The effect of nitric oxide (NO) treatment on the quality of kiwifruit, cv. Hayward, was studied at room temperature after cold storage for one or three months at $0^{\circ}C$. Kiwifruits cold-stored for one month were treated with $200{\mu}L{\cdot}L^{-1}$ NO and subsequently transferred to room temperature to monitor quality changes over the course of their shelf life. Weight loss was high in fruits not treated with NO. Ethylene production was delayed for two days by NO treatment, and respiration rate was reduced to less half than that of the control. The kiwifruits stored for three months were treated with $N_2$ and 100, 200, or $500{\mu}L{\cdot}L^{-1}$ NO, or air alone. The highest weight loss was observed in kiwifruit treated with $100{\mu}L{\cdot}L^{-1}$ NO. While ethylene production was high in fruits treated with $100{\mu}L{\cdot}L^{-1}$ NO and without the treatment, it was relatively low in the kiwifruit treated with 200 and $500{\mu}L{\cdot}L^{-1}$ NO. Firmness was abruptly decreased in fruits not treated with NO, while the kiwifruit exposed to $200{\mu}L{\cdot}L^{-1}$ NO maintained the s ame level of f irmness for 9 days a t room t emp erature. In addition, growth o f Botrytis cinerea was inhibited by NO as compared with the air and $N_2$ treatments. Our findings indicate that NO can be used effectively for prolonging shelf life and maintaining fruit quality during distribution after cold storage. The optimum NO concentration for cold-stored kiwifruits was found to be $200{\mu}L{\cdot}L^{-1}$.
Molecular methods were employed to investigate microorganisms in a thermophilic continuous stirred tank reactor(CSTR) used for continuous $H_2$ production. The reactor was inoculated with heat-treated anaerobic sludge and fed with a glucose-based medium. Denaturing gradient gel electrophoresis showed dynamic changes of bacterial populations in the reactor during 43 days of operation. Gas composition was constant from approximately 14 days but population shift still occurred. Populations affiliated with Fervidobactrium gondwanens and Thermoanaerobacterium thermosaccharolyticum were dominant on 21 and 41 days, respectively. Keeping pH of the medium at 5.0 could suppress methanogenic activity that was detected during initial operation period. $CH_4$ and mcrA detected in the samples obtained from the reactor or inoculum suggested the heat treatment condition employed in this study is not enough to remove methanogens in the inoculum. PCR using primer sets specific to 4 main orders of methanogens suggested that major $H_2$-consuming methanogens in the CSTR belong to the order Methanobacteriales.
This study is to investigate structure relationship between factors influencing or mediating store loyalty in service encounter. The result of study suggests salesperson's voluntary service has an effect on store loyalty through interpersonal relationship and store satisfaction. On the other hand, nonvoluntary service doesn't statistically influence interpersonal relationship but influences store loyalty through store satisfaction. Interestingly this results shows that store loyalty is consisted of cognitive loyalty, affective loyalty, intentional loyalty, and behavioral loyalty and each loyalty have an affect in sequence each other. Managerial implications on research result is followed as first, as an evaluation method of salesperson, it is useful to evaluate salesperson's behavioral performance. second, to build store loyalty, it must make an effort for marketer to differentiate through store promotion in terms of customer's cognitive perspectives. It is meaningful for these results to apply to current retailing situations practically.
Cho, Hyun Kyung;Jung, Young Sang;Cho, Nam Chul;Lee, Hun
Journal of Conservation Science
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v.30
no.2
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pp.111-122
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2014
In Bongseon-ri site, the central type and the local type of the Baekje tombs co-exist together. Many swords with ring pommel which to regional leaders in Seocheon the Beekje government granted are excavated from these tombs. These represent that the regional leaders were controlled by the Baekje government gradually. Four swords with ring pommel and one simple sword are investigated metallurgically and we seek what swords have a function of weapon or what sword produce for grave goods. The simple sword is made by solid carburizing technology and quenching of heat treatment so it's possibly used as a weapon. In contrast, four swords with ring pommel has little chance of weapon because the blade of swords consists of microstructure that have low hardness and the back of swords don't have microstructure can absorb the shock. The most identical characteristic of the simple sword and swords with ring pommel is that they are possible to classify clearly into practical/non-practical tools. Additionally, according to non-metallic inclusion analysis result of these swords, w$\ddot{u}$stite and glass phase are found together. Thus, it is able to say that reduction method in low temperature is applied during smelting process.
This study was conducted to investigate the changes in growth performance, blood hormone levels and immunity of weaning pigs by the installation of warm curtain for keeping warmth in pigpen. A total of sixty-four piglets was weaned at 21 d of age with an average body weight of 5.8 kg. Each thirty-two piglets were allocated into the pens with (TRT) or without warm curtain (CONT). Daily gain and feed intake were increased by 17% and 9% in TRT from 2nd week to 4th week compared with CONT, respectively. The occurrence of diarrhea was decreased by 62% in TRT from initial to 2nd week compared with CONT. There were no significant differences in blood hormone levels and immunity of weaning pigs between treatments. In conclusion, the installation of warm curtain, which supported high temperature in pigpen, was considered to be effective in improving growth performance and reducing diarrhea occurrence of weaning pigs.
Kim, Sol;Hwang, Yun-Jeong;Kim, Hee-Eun;Lu, Ming;Kim, An-D-Re;Moon, Ji-Young;Kang, Ho-Sung;Park, Jang-Su
Journal of Life Science
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v.16
no.6
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pp.1052-1059
/
2006
The heat shock response is induced by environmental stress, pathophysiological state and non-stress conditions and wide spread from bacteria to human. Although translations of most proteins are stopped under a heat shock response, heat shock proteins (HSPs) are produced to protect cell from stress. When heat shock response is induced, conformation of HSF1 was changed from monomer to trimer and HSF1 specifically binds to DNA, which was called a heat shock element(HSE) within the promoter of the heat shock genes. Human HSF1(hHSFl) contains five cysteine(Cys) residues. A thiol group(R-SH) of Cys is a strong nucleophile, the most readily oxidized and nitrosylated in amino acid chain. This consideration suggests that Cys residues may regulate the change of conformation and the activity of hHSF1 through a redox-dependent thiol/disulfide exchange reaction. We want to construct role of five Cys residues of hHSF by redox reagents. According to two studies, Cys residues are related to trimer formation of hHSF1. In this study, we want to demonstrate the correlation between structural change and DNA-binding activity of HSF1 through forming disulfide bond and trimerization. In this results, we could deduce that DNA binding activity of DNA binding domain wasn't affected by redox for always expose outside to easily bind to DNA. DNA binding activity of wild-type HSF's DNA binding domain was affected by conformational change, as conformational structure change (trimerization) caused DNA binding domain.
Cha, Sangwon;Oh, Eunha;Oh, Selim;Han, Sang Beom;Im, Hosub
Journal of Environmental Health Sciences
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v.47
no.1
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pp.64-77
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2021
Objective: Biological monitoring of trace elements in human blood samples has become an important indicator of the health environment. The purpose of this study was to detect and evaluate multiple metal items in blood samples based on ICP-MS, to perform comparative evaluation with the existing analysis method, and to develop and verify a new method. Methods: 100 μL of whole blood from 80 healthy subjects was used to analyze ten metals (Sb, tAs, Cd, Pb, Mn, Hg, Mo, Ni, Se, Tl) using ICP-MS. Verification of the analysis method included calculation of linearity, accuracy, precision and detection limits. In addition, a comparative test with the conventional graphite furnace atomic absorption spectroscopy (GF-AAS) method was performed. In the case of Pb, Cd, and Hg in whole blood, cross-analysis between Pb, Cd, and Hg analysis methods was performed to confirm the difference between the existing method and the new method (ICP-MS). Results: The coefficient of determination (R2) was 0.999 or higher in seven items and 0.995 or higher in three items. The Pb result showed that Pearson's correlation coefficient was very high at 0.983, and the intraclass correlation coefficient was 0.966. The Cd result showed that Pearson's correlation coefficient was 0.917 between the existing method and the new analysis concentration value. Its intraclass correlation coefficient was 0.960, and there was no significant difference between the two groups. Hg had a low correlation at 0.687, and the intraclass correlation coefficient was 0.761, which was lower than that of Pb and Cd. The intra-day and inter-day accuracy of Pd and Cd were satisfactory, but Hg did not meet the criteria for both accuracy and precision when compared with the conventional analysis method. Conclusion: This study can be meaningful in that it proposes a more efficient and feasible analysis method by verifying a blood heavy metal concentration experiment using multiple simultaneous analyses. All samples were processed and analyzed using the new ICP-MS. It was confirmed that the agreement between the two methods was very high, with the agreement between the current and new methods being 0.769 to 0.998. This study proposes an efficient simultaneous methodology capable of analyzing multiple elements with small samples. In the future, studies of various applications and the reliability of ICP-MS analysis methods are required, and research on the verification of accurate, precise, and continuous analysis methods is required.
Purpose: To analyze the possible association between magnetic resonance imaging signal intensity of temporomandibular joint disk and posterior attachment, and the type and extent of disk displacement, disk configuration, effusion and clinical signs in patients with internal derangement. Materials and Methods: Magnetic resonance images of the 132 temporomandibular joints of 66 patients with temporomandibular joint displacement were analyzed. The clinical findings were obtained by retrospective review of the patients' records. The type and extent of disk displacement, disk configuration and effusion were evaluated on the proton density MR images. The signal intensity from the anterior band, posterior band and posterior attachment were measured on MR images. The associations between the type and extent of disk displacement, disk configuration, effusion and clinical signs and the MR signal intensity of disk and posterior attachment were statistically analyzed by student's t-test. Results: Of 132 joints, 87 (65.9%) showed anterior disk displacement with reduction (ADR) and 45 (34.1%) showed anterior disk displacement without reduction (ADnR). The signals from posterior attachments were lower in joints with ADnR than those of ADR (p<0.05). The results showed statistically significant (p<0.05) association between the type and extent of disk displacement and disk configuration, and decreased signal intensity of posterior attachment. There were no statistical associations between pain, noise and limited mouth opening, and signal intensity of disk and posterior attachment. Conclusions: The average signal from posterior attachment was lower in joints with ADnR than that of ADR. The type and extent of disk displacement and disk configuration appeared to be correlated with the signal intensity from posterior attachment.
Purpose: The purpose of the paper is to assess the greening of investment flows as well as the orientation of financial sources of effective environmental protection. Both India and Vietnam are considered to be two Asian countries that are radically affected by global climate change. Governments of the two countries have implimented numerous measures against environmental pollution through the banking and finance sector. Developing green banking operations in India and Vietnam is a new direction in the socio-economic development strategy coupled with effective environmental protection. Research design, data and methodology: The data was mainly based on Asia Development Bank Institution (ADBI) and Bank of India (BOI) from 2015 - 2018. This paper focused on comparing and evaluating the development of green banks between the two countries. Result: The banks' contribution to green growth and green economy is shown in two basic aspects: (i) the connection between organizations, and individuals, and management process for investment projects, including environmental risks; and (ii) the operation of banks has a direct impact on the environment, through the application of technology to pay documents and apply e-banking. Conclusion: Paper reflects, compares and evaluates green banking operations in India and Vietnam to provide new directions aiming to develop financial and economic system along with effective and efficient step toward climate change control. India's green banking operations, after a specific assessment, will be lessons for the Government of Vietnam during the process of socio-economic development and environmental protection.
Journal of the Korean Applied Science and Technology
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v.37
no.4
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pp.794-806
/
2020
This study was conducted in order to attain an in-depth understanding of and describe the Ego-Integrity Phenomenon of the elderly of age 65 and older. The data was collected from June 2020 to July 2020 as an in-depth interview consisting of semi-structured questions. The data was analyzed using the Colaizzi's method, one of the phenomenology methods. All interviews were recorded and transcribed for analysis. Through analysis, 309 significant statements were extracted, and 88 formulations of meaning, 53 themes, 16 theme clusters, and 5 categories were derived. The categories and the theme clusters are as such : 'reflecting on the past life with delight' ('fruitfulness over the lifetime', 'achieving through fulfilling responsibilities', 'achieved specially and gratefully'), 'Currently lonely and distressed due to the bitter old days' ('Physical health needs and psychosocial needs are not met', 'Wasn't able to make good choices for parents and children', 'Elderly life is not easy'), 'Satisfaction from enduring tough and difficult journeys in life' ('Compensated for hard work at workplace and home', 'Problem solved through endurance and overcoming', 'Living a healthy elderly life by escaping from the habits of the past'), 'Realizing the way of life as an elderly' ('Now is the best and most satisfying moment', 'Managing self in old age', 'Living passionately, though it may be late', 'Retiring from the forefront of life with an abundant heart'), 'Passing on beautifully' ('Desires toward the later years', 'Hope during the last moments', 'Appearance and emotions when death approaches'). It was observed that during the Ego-Integrity Phenomenon of the elderly, they have come to discern the meaning of life by reminiscing the regrets and difficulties of the past, and through this reminiscence, they have come to attain a positive and transcendental hope for the rest of their lives. The Ego-Integrity of the elderly appeared to be subjective, dynamic, and occurring simultaneously rather than continuously. It is recommended to develop a variety of nursing interventions while taking into consideration the age and the type of experience of the elderly.
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