• 한국약용작물학회지
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• 제11권2호
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• pp.148-154
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• 2003

홍삼(panax ginseng C.A. Meyer) 추출물 단독의 항산화 활성과 홍삼추출물에 삼백초(Saururus chinensis(Lour.) Baill.)와 복분자(Rubus coreanus Miq.) 추출물을 농도별로 보충 급여 하였을 때 나타나는 상승효과를 알아보기 위하여 Sprague Dawley rat에 이들 추출물을 8주간 섭취시킨 후 희생시켜 간장 cytosol중의 superoxide dismutase, catalase, glutathione peroxidase, GST 등의 항상화 효소계 활성을 측정하였다. 그 결과, 홍삼급여군의 superoxide dismutase, catalase, glutathione peroxidase의 효소활성이 대조군에 비해 농도 의존적으로 높았다. 특히 홍삼 단독보다는 삼백초와 복분자를 보충급여시 상승효과가 높았으며 급여기간이 길수록 그 효과가 높았다.

• Journal of Applied Biological Chemistry
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• 제63권3호
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• pp.197-201
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• 2020

In order to enhance the extraction efficiency of flavonoid from Saururus chinensis, carbohydrate-hydrolyzing enzyme Viscozyme L aided extraction techniques have been studied. Then flavonoid composition, as well as quercetin, were also identified using UV/Vis, HPLC/MS, and ¹H-NMR. The results showed that favorable extraction conditions were Viscozyme L concentration of 0.25 mg/g, pH 4.2, reaction at 45 ℃ for 12 h. Under the favorable extraction condition, total flavonoid yield (37.9 mg/g) and quercetin yield (0.86 mg/g) increased by about 2.0 and 9.6 times, respectively, compared to control group. Interestingly, as a significant flavonoid of S. chinensis, flavonoid glycones rutin was hydrolyzed to aglycones quercetin by Viscozyme L. These findings provide scientific and theoretical support for the development quercetin-rich products, which was quickly absorbed by the human body than rutin.

• 동의생리병리학회지
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• 제19권1호
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• pp.146-151
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• 2005

We investigated the effect of Saururus chinensis (Lour.) Baill (SCB) on allergy in mice. We conformed compound 48/80-induced mesenteric mast cell degranulation, active systemic anaphylatic shock and histamine release. Also observed acetic acid-induced vascular permeability and anti-dinitrophenyl (DNP) IgE-mediated passive cutaneous anaphylaxis. SCB inhibited mesenteric mast cell degranulation and active systemic anaphylatic shock induced by compound 48/80 dose-dependently. When SCB was pretreated by intra-peritoneal injection, the plasma histamine levels were reduced. SCB also significantly inhibited acetic acid-induced vascular permeability and anti-DNP IgE-mediated passive cutaneous anaphylaxis. In addition, SCB reduced IL-10 mRNA expression of the lung on ovalbumin-induced allergy. These results indicate that SCB inhibits allergy.

• Korean Journal of Acupuncture
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• 제21권3호
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• pp.97-104
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• 2004

Objective We investigated the effect of Saururus chinensis (Lour.) Baill. aquacupuncture (SCB) on anaphylaxis in mice. Methods : We conformed compound 48/80-induced mesenteric mast cell degranulation, active systemic anaphylatic shock and histamine release. Also observed acetic acid-induced vascular permeability and anti-dinitrophenyl (DNP) IgE-mediated passive cutaneous anaphylaxis. Results : SCB inhibited mesenteric mast cell degranulation and active systemic anaphylatic shock induced by compound 48/80 dose-dependently. When SCB was pretreated by intra-peritoneal injection, the serum histamine levels were reduced. SCB also significantly inhibited acetic acid-induced vascular permeability. In addition, SCB showed a significant inhibitory effect on anti-dinitrophenyl (DNP) IgE-mediated passive cutaneous anaphylaxis. Conclusion : These results indicated that SCB inhibits anaphylatic reaction.

• 한국약용작물학회지
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• 제24권6호
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• pp.458-463
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• 2016

Background: The objective of the present study was to investigate the effect of storage conditions on the growth of Saururus chinensis (Lour.) Baill. rootstock. Methods and Results: Rootstocks of S. chinensis were stored in either soil or vermiculite that had been treated with a control treatment, diluted wood vinegar (50 or 100-fold), DF-100 (50-fold), or 1-naphthylacetamide and at $5^{\circ}C$ or $15^{\circ}C$. After 8 weeks, the stored roots were planted in the field, and both plant height and leaf number were observed after transplantation. The greatest number of leaves ($5.60{\pm}0.80$) was produced by roots that had been stored in soil treated with 100 fold dilution of wood vinegar and at $5^{\circ}C$. Meanwhile the maximum plant height ($6.92{\pm}0.78cm$) at 30 d after transplanting was observed for rootstocks that had been stored in soil treated with the 100 fold dilution of wood vinegar and at $15^{\circ}C$, whereas the maximum plant height at 60 d after transplanting ($26.46{\pm}0.71cm$) was observed for rootstocks that had been stored in soil treated with the 100-fold dilution of wood vinegar and at $5^{\circ}C$. Therefore, the storage of rootstocks in soil treated with the 100-fold dilution of wood vinegar and at temperatures at or below $5^{\circ}C$ was most effective, and it can be used to prevent the decay of roots during the postharvest management of S. chinensis rootstocks. Conclusions: The results of the present study indicate that, among the parameters examined, the storage of roots in soil that had been treated with the 100-foil dilution of wood vinegar is the most effective method for improving the growth of S. chinensis.

• 생약학회지
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• 제48권1호
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• pp.63-69
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• 2017

Saururus chinensis (Lour.) Baill. (Saururaceae) has been used as a traditional medicine for the treatment of dysuria, leukorrhea, eczema, jaundice and ascites in Korea, China and Japan. Ethanol extract of S. chinensis was successively partitioned as methylene chloride, ethyl acetate, n-butanol and $H_2O$ soluble fractions. Among those fractions the ethyl acetate soluble fraction showed the most potent DPPH radical scavenging and superoxide quenching activities. To verify antioxidant activities of ethyl acetate fraction, we checked the activities of superoxide dismutase (SOD) and catalase, and intracellular ROS level and oxidative stress tolerance in Caenorhabditis elegans. Furthermore, to see if increased stress tolerance of worms by treating of ethyl acetate fraction was due to regulation of stress-response gene, we quantified SOD-3 expression using transgenic strain. Consequently, ethyl acetate fraction elevated SOD and catalase activities of C. elegans, and reduced intracellular ROS accumulation in a dose-dependent manner. Moreover, ethyl acetate fraction-treated CF1553 worms exhibited significantly higher SOD-3::GFP intensity.

• 한국식물병리학회지
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• 제14권2호
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• pp.184-185
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• 1998

Rhizoctonia stem rot of Saururui herba [Saururus chinensis (Lour) Baill] was observed during plantations from 1996 to 1997 in Chungbuk area. Infected plant showed damping-off and stem rot at soil line. The causal fungus of stem rot isolated from the infected plant was identified as Rhizoctonia solani according to the criteria based on the cultural and morphological characteristics. The stem rot of Saururi herba caused by Rhizoctonia solani was first described in Korea, and the name the“Rhizoctonia black stem rot”is proposed.

• 대한본초학회지
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• 제33권3호
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• pp.11-18
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• 2018

Objectives : Saururus chinensis and Houttuynia cordata (Saururaceae) are perennial herbs using for medicinal purposes in Korea. The objectives of this study are to compare anatomical key characters between two medicinal plants and to provide fundamental information for the identification of two herbal medicines by using anatomical features. Methods : Cross-sections of root, rhizome, stem, petiole, and leaf for each species were observed in this study. Materials were analyzed through dehydration, paraffin embedding and micro-sectioning, and double staining with Safranin O and Fast-Green FCF. Observations of permanent preparation were conducted using light microscope. Results : S. chinensis and H. cordata were distinguished with anatomical differentiations; Idioblasts with essential oil were scattered in the parenchyma cell of cortex, pith, and phloem of S. chinensis, on the other hand, in H. cordata, idioblasts were distributed ring-shaped in the cortex of the root. S. chinensis had two cycles of vascular bundles in the stem while H. cordata had one cycle. Hypodermis layer was conspicuous in a stem of H. cordata, crystals were observed the only parenchyma in a stem of S. chinensis, and epidermal oil cells were developed in the epidermis of H. cordata. S. chinensis had air cavity at the cortex and pith of the stem. The shape of cross-section was polygonal in the stem of S. chinensis and was circular in the stem of H. cordata. Conclusions : We investigated anatomical study of Korean S. chinensis and H. cordata. To identify two herbal medicines, we considered main anatomical features and provided identification key here.

• 한국작물학회지
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• 제47권3호
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• pp.157-160
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• 2002

삼백초의 근경 수확방법을 인력수확에서 생력기계화 수확기술로 개선코자 트랙터부착 굴취기 등 4종의 수확기기의 기계수확 효율을 비교, 검토하였던 바 그 결과를 요약하면 다음과 같다. 1. 총생력시간은 인력에 비하여 경운기 경운 60%, 경운기부착 굴취기 64%, 트랙터부착 굴취기 67%절감되었다. 2. 근경의 손실율은 인력수확 13.9%, 경운기 경운 11.7%, 경운기부착 굴취기 9.8%, 트랙터부착 굴취기 5.1%로 기계수확에서 낮았다. 3. 상품 근경수량은 인력수확 3,032 kg/10a에 비하여 경운기 경운은 248 kg 감소하였으나, 경운기부착 굴취기와 트랙터부착 굴취기는 56kg, 320kg각각 증가되었으며, 총 근경수량은 인력수확에 비하여 기계수확에서 3-10%증가되었는데, 트랙터부착 굴취기는 10%의 증가로 가장 높은 수량을 보였다. 4. 경제성은 인력수확에 비하여 기계수확에서 13-27%높았으며, 트랙터 부착 굴취기는 27%로 가장 높았다. 5. 트랙터부착 굴취기는 생력효율, 상품수량, 총 근경수량 그리고 경제성에서 가장 높거나, 많은 것으로 나타나 적용가능성이 가장 높은 것으로 나타났다.

• 한국자원식물학회지
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• 제29권4호
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• pp.385-392
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• 2016

본 연구는 삼백초 종자의 체계적인 발아연구를 통한 대량의 실생번식법 확립 및 약용작물로써의 활용도를 높이기 위하여 수행되었다. 채종 후 정선이 완료된 종자는 즉시 휴면유형 분류를 위하여 배 관찰, 직파 발아율 및 불투수성 검정을 실시하였다. 이후 종자를 4 ± 1.0℃에 건조저장하면서 발아특성 연구에 사용하였다. 발아촉진을 유도하고자 생장조절제와 무기염류의 종류 및 농도를 달리한 용액에 24시간 침지처리 하였다. 연구의 결과, 저온처리 되지 않은 삼백초 종자는 분화가 불완전한 미숙배를 가지고 있었으며, 5% 미만의 낮은 발아율을 기록하였다. 또한 단순 침지처리에 의해 수분흡수가 원활하였으며, 형태적으로 미숙한 배가 신장 후 발아되었기 때문에 형태·생리적 휴면으로 생각되었다. 삼백초 종자는 생장조절제와 무기염류 처리에 의해 유의적인 발아촉진 효과가 확인되었으며, 특히 GA₃ 500 ㎎/L 처리구에서 발아세(GE)가 46.1%로 가장 높았다. 또한 KNO₃ 10 ~ 20 mM 처리구에서는 최종발아율(PG)이 54.3 ∼ 57.7% 수준으로 향상되었으므로, 균일한 육묘를 위한 생장조절제와 무기염류의 선택적 이용이 가능할 것으로 생각된다.