• Journal of Soil and Groundwater Environment
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• v.12 no.4
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• pp.86-93
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• 2007

In recent tests using tracer have been frequently conducted by fluorescent tracers. In this study, granular activated charcoal (GAC) as a detector for the fluorescent tracers (rhodamine WT and uranine) was investigated through laboratory and field tests. In the laboratory tests, tracer concentrations of rhodamine WT and uranine determined by the GAC were slightly different from those of standard solutions but they were excellent in linearity. Results show that GAC is excellent as tracer detector when concentration of the fluorescent tracers is greater than 10 & micro; g/L whileas no obvious differences in mixed solutions of the two tracers due to interferences. Compared to conventional methods of water sampling, field results shows a high potential of GAC as a tracer in the field. Our results also show that wet analysis is better for the lower concentrations of tracers whileas dry analysis is good for high concentrations of tracers. This study demonstrates that fluorescent tracer detection using the GAC is very useful and economical for a hydraulic connection between target areas and very longer period of the tracer test.

• Asian Journal of Turfgrass Science
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• v.22 no.1
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• pp.35-48
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• 2008

Seasonal and regional occurrence of oriental beetle, Blitopertha (=Exomala) orientalis was investigated using pheromone traps and soil sampling at three golf clubs in Busan, Gunpo, and Gimpo from 2004 to 2007. Adults of Blitopertha orientalis were found from early June to late August, but peak times were different depending on the location and year. Peak day of adult occurrence of B. orientalis was 25 June,2006 and 22 June 2007 in Busan, but 30 June,2006 and 29 June,2007 in Gunpo. Mean numbers of B. orientalis adults attracted to pheromone trap varied with locations. Larval development was faster in Busan than in Gunpo. The 2nd instars of B. orientalis was found on 27 August, 2004 in Gunpo whereas 3rd instars were found in Busan in the same period. In Busan, overwintered 3rd instars pupated from early May to mid-June and emerged from early June. Eggs laid at this time were hatched from late June Most 3rd instars were found at middle August and started to overwinter. The peak time of mating was 20:00 to 22:00 hour of the day.

• Korean Journal of Environmental Biology
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• v.33 no.3
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• pp.330-337
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• 2015

Pre-monsoon dynamics of zooplankton community were investigated in Yongwol West River tributary; wetland shaped Korean Peninsula. Zooplankters were collected at three sites (stn 1: Yongwol West River, stn 2: Pyungchang River, stn 3: Jucheon River) monthly during the period between March and July 2014 except April. Totally 58 taxa of zooplankton occurred, consisting of 27 species of rotifer, 18 species of cladoceran, eight species of copepod, four kinds of aquatic insect larvae and one nematod. Lotic cladocerans of Alona spp. belonging to Chydoridae occurred frequently while typical lentic ones of Bosmina spp. and Daphnia spp. were not distributed, then sampling sites may be located in lotic waters. Zooplankton abundance recorded as $4,451-8,011indiv{\cdot}m^{-3}$ in Jucheon River but never exceeded $500indiv{\cdot}m^{-3}$ in other rivers. In Yongwol West River, dominant taxa were aquatic insect larvae from March through June and succeeded to cladocerans and copepods in July. Similar succession was observed in Jucheon River where aquatic insect larvae dominated in March and changed to cladocerans and copepods from May with high abundance. No such succession of zooplankton community was detected in Pyunchang River. Species diversity indices were the highest in Yongwol West River except in March. High values of dissolved oxygen ($9.0-11.0 mg{\cdot}liter^{-1}$) may show the characteristics of mountain streams. Water temperature increased gradually from $10.1^{\circ}C$ to $27.9^{\circ}C$ and pH varied within generally high range between 7.9-8.9, respectively. High value of pH seems to be derived from lime stone mines and cement factories around the studied area.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.2
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• pp.341-353
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• 2000

The purpose of this study is to investigate the characteristics of pollutants overflow on storm events and the first flush analysis in industrial complex. Eight sub-basins in the Chongju industrial complex were selected for sampling and study with different characteristics during the period from June 1997 to August 1998. Water quality parameters of stormwater runoff such as $BOD_5$, COD, SS, TKN, $NO_3-N$, $PO_4-P$, TP, n-Hexane extracts, Cr, Cu, Pb and Fe were analyzed. During the storm events, measured concentration ranges of $BOD_5$, COD, SS, $NO_3-N$, TKN, $PO_4-P$, TP, r-Hexane extracts, Cr, Cu, Pb and Fe were 2~324 mg/L, 5~810 mg/L, 1~1,110 mg/L, 0.01~10.89 mg/L, 0.1~59.8 mg/L, 0.08~7.02 mg/L, 0.1~7.8 mg/L, 0.1~1,723.6 mg/L, 0.001~0.363 mg/L, 0.001~0.488 mg/L, 0.001~1.28 mg/L, and 0.26~18.91 mg/L, respectively in the industrial complex watersheds. And a quantitative of first flush has been presented. First flush occurs more frequently as the ratio of impervious area increases.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.2
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• pp.355-362
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• 2000

The objective of this paper is to estimate the pollutant load based on event mear concentrations(EMC) in industrial complex. Eight sub-basins in the Chongju industrial complex were selected for sampling and study with different characteristics during the period from June 1997 to August 1998. During the storm events, measured EMC ranges of $BOD_5$, COD, SS, TKN, TP, HEM, $NO_3-N$, $PO_4-P$, Cr, Pb, Cu and Fe in the industrial complex watersheds were 11~176mg/L, 40~502mg/L, 23~633mg/L, 104~20.9mg/L, 0.22~7.51mg/L, 12.7~548.7mg/L, 0.06~2.66mg/L, 0.12~3.39mg/L, 0.01~0.50mg/L. 0.02~0.42mg/L, 0.01~0.15mg/L and 1.29~11.51mg/L respectively. And the calculated annual average pollutant unit loadings of $BOD_5$ COD, SS, TKN, $NO_3-N$, $PO_4-P$, TP, HEM, Cr, Pb, Cu and Fe were 374.4kg/ha/yr 924.1kg/ha/yr, 983.6kg/ha/yr, 48.8kg/ha/yr, 8.1kg/ha/yr, 9.7kg/ha/yr, 17.8kg/ha/yr 943.0kg/ha/yr, 0.7kg/ha/yr, 0.9kg/ha/yr, 0.3kg/ha/yr and 28.9kg/ha/yr, respectively.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.11 no.2
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• pp.118-125
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• 2001

Recently, biochemical analysis using hemoglobin adduct is frequently performed to evaluate the exposure to chemical carcinogens. However, data on the effect of co-exposure with other chemicals on hemoglobin adduct formation are seldom provided. The objective of this study is to evaluate the effects of pretreatment of ethanol(EtOH) and phenobarbital(PB), which are known to affect metabolism of xenobiotics, on the formation of hemoglobin adducts in the rats(Sprague-Dawley) administered benzidine(BZ). The experimental rats were divided into control, EtOH, and P8 groups. Rats were pretreated with EtOH or PB 24 hours before the oral administration of BZ. Blood sampling was taken before the administration of the chemicals and 0.5, 3, 6, 9, 12, 24, 48, 72, 96, and 144 hours after the administration of the BZ in 5 rats each. The blood was separated into hemoglobin and plasma immediately after taking the blood samples, and the adducts were undergone basic hydrolysis to convert them into aromatic amines. Hydrolyzed BZ, monoacetylbenzidine (MABZ), and 4-aminobiphenyl(4ABP) were separated by reversed-phase liquid chromatography without derivatization, and quantitative analyses of them were performed by a highperformance liquid chromatograph equipped with electrochemical detector. The quantitative amount of the metabolites was expressed by hemoglobin binding index(HBI), BZ-, MABZ-, and 4ABP-HBI of EtOH and PB groups were increased more than those of control group. These results are attributable to the fact that EtOH and PB induced N-hydroxylation related to the hemoglobin adduct formation. The ratio of N-acetylation (viz, MABZ-HBI/BZ-HBI) showed no significant difference between EtOH group and control group. It means that EtOH increased N-hydroxylation and N-acetylation in a similar degree. The N-acetylation ratio of PB group was relatively lower than control group because the PB increased N-hydroxylation induction. The N-acetylation ratios of all groups were higher than 1 during the entire experimental period. This result suggests that the effects of EtOH or PB need to be considered in the biochemical monitoring for the assessment of intermittent exposure of benzidine.

• Journal of Environmental Science International
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• v.23 no.3
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• pp.433-445
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• 2014

The objective of study was to analyze seasonal and inter-annual patterns of water chemistry of Miho Stream watershed during 2004 - 2007 along with some influences of tributaries and summer monsoon on the stream water quality. For the study, eight physico-chemical parameters such as nitrogen, phosphorus, BOD, COD and chlorophyll-a (CHL) etc. were analyzed in relation to spatial and temporal variability of seven sampling sites of the mainstream and some tributaries in the watershed. In the upstream reach, Mean of BOD, COD and TP averaged 3.2 mg/L, 6.5 mg/L and $186{\mu}g/L$, respectively, indicating an eutrophic conditions as a III-rank in the stream water quality criteria from the Ministry of Environment, Korea(MEK). The eutrophic water was due to a combined effect of Chiljang tributary with high nutrients ($TP=844{\mu}g/L$, TN=8.087 mg/L) and the point sources from some wastewater treatment plants. In the meantime, BOD, TN, and TP in the downstream reach were about > 1.2-1.5 folds than the values of the midstream reaches. This was mainly attributed to effluents of nutrient rich-water (mean TN: 11.980 mg/L) from two tributaries of Musim Stream and Suknam Stream, which is directly influenced by nearby wastewater disposal plants. Seasonal analysis of water chemistry showed that summer monsoon rainfall was one of the important factors influencing the water quality, and water quality had a large spatial heterogeneity during the rainfall period. In the premonsoon, BOD in the downstream averaged $6.0{\pm}2.47mg/L$, which was 1.4-fold greater than the mean of upstream reach. Mean of CHL-a as an indicator of primary productivity in the water body, was > 2.2 - 2.9 fold in the downstream than in the upstream, and this was a result of the high phosphorus loading from the watershed. Overall, our data suggest that some nutrient controls in point-source tributary streams are required for efficient water quality management of Miho Stream.

• Korean Journal of Environmental Agriculture
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• v.32 no.4
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• pp.304-314
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• 2013

BACKGROUND: Ecological functions of irrigation ponds were well known fact that important for biodiversity conservation in agricultural ecosystems. However, many irrigation ponds were destructed with changes of agricultural environment. The objective of this study is to appreciate the importance of ecological functions of irrigation pond. Furthermore, it presented to useful information for restorations of irrigation pond from analyses of correlations between benthic macroinvertebrate communities and locational factors of irrigation ponds. METHODS AND RESULTS: Benthic macroinvertebrate sampling was conducted from 2010 to 2012 at 15 study ponds. Comparisons of benthic macroinvertebrates diversity approached species richness and density, and statistical analyses were performed using independent t-test. A total of 131 species / 137,118 individuals of benthic macroinvertebrates were recorded during study period. Dominant taxa of benthic macroinvertebrates included Coleoptera, Hemiptera, and Odonata. Generally, benthic macroinvertebrate diversity in mountain region and existing ponds were showed higher than open field and created ponds, respectively. DCA ordination showed that benthic macroinvertebrate community was most correlated with locational characteristics of irrigation pond, and it correlated with bank type and age of pond. CONCLUSION(S): In conclusions, in order to restore ecological irrigation pond, it is necessary to consider environmental factors such as locational characteristics and bank types.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.11
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• pp.1652-1662
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• 2014

The present study investigated the optimum blending condition of protected fat, choline and yeast culture for lowering of rumen temperature. The Box Benken experimental design, a fractional factorial arrangement, and response surface methodology were employed. The optimum blending condition was determined using the rumen simulated in vitro fermentation. An additive formulated on the optimum condition contained 50% of protected fat, 25% of yeast culture, 5% of choline, 7% of organic zinc, 6.5% of cinnamon, and 6.5% of stevioside. The feed additive was supplemented at a rate of 0.1% of diet (orchard grass:concentrate, 3:7) and compared with a control which had no additive. The treatment resulted in lower volatile fatty acid (VFA) concentration and biogas than the control. To investigate the effect of the optimized additive and feed energy levels on rumen and rectal temperatures, four rumen cannulated Hanwoo (Korean native beef breed) steers were in a $4{\times}4$ Latin square design. Energy levels were varied to low and high by altering the ratio of forage to concentrate in diet: low energy (6:4) and high energy (4:6). The additive was added at a rate of 0.1% of the diet. The following parameters were measured; feed intake, rumen and rectal temperatures, ruminal pH and VFA concentration. This study was conducted in an environmentally controlled house with temperature set at $30^{\circ}C$ and relative humidity levels of 70%. Steers were housed individually in raised crates to facilitate collection of urine and feces. The adaptation period was for 14 days, 2 days for sampling and 7 days for resting the animals. The additive significantly reduced both rumen (p<0.01) and rectal temperatures (p<0.001) without depressed feed intake. There were interactions (p<0.01) between energy level and additive on ruminal temperature. Neither additive nor energy level had an effect on total VFA concentration. The additive however, significantly increased (p<0.01) propionate and subsequently had lower acetate:propionate (A/P) ratios than non-additive supplementation. High concentrate diets had significantly lower pH. Interactions between energy and additive were observed (p<0.01) in ammonia nitrogen production. Supplementation of diets with the additive resulted in lower rumen and rectal temperatures, hence the additive showed promise in alleviating undesirable effects of heat stress in cattle.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.6
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• pp.888-898
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• 2018

Objective: The study was conducted to investigate the effects of replacing soybean meal (SBM) with different levels of detoxified Jatropha curcas kernel meal (DJM) in growing pig diets on growth performance, nutrients digestibility and meat edibility. Methods: A total of 144 pigs with initial body weight of $20.47{\pm}1.44kg$, were randomly allocated to 6 dietary treatments with 6 replications per treatment and 4 pigs per replication for a period of 79 days. Six diets (DJM0, DJM15, DJM30, DJM45, DJM60, and DJM75) were formulated using DJM to replace 0%, 15%, 30%, 45%, 60%, and 75% of SBM. From d 37 to 42, feces and urine were total collected from six barrows in each treatment. At day 79, thirty-six pigs were slaughtered for sampling. The feed intake and weight gain were recorded, while the intestinal morphology, digestive enzyme activities, nutrient digestibility and the content of residual phorbol esters in muscles were determined. Results: The results showed that increasing the replacement of SBM with DJM decreased the parameters including body weight, average daily gain, average daily feed intake, gain-to-feed ratio, weight and villus heights of duodenum, villus height and villus height/crypt depth of jejunum, digestive enzymes (protease, amylase, lipase, and trypsin) activities, and nutrients digestibility (nitrogen deposition, digestibility of nitrogen, energy digestibility, and total nitrogen utilization) (linear, p<0.05; quadratic, p<0.05) and there was no significant difference among DJM0, DJM15, and DJM30 in all measured indices. The highest diarrhea morbidity was observed in DJM75 (p<0.05). Phorbol esters were not detected in pig muscle tissues. Conclusion: The DJM was a good protein source for pigs, and could be used to replace SBM up to 30% (diet phorbol esters concentration at 5.5 mg/kg) in growing pig diets with no detrimental impacts on growth performance, nutrient utilization, and meat edibility.