• Journal of Mushroom
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• v.16 no.4
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• pp.267-271
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• 2018

This study was carried out to select the suitable for bag cultivation of Lentinula edodes. We investigated the optimal major materials and its mixing ratio in bag cultivation of L. edodes, 'Sanjo701ho' and 'Nongjingo'. The Suitable substrates for L. edodes bag cultivation were oak sawdust and douglas fir sawdust and rice bran mixed ratio 40:40:20(v/v). At the result, the period mycelial incubation shortened up to 13~18 days compared to the control. And Yield of commercial fruiting bodies was 17~19% higher than that in control. We expect that the cultivation period of L. edodes will be shortened, and the yield increased in the medium replaced half of oak sawdust by douglas fir sawdust.

• Journal of the Korean Wood Science and Technology
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• v.26 no.1
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• pp.19-28
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• 1998

For cultivation on sawdust-bed of oak-mushroom until present time, inoculation of spawn on sawdust bed has been performed by sawdust spawn. But, liquid spawn may have advantages for rapid mass production of spawn, and now, sawdust-cultivation by liquid spawn inoculation should be applied instead of sawdust spawn. Therefore, investigations were performed to evaluate the effect of sawdust-cultivation by liquid spawn inoculation. The results were as follows: 1. When 11 kinds of liquid media were applied, the oak-mushroom culture medium was the most excellent in growth. Most suitable temperature at PDA was $25^{\circ}C$, and $22.5\sim27.5^{\circ}C$ in range were optimal for liquid culture. In liquid culture, amount of mycelial growth increases rapidly up to 40 days of cultivation. Incubation at fermentor brought yield of 106mg dry mycelia per 40ml media after 17 days. 2. In 1l-spawn bottle, growth of mycelium by inoculation of 20ml-liquid spawns were faster than 6g-sawdust spawn in spread of mycelia. On 2kg-bag culture, inoculations of 10ml-, 20ml- and 30ml-liquid spawns were all slower than 20g-sawdust spawn in mycelial spread. So, amount increasement in ampunt of liquid spawn should be discussed. Yields of mushrooms until third sproutings of 2kg-bag culture were 580g in 30ml-liquid spawn inoculation, but 510g, 486g and 470g from 20g-sawdust spawn, 20ml-liquid spawn and 10ml-liquid spawn, respectively. Thus, 30ml-liquid spawn inoculation was highest in yield.

• Journal of the Korean Wood Science and Technology
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• v.38 no.2
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• pp.124-134
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• 2010

This study was performed to evaluate the efficiency of using softwood as the sawdust medium for Lentinula edodes cultivation, effect of nutrient on the mycelial growth, spawning, the mushroom yield, and quality. The nitrogen nutrition significantly enhanced the mycelial growth of L. edodes. The glutamic acid in the L. leptolepis and P. koraiensis, and asparagine in the P. densiflora were appeared to slight increase in the mycelial growth. The vegetable oil showed very effective on the mycelial growth in the P. koraiensis sawdust medium. Carbon/nitrogen ratio of all the test was reduced after mycelial growth. The mycelial growth was exclusively dependent on reduction of carbon. The mushroom yield (32.7%) of the P. densiflora sawdust medium (carbon source: 3% active carbon, nitrogen source: 0.4% asparagines) was the best in mushroom production of L. edodes, followed by the Q. variabilis sawdust (35.4%) of the control medium. The diameter of mushroom cap was obtained from the P. densiflora sawdust (carbon source: 3% sucrose, nitrogen source: 0.4% potassium nitrate) and P. koraiensis sawdust (carbon source: 3% sucrose, nitrogen source: 0.4% potassium nitrate), and the P. koraiensis sawdust (carbon source: 3% xylose, nitrogen source: 0.4% glutamic acid, supplement: 0.05% amino acid), with values 71.5 mm, 71.5 mm and 72.1 mm, respectively. In the polypropylene bag cultivation, the weight losses of the block medium gradually increased for 80 days in the dark (13.8~16.8%) and then became stable in the range of 20.7~25.8%.

• The Korean Journal of Mycology
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• v.38 no.1
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• pp.5-7
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• 2010

Auricularia auricula-judae is an edible mushroom, which is known as wood ear, free ear, black ear mushroom, and free jelly fish. Present experiments were conducted to determine the possibility of artificial cultivation with oak sawdust of A. auricula-judae. The duration of mycelial growth and days of pinhead formation on oak sawdust bag (1.4 kg) were 29~32 days and 13~17 days, respectively. The yield of fresh fruit-body was 275~350 g.

• Journal of Mushroom
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• v.14 no.2
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• pp.34-38
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• 2016

This study was performed to determine most appropriate mixed ratio of brewers dried grain to wheat bran as a nutrient supplement in the plastic vinyl bag cultivation of Grifola frondosa. In the examination of an appropriate nutrient source, the IV substrate formulation (80 : 10 : 10, oak sawdust : wheat bran : brewers dried grain) resulted in 71.6 days to harvest and the highest yield of 142.6 g/bag. On the other hand, the investigation of the optimal mixing ratio of beer waste to wheat bran showed that the III substrate formulation (80 : 5 : 15, oak sawdust : wheat bran : brewers dried grain) resulted in the least days to harvest (61.8 days), with the highest yield of 140.8 g/bag.

• Korean Journal of Plant Resources
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• v.16 no.3
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• pp.218-222
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• 2003

This study was carried out to find optimum condition through sterilization methods for cultivation of Neutaribeosut (Oyster mushroom). 1. Yield of additive culture of pine sawdust, cotton and rice hulls at bag­cultivation of Neutaribeosut. was similiar to additive culture of pine sawdust, cotton and pulpe as conventional methods. 2. Yield of Neutaribeosut at low temp. sterilization method as 50 to 6$0^{\circ}C$ and 7days treatment was higher about 18 to 19％ than that of high temp. as 121$^{\circ}C$ and 90 minutes treatment. 3. In case of farmer­cost, low than high temperature sterilization method appeared lowly about 12％. So this method will be of advantage to farmers in both yield and farmer­cost.

• The Korean Journal of Mycology
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• v.44 no.4
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• pp.300-306
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• 2016

Recently, the incidence of oak wilt disease has been increasing in Korea, resulting in an increasing number of dead trees. In this study, we performed sawdust bag cultivation of shiitake (Lentinula edodes) using oak wilt-diseased logs and measured the antioxidant activities of the resulting mushrooms. For sawdust media, 4 types of logs (healthy, infected, dead, and fumigated ones) were used. As inocula, three strains of L. edodes (NIFoS 764, Sanjo 701ho, and Sanmaru 2ho) were used. The productivity of L. edodes from dead logs was similar to that from healthy logs. Except for the Sanmaru 2ho strain, fumigated logs and infected logs yielded a lower productivity of L. edodes compared to healthy logs. In terms of antioxidant activity, L. edodes grown on sawdust from fumigated logs showed higher values than those from other logs.

• The Korean Journal of Mycology
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• v.39 no.3
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• pp.171-174
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• 2011

This study was carried out to elucidate suitable supplementary nutrition for Neolentinus lepideus cultivation. The main substrate for cultivation was douglas fir sawdust, and supplementary materials for experiment were corn meal, corn hull, corncob, beet pulp, and potato starch. The higher amount of total nitrogen source was showed in corn meal (0.45%) than any other nutritive materials. Incubation ratio and cultivation period showed the best 97% and the shortest 43 days on medium with corn meal substrate. The fruit body yield and biological efficiency on medium with 10% corn meal were highest 122 g/1 kg and 35%, respectively. Factor analyses concerning fruiting yield showed total nitrogen source (r = 0.95) and crude fat (r = 0.65). These results are recommended that N. lepideus cultivation is corn meal material as the source of the supplementary nutrition and total nitrogen source as the factor of fruiting body yield on the Douglas fir sawdust medium.

• Journal of Mushroom
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• v.9 no.3
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• pp.105-109
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• 2011

This study was carried out to select the suitable substrates for bag cultivation of Lentinula edodes. We investigated the optimal additive materials and its mixing ratio in bag cultivation of L. edodes, Sanjo 701 ho. The suitable substrates for L. edodes bag cultivation were oak sawdust as new material plus deffatted corn flour, and corn husk as an additive at the ratio 8:1:1(v/v), as the result of shorter mycelial growth, higher biological efficient, and a higher yield than any other substrates.

• The Korean Journal of Mycology
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• v.38 no.2
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• pp.199-201
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• 2010

Ganoderma neo-japonicum, which is also known as black lingshi mushroom and medicinal mushroom. Present experiments were conducted to determine the possibility of artificial culture with oak sawdust of G. neo-japonicum. The duration of mycelial growth and days of pinhead formation of oak sawdust bag (2.4 kg) were 28~35 days and 25~29 days, respectively. The yield of mushroom fresh fruitbody was 135~157 g.