• Journal of the Society of Cosmetic Scientists of Korea
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• v.43 no.2
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• pp.115-124
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• 2017

The objective of this study was to investigate skin whitening effect of Resorcinol dipentyl ether [1,3-di(pentyloxyl)benzene] by in vitro experiments. Resorcinol dipentyl ether was prepared by alkylation of resorcinol with 1-bromopentane. The reaction products were confirmed by NMR, MS and other analytical equipments. In order to evaluate the skin safety of resorcinol dipentyl ether, the cytotoxicity of the cells constituting the skin (keratinocyte, melanocyte, fibroblast) was analyzed and similar cell viability was observed in all cell lines as compared with the control group. Inhibition of extracellular melanin synthesis effect of resorcinol dipentyl ether was approximately 65.75% at $20{\mu}g/mL$ and inhibition of intracellular melanin synthesis effect of resorcinol dipentyl ether was approximately 53.89% at $20{\mu}g/mL$. The real-time PCR and western blot analysis of mRNA expression and protein expression of tyrosinase, TRP-1, and TRP-2 related to melanogenesis revealed that melanin inhibitory effect of resorcinol dipentyl ether was inhibited from the transcription stage respectively. Finally, this study suggested applicability of Resorcinol dipentyl ether [1,3-di(pentyloxyl)benzene] as a whitening functional cosmetic new material.

• Journal of Pharmaceutical Investigation
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• v.30 no.3
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• pp.191-199
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• 2000

Genomics is providing targets faster than we can validate them and combinatorial chemistry is providing new chemical entities faster than we can screen them. Historically, the drug discovery cascade has been established as a sequential process initiated with a potency screening against a selected biological target. In this sequential process, pharmacokinetics was often regarded as a low-throughput activity. Typically, limited pharmacokinetics studies would be conducted prior to acceptance of a compound for safety evaluation and, as a result, compounds often failed to reach a clinical testing due to unfavorable pharmacokinetic characteristics. A new paradigm in drug discovery has emerged in which the entire sample collection is rapidly screened using robotized high-throughput assays at the outset of the program. Higher-throughput pharmacokinetics (HTPK) is being achieved through introduction of new techniques, including automation for sample preparation and new experimental approaches. A number of in vitro and in vivo methods are being developed for the HTPK. In vitro studies, in which many cell lines are used to screen absorption and metabolism, are generally faster than in vivo screening, and, in this sense, in vitro screening is often considered as a real HTPK. Despite the elegance of the in vitro models, however, in vivo screenings are always essential for the final confirmation. Among these in vivo methods, cassette dosing technique, is believed the methods that is applicable in the screening of pharmacokinetics of many compounds at a time. The widespread use of liquid chromatography (LC) interfaced to mass spectrometry (MS) or tandem mass spectrometry (MS/MS) allowed the feasibility of the cassette dosing technique. Another approach to increase the throughput of in vivo screening of pharmacokinetics is to reduce the number of sample analysis. Two common approaches are used for this purpose. First, samples from identical study designs but that contain different drug candidate can be pooled to produce single set of samples, thus, reducing sample to be analyzed. Second, for a single test compound, serial plasma samples can be pooled to produce a single composite sample for analysis. In this review, we validated the issue whether the second method can be applied to practical screening of in vivo pharmacokinetics using data from seven of our previous bioequivalence studies. For a given drug, equally spaced serial plasma samples were pooled to achieve a 'Pooled Concentration' for the drug. An area under the plasma drug concentration-time curve (AUC) was then calculated theoretically using the pooled concentration and the predicted AUC value was statistically compared with the traditionally calculated AUC value. The comparison revealed that the sample pooling method generated reasonably accurate AUC values when compared with those obtained by the traditional approach. It is especially noteworthy that the accuracy was obtained by the analysis of only one sample instead of analyses of a number of samples that necessitates a significant man-power and time. Thus, we propose the sample pooling method as an alternative to in vivo pharmacokinetic approach in the selection potential lead(s) from combinatorial libraries.

• Journal of the Korean Geophysical Society
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• v.6 no.1
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• pp.39-46
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• 2003

Twin stone pagodas of the ruins of Kamunsa temple at Kyongju city, Kyungsangbukdo were believed to be built in 682 during the Unified Shilla Kingdom. The 13.4-m-high granodiolite pagodas with the base of 6.78 m x 4.4 m are the largest three-story stone pagoda in Korea. The western pagoda which was re-organized in 1959 is observed to be on the process of severe weathering. Also, some stone contacts are represented by the shape of sharp chevron, which is probably caused by the uneven loading due to the structural unbalance. For the structure-safety diagnosis of the western pagoda, it is necessary to understand its site characteristics and surrounding subsurface environment. Combined geophysical survey such as seismic and resistivity methods was carried out around the western pagoda. The range of 55∼350 Ωm is shown around the pagoda from the electrical resistivity mapping by the Wenner method. The higher resistivities occur the southwestern area, while the lower (<100 Ωm) values indicating the weaker subsurface appear to be on the northeastern area. This result coincides with the measurement of a leaning angle of the pagoda. Along 6 seismic lines, about 3-m-thick uppermost section around the pagoda shows the P-wave velocity of 200∼700 m/s from the refraction survey. Based on the integrated geophysical survey, the foundation of the pagoda is estimated to be in the form of 11-m-side square down to the depth of 3 m.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.15 no.2
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• pp.36-49
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• 2016

A subway system is one of the major transportation modes at a metropolitan area. When it meets the other lines, the metro station, so-called transferring station, is usually threatened by severe pedestrian congestion and safety issue of transit users including the transportation vulnerable. Although transportation planners forecast travel demand at the beginning, it is not easy to predict pedestrian flows precisely for a long term if land use plans have dramatically changed. Due to expensive costs, structural extension of metro stations is limited. Therefore, it requires efficient and technical improvements as meeting the demand of pedestrian and physical characteristics. In this study, the core mechanism of pedestrian movement-based simulation model was introduced and evacuation scenarios were analyzed with the developed model. As a result, the multiple optimal routes for unexpected events at the solid space of the multiple stories are easily searched through the simulator and in the case of Sadang Station, travel time can be reduced by 60% when the evacuation information and intuitive design are provided.

• Proceedings of the KSR Conference
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• 2008.11b
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• pp.30-36
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• 2008

The Spatial Image contents of Geomorphology 3-D environment is focused by the requirement and importance in the fields such as, national land development plan, telecommunication facility management, railway construction, general construction engineering, Ubiquitous city development, safety and disaster prevention engineering. The currently used DEM system using contour lines, which embodies geographic information based on the 2-D digital maps and facility information has limitation in implementation in reproducing the 3-D spatial city. Moreover, this method often neglects the altitude of the rail way infrastructure which has narrow width and long length. There it is needed to apply laser measurement technique in the spatial target object to obtain accuracy. Currently, the LiDAR data which combines the laser measurement skill and GPS has been introduced to obtain high resolution accuracy in the altitude measurement. In this paper, we tested of the railway facilities using laser surveying system, then we propose data a generation of spatial images for the optimal manage and synthesis of railway facility system in our 3-D spatial terrain information. For this object, LiDAR based height data transformed to DEM, and the realtime unification of the vector via digital image mapping and raster via exactness evaluation is transformed to make it possible to trace the model of generated 3-dimensional railway model with long distance for 3D tract model generation. As the results, We confirmed the solutions of varieties application for railway facilities management using 3-D spatial image contents.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2019.05a
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• pp.533-536
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• 2019

Recombinant baculoviruses are widely used to express heterologous genes in cultured insect cells. Recombinant baculoviruses can serve as gene-transfer vectors for expression of recombinant proteins in a wide range of mammalian cell types. Baculovirus system has significant benefits in view of safety, large-scale, and high level of gene expression. In this study, baculoviral vectors which were reconstructed from pOPINEneo-3C-GFP vector, were recombined with cytomegalovirus (CMV) promoter, green fluorescent protein (GFP), and p53 with NcoI and XhoI. These recombinant vectors were infected with various cells and cell lines. The baculovirus vector thus developed was analyzed by comparing the metastasis and expression of the recombinant genes with conventional vectors. These results suggest that the baculovirus vector has higher efficiency in metastasis and expression than the control vector. This work was supported by a grant from Mid-Career Researcher Program(NRF-2016R1A2B4016552) through the National Research Foundation of Korea(NRF) funded by the Ministry of Science, ICT & Future Planning(MSIP).

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.1
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• pp.777-785
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• 2021

Many utility lines are buried underground to provide various functions of the city. Because historical records are not managed systematically, damage has occurred during excavation. In addition, the demand for an underground facility management system is increasing as the aerial underground project is progressing. By attaching an electronic recognition sensor to an underground facility, such as pipelines, the management history and site conditions can be carefully managed. Therefore, in this study, electronic recognition sensors, such as BLE Beacon, UHF RFID, geomagnetic sensor, and commercial marker, were tested to analyze the strengths, weaknesses, and field applicability through a pilot project. According to the limited research results collected through two pilot projects, the installation depth is most important to demonstrate the performance of the electronic reader. In addition, because it should be used in urban areas, the influence of environmental interference should be minimized, and there should be no performance degradation over time. In the case of the geomagnetic recognizer, the effect of environmental interference was large, and performance degradation occurred over time using the BLE Beacon. In the field situation, where the installation depth can be controlled to less than 40cm, the utility of the battery-free UHF RFID was the best.

• Journal of the Korea Society of Computer and Information
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• v.27 no.3
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• pp.33-43
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• 2022

In this paper, we propose an ensemble model facilitated by multi-channel palm images with attention U-Net models and pretrained convolutional neural networks (CNNs) for establishing a contactless palm-based user identification system using conventional inexpensive camera sensors. Attention U-Net models are used to extract the areas of interest including hands (i.e., with fingers), palms (i.e., without fingers) and palm lines, which are combined to generate three channels being ped into the ensemble classifier. Then, the proposed palm information-based user identification system predicts the class using the classifier ensemble with three outperforming pre-trained CNN models. The proposed model demonstrates that the proposed model could achieve the classification accuracy, precision, recall, F1-score of 98.60%, 98.61%, 98.61%, 98.61% respectively, which indicate that the proposed model is effective even though we are using very cheap and inexpensive image sensors. We believe that in this COVID-19 pandemic circumstances, the proposed palm-based contactless user identification system can be an alternative, with high safety and reliability, compared with currently overwhelming contact-based systems.

• Journal of the Korea Institute of Military Science and Technology
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• v.24 no.6
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• pp.645-654
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• 2021

In this paper, we proposed a protocol to convert 2W telephone analog signals to Ethernet data in a private PSTN 2W tactical voice system. There are several kinds of operational problems in the tactical telephone network where 2W telephone copper lines are installed hundreds of meters away from the PBX in a headquarter site. The reason is that it is difficult to install and maintain the 2W telephone copper cable in severe operational fields and to meet safety and stability operational requirements of the telephone line under lighting and electromagnetic environments. In order to solve these challenging demands, we proposed an efficient method that the 2W analog interface signals between a private PBX system and a 2W telephone is converted to Ethernet messages using the optical Ethernet data communication network already deployed in the tactical weapon system. Thus, it is not necessary to install an additional optic cable for the ethernet telephone line and to maintain the private PSTN 2W telephone network. Also it provides safe and secure telecommunication operation under lightning and electromagnetic environments. This paper presents the conversion protocol from 2W telephone signals over Ethernet interface between PBX systems and 2W telephones, the mutual exchange protocol of ethernet messages between two converters, and the rule to process analog signal interface. Finally, we demonstrate that the proposed technique can provide a feasible solution in the tactical weapon system by analyzing its performance and experimental results such as the bandwidth of 2W telephone ethernet network and the transmission latency of voice signal, and the stability of optic ethernet voice network along with the ethernet data network.

• Journal of Service Research and Studies
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• v.12 no.2
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• pp.23-37
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• 2022

Based on the service scenario proposed by the existing Kim Tae-wan (2018) who can safely evacuate inmates with the help of a mobile application linked to a fire detection system in the event of a fire, the final purpose of this study is to develop the scenario by incorporating more realistic scenarios with mobile stimuli that can help them escape or act through the Delph In addition, to make the scenarios produced more realistic considering the structure and copper lines of a typical building, expert scenario verification and Delphi technique were applied to exclude unnecessary or impractical aspects of the existing scenarios. The results of the second Delphi survey showed that the primary psychology that could be seen at the time of the fire alarm were doubts, safety concerns and alarm, and the results of the second Delphi survey were analyzed, and the satisfaction of the content adequacy (CVR), convergence, and consensus was derived. Finally, this was applied to create a scenario in which a mobile application was assisted to evacuate the fire response phase. This study will allow the use of methods to increase the evacuation rate of those who are in the event of a fire.