings of the Korean Society of Coastal and Ocean Engineers Conference
    • /
    • 2003.08a
    • /
    • pp.353-358
    • /
    • 2003
  • As fur quantitative evaluation of the amount of sediments released into the ambient waters by various works fur coastal development, the instrument and method of the measurement of suspended sediment concentration (SSC) are critical for estimating the efficiency of a silt screen to reduce the spreading of sediment plumes generated by coastal works. (omitted)

  • PDF

Estimation of Estrogenic Activity by E-screen Assay and Stepwise Analysis of Endocrine Disruptors in Pilot Sewage Treatment Plant (파일럿 규모의 하수 처리 공정별 E-screen Assay에 의한 에스트로겐 활성과 내분비계 장애물질의 농도 평가)

  • Lee, Ji-Ho;Park, Jong-Yol;Ra, Jin-Sung;Duong, Cuong N.;Lee, Byoung-Cheun;Kim, Sang-Don
    • Journal of Korean Society of Environmental Engineers
    • /
    • v.28 no.7
    • /
    • pp.697-703
    • /
    • 2006
  • Endocrine disruptors were measured with GC/MS in effluents discharged from sewage treatment processes in pilot scale for the purpose of water reuse. From that analysis, we compared the removal rate of them by treatment processes. Nonylphenol was mainly detected in effluents and high concentration from 0.36 to 0.94 ${\mu}g/L$. $17{\beta}$-estradiol(E2) and $17{\alpha}$-ethynylestradiol(EE2) were detected as below the limit of detection in effluent. Endocrine disruptors were removed effectively in the range from 50 to 100% by treatment process. EC50 value($9.0{\times}10^{-3}$ M) of $17{\beta}$-estradiol(E2) by dose response curve of E-screen assay has higher than that of bisphenol A($2.736{\times}10^{-5}M$) and p-octylphenol($9.760{\times}10^{-6}$ M). These results showed that alkylphenols have lower relative estrogen potency than other estrogens such as $17{\beta}$-estradiol(E2). Calculated estrogenic activity(ng-EEQ/L) was 2 times higher than measured total estrogenic activity which estimated by E-screen assay. Moreover estrogenic activity of effluent by treatment process showed very low as below 1 ng-EEQ/L.

A Real-Time Spatial DSS for Security Camera Image Monitoring

  • Park, Young-Hwan;Lee, Ook
    • Proceedings of the Korean Operations and Management Science Society Conference
    • /
    • 1998.10a
    • /
    • pp.413-414
    • /
    • 1998
  • This paper presents a real-time Spatial Decision Support System(SDSS) for security camera image monitoring. Other SDSSs are not real-time systems, i.e., they show the images that are already transformed into data format such as virtual reality. In our system, the image is broadcasted in real-time since the purpose of the security camera needs to do it in real-time. With these real-time images, other systems do not add up anything more; the screen just shows the images from the camera. However in our system, we created a motion detection system so that the supervisor(Judge) of a sec.urity monitoring system does not have to pay attention to it constantly. In other words, we created a judge advising system for the supervisor of the security monitoring system. Most of small objects do not need the supervisor's attention since they could be birds, cats, dogs, etc. if they show up in the screen image. In this new system the system only report the unusual change to the supervisor by calculating the motion and size of objects in the screen. Thus the supervisor can be liberated from the 24-hour concentration duty; instead he/she can be only alerted when the real security threat such as a big moving object like an human intruder appears. Thus this system can be called a real-time Spatial DSS. The utility of this system is proved mathematically by using the concept of entropy. In other words, big objects like human intruders increase the entropy of the screen images significantly therefore the supervisor must be alerted. Thus by proving its utility of the system theoretically, we can claim that our new real-time SDSS is superior to others which do not use our technique.hnique.

  • PDF

Fluorescence Characteristics of Gd2O2S:Tb3+ Intensifying Screen According to Increase of Tube Voltage (관전압 증가에 따른 Gd2O2S:Tb3+ 증감지의 형광특성)

  • Je, Jaeyong
    • Journal of the Korean Society of Radiology
    • /
    • v.8 no.5
    • /
    • pp.261-264
    • /
    • 2014
  • In this study, $Gd_2O_2S:Tb^{3+}$ was used as the component of the intensifying screen, and this study aims at analysis of fluorescent feature depending on the increase of tube voltage using spectrometer. When the released fluorescence was measured according to the increase of tube voltage, blue, green and red was observed, among which, $^5D_4-^7F_5$ which is applicable to green, was strongest. In addition, when the fluorescent of 50 kVp and 120 kVp were compared, 50 kVp was proved to release only 9.56% fluorescence of 120 kVp. In case when tube voltage which is higher than 100 kVp is used for the X-ray using $Gd_2O_2S:Tb^{3+}$ intensifying screen, the amount and strength of the fluorescence reaching the film increase drastically, so attention is demanded to get images of proper concentration.

Influence of Precursor Solution Coating Parameters on Ferroelectric Properties of Pb(Zr0.7Ti0.3)O3 Thick Films (Pb(Zr0.7Ti0.3)O3 후막의 강유전 특성에 전구체 용액의 코팅요소가 미치는 영향)

  • Park, Sang-Man;Yun, Sang-Eun;Lee, Sung-Gap
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
    • /
    • v.19 no.12
    • /
    • pp.1092-1098
    • /
    • 2006
  • The influence of the concentration of precursor solution and the number of solution coatings on the densification of the $Pb(Zr_xTi_{1-x})O_3$ (PZT) thick films was studied. PZT powder and PZT precursor solution were prepared by3 sol-gel method and PZT thick films were fabricated by the screen-printing method on the alumina substrates. The composition of powder and precursor solution were PZT(70/30) and PZT(30/70), respectively. The PZT precursor solution was spin-coated on the PZT thick films. A concentration of a coating solution was 0.5 to 2.0 mol/L[M] and the number of coating was repeated from 0 to 6. The XRD patterns of all PZT thick films shelved typical perovskite polycrystalline structure. The porosity of the thick films was decreased with increasing the number of coatings and 6-time coated films with 1.5 M showed the dense microstructure and thickness of about $60{\mu}m$. The relative dielectric constant of the PZT thick film was increased with increasing the number of solution coatings and the thick films with 1.5 M, 6-time coated showed the 698. The remanent polarization the 1.5 M and 6-time coated PZT thick films was $38.3{\mu}C/cm^2$.